V-REVCOMP: automated high-throughput detection of reverse complementary 16S rRNA gene sequences in large environmental and taxonomic datasets

Abstract Reverse complementary DNA sequences – sequences that are inadvertently given backwards with all purines and pyrimidines transposed – can affect sequence analysis detrimentally unless taken into account. We present an open-source, high-throughput software tool –v-revcomp (http://www.cmde.sci...

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Veröffentlicht in:	FEMS microbiology letters 2011-06, Vol.319 (2), p.140-145
Hauptverfasser:	Hartmann, Martin, Howes, Charles G., Veldre, Vilmar, Schneider, Salome, Vaishampayan, Parag A., Yannarell, Anthony C., Quince, Christopher, Johansson, Per, Björkroth, K. Johanna, Abarenkov, Kessy, Hallam, Steven J., Mohn, William W., Nilsson, R. Henrik
Format:	Artikel
Sprache:	eng
Schlagworte:	16S sequence Anomalies Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Biological and medical sciences Chimeras Complementary DNA Computer programs Databases, Nucleic Acid Datasets Deoxyribonucleic acid DNA Environmental Microbiology Fundamental and applied biological sciences. Psychology Gene sequencing Hidden Markov Models hmmer Microbiology Next-generation sequencing Nucleotide sequence Nucleotides Phylogeny Purines Pyrimidines Reliability analysis reverse complementary RNA, Ribosomal, 16S - genetics rRNA 16S Sequence Alignment Software Software development tools Source code SSU rRNA gene
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