Agrobacterium-mediated in planta transformation of maize germ cells
A transfer DNA (T-DNA) carrying the marker gene npt II was detected in the genomes of diploid and haploid maize plants obtained after the treatment of pistil filaments with a suspension of Agrobacterium during artificial pollination. PCR analysis of total DNA isolated from 155 canamycin-resistant di...
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| Veröffentlicht in: | Russian journal of genetics 2010-04, Vol.46 (4), p.501-504 |
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| container_title | Russian journal of genetics |
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| creator | Mamontova, E. M. Velikov, V. A. Volokhina, I. V. Chumakov, M. I. |
| description | A transfer DNA (T-DNA) carrying the marker gene
npt
II was detected in the genomes of diploid and haploid maize plants obtained after the treatment of pistil filaments with a suspension of
Agrobacterium
during artificial pollination. PCR analysis of total DNA isolated from 155 canamycin-resistant diploid F1 seedlings revealed T-DNA insertions in the genomes of 111 plants (32.7% of the total number of analyzed seeds). The example of matroclinal haploids was used to demonstrate that T-DNA may be transported to the egg cell by the growing pollen tube (PT). Twelve out of 16 analyzed haploid plants contained the T-DNA insertion. The possible mechanism of the transfer of the
Agrobacterium
T-DNA to the maize genome during pollination is discussed. |
| doi_str_mv | 10.1134/S1022795410040186 |
| format | Article |
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npt
II was detected in the genomes of diploid and haploid maize plants obtained after the treatment of pistil filaments with a suspension of
Agrobacterium
during artificial pollination. PCR analysis of total DNA isolated from 155 canamycin-resistant diploid F1 seedlings revealed T-DNA insertions in the genomes of 111 plants (32.7% of the total number of analyzed seeds). The example of matroclinal haploids was used to demonstrate that T-DNA may be transported to the egg cell by the growing pollen tube (PT). Twelve out of 16 analyzed haploid plants contained the T-DNA insertion. The possible mechanism of the transfer of the
Agrobacterium
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npt
II was detected in the genomes of diploid and haploid maize plants obtained after the treatment of pistil filaments with a suspension of
Agrobacterium
during artificial pollination. PCR analysis of total DNA isolated from 155 canamycin-resistant diploid F1 seedlings revealed T-DNA insertions in the genomes of 111 plants (32.7% of the total number of analyzed seeds). The example of matroclinal haploids was used to demonstrate that T-DNA may be transported to the egg cell by the growing pollen tube (PT). Twelve out of 16 analyzed haploid plants contained the T-DNA insertion. The possible mechanism of the transfer of the
Agrobacterium
T-DNA to the maize genome during pollination is discussed.</description><subject>Agrobacterium</subject><subject>Animal Genetics and Genomics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Human Genetics</subject><subject>Microbial Genetics and Genomics</subject><subject>Short Communications</subject><subject>Zea mays</subject><issn>1022-7954</issn><issn>1608-3369</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEQhoMoqNUf4C03T6uTj91mj6X4BQUP6jkk2WxJ2SQ1yR7015tSb4KnGWaed5j3ReiGwB0hjN-_EaB02becAHAgojtBF6QD0TDW9ae1r-vmsD9HlznvACrXsQu0Xm1T1MoUm9zsG28Hp4odsAt4P6lQFC5JhTzG5FVxMeA4Yq_ct8Vbmzw2dpryFTob1ZTt9W9doI_Hh_f1c7N5fXpZrzaNYRRKY1rO-VK3zHKqejEyK7Qhum_VCIaDrbOBaiqoHgQM0OqBmSXQXrO204wItkC3x7v7FD9nm4v0Lh8-UMHGOcseKOsEA1ZJciRNijknO8p9cl6lL0lAHvKSf_KqGnrU5MqG6k7u4pxCNfSP6AdD_2wv</recordid><startdate>20100401</startdate><enddate>20100401</enddate><creator>Mamontova, E. M.</creator><creator>Velikov, V. A.</creator><creator>Volokhina, I. V.</creator><creator>Chumakov, M. I.</creator><general>SP MAIK Nauka/Interperiodica</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20100401</creationdate><title>Agrobacterium-mediated in planta transformation of maize germ cells</title><author>Mamontova, E. M. ; Velikov, V. A. ; Volokhina, I. V. ; Chumakov, M. I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c320t-c54447b53e42a98f3e8bc1b95af0c40ea98d2b282bd80d05bd3c7029b356b3183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Agrobacterium</topic><topic>Animal Genetics and Genomics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Human Genetics</topic><topic>Microbial Genetics and Genomics</topic><topic>Short Communications</topic><topic>Zea mays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mamontova, E. M.</creatorcontrib><creatorcontrib>Velikov, V. A.</creatorcontrib><creatorcontrib>Volokhina, I. V.</creatorcontrib><creatorcontrib>Chumakov, M. I.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Russian journal of genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mamontova, E. M.</au><au>Velikov, V. A.</au><au>Volokhina, I. V.</au><au>Chumakov, M. I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Agrobacterium-mediated in planta transformation of maize germ cells</atitle><jtitle>Russian journal of genetics</jtitle><stitle>Russ J Genet</stitle><date>2010-04-01</date><risdate>2010</risdate><volume>46</volume><issue>4</issue><spage>501</spage><epage>504</epage><pages>501-504</pages><issn>1022-7954</issn><eissn>1608-3369</eissn><abstract>A transfer DNA (T-DNA) carrying the marker gene
npt
II was detected in the genomes of diploid and haploid maize plants obtained after the treatment of pistil filaments with a suspension of
Agrobacterium
during artificial pollination. PCR analysis of total DNA isolated from 155 canamycin-resistant diploid F1 seedlings revealed T-DNA insertions in the genomes of 111 plants (32.7% of the total number of analyzed seeds). The example of matroclinal haploids was used to demonstrate that T-DNA may be transported to the egg cell by the growing pollen tube (PT). Twelve out of 16 analyzed haploid plants contained the T-DNA insertion. The possible mechanism of the transfer of the
Agrobacterium
T-DNA to the maize genome during pollination is discussed.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><doi>10.1134/S1022795410040186</doi><tpages>4</tpages></addata></record> |
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| ispartof | Russian journal of genetics, 2010-04, Vol.46 (4), p.501-504 |
| issn | 1022-7954 1608-3369 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Agrobacterium Animal Genetics and Genomics Biomedical and Life Sciences Biomedicine Human Genetics Microbial Genetics and Genomics Short Communications Zea mays |
| title | Agrobacterium-mediated in planta transformation of maize germ cells |
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