Protective activity of the Uncaria tomentosa extracts on human erythrocytes in oxidative stress induced by 2,4-dichlorophenol (2,4-DCP) and catechol
Influence of U. tomentosa (250μg/ml) on oxidation of 2′,7′-dichlorodihydrofluoresceine diacetate in human erythrocytes induced by 2,4-DCP (250μg/ml) and catechol (100μg/ml) for 1h of incubation. [Display omitted] ► The extracts from Uncaria tomentosa leaves and bark were not toxic to human erythrocy...
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| Veröffentlicht in: | Food and chemical toxicology 2011-09, Vol.49 (9), p.2202-2211 |
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| creator | Bors, Milena Bukowska, Bożena Pilarski, Radosław Gulewicz, Krzysztof Oszmiański, Jan Michałowicz, Jaromir Koter-Michalak, Maria |
| description | Influence of U. tomentosa (250μg/ml) on oxidation of 2′,7′-dichlorodihydrofluoresceine diacetate in human erythrocytes induced by 2,4-DCP (250μg/ml) and catechol (100μg/ml) for 1h of incubation. [Display omitted]
► The extracts from Uncaria tomentosa leaves and bark were not toxic to human erythrocytes. ► U. tomentosa extracts reveal protective activity against 2,4-dichlorophenol toxicity. ► No protective function of extracts in human erythrocytes incubated with catechol was observed. ► Ethanolic extracts revealed more pronounced ability to inhibit oxidation in human erythrocytes.
The purpose of this study was to evaluate the effect of the ethanolic and aqueous extracts of Uncaria tomentosa on human erythrocytes and additionally the assessment of protective effect of these extracts on hemolysis induction, hemoglobin oxidation, and changes in the level of reactive oxygen species (ROS) and lipid peroxidation, which were provoked by selected xenobiotics, i.e. 2,4-dichlorophenol (2,4-DCP) and catechol.
All tested extracts, even at a very high concentration of 500μg/ml were not toxic to the erythrocytes because they did not cause lipid peroxidation, increase methemoglobin and ROS levels nor provoked hemolysis. The results of this study also revealed protective effect of extracts of U. tomentosa. The extracts studied depleted the extent of hemoglobin oxidation and lipid peroxidation as well as decreased the level of ROS and hemolysis, which was provoked by 2,4-DCP. No protective activity of the extracts against catechol action, which is a precursor of semiquinones in cell was found.
A difference in the effect of the extracts studied was observed. Ethanol-based extracts revealed more pronounced ability to inhibit oxidation processes in human erythrocytes. |
| doi_str_mv | 10.1016/j.fct.2011.06.013 |
| format | Article |
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► The extracts from Uncaria tomentosa leaves and bark were not toxic to human erythrocytes. ► U. tomentosa extracts reveal protective activity against 2,4-dichlorophenol toxicity. ► No protective function of extracts in human erythrocytes incubated with catechol was observed. ► Ethanolic extracts revealed more pronounced ability to inhibit oxidation in human erythrocytes.
The purpose of this study was to evaluate the effect of the ethanolic and aqueous extracts of Uncaria tomentosa on human erythrocytes and additionally the assessment of protective effect of these extracts on hemolysis induction, hemoglobin oxidation, and changes in the level of reactive oxygen species (ROS) and lipid peroxidation, which were provoked by selected xenobiotics, i.e. 2,4-dichlorophenol (2,4-DCP) and catechol.
All tested extracts, even at a very high concentration of 500μg/ml were not toxic to the erythrocytes because they did not cause lipid peroxidation, increase methemoglobin and ROS levels nor provoked hemolysis. The results of this study also revealed protective effect of extracts of U. tomentosa. The extracts studied depleted the extent of hemoglobin oxidation and lipid peroxidation as well as decreased the level of ROS and hemolysis, which was provoked by 2,4-DCP. No protective activity of the extracts against catechol action, which is a precursor of semiquinones in cell was found.
A difference in the effect of the extracts studied was observed. Ethanol-based extracts revealed more pronounced ability to inhibit oxidation processes in human erythrocytes.</description><identifier>ISSN: 0278-6915</identifier><identifier>EISSN: 1873-6351</identifier><identifier>DOI: 10.1016/j.fct.2011.06.013</identifier><identifier>PMID: 21712061</identifier><identifier>CODEN: FCTOD7</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>2,4-dichlorophenol ; Antioxidant activity ; Biological and medical sciences ; Cat's Claw - chemistry ; catechol ; Catechols - pharmacology ; Chlorophenols - toxicity ; Chromatography, High Pressure Liquid ; erythrocytes ; Erythrocytes - drug effects ; Flow Cytometry ; Hemoglobin ; hemolysis ; Human erythrocyte ; Humans ; Lipid peroxidation ; Medical sciences ; oxidation ; Oxidative Stress ; Plant Extracts - pharmacology ; protective effect ; reactive oxygen species ; toxicity ; Toxicology ; Uncaria tomentosa ; xenobiotics</subject><ispartof>Food and chemical toxicology, 2011-09, Vol.49 (9), p.2202-2211</ispartof><rights>2011 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-257ea092d4c2d73e1b231eebaae30288b7533e0e59a42f35d765b9006ec86f163</citedby><cites>FETCH-LOGICAL-c438t-257ea092d4c2d73e1b231eebaae30288b7533e0e59a42f35d765b9006ec86f163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fct.2011.06.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24523751$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21712061$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bors, Milena</creatorcontrib><creatorcontrib>Bukowska, Bożena</creatorcontrib><creatorcontrib>Pilarski, Radosław</creatorcontrib><creatorcontrib>Gulewicz, Krzysztof</creatorcontrib><creatorcontrib>Oszmiański, Jan</creatorcontrib><creatorcontrib>Michałowicz, Jaromir</creatorcontrib><creatorcontrib>Koter-Michalak, Maria</creatorcontrib><title>Protective activity of the Uncaria tomentosa extracts on human erythrocytes in oxidative stress induced by 2,4-dichlorophenol (2,4-DCP) and catechol</title><title>Food and chemical toxicology</title><addtitle>Food Chem Toxicol</addtitle><description>Influence of U. tomentosa (250μg/ml) on oxidation of 2′,7′-dichlorodihydrofluoresceine diacetate in human erythrocytes induced by 2,4-DCP (250μg/ml) and catechol (100μg/ml) for 1h of incubation. [Display omitted]
► The extracts from Uncaria tomentosa leaves and bark were not toxic to human erythrocytes. ► U. tomentosa extracts reveal protective activity against 2,4-dichlorophenol toxicity. ► No protective function of extracts in human erythrocytes incubated with catechol was observed. ► Ethanolic extracts revealed more pronounced ability to inhibit oxidation in human erythrocytes.
The purpose of this study was to evaluate the effect of the ethanolic and aqueous extracts of Uncaria tomentosa on human erythrocytes and additionally the assessment of protective effect of these extracts on hemolysis induction, hemoglobin oxidation, and changes in the level of reactive oxygen species (ROS) and lipid peroxidation, which were provoked by selected xenobiotics, i.e. 2,4-dichlorophenol (2,4-DCP) and catechol.
All tested extracts, even at a very high concentration of 500μg/ml were not toxic to the erythrocytes because they did not cause lipid peroxidation, increase methemoglobin and ROS levels nor provoked hemolysis. The results of this study also revealed protective effect of extracts of U. tomentosa. The extracts studied depleted the extent of hemoglobin oxidation and lipid peroxidation as well as decreased the level of ROS and hemolysis, which was provoked by 2,4-DCP. No protective activity of the extracts against catechol action, which is a precursor of semiquinones in cell was found.
A difference in the effect of the extracts studied was observed. Ethanol-based extracts revealed more pronounced ability to inhibit oxidation processes in human erythrocytes.</description><subject>2,4-dichlorophenol</subject><subject>Antioxidant activity</subject><subject>Biological and medical sciences</subject><subject>Cat's Claw - chemistry</subject><subject>catechol</subject><subject>Catechols - pharmacology</subject><subject>Chlorophenols - toxicity</subject><subject>Chromatography, High Pressure Liquid</subject><subject>erythrocytes</subject><subject>Erythrocytes - drug effects</subject><subject>Flow Cytometry</subject><subject>Hemoglobin</subject><subject>hemolysis</subject><subject>Human erythrocyte</subject><subject>Humans</subject><subject>Lipid peroxidation</subject><subject>Medical sciences</subject><subject>oxidation</subject><subject>Oxidative Stress</subject><subject>Plant Extracts - pharmacology</subject><subject>protective effect</subject><subject>reactive oxygen species</subject><subject>toxicity</subject><subject>Toxicology</subject><subject>Uncaria tomentosa</subject><subject>xenobiotics</subject><issn>0278-6915</issn><issn>1873-6351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhSMEotPCA7ABbxAgkeCf2EnECg2_UiUqwawtx7khHiX21Haq5j36wDidAXasrnT1nXOvzsmyZwQXBBPxbl_0OhYUE1JgUWDCHmQbUlcsF4yTh9kG06rORUP4WXYewh5jXJFKPM7OKKkIxYJssrsr7yLoaG4AqXWYuCDXozgA2lmtvFEouglsdEEhuI0-UQE5i4Z5UhaBX-LgnV4iBGQscremU_duIXoI666bNXSoXRB9W-ad0cPovDsMYN2IXq-7j9urN0jZDmmVXhnc-CR71KsxwNPTvMh2nz_93H7NL79_-bb9cJnrktUxp7wChRvalZp2FQPSUkYAWqWAYVrXbcUZAwy8USXtGe8qwdsGYwG6Fj0R7CJ7dfQ9eHc9Q4hyMkHDOCoLbg6ywZSJpqxZIsmR1N6F4KGXB28m5RdJsFy7kHuZupBrFxILmbpImucn97mdoPur-BN-Al6eABW0GnuvrDbhH1dyyiq-ci-OXK-cVL98YnY_0iWOcbLi907vjwSktG4MeBm0AZtyNz6VKztn_vPobwv2sO8</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Bors, Milena</creator><creator>Bukowska, Bożena</creator><creator>Pilarski, Radosław</creator><creator>Gulewicz, Krzysztof</creator><creator>Oszmiański, Jan</creator><creator>Michałowicz, Jaromir</creator><creator>Koter-Michalak, Maria</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20110901</creationdate><title>Protective activity of the Uncaria tomentosa extracts on human erythrocytes in oxidative stress induced by 2,4-dichlorophenol (2,4-DCP) and catechol</title><author>Bors, Milena ; Bukowska, Bożena ; Pilarski, Radosław ; Gulewicz, Krzysztof ; Oszmiański, Jan ; Michałowicz, Jaromir ; Koter-Michalak, Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-257ea092d4c2d73e1b231eebaae30288b7533e0e59a42f35d765b9006ec86f163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>2,4-dichlorophenol</topic><topic>Antioxidant activity</topic><topic>Biological and medical sciences</topic><topic>Cat's Claw - chemistry</topic><topic>catechol</topic><topic>Catechols - pharmacology</topic><topic>Chlorophenols - toxicity</topic><topic>Chromatography, High Pressure Liquid</topic><topic>erythrocytes</topic><topic>Erythrocytes - drug effects</topic><topic>Flow Cytometry</topic><topic>Hemoglobin</topic><topic>hemolysis</topic><topic>Human erythrocyte</topic><topic>Humans</topic><topic>Lipid peroxidation</topic><topic>Medical sciences</topic><topic>oxidation</topic><topic>Oxidative Stress</topic><topic>Plant Extracts - pharmacology</topic><topic>protective effect</topic><topic>reactive oxygen species</topic><topic>toxicity</topic><topic>Toxicology</topic><topic>Uncaria tomentosa</topic><topic>xenobiotics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bors, Milena</creatorcontrib><creatorcontrib>Bukowska, Bożena</creatorcontrib><creatorcontrib>Pilarski, Radosław</creatorcontrib><creatorcontrib>Gulewicz, Krzysztof</creatorcontrib><creatorcontrib>Oszmiański, Jan</creatorcontrib><creatorcontrib>Michałowicz, Jaromir</creatorcontrib><creatorcontrib>Koter-Michalak, Maria</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Food and chemical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bors, Milena</au><au>Bukowska, Bożena</au><au>Pilarski, Radosław</au><au>Gulewicz, Krzysztof</au><au>Oszmiański, Jan</au><au>Michałowicz, Jaromir</au><au>Koter-Michalak, Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective activity of the Uncaria tomentosa extracts on human erythrocytes in oxidative stress induced by 2,4-dichlorophenol (2,4-DCP) and catechol</atitle><jtitle>Food and chemical toxicology</jtitle><addtitle>Food Chem Toxicol</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>49</volume><issue>9</issue><spage>2202</spage><epage>2211</epage><pages>2202-2211</pages><issn>0278-6915</issn><eissn>1873-6351</eissn><coden>FCTOD7</coden><abstract>Influence of U. tomentosa (250μg/ml) on oxidation of 2′,7′-dichlorodihydrofluoresceine diacetate in human erythrocytes induced by 2,4-DCP (250μg/ml) and catechol (100μg/ml) for 1h of incubation. [Display omitted]
► The extracts from Uncaria tomentosa leaves and bark were not toxic to human erythrocytes. ► U. tomentosa extracts reveal protective activity against 2,4-dichlorophenol toxicity. ► No protective function of extracts in human erythrocytes incubated with catechol was observed. ► Ethanolic extracts revealed more pronounced ability to inhibit oxidation in human erythrocytes.
The purpose of this study was to evaluate the effect of the ethanolic and aqueous extracts of Uncaria tomentosa on human erythrocytes and additionally the assessment of protective effect of these extracts on hemolysis induction, hemoglobin oxidation, and changes in the level of reactive oxygen species (ROS) and lipid peroxidation, which were provoked by selected xenobiotics, i.e. 2,4-dichlorophenol (2,4-DCP) and catechol.
All tested extracts, even at a very high concentration of 500μg/ml were not toxic to the erythrocytes because they did not cause lipid peroxidation, increase methemoglobin and ROS levels nor provoked hemolysis. The results of this study also revealed protective effect of extracts of U. tomentosa. The extracts studied depleted the extent of hemoglobin oxidation and lipid peroxidation as well as decreased the level of ROS and hemolysis, which was provoked by 2,4-DCP. No protective activity of the extracts against catechol action, which is a precursor of semiquinones in cell was found.
A difference in the effect of the extracts studied was observed. Ethanol-based extracts revealed more pronounced ability to inhibit oxidation processes in human erythrocytes.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>21712061</pmid><doi>10.1016/j.fct.2011.06.013</doi><tpages>10</tpages></addata></record> |
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| subjects | 2,4-dichlorophenol Antioxidant activity Biological and medical sciences Cat's Claw - chemistry catechol Catechols - pharmacology Chlorophenols - toxicity Chromatography, High Pressure Liquid erythrocytes Erythrocytes - drug effects Flow Cytometry Hemoglobin hemolysis Human erythrocyte Humans Lipid peroxidation Medical sciences oxidation Oxidative Stress Plant Extracts - pharmacology protective effect reactive oxygen species toxicity Toxicology Uncaria tomentosa xenobiotics |
| title | Protective activity of the Uncaria tomentosa extracts on human erythrocytes in oxidative stress induced by 2,4-dichlorophenol (2,4-DCP) and catechol |
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