An oral nervous necrosis virus vaccine using Vibrio anguillarum as an expression host provides early protection
Oral vaccination is the most desirable immunization method by which to prevent fish disease occurring at the early larval stage. We developed an Escherichia coli-based oral nervous necrosis virus (NNV) subunit vaccine and demonstrated its effectiveness in orange spotted grouper ( Epinephelus coioide...
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| Veröffentlicht in: | Aquaculture 2011-11, Vol.321 (1), p.26-33 |
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| creator | Chen, Yi-Min Shih, Ching-Hung Liu, Hsiao-Chien Wu, Cheng-Lung Lin, Chin-Chiu Wang, Han-Ching Chen, Tzong-Yueh Yang, Huey-Lang Lin, John Han-You |
| description | Oral vaccination is the most desirable immunization method by which to prevent fish disease occurring at the early larval stage. We developed an
Escherichia coli-based oral nervous necrosis virus (NNV) subunit vaccine and demonstrated its effectiveness in orange spotted grouper (
Epinephelus coioides) fry. However, the incubation period was determined to be over 2
weeks. To enhance the efficacy and shorten the incubation period, a safe and digestible biological adjuvant should be found.
Vibrio anguillarum, a common marine bacterium with immune-stimulatory capability was selected as an expression host. An expression vector containing its heat shock protein 60 gene (
groE) as an inducible promoter was constructed to express recombinant NNV coat protein as an antigen. The recombinant
V. anguillarum was inactivated and encapsulated in
Artemia nauplii to formulate a
V. anguillarum-based oral vaccine. Compared to the
E. coli-based oral vaccine, this novel oral vaccine not only elicited a higher expression of
Mx (encoding for an anti-viral protein) in the brain and viscera but also a higher specific antibody titer against NNV in the first week after inoculation. Meanwhile, a higher survival rate compared to an equal dosage of an
E. coli-based oral vaccine was also noticed in two individual challenge trials, indicating that an antigen expressed in an
V. anguillarum-based oral vaccine could enhance efficacy in a shorter incubation period and could reduce the risk of NNV infection.
► We development an oral nervous necrosis virus vaccine using
V. anguillarum as an expression host. ► The expression vector containing its heat shock protein 60 gene (
groE) as an inducible promoter. ► This novel oral vaccine elicited a higher expression of
Mx in the brain and viscera. ► It also a higher specific antibody titer against NNV in the first week after inoculation. ► The vaccine would shorter incubation period and reduce the risk of NNV infection. |
| doi_str_mv | 10.1016/j.aquaculture.2011.08.035 |
| format | Article |
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Escherichia coli-based oral nervous necrosis virus (NNV) subunit vaccine and demonstrated its effectiveness in orange spotted grouper (
Epinephelus coioides) fry. However, the incubation period was determined to be over 2
weeks. To enhance the efficacy and shorten the incubation period, a safe and digestible biological adjuvant should be found.
Vibrio anguillarum, a common marine bacterium with immune-stimulatory capability was selected as an expression host. An expression vector containing its heat shock protein 60 gene (
groE) as an inducible promoter was constructed to express recombinant NNV coat protein as an antigen. The recombinant
V. anguillarum was inactivated and encapsulated in
Artemia nauplii to formulate a
V. anguillarum-based oral vaccine. Compared to the
E. coli-based oral vaccine, this novel oral vaccine not only elicited a higher expression of
Mx (encoding for an anti-viral protein) in the brain and viscera but also a higher specific antibody titer against NNV in the first week after inoculation. Meanwhile, a higher survival rate compared to an equal dosage of an
E. coli-based oral vaccine was also noticed in two individual challenge trials, indicating that an antigen expressed in an
V. anguillarum-based oral vaccine could enhance efficacy in a shorter incubation period and could reduce the risk of NNV infection.
► We development an oral nervous necrosis virus vaccine using
V. anguillarum as an expression host. ► The expression vector containing its heat shock protein 60 gene (
groE) as an inducible promoter. ► This novel oral vaccine elicited a higher expression of
Mx in the brain and viscera. ► It also a higher specific antibody titer against NNV in the first week after inoculation. ► The vaccine would shorter incubation period and reduce the risk of NNV infection.</description><identifier>ISSN: 0044-8486</identifier><identifier>EISSN: 1873-5622</identifier><identifier>DOI: 10.1016/j.aquaculture.2011.08.035</identifier><identifier>CODEN: AQCLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adjuvant ; adjuvants ; Animal aquaculture ; Animal productions ; antibodies ; antigens ; Aquaculture ; Artemia ; bacteria ; Biological and medical sciences ; brain ; coat proteins ; Epinephelus coioides ; Escherichia ; Fish ; fish diseases ; Fundamental and applied biological sciences. Psychology ; Gene expression ; General aspects ; genes ; grouper ; heat shock proteins ; hosts ; Immunization ; incubation period ; Marine ; Microbiology ; nauplii ; necrosis ; oral vaccination ; Proteins ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; risk ; Subunit vaccine ; subunit vaccines ; survival rate ; Vibrio anguillarum ; Viral infections ; Virology ; viruses</subject><ispartof>Aquaculture, 2011-11, Vol.321 (1), p.26-33</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Elsevier Sequoia S.A. Nov 16, 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-c2bf4fbe2e4a9ae2a0ebb82c21b392bf1dc1f7fd24be9a5b09f9c89eb18854533</citedby><cites>FETCH-LOGICAL-c434t-c2bf4fbe2e4a9ae2a0ebb82c21b392bf1dc1f7fd24be9a5b09f9c89eb18854533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0044848611006831$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65308</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24627479$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Yi-Min</creatorcontrib><creatorcontrib>Shih, Ching-Hung</creatorcontrib><creatorcontrib>Liu, Hsiao-Chien</creatorcontrib><creatorcontrib>Wu, Cheng-Lung</creatorcontrib><creatorcontrib>Lin, Chin-Chiu</creatorcontrib><creatorcontrib>Wang, Han-Ching</creatorcontrib><creatorcontrib>Chen, Tzong-Yueh</creatorcontrib><creatorcontrib>Yang, Huey-Lang</creatorcontrib><creatorcontrib>Lin, John Han-You</creatorcontrib><title>An oral nervous necrosis virus vaccine using Vibrio anguillarum as an expression host provides early protection</title><title>Aquaculture</title><description>Oral vaccination is the most desirable immunization method by which to prevent fish disease occurring at the early larval stage. We developed an
Escherichia coli-based oral nervous necrosis virus (NNV) subunit vaccine and demonstrated its effectiveness in orange spotted grouper (
Epinephelus coioides) fry. However, the incubation period was determined to be over 2
weeks. To enhance the efficacy and shorten the incubation period, a safe and digestible biological adjuvant should be found.
Vibrio anguillarum, a common marine bacterium with immune-stimulatory capability was selected as an expression host. An expression vector containing its heat shock protein 60 gene (
groE) as an inducible promoter was constructed to express recombinant NNV coat protein as an antigen. The recombinant
V. anguillarum was inactivated and encapsulated in
Artemia nauplii to formulate a
V. anguillarum-based oral vaccine. Compared to the
E. coli-based oral vaccine, this novel oral vaccine not only elicited a higher expression of
Mx (encoding for an anti-viral protein) in the brain and viscera but also a higher specific antibody titer against NNV in the first week after inoculation. Meanwhile, a higher survival rate compared to an equal dosage of an
E. coli-based oral vaccine was also noticed in two individual challenge trials, indicating that an antigen expressed in an
V. anguillarum-based oral vaccine could enhance efficacy in a shorter incubation period and could reduce the risk of NNV infection.
► We development an oral nervous necrosis virus vaccine using
V. anguillarum as an expression host. ► The expression vector containing its heat shock protein 60 gene (
groE) as an inducible promoter. ► This novel oral vaccine elicited a higher expression of
Mx in the brain and viscera. ► It also a higher specific antibody titer against NNV in the first week after inoculation. ► The vaccine would shorter incubation period and reduce the risk of NNV infection.</description><subject>Adjuvant</subject><subject>adjuvants</subject><subject>Animal aquaculture</subject><subject>Animal productions</subject><subject>antibodies</subject><subject>antigens</subject><subject>Aquaculture</subject><subject>Artemia</subject><subject>bacteria</subject><subject>Biological and medical sciences</subject><subject>brain</subject><subject>coat proteins</subject><subject>Epinephelus coioides</subject><subject>Escherichia</subject><subject>Fish</subject><subject>fish diseases</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>General aspects</subject><subject>genes</subject><subject>grouper</subject><subject>heat shock proteins</subject><subject>hosts</subject><subject>Immunization</subject><subject>incubation period</subject><subject>Marine</subject><subject>Microbiology</subject><subject>nauplii</subject><subject>necrosis</subject><subject>oral vaccination</subject><subject>Proteins</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>risk</subject><subject>Subunit vaccine</subject><subject>subunit vaccines</subject><subject>survival rate</subject><subject>Vibrio anguillarum</subject><subject>Viral infections</subject><subject>Virology</subject><subject>viruses</subject><issn>0044-8486</issn><issn>1873-5622</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqNkV-L1TAQxYsoeF39DEZBfGqdpGmbPC6X9Q8s-KDra5im02suvcndpC3utzflLiI--TQM88uZkzlF8YZDxYG3H44V3i9ol2leIlUCOK9AVVA3T4odV11dNq0QT4sdgJSlkqp9XrxI6QgAbdvwXRGuPQsRJ-YprmFJudoYkktsdTG3K1rrPLElOX9gP1wfXWDoD4ubJozLiWHKLaNf50gpueDZz5Bmdo5hdQMlRhinh62dyc55_LJ4NuKU6NVjvSruPt58338ub79--rK_vi2trOVcWtGPcuxJkESNJBCo75Wwgve1zjM-WD524yBkTxqbHvSordLUc6Ua2dT1VfH-optX3y-UZnNyyVI27Sl_02gQdaclqEy-_Yc8hiX6bM4o3UHT6rbLkL5A23FSpNGcozthfDAczBaEOZq_gjBbEAaUyUHkt-8eF2CyOI0RvXXpj4CQrehkpzP3-sKNGAweYmbuvmUhCVlfNrC52F8IypdbHUWTrCNvaXAxn9cMwf2Hn98qQ7FT</recordid><startdate>20111116</startdate><enddate>20111116</enddate><creator>Chen, Yi-Min</creator><creator>Shih, Ching-Hung</creator><creator>Liu, Hsiao-Chien</creator><creator>Wu, Cheng-Lung</creator><creator>Lin, Chin-Chiu</creator><creator>Wang, Han-Ching</creator><creator>Chen, Tzong-Yueh</creator><creator>Yang, Huey-Lang</creator><creator>Lin, John Han-You</creator><general>Elsevier B.V</general><general>Elsevier</general><general>Elsevier Sequoia S.A</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7ST</scope><scope>7TN</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>20111116</creationdate><title>An oral nervous necrosis virus vaccine using Vibrio anguillarum as an expression host provides early protection</title><author>Chen, Yi-Min ; Shih, Ching-Hung ; Liu, Hsiao-Chien ; Wu, Cheng-Lung ; Lin, Chin-Chiu ; Wang, Han-Ching ; Chen, Tzong-Yueh ; Yang, Huey-Lang ; Lin, John Han-You</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-c2bf4fbe2e4a9ae2a0ebb82c21b392bf1dc1f7fd24be9a5b09f9c89eb18854533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adjuvant</topic><topic>adjuvants</topic><topic>Animal aquaculture</topic><topic>Animal productions</topic><topic>antibodies</topic><topic>antigens</topic><topic>Aquaculture</topic><topic>Artemia</topic><topic>bacteria</topic><topic>Biological and medical sciences</topic><topic>brain</topic><topic>coat proteins</topic><topic>Epinephelus coioides</topic><topic>Escherichia</topic><topic>Fish</topic><topic>fish diseases</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>General aspects</topic><topic>genes</topic><topic>grouper</topic><topic>heat shock proteins</topic><topic>hosts</topic><topic>Immunization</topic><topic>incubation period</topic><topic>Marine</topic><topic>Microbiology</topic><topic>nauplii</topic><topic>necrosis</topic><topic>oral vaccination</topic><topic>Proteins</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>risk</topic><topic>Subunit vaccine</topic><topic>subunit vaccines</topic><topic>survival rate</topic><topic>Vibrio anguillarum</topic><topic>Viral infections</topic><topic>Virology</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Yi-Min</creatorcontrib><creatorcontrib>Shih, Ching-Hung</creatorcontrib><creatorcontrib>Liu, Hsiao-Chien</creatorcontrib><creatorcontrib>Wu, Cheng-Lung</creatorcontrib><creatorcontrib>Lin, Chin-Chiu</creatorcontrib><creatorcontrib>Wang, Han-Ching</creatorcontrib><creatorcontrib>Chen, Tzong-Yueh</creatorcontrib><creatorcontrib>Yang, Huey-Lang</creatorcontrib><creatorcontrib>Lin, John Han-You</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Aquaculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Yi-Min</au><au>Shih, Ching-Hung</au><au>Liu, Hsiao-Chien</au><au>Wu, Cheng-Lung</au><au>Lin, Chin-Chiu</au><au>Wang, Han-Ching</au><au>Chen, Tzong-Yueh</au><au>Yang, Huey-Lang</au><au>Lin, John Han-You</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An oral nervous necrosis virus vaccine using Vibrio anguillarum as an expression host provides early protection</atitle><jtitle>Aquaculture</jtitle><date>2011-11-16</date><risdate>2011</risdate><volume>321</volume><issue>1</issue><spage>26</spage><epage>33</epage><pages>26-33</pages><issn>0044-8486</issn><eissn>1873-5622</eissn><coden>AQCLAL</coden><abstract>Oral vaccination is the most desirable immunization method by which to prevent fish disease occurring at the early larval stage. We developed an
Escherichia coli-based oral nervous necrosis virus (NNV) subunit vaccine and demonstrated its effectiveness in orange spotted grouper (
Epinephelus coioides) fry. However, the incubation period was determined to be over 2
weeks. To enhance the efficacy and shorten the incubation period, a safe and digestible biological adjuvant should be found.
Vibrio anguillarum, a common marine bacterium with immune-stimulatory capability was selected as an expression host. An expression vector containing its heat shock protein 60 gene (
groE) as an inducible promoter was constructed to express recombinant NNV coat protein as an antigen. The recombinant
V. anguillarum was inactivated and encapsulated in
Artemia nauplii to formulate a
V. anguillarum-based oral vaccine. Compared to the
E. coli-based oral vaccine, this novel oral vaccine not only elicited a higher expression of
Mx (encoding for an anti-viral protein) in the brain and viscera but also a higher specific antibody titer against NNV in the first week after inoculation. Meanwhile, a higher survival rate compared to an equal dosage of an
E. coli-based oral vaccine was also noticed in two individual challenge trials, indicating that an antigen expressed in an
V. anguillarum-based oral vaccine could enhance efficacy in a shorter incubation period and could reduce the risk of NNV infection.
► We development an oral nervous necrosis virus vaccine using
V. anguillarum as an expression host. ► The expression vector containing its heat shock protein 60 gene (
groE) as an inducible promoter. ► This novel oral vaccine elicited a higher expression of
Mx in the brain and viscera. ► It also a higher specific antibody titer against NNV in the first week after inoculation. ► The vaccine would shorter incubation period and reduce the risk of NNV infection.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.aquaculture.2011.08.035</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Aquaculture, 2011-11, Vol.321 (1), p.26-33 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Adjuvant adjuvants Animal aquaculture Animal productions antibodies antigens Aquaculture Artemia bacteria Biological and medical sciences brain coat proteins Epinephelus coioides Escherichia Fish fish diseases Fundamental and applied biological sciences. Psychology Gene expression General aspects genes grouper heat shock proteins hosts Immunization incubation period Marine Microbiology nauplii necrosis oral vaccination Proteins Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains risk Subunit vaccine subunit vaccines survival rate Vibrio anguillarum Viral infections Virology viruses |
| title | An oral nervous necrosis virus vaccine using Vibrio anguillarum as an expression host provides early protection |
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