Comprehension of terminal differentiation and dedifferentiation of chondrocytes during passage cultures
A high density collagen type I coated substrate (CL substrate) was used to evaluate the chondrocyte phenotypes in passaged cultures. With increasing age of cell population (population doubling ( PD) = 0–14.5), the frequency of non-dividing spindle shaped cells without ALP activity increased, accompa...
Gespeichert in:
| Veröffentlicht in: | Journal of bioscience and bioengineering 2011-10, Vol.112 (4), p.395-401 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 401 |
|---|---|
| container_issue | 4 |
| container_start_page | 395 |
| container_title | Journal of bioscience and bioengineering |
| container_volume | 112 |
| creator | Nadzir, Masrina Mohd Kino-oka, Masahiro Maruyama, Nao Sato, Yasuaki Kim, Mee-Hae Sugawara, Katsura Taya, Masahito |
| description | A high density collagen type I coated substrate (CL substrate) was used to evaluate the chondrocyte phenotypes in passaged cultures. With increasing age of cell population (population doubling (
PD)
=
0–14.5), the frequency of non-dividing spindle shaped cells without ALP activity increased, accompanied with an increase in gene expression of collagen type I, meaning the senescence of dedifferentiated cells. At the middle age of cell population (
PD
=
5.1 and 6.6), the high frequency of polygonal shaped cells with ALP activity existed on the CL substrate together with up-regulated expressions of collagen types II and X, indicating the terminal differentiation of chondrocytes. When the chondrocytes passaged up to the middle age were embedded in collagen gel, the high frequency of single hypertrophic cells with collagen type II formation was recognized, which supports the thought that the high gene expression of collagen type II was attributed to terminal differentiation rather than redifferentiation. These results show that the CL substrate can draw out the potential of terminal differentiation in chondrocytes, which is unattainable by a polystyrene surface, and that the CL substrate can be a tool to evaluate cell quality in three-dimensional culture with the collagen gel. |
| doi_str_mv | 10.1016/j.jbiosc.2011.06.005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_902379031</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1389172311002271</els_id><sourcerecordid>898837955</sourcerecordid><originalsourceid>FETCH-LOGICAL-c563t-b480990ff583bd98bbb47abf7ee90634873a335fbd8c45430c3057e1e53d9e413</originalsourceid><addsrcrecordid>eNqFkU2L1TAUhosozof-A9FuxNWtJ03aJBtBLn7BgAuddUiTkzu5tMk1aQfm35vSq4ILXSXkPG_O4TxV9YJAQ4D0b4_NcfAxm6YFQhroG4DuUXVJKOM7xlryeL0LuSO8pRfVVc5HAMKBk6fVRUs4F4TAZXXYx-mU8A5D9jHU0dUzpskHPdbWO4cJw-z1vNZ0sLXFv19LwtzFYFM0DzPm2i7Jh0N90jnrA9ZmGeclYX5WPXF6zPj8fF5Xtx8_fN9_3t18_fRl__5mZ7qezruBCZASnOsEHawUwzAwrgfHESX0lAlONaWdG6wwrGMUDIWOI8GOWomM0OvqzfbvKcUfC-ZZTT4bHEcdMC5ZSWgpl0D_TwopREG7rpBsI02KOSd06pT8pNODIqBWF-qoNhdqdaGgV8VFib08N1iGCe3v0K_lF-D1GdDZ6NElHYzPfzjGWyjjFu7VxjkdlT6kwtx-K516WMs9yEK82wgsq733mFQ2HoMpthKaWdno_z3rT9XltB8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>898837955</pqid></control><display><type>article</type><title>Comprehension of terminal differentiation and dedifferentiation of chondrocytes during passage cultures</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Nadzir, Masrina Mohd ; Kino-oka, Masahiro ; Maruyama, Nao ; Sato, Yasuaki ; Kim, Mee-Hae ; Sugawara, Katsura ; Taya, Masahito</creator><creatorcontrib>Nadzir, Masrina Mohd ; Kino-oka, Masahiro ; Maruyama, Nao ; Sato, Yasuaki ; Kim, Mee-Hae ; Sugawara, Katsura ; Taya, Masahito</creatorcontrib><description>A high density collagen type I coated substrate (CL substrate) was used to evaluate the chondrocyte phenotypes in passaged cultures. With increasing age of cell population (population doubling (
PD)
=
0–14.5), the frequency of non-dividing spindle shaped cells without ALP activity increased, accompanied with an increase in gene expression of collagen type I, meaning the senescence of dedifferentiated cells. At the middle age of cell population (
PD
=
5.1 and 6.6), the high frequency of polygonal shaped cells with ALP activity existed on the CL substrate together with up-regulated expressions of collagen types II and X, indicating the terminal differentiation of chondrocytes. When the chondrocytes passaged up to the middle age were embedded in collagen gel, the high frequency of single hypertrophic cells with collagen type II formation was recognized, which supports the thought that the high gene expression of collagen type II was attributed to terminal differentiation rather than redifferentiation. These results show that the CL substrate can draw out the potential of terminal differentiation in chondrocytes, which is unattainable by a polystyrene surface, and that the CL substrate can be a tool to evaluate cell quality in three-dimensional culture with the collagen gel.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1016/j.jbiosc.2011.06.005</identifier><identifier>PMID: 21778110</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biotechnology ; Cell Culture Techniques ; Cell Dedifferentiation ; Cell Differentiation ; Cells, Cultured ; chondrocytes ; Chondrocytes - cytology ; Chondrocytes - metabolism ; collagen ; Collagen - biosynthesis ; Collagen - metabolism ; Collagen Type I - metabolism ; Collagen Type II - biosynthesis ; Collagen-coated substrate ; Cultured cartilage ; Extracellular Matrix - metabolism ; Fundamental and applied biological sciences. Psychology ; gels ; gene expression ; Humans ; Passage cultures ; phenotype ; polystyrenes ; Rabbit chondrocytes ; Terminal differentiation ; Tissue engineering</subject><ispartof>Journal of bioscience and bioengineering, 2011-10, Vol.112 (4), p.395-401</ispartof><rights>2011 The Society for Biotechnology, Japan</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c563t-b480990ff583bd98bbb47abf7ee90634873a335fbd8c45430c3057e1e53d9e413</citedby><cites>FETCH-LOGICAL-c563t-b480990ff583bd98bbb47abf7ee90634873a335fbd8c45430c3057e1e53d9e413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1389172311002271$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24720023$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21778110$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nadzir, Masrina Mohd</creatorcontrib><creatorcontrib>Kino-oka, Masahiro</creatorcontrib><creatorcontrib>Maruyama, Nao</creatorcontrib><creatorcontrib>Sato, Yasuaki</creatorcontrib><creatorcontrib>Kim, Mee-Hae</creatorcontrib><creatorcontrib>Sugawara, Katsura</creatorcontrib><creatorcontrib>Taya, Masahito</creatorcontrib><title>Comprehension of terminal differentiation and dedifferentiation of chondrocytes during passage cultures</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>A high density collagen type I coated substrate (CL substrate) was used to evaluate the chondrocyte phenotypes in passaged cultures. With increasing age of cell population (population doubling (
PD)
=
0–14.5), the frequency of non-dividing spindle shaped cells without ALP activity increased, accompanied with an increase in gene expression of collagen type I, meaning the senescence of dedifferentiated cells. At the middle age of cell population (
PD
=
5.1 and 6.6), the high frequency of polygonal shaped cells with ALP activity existed on the CL substrate together with up-regulated expressions of collagen types II and X, indicating the terminal differentiation of chondrocytes. When the chondrocytes passaged up to the middle age were embedded in collagen gel, the high frequency of single hypertrophic cells with collagen type II formation was recognized, which supports the thought that the high gene expression of collagen type II was attributed to terminal differentiation rather than redifferentiation. These results show that the CL substrate can draw out the potential of terminal differentiation in chondrocytes, which is unattainable by a polystyrene surface, and that the CL substrate can be a tool to evaluate cell quality in three-dimensional culture with the collagen gel.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Culture Techniques</subject><subject>Cell Dedifferentiation</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>chondrocytes</subject><subject>Chondrocytes - cytology</subject><subject>Chondrocytes - metabolism</subject><subject>collagen</subject><subject>Collagen - biosynthesis</subject><subject>Collagen - metabolism</subject><subject>Collagen Type I - metabolism</subject><subject>Collagen Type II - biosynthesis</subject><subject>Collagen-coated substrate</subject><subject>Cultured cartilage</subject><subject>Extracellular Matrix - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gels</subject><subject>gene expression</subject><subject>Humans</subject><subject>Passage cultures</subject><subject>phenotype</subject><subject>polystyrenes</subject><subject>Rabbit chondrocytes</subject><subject>Terminal differentiation</subject><subject>Tissue engineering</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2L1TAUhosozof-A9FuxNWtJ03aJBtBLn7BgAuddUiTkzu5tMk1aQfm35vSq4ILXSXkPG_O4TxV9YJAQ4D0b4_NcfAxm6YFQhroG4DuUXVJKOM7xlryeL0LuSO8pRfVVc5HAMKBk6fVRUs4F4TAZXXYx-mU8A5D9jHU0dUzpskHPdbWO4cJw-z1vNZ0sLXFv19LwtzFYFM0DzPm2i7Jh0N90jnrA9ZmGeclYX5WPXF6zPj8fF5Xtx8_fN9_3t18_fRl__5mZ7qezruBCZASnOsEHawUwzAwrgfHESX0lAlONaWdG6wwrGMUDIWOI8GOWomM0OvqzfbvKcUfC-ZZTT4bHEcdMC5ZSWgpl0D_TwopREG7rpBsI02KOSd06pT8pNODIqBWF-qoNhdqdaGgV8VFib08N1iGCe3v0K_lF-D1GdDZ6NElHYzPfzjGWyjjFu7VxjkdlT6kwtx-K516WMs9yEK82wgsq733mFQ2HoMpthKaWdno_z3rT9XltB8</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Nadzir, Masrina Mohd</creator><creator>Kino-oka, Masahiro</creator><creator>Maruyama, Nao</creator><creator>Sato, Yasuaki</creator><creator>Kim, Mee-Hae</creator><creator>Sugawara, Katsura</creator><creator>Taya, Masahito</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20111001</creationdate><title>Comprehension of terminal differentiation and dedifferentiation of chondrocytes during passage cultures</title><author>Nadzir, Masrina Mohd ; Kino-oka, Masahiro ; Maruyama, Nao ; Sato, Yasuaki ; Kim, Mee-Hae ; Sugawara, Katsura ; Taya, Masahito</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c563t-b480990ff583bd98bbb47abf7ee90634873a335fbd8c45430c3057e1e53d9e413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Culture Techniques</topic><topic>Cell Dedifferentiation</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>chondrocytes</topic><topic>Chondrocytes - cytology</topic><topic>Chondrocytes - metabolism</topic><topic>collagen</topic><topic>Collagen - biosynthesis</topic><topic>Collagen - metabolism</topic><topic>Collagen Type I - metabolism</topic><topic>Collagen Type II - biosynthesis</topic><topic>Collagen-coated substrate</topic><topic>Cultured cartilage</topic><topic>Extracellular Matrix - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gels</topic><topic>gene expression</topic><topic>Humans</topic><topic>Passage cultures</topic><topic>phenotype</topic><topic>polystyrenes</topic><topic>Rabbit chondrocytes</topic><topic>Terminal differentiation</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nadzir, Masrina Mohd</creatorcontrib><creatorcontrib>Kino-oka, Masahiro</creatorcontrib><creatorcontrib>Maruyama, Nao</creatorcontrib><creatorcontrib>Sato, Yasuaki</creatorcontrib><creatorcontrib>Kim, Mee-Hae</creatorcontrib><creatorcontrib>Sugawara, Katsura</creatorcontrib><creatorcontrib>Taya, Masahito</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nadzir, Masrina Mohd</au><au>Kino-oka, Masahiro</au><au>Maruyama, Nao</au><au>Sato, Yasuaki</au><au>Kim, Mee-Hae</au><au>Sugawara, Katsura</au><au>Taya, Masahito</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comprehension of terminal differentiation and dedifferentiation of chondrocytes during passage cultures</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>112</volume><issue>4</issue><spage>395</spage><epage>401</epage><pages>395-401</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>A high density collagen type I coated substrate (CL substrate) was used to evaluate the chondrocyte phenotypes in passaged cultures. With increasing age of cell population (population doubling (
PD)
=
0–14.5), the frequency of non-dividing spindle shaped cells without ALP activity increased, accompanied with an increase in gene expression of collagen type I, meaning the senescence of dedifferentiated cells. At the middle age of cell population (
PD
=
5.1 and 6.6), the high frequency of polygonal shaped cells with ALP activity existed on the CL substrate together with up-regulated expressions of collagen types II and X, indicating the terminal differentiation of chondrocytes. When the chondrocytes passaged up to the middle age were embedded in collagen gel, the high frequency of single hypertrophic cells with collagen type II formation was recognized, which supports the thought that the high gene expression of collagen type II was attributed to terminal differentiation rather than redifferentiation. These results show that the CL substrate can draw out the potential of terminal differentiation in chondrocytes, which is unattainable by a polystyrene surface, and that the CL substrate can be a tool to evaluate cell quality in three-dimensional culture with the collagen gel.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21778110</pmid><doi>10.1016/j.jbiosc.2011.06.005</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1389-1723 |
| ispartof | Journal of bioscience and bioengineering, 2011-10, Vol.112 (4), p.395-401 |
| issn | 1389-1723 1347-4421 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_902379031 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Biotechnology Cell Culture Techniques Cell Dedifferentiation Cell Differentiation Cells, Cultured chondrocytes Chondrocytes - cytology Chondrocytes - metabolism collagen Collagen - biosynthesis Collagen - metabolism Collagen Type I - metabolism Collagen Type II - biosynthesis Collagen-coated substrate Cultured cartilage Extracellular Matrix - metabolism Fundamental and applied biological sciences. Psychology gels gene expression Humans Passage cultures phenotype polystyrenes Rabbit chondrocytes Terminal differentiation Tissue engineering |
| title | Comprehension of terminal differentiation and dedifferentiation of chondrocytes during passage cultures |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T16%3A27%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comprehension%20of%20terminal%20differentiation%20and%20dedifferentiation%20of%20chondrocytes%20during%20passage%20cultures&rft.jtitle=Journal%20of%20bioscience%20and%20bioengineering&rft.au=Nadzir,%20Masrina%20Mohd&rft.date=2011-10-01&rft.volume=112&rft.issue=4&rft.spage=395&rft.epage=401&rft.pages=395-401&rft.issn=1389-1723&rft.eissn=1347-4421&rft_id=info:doi/10.1016/j.jbiosc.2011.06.005&rft_dat=%3Cproquest_cross%3E898837955%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=898837955&rft_id=info:pmid/21778110&rft_els_id=S1389172311002271&rfr_iscdi=true |