effects of genotype, inflorescence developmental stage and induction medium on callus induction and plant regeneration in two Miscanthus species
Several grass species of the genus Miscanthus are considered to be outstanding candidates for a sustainable production of biomass to generate renewable energy. The purpose of this study was to investigate the effects of genotype, the developmental stage of the explant donor inflorescence and the ind...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2010-07, Vol.102 (1), p.79-86 |
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| description | Several grass species of the genus Miscanthus are considered to be outstanding candidates for a sustainable production of biomass to generate renewable energy. The purpose of this study was to investigate the effects of genotype, the developmental stage of the explant donor inflorescence and the induction medium on the success rate of micropropagation. The experiments were conducted on three genotypes of M. sinensis and one of M. x giganteus. Explants from the youngest inflorescences (0.1-2.5 cm in length) showed a significantly higher callus induction rate than those from more developed inflorescences (2.6-5 cm in length). In addition, cultures initiated from explants from the youngest inflorescences showed significantly the highest rates of callus regeneration and the highest shoot regeneration rate. Three out of the four genotypes tested showed the best shoot regeneration from calli initiated from the youngest inflorescences when cultured on the Murashige and Skoog basal medium (MS) with 5 mg l⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1 mg l⁻¹ 6-benzyladenine (BA). The percentages of calli from those genotypes showing regeneration ranged from 45 to 76.7%, and the corresponding shoot regeneration rates ranged from 1.85 to 6.33 shoots/callus. This demonstrates that, with some adjustments, efficient micropropagation of Miscanthus sp. is feasible. |
| doi_str_mv | 10.1007/s11240-010-9708-6 |
| format | Article |
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The purpose of this study was to investigate the effects of genotype, the developmental stage of the explant donor inflorescence and the induction medium on the success rate of micropropagation. The experiments were conducted on three genotypes of M. sinensis and one of M. x giganteus. Explants from the youngest inflorescences (0.1-2.5 cm in length) showed a significantly higher callus induction rate than those from more developed inflorescences (2.6-5 cm in length). In addition, cultures initiated from explants from the youngest inflorescences showed significantly the highest rates of callus regeneration and the highest shoot regeneration rate. Three out of the four genotypes tested showed the best shoot regeneration from calli initiated from the youngest inflorescences when cultured on the Murashige and Skoog basal medium (MS) with 5 mg l⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1 mg l⁻¹ 6-benzyladenine (BA). The percentages of calli from those genotypes showing regeneration ranged from 45 to 76.7%, and the corresponding shoot regeneration rates ranged from 1.85 to 6.33 shoots/callus. This demonstrates that, with some adjustments, efficient micropropagation of Miscanthus sp. is feasible.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-010-9708-6</identifier><identifier>CODEN: PTCEDJ</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>2,4-D ; Benzyladenine ; Biological and medical sciences ; biomass ; Biomass energy production ; Biomedical and Life Sciences ; Biotechnology ; Callus ; culture media ; Developmental stages ; Dichlorophenoxyacetic acid ; energy crops ; Explants ; Fundamental and applied biological sciences. Psychology ; genotype ; Genotypes ; in vitro regeneration ; inflorescences ; Life Sciences ; Micropropagation ; Miscanthus ; Miscanthus sinensis ; Miscanthus x giganteus ; Original Paper ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Regeneration ; Renewable energy ; Shoots ; Sustainable production ; tissue culture</subject><ispartof>Plant cell, tissue and organ culture, 2010-07, Vol.102 (1), p.79-86</ispartof><rights>Springer Science+Business Media B.V. 2010</rights><rights>2015 INIST-CNRS</rights><rights>Plant Cell, Tissue and Organ Culture (PCTOC) is a copyright of Springer, (2010). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-f7290502f63647c5c9f44d1a4f28f270f6192996efa37c822b594d755ecac7c03</citedby><cites>FETCH-LOGICAL-c468t-f7290502f63647c5c9f44d1a4f28f270f6192996efa37c822b594d755ecac7c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-010-9708-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-010-9708-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22890468$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Głowacka, K</creatorcontrib><creatorcontrib>Jeżowski, S</creatorcontrib><creatorcontrib>Kaczmarek, Z</creatorcontrib><title>effects of genotype, inflorescence developmental stage and induction medium on callus induction and plant regeneration in two Miscanthus species</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>Several grass species of the genus Miscanthus are considered to be outstanding candidates for a sustainable production of biomass to generate renewable energy. The purpose of this study was to investigate the effects of genotype, the developmental stage of the explant donor inflorescence and the induction medium on the success rate of micropropagation. The experiments were conducted on three genotypes of M. sinensis and one of M. x giganteus. Explants from the youngest inflorescences (0.1-2.5 cm in length) showed a significantly higher callus induction rate than those from more developed inflorescences (2.6-5 cm in length). In addition, cultures initiated from explants from the youngest inflorescences showed significantly the highest rates of callus regeneration and the highest shoot regeneration rate. Three out of the four genotypes tested showed the best shoot regeneration from calli initiated from the youngest inflorescences when cultured on the Murashige and Skoog basal medium (MS) with 5 mg l⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1 mg l⁻¹ 6-benzyladenine (BA). The percentages of calli from those genotypes showing regeneration ranged from 45 to 76.7%, and the corresponding shoot regeneration rates ranged from 1.85 to 6.33 shoots/callus. This demonstrates that, with some adjustments, efficient micropropagation of Miscanthus sp. is feasible.</description><subject>2,4-D</subject><subject>Benzyladenine</subject><subject>Biological and medical sciences</subject><subject>biomass</subject><subject>Biomass energy production</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Callus</subject><subject>culture media</subject><subject>Developmental stages</subject><subject>Dichlorophenoxyacetic acid</subject><subject>energy crops</subject><subject>Explants</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genotype</subject><subject>Genotypes</subject><subject>in vitro regeneration</subject><subject>inflorescences</subject><subject>Life Sciences</subject><subject>Micropropagation</subject><subject>Miscanthus</subject><subject>Miscanthus sinensis</subject><subject>Miscanthus x giganteus</subject><subject>Original Paper</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Regeneration</subject><subject>Renewable energy</subject><subject>Shoots</subject><subject>Sustainable production</subject><subject>tissue culture</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kc2OFSEUhDtGE6-jD-BKEmPc2Hqg-V2aiX_JGBc6a4L04dqTbmiB1sxb-Mhy7YkaF64gnK-Kguq6hxSeUwD1olDKOPRAoTcKdC9vdQcq1NAL4Px2dwAqVS-1UHe7e6VcAYAcOD10PzAE9LWQFMgRY6rXKz4jUwxzylg8Ro9kxG84p3XBWN1MSnVHJC6OjRo3X6cUyYLjtC2k7byb5638NTqB6-xiJRnbBZjdr-Mpkvo9kfdT8W32pUnKin7Ccr-7E9xc8MHNetZdvn716fxtf_Hhzbvzlxe951LXPihmQAALcpBceeFN4HykjgemA1MQJDXMGInBDcprxj4Lw0clBHrnlYfhrHu6-645fd2wVLu0LDi3qJi2Yg2wQWmjTSMf_0NepS3HFs4yJsyglRh4o-hO-ZxKyRjsmqfF5WtLwZ4qsntFtlVkTxVZ2TRPbpxd-4Y5ZBf9VH4LGdMG2msbx3autFE8Yv6T4H_mj3ZRcMm6Y27Glx8Z0AGoFvwU_Cepwqwy</recordid><startdate>20100701</startdate><enddate>20100701</enddate><creator>Głowacka, K</creator><creator>Jeżowski, S</creator><creator>Kaczmarek, Z</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100701</creationdate><title>effects of genotype, inflorescence developmental stage and induction medium on callus induction and plant regeneration in two Miscanthus species</title><author>Głowacka, K ; Jeżowski, S ; Kaczmarek, Z</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-f7290502f63647c5c9f44d1a4f28f270f6192996efa37c822b594d755ecac7c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>2,4-D</topic><topic>Benzyladenine</topic><topic>Biological and medical sciences</topic><topic>biomass</topic><topic>Biomass energy production</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Callus</topic><topic>culture media</topic><topic>Developmental stages</topic><topic>Dichlorophenoxyacetic acid</topic><topic>energy crops</topic><topic>Explants</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genotype</topic><topic>Genotypes</topic><topic>in vitro regeneration</topic><topic>inflorescences</topic><topic>Life Sciences</topic><topic>Micropropagation</topic><topic>Miscanthus</topic><topic>Miscanthus sinensis</topic><topic>Miscanthus x giganteus</topic><topic>Original Paper</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Regeneration</topic><topic>Renewable energy</topic><topic>Shoots</topic><topic>Sustainable production</topic><topic>tissue culture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Głowacka, K</creatorcontrib><creatorcontrib>Jeżowski, S</creatorcontrib><creatorcontrib>Kaczmarek, Z</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Głowacka, K</au><au>Jeżowski, S</au><au>Kaczmarek, Z</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>effects of genotype, inflorescence developmental stage and induction medium on callus induction and plant regeneration in two Miscanthus species</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2010-07-01</date><risdate>2010</risdate><volume>102</volume><issue>1</issue><spage>79</spage><epage>86</epage><pages>79-86</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><coden>PTCEDJ</coden><abstract>Several grass species of the genus Miscanthus are considered to be outstanding candidates for a sustainable production of biomass to generate renewable energy. The purpose of this study was to investigate the effects of genotype, the developmental stage of the explant donor inflorescence and the induction medium on the success rate of micropropagation. The experiments were conducted on three genotypes of M. sinensis and one of M. x giganteus. Explants from the youngest inflorescences (0.1-2.5 cm in length) showed a significantly higher callus induction rate than those from more developed inflorescences (2.6-5 cm in length). In addition, cultures initiated from explants from the youngest inflorescences showed significantly the highest rates of callus regeneration and the highest shoot regeneration rate. Three out of the four genotypes tested showed the best shoot regeneration from calli initiated from the youngest inflorescences when cultured on the Murashige and Skoog basal medium (MS) with 5 mg l⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1 mg l⁻¹ 6-benzyladenine (BA). The percentages of calli from those genotypes showing regeneration ranged from 45 to 76.7%, and the corresponding shoot regeneration rates ranged from 1.85 to 6.33 shoots/callus. This demonstrates that, with some adjustments, efficient micropropagation of Miscanthus sp. is feasible.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><doi>10.1007/s11240-010-9708-6</doi><tpages>8</tpages></addata></record> |
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| ispartof | Plant cell, tissue and organ culture, 2010-07, Vol.102 (1), p.79-86 |
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| subjects | 2,4-D Benzyladenine Biological and medical sciences biomass Biomass energy production Biomedical and Life Sciences Biotechnology Callus culture media Developmental stages Dichlorophenoxyacetic acid energy crops Explants Fundamental and applied biological sciences. Psychology genotype Genotypes in vitro regeneration inflorescences Life Sciences Micropropagation Miscanthus Miscanthus sinensis Miscanthus x giganteus Original Paper Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Regeneration Renewable energy Shoots Sustainable production tissue culture |
| title | effects of genotype, inflorescence developmental stage and induction medium on callus induction and plant regeneration in two Miscanthus species |
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