Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus)
A new marine fish cell line, TK, derived from turbot (Scophthalmus maximus) kidney, was established by the method of trypsin digestion and subcultured for more than 50 passages over a period of 300 days. The TK cells were maintained in Minimum Essential Medium Eagle (MEM) supplemented with HEPES, an...
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| Veröffentlicht in: | Fish physiology and biochemistry 2010-12, Vol.36 (4), p.1227-1234 |
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| creator | Wang, N Wang, X. L Sha, Z. X Tian, Y. S Chen, S. L |
| description | A new marine fish cell line, TK, derived from turbot (Scophthalmus maximus) kidney, was established by the method of trypsin digestion and subcultured for more than 50 passages over a period of 300 days. The TK cells were maintained in Minimum Essential Medium Eagle (MEM) supplemented with HEPES, antibiotics, fetal bovine serum (FBS), 2-Mercaptoethanol (2-Me), and basic fibroblast growth factor (bFGF). The suitable growth temperature for TK cells was 24°C, and microscopically, TK cells were composed of fibroblast-like cells. Chromosome analysis revealed that the TK cell line has a normal diploid karyotype with 2n = 44. Two fish viruses LCDV-C (lymphocystis disease virus from China) and TRBIV (turbot reddish body iridovirus) were used to determine the virus susceptibility of TK cell line. The TK cell line was found to be susceptible to TRBIV, and the infection was confirmed by cytopathic effect (CPE) and transmission electron microscopy, which detected the viral particles in the cytoplasm of virus-infected cells. Finally, significant green fluorescent signals were observed when the TK cells were transfected with pEGFP-N3 vector, indicating its potential utility for fish virus study and genetic manipulation. |
| doi_str_mv | 10.1007/s10695-010-9402-y |
| format | Article |
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L ; Sha, Z. X ; Tian, Y. S ; Chen, S. L</creator><creatorcontrib>Wang, N ; Wang, X. L ; Sha, Z. X ; Tian, Y. S ; Chen, S. L</creatorcontrib><description>A new marine fish cell line, TK, derived from turbot (Scophthalmus maximus) kidney, was established by the method of trypsin digestion and subcultured for more than 50 passages over a period of 300 days. The TK cells were maintained in Minimum Essential Medium Eagle (MEM) supplemented with HEPES, antibiotics, fetal bovine serum (FBS), 2-Mercaptoethanol (2-Me), and basic fibroblast growth factor (bFGF). The suitable growth temperature for TK cells was 24°C, and microscopically, TK cells were composed of fibroblast-like cells. Chromosome analysis revealed that the TK cell line has a normal diploid karyotype with 2n = 44. Two fish viruses LCDV-C (lymphocystis disease virus from China) and TRBIV (turbot reddish body iridovirus) were used to determine the virus susceptibility of TK cell line. The TK cell line was found to be susceptible to TRBIV, and the infection was confirmed by cytopathic effect (CPE) and transmission electron microscopy, which detected the viral particles in the cytoplasm of virus-infected cells. Finally, significant green fluorescent signals were observed when the TK cells were transfected with pEGFP-N3 vector, indicating its potential utility for fish virus study and genetic manipulation.</description><identifier>ISSN: 0920-1742</identifier><identifier>EISSN: 1573-5168</identifier><identifier>DOI: 10.1007/s10695-010-9402-y</identifier><identifier>PMID: 20496112</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Animal Physiology ; Animals ; Antibiotics ; Biomedical and Life Sciences ; Cell culture ; Cell Culture Techniques - methods ; Cell Line ; Chromosomes ; Disease ; Disease Susceptibility - virology ; Fish ; Fish diseases ; Fisheries ; Flatfishes ; Freshwater & Marine Ecology ; Genes ; Genetic engineering ; Green Fluorescent Proteins - metabolism ; Growth factors ; Histology ; Immunology ; Iridovirus ; Iridovirus - ultrastructure ; Karyotype ; Karyotypes ; Karyotyping ; Kidney - cytology ; Kidney cell line ; Life Sciences ; Lymphocystis disease virus ; Marine ; Marine fish ; Microscopy, Electron, Transmission ; Morphology ; Original Paper ; Psetta maxima ; Scophthalmus maximus ; Temperature ; Transfection ; Trypsin ; turbot ; Virology ; Virus susceptibility ; Viruses ; Zoology</subject><ispartof>Fish physiology and biochemistry, 2010-12, Vol.36 (4), p.1227-1234</ispartof><rights>Springer Science+Business Media B.V. 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-8381efac4b0abd2414cd572b2c9bbbcb118a651369ed9fd27100253e2f9da7bc3</citedby><cites>FETCH-LOGICAL-c426t-8381efac4b0abd2414cd572b2c9bbbcb118a651369ed9fd27100253e2f9da7bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10695-010-9402-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10695-010-9402-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20496112$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, N</creatorcontrib><creatorcontrib>Wang, X. L</creatorcontrib><creatorcontrib>Sha, Z. X</creatorcontrib><creatorcontrib>Tian, Y. S</creatorcontrib><creatorcontrib>Chen, S. L</creatorcontrib><title>Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus)</title><title>Fish physiology and biochemistry</title><addtitle>Fish Physiol Biochem</addtitle><addtitle>Fish Physiol Biochem</addtitle><description>A new marine fish cell line, TK, derived from turbot (Scophthalmus maximus) kidney, was established by the method of trypsin digestion and subcultured for more than 50 passages over a period of 300 days. The TK cells were maintained in Minimum Essential Medium Eagle (MEM) supplemented with HEPES, antibiotics, fetal bovine serum (FBS), 2-Mercaptoethanol (2-Me), and basic fibroblast growth factor (bFGF). The suitable growth temperature for TK cells was 24°C, and microscopically, TK cells were composed of fibroblast-like cells. Chromosome analysis revealed that the TK cell line has a normal diploid karyotype with 2n = 44. Two fish viruses LCDV-C (lymphocystis disease virus from China) and TRBIV (turbot reddish body iridovirus) were used to determine the virus susceptibility of TK cell line. The TK cell line was found to be susceptible to TRBIV, and the infection was confirmed by cytopathic effect (CPE) and transmission electron microscopy, which detected the viral particles in the cytoplasm of virus-infected cells. Finally, significant green fluorescent signals were observed when the TK cells were transfected with pEGFP-N3 vector, indicating its potential utility for fish virus study and genetic manipulation.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animal Physiology</subject><subject>Animals</subject><subject>Antibiotics</subject><subject>Biomedical and Life Sciences</subject><subject>Cell culture</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Line</subject><subject>Chromosomes</subject><subject>Disease</subject><subject>Disease Susceptibility - virology</subject><subject>Fish</subject><subject>Fish diseases</subject><subject>Fisheries</subject><subject>Flatfishes</subject><subject>Freshwater & Marine Ecology</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Growth factors</subject><subject>Histology</subject><subject>Immunology</subject><subject>Iridovirus</subject><subject>Iridovirus - ultrastructure</subject><subject>Karyotype</subject><subject>Karyotypes</subject><subject>Karyotyping</subject><subject>Kidney - cytology</subject><subject>Kidney cell line</subject><subject>Life Sciences</subject><subject>Lymphocystis disease virus</subject><subject>Marine</subject><subject>Marine fish</subject><subject>Microscopy, Electron, Transmission</subject><subject>Morphology</subject><subject>Original Paper</subject><subject>Psetta maxima</subject><subject>Scophthalmus maximus</subject><subject>Temperature</subject><subject>Transfection</subject><subject>Trypsin</subject><subject>turbot</subject><subject>Virology</subject><subject>Virus susceptibility</subject><subject>Viruses</subject><subject>Zoology</subject><issn>0920-1742</issn><issn>1573-5168</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU9v1DAQxS0EotuFD8AFLC6FQ2DGduz4iMpfqRKH0rNlO043VRIvdgIsnx4vKSBxgNPImt97M55HyCOEFwigXmYEqesKECotgFWHO2SDteJVjbK5SzagGVSoBDshpznfAIBWEu-TEwZCS0S2Ie51-BKGuB_DNFM7tdTvbLJ-Dqn_buc-TjR21NIpfKWjTf0UaNfnHfVhGOjw85niSOcluTjTZ5c-7nfzzg7jkgv_rS_1-QNyr7NDDg9v65ZcvX3z6fx9dfHx3YfzVxeVF0zOVcMbDJ31woF1LRMofFsr5pjXzjnvEBsra-RSh1Z3LVPlBKzmgXW6tcp5viVnq-8-xc9LyLMZ-3xc1E4hLtloYFyi4vq_pJJMNoKLppBP_yJv4pKm8g3T1LIulsV0S3CFfIo5p9CZferLtQ4GwRyDMmtQpgRljkGZQ9E8vjVe3Bja34pfyRSArUAurek6pD-T_-X6ZBV1Nhp7nfpsri4ZIAfUoDRT_AfLkKfR</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>Wang, N</creator><creator>Wang, X. 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L</au><au>Sha, Z. X</au><au>Tian, Y. S</au><au>Chen, S. L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus)</atitle><jtitle>Fish physiology and biochemistry</jtitle><stitle>Fish Physiol Biochem</stitle><addtitle>Fish Physiol Biochem</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>36</volume><issue>4</issue><spage>1227</spage><epage>1234</epage><pages>1227-1234</pages><issn>0920-1742</issn><eissn>1573-5168</eissn><abstract>A new marine fish cell line, TK, derived from turbot (Scophthalmus maximus) kidney, was established by the method of trypsin digestion and subcultured for more than 50 passages over a period of 300 days. The TK cells were maintained in Minimum Essential Medium Eagle (MEM) supplemented with HEPES, antibiotics, fetal bovine serum (FBS), 2-Mercaptoethanol (2-Me), and basic fibroblast growth factor (bFGF). The suitable growth temperature for TK cells was 24°C, and microscopically, TK cells were composed of fibroblast-like cells. Chromosome analysis revealed that the TK cell line has a normal diploid karyotype with 2n = 44. Two fish viruses LCDV-C (lymphocystis disease virus from China) and TRBIV (turbot reddish body iridovirus) were used to determine the virus susceptibility of TK cell line. The TK cell line was found to be susceptible to TRBIV, and the infection was confirmed by cytopathic effect (CPE) and transmission electron microscopy, which detected the viral particles in the cytoplasm of virus-infected cells. Finally, significant green fluorescent signals were observed when the TK cells were transfected with pEGFP-N3 vector, indicating its potential utility for fish virus study and genetic manipulation.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><pmid>20496112</pmid><doi>10.1007/s10695-010-9402-y</doi><tpages>8</tpages></addata></record> |
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| subjects | Animal Anatomy Animal Biochemistry Animal Physiology Animals Antibiotics Biomedical and Life Sciences Cell culture Cell Culture Techniques - methods Cell Line Chromosomes Disease Disease Susceptibility - virology Fish Fish diseases Fisheries Flatfishes Freshwater & Marine Ecology Genes Genetic engineering Green Fluorescent Proteins - metabolism Growth factors Histology Immunology Iridovirus Iridovirus - ultrastructure Karyotype Karyotypes Karyotyping Kidney - cytology Kidney cell line Life Sciences Lymphocystis disease virus Marine Marine fish Microscopy, Electron, Transmission Morphology Original Paper Psetta maxima Scophthalmus maximus Temperature Transfection Trypsin turbot Virology Virus susceptibility Viruses Zoology |
| title | Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus) |
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