Splitting and self-assembling of far-red fluorescent protein with an engineered beta strand peptide: Application for alpha-synuclein imaging in mammalian cells

Abstract We introduce the strategic development of self-assembling peptide/protein fragments based on the far-red fluorescent protein mPlum. The first beta strand (mPlum 1, 18 amino acids) of mPlum was engineered to spontaneously bind with the rest of the protein (mPlum 2–11, next 10 beta strands) a...

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Veröffentlicht in:	Biomaterials 2011-12, Vol.32 (34), p.9051-9058
Hauptverfasser:	Keem, Joo Oak, Lee, In Hwan, Kim, Sun Young, Jung, Yongwon, Chung, Bong Hyun
Format:	Artikel
Sprache:	eng
Schlagworte:	Advanced Basic Science alpha-Synuclein - analysis Amino Acid Sequence Amino acids Animals Biosensing Techniques - methods Biosensors Dentistry Escherichia coli - genetics Fluorescent Dyes - chemistry Fluorescent protein HeLa Cells Humans Luminescent Proteins - chemistry Luminescent Proteins - genetics Models, Molecular Molecular evolution Molecular Sequence Data Peptides - chemistry Peptides - genetics Protein Engineering Protein Structure, Secondary Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Red Fluorescent Protein Split protein
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