Evaluation of sub-lethal effects of endosulfan on cortisol secretion, glutathione S-transferase and acetylcholinesterase activities in Clarias gariepinus
The effects of endosulfan, a widely used organochlorine pesticide in the Niger-Delta ecological zone were examined on cortisol secretion, glutathione S-transferase and acetylcholinesterase enzymatic activities in the serum of a dominant fresh water fish species, Clarias gariepinus. Juveniles stage o...
Gespeichert in:
| Veröffentlicht in: | Food and chemical toxicology 2011-09, Vol.49 (9), p.1898-1903 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1903 |
|---|---|
| container_issue | 9 |
| container_start_page | 1898 |
| container_title | Food and chemical toxicology |
| container_volume | 49 |
| creator | Ezemonye, L.I.N. Ikpesu, T.O. |
| description | The effects of endosulfan, a widely used organochlorine pesticide in the Niger-Delta ecological zone were examined on cortisol secretion, glutathione S-transferase and acetylcholinesterase enzymatic activities in the serum of a dominant fresh water fish species, Clarias gariepinus. Juveniles stage of C. gariepinus were used for the toxicity study. Lethal concentration of 50% mortality of sample (LC50) was determined using semi-static method. From the result of the LC50 determination lower concentrations of endosulfan were prepared for sub-lethal test. Ten fish per test concentration in three replicates were exposed to varying concentrations of endosulfan (0.00, 0.0025, 0.005, 0.0075 and 0.01) μg/L. Signs of stress and mortality were monitored during the experimental duration. Cortisol levels, GST and AChE activities were measured spectrophotometrically. No visible changes in behaviour and appearance were observed in the treated fishes and no mortality recorded. Significant (p |
| doi_str_mv | 10.1016/j.fct.2010.10.025 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_902366971</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0278691510006447</els_id><sourcerecordid>902366971</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-b1b98e60fa39517745038d15b9ca7b1de60d575be77755abbe4bed103d1b1b3b3</originalsourceid><addsrcrecordid>eNp9kU2PEzEMhkcIxJaFH8AFckFcmJJMmsmMOKFq-ZBW4rDsOcqH06ZKJyXOVNqfwr8lpQVunJzYz2tbfpvmJaNLRln_frf0tiw7-vu_pJ141CzYIHnbc8EeNwvayaHtRyaummeIO0qpZLJ_2lx1jIq-Z2zR_Lw56jjrEtJEkic4mzZC2epIwHuwBU9ZmFzCOXpdmYnYlEvAFAmCzXBSviObOBddtvUN5K4tWU_oIWsEoidHtIXyEO02xTABlkvBlnAMJQCSMJF11DloJJsa4BCmGZ83T7yOCC8u8bq5_3Tzff2lvf32-ev6421rV3worWFmHKCnXvNRMClXgvLBMWFGq6VhrpackMKAlFIIbQysDDhGuWNVyg2_bt6e-x5y-jHX9dQ-oIUY9QRpRjXSjvf9KFkl2Zm0OSFm8OqQw17nB8WoOhmidqoaok6GnFLVkKp5dek-mz24v4o_DlTgzQXQaHX09XQ24D9uJTpeZ1fu9ZnzOim9yZW5v6uTBKVMMsGHSnw4E1CvdQyQFdoAkwUXcnVSuRT-s-gvLea1vw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>902366971</pqid></control><display><type>article</type><title>Evaluation of sub-lethal effects of endosulfan on cortisol secretion, glutathione S-transferase and acetylcholinesterase activities in Clarias gariepinus</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Ezemonye, L.I.N. ; Ikpesu, T.O.</creator><creatorcontrib>Ezemonye, L.I.N. ; Ikpesu, T.O.</creatorcontrib><description>The effects of endosulfan, a widely used organochlorine pesticide in the Niger-Delta ecological zone were examined on cortisol secretion, glutathione S-transferase and acetylcholinesterase enzymatic activities in the serum of a dominant fresh water fish species, Clarias gariepinus. Juveniles stage of C. gariepinus were used for the toxicity study. Lethal concentration of 50% mortality of sample (LC50) was determined using semi-static method. From the result of the LC50 determination lower concentrations of endosulfan were prepared for sub-lethal test. Ten fish per test concentration in three replicates were exposed to varying concentrations of endosulfan (0.00, 0.0025, 0.005, 0.0075 and 0.01) μg/L. Signs of stress and mortality were monitored during the experimental duration. Cortisol levels, GST and AChE activities were measured spectrophotometrically. No visible changes in behaviour and appearance were observed in the treated fishes and no mortality recorded. Significant (p<0.05) declined in cortisol secretion was observed and the change in the concentration was dose and time dependent. Glutathione S-transferase activity was significantly (p<0.05) higher than the control and the concentrations increase with increased in concentration and exposure duration. No clear trend was observed in acetylcholinesterase activity. The findings showed that the cortisol levels and GST activity of test organism were affected by the test chemical and therefore could be considered as a suitable marker to evaluate the effect of organochlorine pesticide on fish and other aquatic organisms.</description><identifier>ISSN: 0278-6915</identifier><identifier>EISSN: 1873-6351</identifier><identifier>DOI: 10.1016/j.fct.2010.10.025</identifier><identifier>PMID: 21056611</identifier><identifier>CODEN: FCTOD7</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Acetylcholinesterase ; Acetylcholinesterase - blood ; Animals ; behavior change ; Biological and medical sciences ; blood serum ; Clarias gariepinus ; Cortisol ; Endosulfan ; Endosulfan - toxicity ; enzyme activity ; exposure duration ; Fish ; Fishes - metabolism ; freshwater fish ; Glutathione ; Glutathione S-transferase ; glutathione transferase ; Glutathione Transferase - blood ; Hydrocortisone - blood ; Hydrocortisone - secretion ; juveniles ; lethal concentration 50 ; Medical sciences ; Mortality ; Niger-Delta ; Organisms ; Pesticides ; Pesticides - toxicity ; Pesticides, fertilizers and other agrochemicals toxicology ; secretion ; Secretions ; Stress concentration ; sublethal effects ; Toxicity ; Toxicology</subject><ispartof>Food and chemical toxicology, 2011-09, Vol.49 (9), p.1898-1903</ispartof><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-b1b98e60fa39517745038d15b9ca7b1de60d575be77755abbe4bed103d1b1b3b3</citedby><cites>FETCH-LOGICAL-c438t-b1b98e60fa39517745038d15b9ca7b1de60d575be77755abbe4bed103d1b1b3b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fct.2010.10.025$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24523713$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21056611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ezemonye, L.I.N.</creatorcontrib><creatorcontrib>Ikpesu, T.O.</creatorcontrib><title>Evaluation of sub-lethal effects of endosulfan on cortisol secretion, glutathione S-transferase and acetylcholinesterase activities in Clarias gariepinus</title><title>Food and chemical toxicology</title><addtitle>Food Chem Toxicol</addtitle><description>The effects of endosulfan, a widely used organochlorine pesticide in the Niger-Delta ecological zone were examined on cortisol secretion, glutathione S-transferase and acetylcholinesterase enzymatic activities in the serum of a dominant fresh water fish species, Clarias gariepinus. Juveniles stage of C. gariepinus were used for the toxicity study. Lethal concentration of 50% mortality of sample (LC50) was determined using semi-static method. From the result of the LC50 determination lower concentrations of endosulfan were prepared for sub-lethal test. Ten fish per test concentration in three replicates were exposed to varying concentrations of endosulfan (0.00, 0.0025, 0.005, 0.0075 and 0.01) μg/L. Signs of stress and mortality were monitored during the experimental duration. Cortisol levels, GST and AChE activities were measured spectrophotometrically. No visible changes in behaviour and appearance were observed in the treated fishes and no mortality recorded. Significant (p<0.05) declined in cortisol secretion was observed and the change in the concentration was dose and time dependent. Glutathione S-transferase activity was significantly (p<0.05) higher than the control and the concentrations increase with increased in concentration and exposure duration. No clear trend was observed in acetylcholinesterase activity. The findings showed that the cortisol levels and GST activity of test organism were affected by the test chemical and therefore could be considered as a suitable marker to evaluate the effect of organochlorine pesticide on fish and other aquatic organisms.</description><subject>Acetylcholinesterase</subject><subject>Acetylcholinesterase - blood</subject><subject>Animals</subject><subject>behavior change</subject><subject>Biological and medical sciences</subject><subject>blood serum</subject><subject>Clarias gariepinus</subject><subject>Cortisol</subject><subject>Endosulfan</subject><subject>Endosulfan - toxicity</subject><subject>enzyme activity</subject><subject>exposure duration</subject><subject>Fish</subject><subject>Fishes - metabolism</subject><subject>freshwater fish</subject><subject>Glutathione</subject><subject>Glutathione S-transferase</subject><subject>glutathione transferase</subject><subject>Glutathione Transferase - blood</subject><subject>Hydrocortisone - blood</subject><subject>Hydrocortisone - secretion</subject><subject>juveniles</subject><subject>lethal concentration 50</subject><subject>Medical sciences</subject><subject>Mortality</subject><subject>Niger-Delta</subject><subject>Organisms</subject><subject>Pesticides</subject><subject>Pesticides - toxicity</subject><subject>Pesticides, fertilizers and other agrochemicals toxicology</subject><subject>secretion</subject><subject>Secretions</subject><subject>Stress concentration</subject><subject>sublethal effects</subject><subject>Toxicity</subject><subject>Toxicology</subject><issn>0278-6915</issn><issn>1873-6351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2PEzEMhkcIxJaFH8AFckFcmJJMmsmMOKFq-ZBW4rDsOcqH06ZKJyXOVNqfwr8lpQVunJzYz2tbfpvmJaNLRln_frf0tiw7-vu_pJ141CzYIHnbc8EeNwvayaHtRyaummeIO0qpZLJ_2lx1jIq-Z2zR_Lw56jjrEtJEkic4mzZC2epIwHuwBU9ZmFzCOXpdmYnYlEvAFAmCzXBSviObOBddtvUN5K4tWU_oIWsEoidHtIXyEO02xTABlkvBlnAMJQCSMJF11DloJJsa4BCmGZ83T7yOCC8u8bq5_3Tzff2lvf32-ev6421rV3worWFmHKCnXvNRMClXgvLBMWFGq6VhrpackMKAlFIIbQysDDhGuWNVyg2_bt6e-x5y-jHX9dQ-oIUY9QRpRjXSjvf9KFkl2Zm0OSFm8OqQw17nB8WoOhmidqoaok6GnFLVkKp5dek-mz24v4o_DlTgzQXQaHX09XQ24D9uJTpeZ1fu9ZnzOim9yZW5v6uTBKVMMsGHSnw4E1CvdQyQFdoAkwUXcnVSuRT-s-gvLea1vw</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Ezemonye, L.I.N.</creator><creator>Ikpesu, T.O.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>20110901</creationdate><title>Evaluation of sub-lethal effects of endosulfan on cortisol secretion, glutathione S-transferase and acetylcholinesterase activities in Clarias gariepinus</title><author>Ezemonye, L.I.N. ; Ikpesu, T.O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-b1b98e60fa39517745038d15b9ca7b1de60d575be77755abbe4bed103d1b1b3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Acetylcholinesterase</topic><topic>Acetylcholinesterase - blood</topic><topic>Animals</topic><topic>behavior change</topic><topic>Biological and medical sciences</topic><topic>blood serum</topic><topic>Clarias gariepinus</topic><topic>Cortisol</topic><topic>Endosulfan</topic><topic>Endosulfan - toxicity</topic><topic>enzyme activity</topic><topic>exposure duration</topic><topic>Fish</topic><topic>Fishes - metabolism</topic><topic>freshwater fish</topic><topic>Glutathione</topic><topic>Glutathione S-transferase</topic><topic>glutathione transferase</topic><topic>Glutathione Transferase - blood</topic><topic>Hydrocortisone - blood</topic><topic>Hydrocortisone - secretion</topic><topic>juveniles</topic><topic>lethal concentration 50</topic><topic>Medical sciences</topic><topic>Mortality</topic><topic>Niger-Delta</topic><topic>Organisms</topic><topic>Pesticides</topic><topic>Pesticides - toxicity</topic><topic>Pesticides, fertilizers and other agrochemicals toxicology</topic><topic>secretion</topic><topic>Secretions</topic><topic>Stress concentration</topic><topic>sublethal effects</topic><topic>Toxicity</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ezemonye, L.I.N.</creatorcontrib><creatorcontrib>Ikpesu, T.O.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Food and chemical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ezemonye, L.I.N.</au><au>Ikpesu, T.O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of sub-lethal effects of endosulfan on cortisol secretion, glutathione S-transferase and acetylcholinesterase activities in Clarias gariepinus</atitle><jtitle>Food and chemical toxicology</jtitle><addtitle>Food Chem Toxicol</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>49</volume><issue>9</issue><spage>1898</spage><epage>1903</epage><pages>1898-1903</pages><issn>0278-6915</issn><eissn>1873-6351</eissn><coden>FCTOD7</coden><abstract>The effects of endosulfan, a widely used organochlorine pesticide in the Niger-Delta ecological zone were examined on cortisol secretion, glutathione S-transferase and acetylcholinesterase enzymatic activities in the serum of a dominant fresh water fish species, Clarias gariepinus. Juveniles stage of C. gariepinus were used for the toxicity study. Lethal concentration of 50% mortality of sample (LC50) was determined using semi-static method. From the result of the LC50 determination lower concentrations of endosulfan were prepared for sub-lethal test. Ten fish per test concentration in three replicates were exposed to varying concentrations of endosulfan (0.00, 0.0025, 0.005, 0.0075 and 0.01) μg/L. Signs of stress and mortality were monitored during the experimental duration. Cortisol levels, GST and AChE activities were measured spectrophotometrically. No visible changes in behaviour and appearance were observed in the treated fishes and no mortality recorded. Significant (p<0.05) declined in cortisol secretion was observed and the change in the concentration was dose and time dependent. Glutathione S-transferase activity was significantly (p<0.05) higher than the control and the concentrations increase with increased in concentration and exposure duration. No clear trend was observed in acetylcholinesterase activity. The findings showed that the cortisol levels and GST activity of test organism were affected by the test chemical and therefore could be considered as a suitable marker to evaluate the effect of organochlorine pesticide on fish and other aquatic organisms.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>21056611</pmid><doi>10.1016/j.fct.2010.10.025</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0278-6915 |
| ispartof | Food and chemical toxicology, 2011-09, Vol.49 (9), p.1898-1903 |
| issn | 0278-6915 1873-6351 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_902366971 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acetylcholinesterase Acetylcholinesterase - blood Animals behavior change Biological and medical sciences blood serum Clarias gariepinus Cortisol Endosulfan Endosulfan - toxicity enzyme activity exposure duration Fish Fishes - metabolism freshwater fish Glutathione Glutathione S-transferase glutathione transferase Glutathione Transferase - blood Hydrocortisone - blood Hydrocortisone - secretion juveniles lethal concentration 50 Medical sciences Mortality Niger-Delta Organisms Pesticides Pesticides - toxicity Pesticides, fertilizers and other agrochemicals toxicology secretion Secretions Stress concentration sublethal effects Toxicity Toxicology |
| title | Evaluation of sub-lethal effects of endosulfan on cortisol secretion, glutathione S-transferase and acetylcholinesterase activities in Clarias gariepinus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T02%3A30%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20sub-lethal%20effects%20of%20endosulfan%20on%20cortisol%20secretion,%20glutathione%20S-transferase%20and%20acetylcholinesterase%20activities%20in%20Clarias%20gariepinus&rft.jtitle=Food%20and%20chemical%20toxicology&rft.au=Ezemonye,%20L.I.N.&rft.date=2011-09-01&rft.volume=49&rft.issue=9&rft.spage=1898&rft.epage=1903&rft.pages=1898-1903&rft.issn=0278-6915&rft.eissn=1873-6351&rft.coden=FCTOD7&rft_id=info:doi/10.1016/j.fct.2010.10.025&rft_dat=%3Cproquest_cross%3E902366971%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=902366971&rft_id=info:pmid/21056611&rft_els_id=S0278691510006447&rfr_iscdi=true |