Identification and characterization of Apple stem grooving virus causing leaf distortion on pear (Pyrus pyrifolia) in Taiwan
A putative virus-induced disease of pear (Pyrus pyrifolia var. Hengshen) showing symptoms of reduced size of foliage and leaf distortion was observed in orchards in central Taiwan in 2004. The sap of symptomatic leaf samples reacted positively to an antiserum against Apple stem grooving virus (ASGV)...
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| Veröffentlicht in: | European journal of plant pathology 2010-09, Vol.128 (1), p.71-79 |
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| creator | Wu, Zhong-Bin Zheng, You-Xiu Su, Chiou-Chu Chang, Chung-Jan Jan, Fuh-Jyh |
| description | A putative virus-induced disease of pear (Pyrus pyrifolia var. Hengshen) showing symptoms of reduced size of foliage and leaf distortion was observed in orchards in central Taiwan in 2004. The sap of symptomatic leaf samples reacted positively to an antiserum against Apple stem grooving virus (ASGV). Two virus cultures, designated as TS1 and TS2, were isolated from symptomatic pears. Flexuous filamentous virions of ∼ 12 × 600 nm were observed in symptomatic pear leaves and purified virus preparations. Results of back inoculation of pear seedlings with TS1 revealed that ASGV was the causal agent of the disease. Sequence analyses of the cloned coat protein (CP) genes of TS1 and TS2 shared 88-92.4% nucleotide and 90.7-97.1% amino acid identities with those of other ASGV isolates available in GenBank. The polyclonal antibody generated against ASGV TS1 has been routinely used for the detection of the ASGV-infection in the imported pear scions for quarantine purpose via enzyme-linked immunosorbent assays (ELISAs). One of 1,199 samples of pear scions imported from Japan during 2005-2007 was identified as ASGV-positive and the virus was designated as AGJP-22. The CP gene amplified from this AGJP-22 shared 97.9-98.3% amino acid identities to those of the domestic isolates and they were closely related phylogenetically. To date, these data present for the first time conclusive evidence revealing that ASGV is indeed the causal agent of the pear disease displaying symptoms of reduced size of foliage and leaf distortion in Taiwan. |
| doi_str_mv | 10.1007/s10658-010-9631-z |
| format | Article |
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Hengshen) showing symptoms of reduced size of foliage and leaf distortion was observed in orchards in central Taiwan in 2004. The sap of symptomatic leaf samples reacted positively to an antiserum against Apple stem grooving virus (ASGV). Two virus cultures, designated as TS1 and TS2, were isolated from symptomatic pears. Flexuous filamentous virions of ∼ 12 × 600 nm were observed in symptomatic pear leaves and purified virus preparations. Results of back inoculation of pear seedlings with TS1 revealed that ASGV was the causal agent of the disease. Sequence analyses of the cloned coat protein (CP) genes of TS1 and TS2 shared 88-92.4% nucleotide and 90.7-97.1% amino acid identities with those of other ASGV isolates available in GenBank. The polyclonal antibody generated against ASGV TS1 has been routinely used for the detection of the ASGV-infection in the imported pear scions for quarantine purpose via enzyme-linked immunosorbent assays (ELISAs). One of 1,199 samples of pear scions imported from Japan during 2005-2007 was identified as ASGV-positive and the virus was designated as AGJP-22. The CP gene amplified from this AGJP-22 shared 97.9-98.3% amino acid identities to those of the domestic isolates and they were closely related phylogenetically. To date, these data present for the first time conclusive evidence revealing that ASGV is indeed the causal agent of the pear disease displaying symptoms of reduced size of foliage and leaf distortion in Taiwan.</description><identifier>ISSN: 0929-1873</identifier><identifier>EISSN: 1573-8469</identifier><identifier>DOI: 10.1007/s10658-010-9631-z</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Agriculture ; Amino acid sequence ; Amino acids ; Antibodies ; Apple stem grooving virus ; ASGV ; Biological and medical sciences ; Biomedical and Life Sciences ; Capillovirus ; Coat protein ; CP gene ; Distortion ; Ecology ; Foliage ; Fruits ; Fundamental and applied biological sciences. Psychology ; Grooving ; Immunoassays ; Inoculation ; Leaves ; Life Sciences ; Nucleotides ; Orchards ; Pear disease ; Pears ; Phylogenetic analysis ; Phylogenetics ; Phylogeny ; Phytopathology. Animal pests. Plant and forest protection ; Plant diseases ; Plant Pathology ; Plant Sciences ; Plant viruses and viroids ; Polyclonal antibodies ; Pome fruit ; Pyrus pyrifolia ; Rosaceae ; Scions ; Seedlings ; Signs and symptoms ; Size reduction ; Stems ; Trees ; Virions ; Viruses</subject><ispartof>European journal of plant pathology, 2010-09, Vol.128 (1), p.71-79</ispartof><rights>KNPV 2010</rights><rights>2015 INIST-CNRS</rights><rights>KNPV 2010.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-8dd2f957be4a4a93af0055adaabd6983fd0a47beec2f2ea3ef210d58cb4b90e33</citedby><cites>FETCH-LOGICAL-c429t-8dd2f957be4a4a93af0055adaabd6983fd0a47beec2f2ea3ef210d58cb4b90e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10658-010-9631-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10658-010-9631-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23087872$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Zhong-Bin</creatorcontrib><creatorcontrib>Zheng, You-Xiu</creatorcontrib><creatorcontrib>Su, Chiou-Chu</creatorcontrib><creatorcontrib>Chang, Chung-Jan</creatorcontrib><creatorcontrib>Jan, Fuh-Jyh</creatorcontrib><title>Identification and characterization of Apple stem grooving virus causing leaf distortion on pear (Pyrus pyrifolia) in Taiwan</title><title>European journal of plant pathology</title><addtitle>Eur J Plant Pathol</addtitle><description>A putative virus-induced disease of pear (Pyrus pyrifolia var. Hengshen) showing symptoms of reduced size of foliage and leaf distortion was observed in orchards in central Taiwan in 2004. The sap of symptomatic leaf samples reacted positively to an antiserum against Apple stem grooving virus (ASGV). Two virus cultures, designated as TS1 and TS2, were isolated from symptomatic pears. Flexuous filamentous virions of ∼ 12 × 600 nm were observed in symptomatic pear leaves and purified virus preparations. Results of back inoculation of pear seedlings with TS1 revealed that ASGV was the causal agent of the disease. Sequence analyses of the cloned coat protein (CP) genes of TS1 and TS2 shared 88-92.4% nucleotide and 90.7-97.1% amino acid identities with those of other ASGV isolates available in GenBank. The polyclonal antibody generated against ASGV TS1 has been routinely used for the detection of the ASGV-infection in the imported pear scions for quarantine purpose via enzyme-linked immunosorbent assays (ELISAs). One of 1,199 samples of pear scions imported from Japan during 2005-2007 was identified as ASGV-positive and the virus was designated as AGJP-22. The CP gene amplified from this AGJP-22 shared 97.9-98.3% amino acid identities to those of the domestic isolates and they were closely related phylogenetically. To date, these data present for the first time conclusive evidence revealing that ASGV is indeed the causal agent of the pear disease displaying symptoms of reduced size of foliage and leaf distortion in Taiwan.</description><subject>Agriculture</subject><subject>Amino acid sequence</subject><subject>Amino acids</subject><subject>Antibodies</subject><subject>Apple stem grooving virus</subject><subject>ASGV</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Capillovirus</subject><subject>Coat protein</subject><subject>CP gene</subject><subject>Distortion</subject><subject>Ecology</subject><subject>Foliage</subject><subject>Fruits</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Grooving</subject><subject>Immunoassays</subject><subject>Inoculation</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Nucleotides</subject><subject>Orchards</subject><subject>Pear disease</subject><subject>Pears</subject><subject>Phylogenetic analysis</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant diseases</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Plant viruses and viroids</subject><subject>Polyclonal antibodies</subject><subject>Pome fruit</subject><subject>Pyrus pyrifolia</subject><subject>Rosaceae</subject><subject>Scions</subject><subject>Seedlings</subject><subject>Signs and symptoms</subject><subject>Size reduction</subject><subject>Stems</subject><subject>Trees</subject><subject>Virions</subject><subject>Viruses</subject><issn>0929-1873</issn><issn>1573-8469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kt1rFDEUxQdRcK3-AT4ZFKk-jN4k85E8lmK1UFCwfQ5387GmzCZjMlPZxT_eDFMUBH0Kyf2dw7mcVNVzCu8oQP8-U-haUQOFWnac1scH1Ya2Pa9F08mH1QYkkzUVPX9cPcn5FopGSrapfl4aGybvvMbJx0AwGKK_YUI92eSP62N05GwcB0vyZPdkl2K882FH7nyaM9E45-U2WHTE-DzFtIoCGS0m8ubLYcHGQ_IuDh7fEh_INfofGJ5WjxwO2T67P0-qm4sP1-ef6qvPHy_Pz65q3TA51cIY5mTbb22DDUqODqBt0SBuTScFdwawKVOrmWMWuXWMgmmF3jZbCZbzk-p09R1T_D7bPKm9z9oOAwYb56wkMN61TScK-fIv8jbOKZRwqm9oI0HyBXr1L4h1JScF2tJC0ZXSKeacrFNj8ntMB0VBLZ2ptTNVOlNLZ-pYNK_vnTFrHFzCoH3-LWQcRC96Vji2crmMws6mPwn-Z_5iFTmMCnepGN98ZUA5LP-Cl-1_AXBesjk</recordid><startdate>20100901</startdate><enddate>20100901</enddate><creator>Wu, Zhong-Bin</creator><creator>Zheng, You-Xiu</creator><creator>Su, Chiou-Chu</creator><creator>Chang, Chung-Jan</creator><creator>Jan, Fuh-Jyh</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X2</scope><scope>88A</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20100901</creationdate><title>Identification and characterization of Apple stem grooving virus causing leaf distortion on pear (Pyrus pyrifolia) in Taiwan</title><author>Wu, Zhong-Bin ; Zheng, You-Xiu ; Su, Chiou-Chu ; Chang, Chung-Jan ; Jan, Fuh-Jyh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-8dd2f957be4a4a93af0055adaabd6983fd0a47beec2f2ea3ef210d58cb4b90e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Agriculture</topic><topic>Amino acid sequence</topic><topic>Amino acids</topic><topic>Antibodies</topic><topic>Apple stem grooving virus</topic><topic>ASGV</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Capillovirus</topic><topic>Coat protein</topic><topic>CP gene</topic><topic>Distortion</topic><topic>Ecology</topic><topic>Foliage</topic><topic>Fruits</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Grooving</topic><topic>Immunoassays</topic><topic>Inoculation</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Nucleotides</topic><topic>Orchards</topic><topic>Pear disease</topic><topic>Pears</topic><topic>Phylogenetic analysis</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant diseases</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Plant viruses and viroids</topic><topic>Polyclonal antibodies</topic><topic>Pome fruit</topic><topic>Pyrus pyrifolia</topic><topic>Rosaceae</topic><topic>Scions</topic><topic>Seedlings</topic><topic>Signs and symptoms</topic><topic>Size reduction</topic><topic>Stems</topic><topic>Trees</topic><topic>Virions</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Zhong-Bin</creatorcontrib><creatorcontrib>Zheng, You-Xiu</creatorcontrib><creatorcontrib>Su, Chiou-Chu</creatorcontrib><creatorcontrib>Chang, Chung-Jan</creatorcontrib><creatorcontrib>Jan, Fuh-Jyh</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Agriculture & Environmental Science Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>European journal of plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Zhong-Bin</au><au>Zheng, You-Xiu</au><au>Su, Chiou-Chu</au><au>Chang, Chung-Jan</au><au>Jan, Fuh-Jyh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of Apple stem grooving virus causing leaf distortion on pear (Pyrus pyrifolia) in Taiwan</atitle><jtitle>European journal of plant pathology</jtitle><stitle>Eur J Plant Pathol</stitle><date>2010-09-01</date><risdate>2010</risdate><volume>128</volume><issue>1</issue><spage>71</spage><epage>79</epage><pages>71-79</pages><issn>0929-1873</issn><eissn>1573-8469</eissn><abstract>A putative virus-induced disease of pear (Pyrus pyrifolia var. Hengshen) showing symptoms of reduced size of foliage and leaf distortion was observed in orchards in central Taiwan in 2004. The sap of symptomatic leaf samples reacted positively to an antiserum against Apple stem grooving virus (ASGV). Two virus cultures, designated as TS1 and TS2, were isolated from symptomatic pears. Flexuous filamentous virions of ∼ 12 × 600 nm were observed in symptomatic pear leaves and purified virus preparations. Results of back inoculation of pear seedlings with TS1 revealed that ASGV was the causal agent of the disease. Sequence analyses of the cloned coat protein (CP) genes of TS1 and TS2 shared 88-92.4% nucleotide and 90.7-97.1% amino acid identities with those of other ASGV isolates available in GenBank. The polyclonal antibody generated against ASGV TS1 has been routinely used for the detection of the ASGV-infection in the imported pear scions for quarantine purpose via enzyme-linked immunosorbent assays (ELISAs). One of 1,199 samples of pear scions imported from Japan during 2005-2007 was identified as ASGV-positive and the virus was designated as AGJP-22. The CP gene amplified from this AGJP-22 shared 97.9-98.3% amino acid identities to those of the domestic isolates and they were closely related phylogenetically. To date, these data present for the first time conclusive evidence revealing that ASGV is indeed the causal agent of the pear disease displaying symptoms of reduced size of foliage and leaf distortion in Taiwan.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><doi>10.1007/s10658-010-9631-z</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Agriculture Amino acid sequence Amino acids Antibodies Apple stem grooving virus ASGV Biological and medical sciences Biomedical and Life Sciences Capillovirus Coat protein CP gene Distortion Ecology Foliage Fruits Fundamental and applied biological sciences. Psychology Grooving Immunoassays Inoculation Leaves Life Sciences Nucleotides Orchards Pear disease Pears Phylogenetic analysis Phylogenetics Phylogeny Phytopathology. Animal pests. Plant and forest protection Plant diseases Plant Pathology Plant Sciences Plant viruses and viroids Polyclonal antibodies Pome fruit Pyrus pyrifolia Rosaceae Scions Seedlings Signs and symptoms Size reduction Stems Trees Virions Viruses |
| title | Identification and characterization of Apple stem grooving virus causing leaf distortion on pear (Pyrus pyrifolia) in Taiwan |
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