One-step homogeneous C-reactive protein assay for saliva
Cardiovascular disease is the leading cause of death in the world. Human C-reactive protein (CRP) has been used in the risk assessment of coronary events. Human saliva mirrors the body's health and well-being and is non-invasive, easy to collect and ideal for third world countries as well as fo...
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| Veröffentlicht in: | Journal of immunological methods 2011-10, Vol.373 (1-2), p.19-25 |
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| creator | Punyadeera, Chamindie Dimeski, Goce Kostner, Karam Beyerlein, Peter Cooper-White, Justin |
| description | Cardiovascular disease is the leading cause of death in the world. Human C-reactive protein (CRP) has been used in the risk assessment of coronary events. Human saliva mirrors the body's health and well-being and is non-invasive, easy to collect and ideal for third world countries as well as for large patient screening. The aim was to establish a saliva CRP reference range and to demonstrate the clinical utility of salivary CRP levels in assessing the coronary events in a primary health care setting.
We have used a homogeneous bead based assay to detect CRP levels in human saliva. We have developed a rapid 15min (vs 90min), sequential, one-step assay to detect CRP in saliva. Saliva was collected from healthy volunteers (n=55, ages 20–70years) as well as from cardiac patients (n=28, ages 43–86years).
The assay incubation time was optimised from 90min to 15min and generated a positive correlation (n=29, range 10–2189pg/mL, r2=0.94; Passing Bablok slope 0.885, Intercept 0, p>0.10), meaning we could decrease the incubation time and produce equivalent results with confidence. The mean CRP level in the saliva of healthy human volunteers was 285pg/mL and in cardiac patients was 1680pg/mL (p |
| doi_str_mv | 10.1016/j.jim.2011.07.013 |
| format | Article |
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We have used a homogeneous bead based assay to detect CRP levels in human saliva. We have developed a rapid 15min (vs 90min), sequential, one-step assay to detect CRP in saliva. Saliva was collected from healthy volunteers (n=55, ages 20–70years) as well as from cardiac patients (n=28, ages 43–86years).
The assay incubation time was optimised from 90min to 15min and generated a positive correlation (n=29, range 10–2189pg/mL, r2=0.94; Passing Bablok slope 0.885, Intercept 0, p>0.10), meaning we could decrease the incubation time and produce equivalent results with confidence. The mean CRP level in the saliva of healthy human volunteers was 285pg/mL and in cardiac patients was 1680pg/mL (p<0.01). Analysis of CRP concentrations in paired serum and saliva samples from cardiac patients gave a positive correlation (r2=0.84, p<0.001) and the salivary CRP concentration capable of distinguishing healthy from diseased patients.
The results suggest that this minimally invasive, rapid and sensitive assay will be useful in large patient screening studies for risk assessment of coronary events.
► We have developed 15-min salivary CRP assay compatible for near patient testing. ► Salivary CRP levels are significantly higher in cardiac patients compared to healthy individuals. ► Salivary CRP levels are useful in risk assessment of coronary events.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/j.jim.2011.07.013</identifier><identifier>PMID: 21821037</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adolescent ; Adult ; Aged, 80 and over ; Biological and medical sciences ; blood serum ; C-reactive protein ; C-Reactive Protein - analysis ; cardiovascular diseases ; Cardiovascular Diseases - blood ; Cardiovascular Diseases - diagnosis ; Cardiovascular Diseases - metabolism ; Cardiovascular risk factors ; death ; developing countries ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; health services ; High-sensitive C-reactive protein assay ; Homogeneous bead based assay ; Human saliva ; Humans ; Mass Screening - methods ; Middle Aged ; Molecular immunology ; Non-invasive ; patients ; Reference Values ; Reproducibility of Results ; risk assessment ; Risk Assessment - methods ; Risk Factors ; saliva ; Saliva - chemistry ; screening ; Sensitivity and Specificity ; Techniques ; volunteers ; Young Adult</subject><ispartof>Journal of immunological methods, 2011-10, Vol.373 (1-2), p.19-25</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-2b4963ec2a576099e98ffa24504098eba96252764b9df150d46414424472996f3</citedby><cites>FETCH-LOGICAL-c438t-2b4963ec2a576099e98ffa24504098eba96252764b9df150d46414424472996f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jim.2011.07.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24734414$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21821037$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Punyadeera, Chamindie</creatorcontrib><creatorcontrib>Dimeski, Goce</creatorcontrib><creatorcontrib>Kostner, Karam</creatorcontrib><creatorcontrib>Beyerlein, Peter</creatorcontrib><creatorcontrib>Cooper-White, Justin</creatorcontrib><title>One-step homogeneous C-reactive protein assay for saliva</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>Cardiovascular disease is the leading cause of death in the world. Human C-reactive protein (CRP) has been used in the risk assessment of coronary events. Human saliva mirrors the body's health and well-being and is non-invasive, easy to collect and ideal for third world countries as well as for large patient screening. The aim was to establish a saliva CRP reference range and to demonstrate the clinical utility of salivary CRP levels in assessing the coronary events in a primary health care setting.
We have used a homogeneous bead based assay to detect CRP levels in human saliva. We have developed a rapid 15min (vs 90min), sequential, one-step assay to detect CRP in saliva. Saliva was collected from healthy volunteers (n=55, ages 20–70years) as well as from cardiac patients (n=28, ages 43–86years).
The assay incubation time was optimised from 90min to 15min and generated a positive correlation (n=29, range 10–2189pg/mL, r2=0.94; Passing Bablok slope 0.885, Intercept 0, p>0.10), meaning we could decrease the incubation time and produce equivalent results with confidence. The mean CRP level in the saliva of healthy human volunteers was 285pg/mL and in cardiac patients was 1680pg/mL (p<0.01). Analysis of CRP concentrations in paired serum and saliva samples from cardiac patients gave a positive correlation (r2=0.84, p<0.001) and the salivary CRP concentration capable of distinguishing healthy from diseased patients.
The results suggest that this minimally invasive, rapid and sensitive assay will be useful in large patient screening studies for risk assessment of coronary events.
► We have developed 15-min salivary CRP assay compatible for near patient testing. ► Salivary CRP levels are significantly higher in cardiac patients compared to healthy individuals. ► Salivary CRP levels are useful in risk assessment of coronary events.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>blood serum</subject><subject>C-reactive protein</subject><subject>C-Reactive Protein - analysis</subject><subject>cardiovascular diseases</subject><subject>Cardiovascular Diseases - blood</subject><subject>Cardiovascular Diseases - diagnosis</subject><subject>Cardiovascular Diseases - metabolism</subject><subject>Cardiovascular risk factors</subject><subject>death</subject><subject>developing countries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>health services</subject><subject>High-sensitive C-reactive protein assay</subject><subject>Homogeneous bead based assay</subject><subject>Human saliva</subject><subject>Humans</subject><subject>Mass Screening - methods</subject><subject>Middle Aged</subject><subject>Molecular immunology</subject><subject>Non-invasive</subject><subject>patients</subject><subject>Reference Values</subject><subject>Reproducibility of Results</subject><subject>risk assessment</subject><subject>Risk Assessment - methods</subject><subject>Risk Factors</subject><subject>saliva</subject><subject>Saliva - chemistry</subject><subject>screening</subject><subject>Sensitivity and Specificity</subject><subject>Techniques</subject><subject>volunteers</subject><subject>Young Adult</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0ctOGzEUBmCrApVA-wDd0NlUrGY49nh8ESsUQYuElAXN2nI8x-BoLqk9icTb1yFpuysrb75z_Ps3IV8oVBSouF5X69BXDCitQFZA6w9kRpVkpdTQnJAZAGMllY0-I-cprQGAgoCP5IxRxSjUckbUYsAyTbgpXsZ-fMYBx20q5mVE66aww2ITxwnDUNiU7Gvhx1gk24Wd_UROve0Sfj6eF2R5f_dz_qN8XHx_mN8-lo7XairZimtRo2O2kQK0Rq28t4w3wEErXFktWMOk4CvdetpAywWnnDPOJdNa-PqCXB325iC_tpgm04fksOvsW1SjgdWSCxDvSqWV4kxRmSU9SBfHlCJ6s4mht_HVUDD7Zs3a5GbNvlkD0uRm88zlcft21WP7d-JPlRl8OwKbnO18tIML6Z_jsub5adl9PThvR2OfYzbLp3xTk39Hyobtxc1BYO51FzCa5AIODtsQ0U2mHcN_gv4G5NycDg</recordid><startdate>20111028</startdate><enddate>20111028</enddate><creator>Punyadeera, Chamindie</creator><creator>Dimeski, Goce</creator><creator>Kostner, Karam</creator><creator>Beyerlein, Peter</creator><creator>Cooper-White, Justin</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20111028</creationdate><title>One-step homogeneous C-reactive protein assay for saliva</title><author>Punyadeera, Chamindie ; Dimeski, Goce ; Kostner, Karam ; Beyerlein, Peter ; Cooper-White, Justin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-2b4963ec2a576099e98ffa24504098eba96252764b9df150d46414424472996f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>blood serum</topic><topic>C-reactive protein</topic><topic>C-Reactive Protein - analysis</topic><topic>cardiovascular diseases</topic><topic>Cardiovascular Diseases - blood</topic><topic>Cardiovascular Diseases - diagnosis</topic><topic>Cardiovascular Diseases - metabolism</topic><topic>Cardiovascular risk factors</topic><topic>death</topic><topic>developing countries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>health services</topic><topic>High-sensitive C-reactive protein assay</topic><topic>Homogeneous bead based assay</topic><topic>Human saliva</topic><topic>Humans</topic><topic>Mass Screening - methods</topic><topic>Middle Aged</topic><topic>Molecular immunology</topic><topic>Non-invasive</topic><topic>patients</topic><topic>Reference Values</topic><topic>Reproducibility of Results</topic><topic>risk assessment</topic><topic>Risk Assessment - methods</topic><topic>Risk Factors</topic><topic>saliva</topic><topic>Saliva - chemistry</topic><topic>screening</topic><topic>Sensitivity and Specificity</topic><topic>Techniques</topic><topic>volunteers</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Punyadeera, Chamindie</creatorcontrib><creatorcontrib>Dimeski, Goce</creatorcontrib><creatorcontrib>Kostner, Karam</creatorcontrib><creatorcontrib>Beyerlein, Peter</creatorcontrib><creatorcontrib>Cooper-White, Justin</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Punyadeera, Chamindie</au><au>Dimeski, Goce</au><au>Kostner, Karam</au><au>Beyerlein, Peter</au><au>Cooper-White, Justin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>One-step homogeneous C-reactive protein assay for saliva</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>2011-10-28</date><risdate>2011</risdate><volume>373</volume><issue>1-2</issue><spage>19</spage><epage>25</epage><pages>19-25</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>Cardiovascular disease is the leading cause of death in the world. Human C-reactive protein (CRP) has been used in the risk assessment of coronary events. Human saliva mirrors the body's health and well-being and is non-invasive, easy to collect and ideal for third world countries as well as for large patient screening. The aim was to establish a saliva CRP reference range and to demonstrate the clinical utility of salivary CRP levels in assessing the coronary events in a primary health care setting.
We have used a homogeneous bead based assay to detect CRP levels in human saliva. We have developed a rapid 15min (vs 90min), sequential, one-step assay to detect CRP in saliva. Saliva was collected from healthy volunteers (n=55, ages 20–70years) as well as from cardiac patients (n=28, ages 43–86years).
The assay incubation time was optimised from 90min to 15min and generated a positive correlation (n=29, range 10–2189pg/mL, r2=0.94; Passing Bablok slope 0.885, Intercept 0, p>0.10), meaning we could decrease the incubation time and produce equivalent results with confidence. The mean CRP level in the saliva of healthy human volunteers was 285pg/mL and in cardiac patients was 1680pg/mL (p<0.01). Analysis of CRP concentrations in paired serum and saliva samples from cardiac patients gave a positive correlation (r2=0.84, p<0.001) and the salivary CRP concentration capable of distinguishing healthy from diseased patients.
The results suggest that this minimally invasive, rapid and sensitive assay will be useful in large patient screening studies for risk assessment of coronary events.
► We have developed 15-min salivary CRP assay compatible for near patient testing. ► Salivary CRP levels are significantly higher in cardiac patients compared to healthy individuals. ► Salivary CRP levels are useful in risk assessment of coronary events.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21821037</pmid><doi>10.1016/j.jim.2011.07.013</doi><tpages>7</tpages></addata></record> |
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| subjects | Adolescent Adult Aged, 80 and over Biological and medical sciences blood serum C-reactive protein C-Reactive Protein - analysis cardiovascular diseases Cardiovascular Diseases - blood Cardiovascular Diseases - diagnosis Cardiovascular Diseases - metabolism Cardiovascular risk factors death developing countries Fundamental and applied biological sciences. Psychology Fundamental immunology health services High-sensitive C-reactive protein assay Homogeneous bead based assay Human saliva Humans Mass Screening - methods Middle Aged Molecular immunology Non-invasive patients Reference Values Reproducibility of Results risk assessment Risk Assessment - methods Risk Factors saliva Saliva - chemistry screening Sensitivity and Specificity Techniques volunteers Young Adult |
| title | One-step homogeneous C-reactive protein assay for saliva |
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