Callus formation, plant regeneration, and transient expression in the halophyte sea aster (Aster tripolium L.)
An in vitro regeneration and transient expression systems were developed for the halophyte sea aster (Aster tripolium L.), an important genetic resource for salt tolerance. Adventitious shoots were formed from both leaf explants and suspension-cultured cells in a Murashige and Skoog (MS) (Physiol Pl...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2009-09, Vol.98 (3), p.303-309 |
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| creator | Uno, Yuichi Nakao, Shigeyuki Yamai, Yumiko Koyama, Ryohei Kanechi, Michio Inagaki, Noboru |
| description | An in vitro regeneration and transient expression systems were developed for the halophyte sea aster (Aster tripolium L.), an important genetic resource for salt tolerance. Adventitious shoots were formed from both leaf explants and suspension-cultured cells in a Murashige and Skoog (MS) (Physiol Plant 15:473-497, 1962) basal salts containing 500 mg l⁻¹ casamino acids, and supplemented with 5.4 μM a-naphthaleneacetic acid (NAA) and 4.7 μM kinetin to the culture medium. Hyperhydricity of shoots was avoided by increasing the ventilation of the culture vessel. Root formation from shoots was promoted in the presence of 26.9 μM NAA. A high yield of protoplasts was isolated using 1% cellulase and 0.25% pectinase from both leaf mesophyll and suspension-cultured cells, and these were used for transient expression. The highest level of transient expression of the green fluorescent protein was obtained with 1 x 10⁵ protoplasts ml⁻¹, 25 μg batch⁻¹ of plasmid vector, and 30% polyethylene glycol 4,000. |
| doi_str_mv | 10.1007/s11240-009-9564-4 |
| format | Article |
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Adventitious shoots were formed from both leaf explants and suspension-cultured cells in a Murashige and Skoog (MS) (Physiol Plant 15:473-497, 1962) basal salts containing 500 mg l⁻¹ casamino acids, and supplemented with 5.4 μM a-naphthaleneacetic acid (NAA) and 4.7 μM kinetin to the culture medium. Hyperhydricity of shoots was avoided by increasing the ventilation of the culture vessel. Root formation from shoots was promoted in the presence of 26.9 μM NAA. A high yield of protoplasts was isolated using 1% cellulase and 0.25% pectinase from both leaf mesophyll and suspension-cultured cells, and these were used for transient expression. The highest level of transient expression of the green fluorescent protein was obtained with 1 x 10⁵ protoplasts ml⁻¹, 25 μg batch⁻¹ of plasmid vector, and 30% polyethylene glycol 4,000.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-009-9564-4</identifier><identifier>CODEN: PTCEDJ</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>adventitious shoots ; amino acids ; Aster tripolium ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Callus ; callus culture ; callus formation ; Cell culture ; cell suspension culture ; Cellulase ; culture media ; Explants ; Fluorescence ; Fundamental and applied biological sciences. Psychology ; gene expression ; Genetic resources ; Green fluorescent protein ; Halophytes ; hyperhydricity ; in vitro regeneration ; Kinetin ; Leaves ; Life Sciences ; Mesophyll ; Naphthaleneacetic acid ; new methods ; Original Paper ; Pectinase ; plant genetic resources ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; plasmid vectors ; Polyethylene glycol ; Protoplasts ; Regeneration ; rooting ; Salt tolerance ; Salts ; Shoots ; Tripolium ; Ventilation</subject><ispartof>Plant cell, tissue and organ culture, 2009-09, Vol.98 (3), p.303-309</ispartof><rights>Springer Science+Business Media B.V. 2009</rights><rights>2009 INIST-CNRS</rights><rights>Plant Cell, Tissue and Organ Culture (PCTOC) is a copyright of Springer, (2009). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-fa2413ea06072d65b09afd0daaa1c085c43ae6c89cf2fa872f2b719e8e1337683</citedby><cites>FETCH-LOGICAL-c402t-fa2413ea06072d65b09afd0daaa1c085c43ae6c89cf2fa872f2b719e8e1337683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-009-9564-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-009-9564-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21846415$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Uno, Yuichi</creatorcontrib><creatorcontrib>Nakao, Shigeyuki</creatorcontrib><creatorcontrib>Yamai, Yumiko</creatorcontrib><creatorcontrib>Koyama, Ryohei</creatorcontrib><creatorcontrib>Kanechi, Michio</creatorcontrib><creatorcontrib>Inagaki, Noboru</creatorcontrib><title>Callus formation, plant regeneration, and transient expression in the halophyte sea aster (Aster tripolium L.)</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>An in vitro regeneration and transient expression systems were developed for the halophyte sea aster (Aster tripolium L.), an important genetic resource for salt tolerance. Adventitious shoots were formed from both leaf explants and suspension-cultured cells in a Murashige and Skoog (MS) (Physiol Plant 15:473-497, 1962) basal salts containing 500 mg l⁻¹ casamino acids, and supplemented with 5.4 μM a-naphthaleneacetic acid (NAA) and 4.7 μM kinetin to the culture medium. Hyperhydricity of shoots was avoided by increasing the ventilation of the culture vessel. Root formation from shoots was promoted in the presence of 26.9 μM NAA. A high yield of protoplasts was isolated using 1% cellulase and 0.25% pectinase from both leaf mesophyll and suspension-cultured cells, and these were used for transient expression. The highest level of transient expression of the green fluorescent protein was obtained with 1 x 10⁵ protoplasts ml⁻¹, 25 μg batch⁻¹ of plasmid vector, and 30% polyethylene glycol 4,000.</description><subject>adventitious shoots</subject><subject>amino acids</subject><subject>Aster tripolium</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Callus</subject><subject>callus culture</subject><subject>callus formation</subject><subject>Cell culture</subject><subject>cell suspension culture</subject><subject>Cellulase</subject><subject>culture media</subject><subject>Explants</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Genetic resources</subject><subject>Green fluorescent protein</subject><subject>Halophytes</subject><subject>hyperhydricity</subject><subject>in vitro regeneration</subject><subject>Kinetin</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Mesophyll</subject><subject>Naphthaleneacetic acid</subject><subject>new methods</subject><subject>Original Paper</subject><subject>Pectinase</subject><subject>plant genetic resources</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>plasmid vectors</subject><subject>Polyethylene glycol</subject><subject>Protoplasts</subject><subject>Regeneration</subject><subject>rooting</subject><subject>Salt tolerance</subject><subject>Salts</subject><subject>Shoots</subject><subject>Tripolium</subject><subject>Ventilation</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUuLFDEQx4O44DjrB_BkQEQFe628u4_L4AsGPOieQ22mMtNLT3ebdIP77TezPSh48FSQ_4NK_Rh7KeBKALiPWQipoQJoqsZYXeknbCWMU5UBrZ-yFQjrKlsb94w9z_kOAKzSYsX6DXbdnHkc0hGndug_8LHDfuKJ9tRTOr9hv-NTwj63VDT6PSbKuSi87fl0IH7AbhgP9xPxTMgxT5T4u-vHMaV2HLp2PvLt1ftLdhGxy_TiPNfs5vOnn5uv1fb7l2-b620VNMipiii1UIRgwcmdNbfQYNzBDhFFgNoErZBsqJsQZcTayShvnWioJqGUs7Vas7dL75iGXzPlyR_bHKgrX6Nhzr4Bqay05Qhr9vof590wp74s56U0japlo099YnGFNOScKPoxtUdM916APwHwCwBfAPgTAK9L5s25GXPALpbzhTb_CUpRa6uFKT65-HKR-j2lvxv8r_zVEoo4eNynUnzzQ4JQBbR1YKx6AGafnso</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Uno, Yuichi</creator><creator>Nakao, Shigeyuki</creator><creator>Yamai, Yumiko</creator><creator>Koyama, Ryohei</creator><creator>Kanechi, Michio</creator><creator>Inagaki, Noboru</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20090901</creationdate><title>Callus formation, plant regeneration, and transient expression in the halophyte sea aster (Aster tripolium L.)</title><author>Uno, Yuichi ; Nakao, Shigeyuki ; Yamai, Yumiko ; Koyama, Ryohei ; Kanechi, Michio ; Inagaki, Noboru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-fa2413ea06072d65b09afd0daaa1c085c43ae6c89cf2fa872f2b719e8e1337683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>adventitious shoots</topic><topic>amino acids</topic><topic>Aster tripolium</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Callus</topic><topic>callus culture</topic><topic>callus formation</topic><topic>Cell culture</topic><topic>cell suspension culture</topic><topic>Cellulase</topic><topic>culture media</topic><topic>Explants</topic><topic>Fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Genetic resources</topic><topic>Green fluorescent protein</topic><topic>Halophytes</topic><topic>hyperhydricity</topic><topic>in vitro regeneration</topic><topic>Kinetin</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Mesophyll</topic><topic>Naphthaleneacetic acid</topic><topic>new methods</topic><topic>Original Paper</topic><topic>Pectinase</topic><topic>plant genetic resources</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>plasmid vectors</topic><topic>Polyethylene glycol</topic><topic>Protoplasts</topic><topic>Regeneration</topic><topic>rooting</topic><topic>Salt tolerance</topic><topic>Salts</topic><topic>Shoots</topic><topic>Tripolium</topic><topic>Ventilation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Uno, Yuichi</creatorcontrib><creatorcontrib>Nakao, Shigeyuki</creatorcontrib><creatorcontrib>Yamai, Yumiko</creatorcontrib><creatorcontrib>Koyama, Ryohei</creatorcontrib><creatorcontrib>Kanechi, Michio</creatorcontrib><creatorcontrib>Inagaki, Noboru</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Uno, Yuichi</au><au>Nakao, Shigeyuki</au><au>Yamai, Yumiko</au><au>Koyama, Ryohei</au><au>Kanechi, Michio</au><au>Inagaki, Noboru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Callus formation, plant regeneration, and transient expression in the halophyte sea aster (Aster tripolium L.)</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2009-09-01</date><risdate>2009</risdate><volume>98</volume><issue>3</issue><spage>303</spage><epage>309</epage><pages>303-309</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><coden>PTCEDJ</coden><abstract>An in vitro regeneration and transient expression systems were developed for the halophyte sea aster (Aster tripolium L.), an important genetic resource for salt tolerance. Adventitious shoots were formed from both leaf explants and suspension-cultured cells in a Murashige and Skoog (MS) (Physiol Plant 15:473-497, 1962) basal salts containing 500 mg l⁻¹ casamino acids, and supplemented with 5.4 μM a-naphthaleneacetic acid (NAA) and 4.7 μM kinetin to the culture medium. Hyperhydricity of shoots was avoided by increasing the ventilation of the culture vessel. Root formation from shoots was promoted in the presence of 26.9 μM NAA. A high yield of protoplasts was isolated using 1% cellulase and 0.25% pectinase from both leaf mesophyll and suspension-cultured cells, and these were used for transient expression. The highest level of transient expression of the green fluorescent protein was obtained with 1 x 10⁵ protoplasts ml⁻¹, 25 μg batch⁻¹ of plasmid vector, and 30% polyethylene glycol 4,000.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><doi>10.1007/s11240-009-9564-4</doi><tpages>7</tpages></addata></record> |
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| ispartof | Plant cell, tissue and organ culture, 2009-09, Vol.98 (3), p.303-309 |
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| subjects | adventitious shoots amino acids Aster tripolium Biological and medical sciences Biomedical and Life Sciences Biotechnology Callus callus culture callus formation Cell culture cell suspension culture Cellulase culture media Explants Fluorescence Fundamental and applied biological sciences. Psychology gene expression Genetic resources Green fluorescent protein Halophytes hyperhydricity in vitro regeneration Kinetin Leaves Life Sciences Mesophyll Naphthaleneacetic acid new methods Original Paper Pectinase plant genetic resources Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences plasmid vectors Polyethylene glycol Protoplasts Regeneration rooting Salt tolerance Salts Shoots Tripolium Ventilation |
| title | Callus formation, plant regeneration, and transient expression in the halophyte sea aster (Aster tripolium L.) |
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