In vitro culture of precision-cut testicular tissue as a novel tool for the study of responses to LH

In vitro culture systems are valuable tools for investigating reproductive mechanisms in the testis. Here, we report the use of the precision-cut in vitro system using equine testicular slices. Testes were collected from immature light breed stallions (n = 3) and cut into slices (mean slice weight =...

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Veröffentlicht in:	In vitro cellular & developmental biology. Animal 2010-01, Vol.46 (1), p.45-53
Hauptverfasser:	Laughlin, Andy Michael, Welsh, Thomas H. Jr, Love, Charles C, Varner, Dickson D, Parrish, Alan R, Forrest, David W, Ing, Nancy H
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Genetics and Genomics Animals Biomedical and Life Sciences CELL AND TISSUE MODELS Cell Biology Cell Culture Cell Shape - drug effects Cultured cells Cyclic Nucleotide Phosphodiesterases, Type 3 - genetics Cyclic Nucleotide Phosphodiesterases, Type 3 - metabolism Developmental Biology Estradiol - biosynthesis Fibers Gene expression Gene Expression Regulation - drug effects Germ cells Heat-Shock Proteins - genetics Heat-Shock Proteins - metabolism Hormones Horses In Situ Hybridization L-Lactate dehydrogenase L-Lactate Dehydrogenase - secretion Leydig cells Life Sciences Light effects Luteinizing hormone Luteinizing Hormone - pharmacology Male Messenger RNA phosphodiesterase Phosphoproteins - genetics Phosphoproteins - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Seminiferous tubule Sheep Sperm Stem Cells Steroidogenesis Steroidogenic acute regulatory protein Testes Testis - cytology Testis - drug effects Testis - enzymology Testosterone Testosterone - biosynthesis Tissue culture techniques Tissue Culture Techniques - methods
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