Effect of putrescine on the conversion of protocorm-like bodies of Dendrobium officinale to shoots
The addition of 1.5 mM putrescine to the culture medium facilitated the conversion of protocorm-like bodies (PLBs) of Dendrobium officinale to shoots. The administration of 1.0 mM of the polyamine inhibitor, α-DL-difluoromethylarginine, decreased the conversion of PLBs to shoots. Compared to the con...
Gespeichert in:
| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2010-08, Vol.102 (2), p.145-151 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 151 |
|---|---|
| container_issue | 2 |
| container_start_page | 145 |
| container_title | Plant cell, tissue and organ culture |
| container_volume | 102 |
| creator | Wei, Ming Wei, Sheng-Hua Yang, Chao-Ying |
| description | The addition of 1.5 mM putrescine to the culture medium facilitated the conversion of protocorm-like bodies (PLBs) of Dendrobium officinale to shoots. The administration of 1.0 mM of the polyamine inhibitor, α-DL-difluoromethylarginine, decreased the conversion of PLBs to shoots. Compared to the control, the PLBs treated with 1.5 mM putrescine had higher levels of total intracellular free polyamines. The enhanced conversion of PLBs to shoots following the addition of putrescine was accompanied by an increase in the ratio of total cytokinins (CTKs) to indole-3-acetic acid (IAA). Analysis of enzyme activities indicated that the increased endogenous level of total CTKs driven by putrescine was associated with the inhibition of CTK decomposition by CTK oxidase, while the decreased endogenous level of IAA was associated with the promotion of IAA decomposition by IAA oxidase. In addition, putrescine increased the ratio of reduced glutathione to oxidized glutathione in the conversion process of PLBs to shoots. |
| doi_str_mv | 10.1007/s11240-010-9715-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_902359025</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2259382060</sourcerecordid><originalsourceid>FETCH-LOGICAL-c402t-a5a11b5400f1837e4442a733c7723288e8209047cd96a64abe3d901e1b5980a63</originalsourceid><addsrcrecordid>eNp9kEtv1TAQhS0EEpfCD2BFJIRYmY5fcbJEpTykSl20XVuO77hNyY2LJ0Hi3zNXqUBiwcbW6HzneHyEeK3ggwLwp6SUtiBBgey9ctI_ETvlvJEOrH0qdqBaL9vO-efiBdE9ALTGqp0YznPGtDQlNw_rUpHSOGNT5ma5wyaV-SdWGnk86rUsJZV6kNP4HZuh7Eeko_AJ530tw7geeMojJ8QJm6U0dFfKQi_FsxwnwleP94m4-Xx-ffZVXlx--Xb28UImC3qR0UWlBmcBsuqMR2utjt6Y5L02uuuw09CD9Wnft7G1cUCz70Ehe_oOYmtOxPstlxf9sSIt4TBSwmmKM5aVQg_aOD4ck2__Ie_LWnlrClq73vBLLTClNirVQlQxh4c6HmL9FRSEY-lhKz1w6eFYevDsefeYHCnFKdc4p5H-GLXuO_6gYk5vHLE032L9u8H_wt9sphxLiLeVg2-uNKeB6rgD7c1vfUCZRA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2259382060</pqid></control><display><type>article</type><title>Effect of putrescine on the conversion of protocorm-like bodies of Dendrobium officinale to shoots</title><source>Springer Nature - Complete Springer Journals</source><creator>Wei, Ming ; Wei, Sheng-Hua ; Yang, Chao-Ying</creator><creatorcontrib>Wei, Ming ; Wei, Sheng-Hua ; Yang, Chao-Ying</creatorcontrib><description>The addition of 1.5 mM putrescine to the culture medium facilitated the conversion of protocorm-like bodies (PLBs) of Dendrobium officinale to shoots. The administration of 1.0 mM of the polyamine inhibitor, α-DL-difluoromethylarginine, decreased the conversion of PLBs to shoots. Compared to the control, the PLBs treated with 1.5 mM putrescine had higher levels of total intracellular free polyamines. The enhanced conversion of PLBs to shoots following the addition of putrescine was accompanied by an increase in the ratio of total cytokinins (CTKs) to indole-3-acetic acid (IAA). Analysis of enzyme activities indicated that the increased endogenous level of total CTKs driven by putrescine was associated with the inhibition of CTK decomposition by CTK oxidase, while the decreased endogenous level of IAA was associated with the promotion of IAA decomposition by IAA oxidase. In addition, putrescine increased the ratio of reduced glutathione to oxidized glutathione in the conversion process of PLBs to shoots.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-010-9715-7</identifier><identifier>CODEN: PTCEDJ</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Acetic acid ; adventitious shoots ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Conversion ; corms ; culture media ; cytokinin oxidase ; Cytokinins ; Decomposition ; Dendrobium ; Dendrobium officinale ; Enzymatic activity ; enzyme activity ; Fundamental and applied biological sciences. Psychology ; Glutathione ; IAA oxidase ; in vitro regeneration ; indole acetic acid ; Indoleacetic acid ; Life Sciences ; micropropagation ; Original Paper ; Oxidase ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Polyamines ; Protocorm-like bodies ; Putrescine ; Shoots</subject><ispartof>Plant cell, tissue and organ culture, 2010-08, Vol.102 (2), p.145-151</ispartof><rights>Springer Science+Business Media B.V. 2010</rights><rights>2015 INIST-CNRS</rights><rights>Plant Cell, Tissue and Organ Culture (PCTOC) is a copyright of Springer, (2010). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-a5a11b5400f1837e4442a733c7723288e8209047cd96a64abe3d901e1b5980a63</citedby><cites>FETCH-LOGICAL-c402t-a5a11b5400f1837e4442a733c7723288e8209047cd96a64abe3d901e1b5980a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-010-9715-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-010-9715-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22984001$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Wei, Ming</creatorcontrib><creatorcontrib>Wei, Sheng-Hua</creatorcontrib><creatorcontrib>Yang, Chao-Ying</creatorcontrib><title>Effect of putrescine on the conversion of protocorm-like bodies of Dendrobium officinale to shoots</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>The addition of 1.5 mM putrescine to the culture medium facilitated the conversion of protocorm-like bodies (PLBs) of Dendrobium officinale to shoots. The administration of 1.0 mM of the polyamine inhibitor, α-DL-difluoromethylarginine, decreased the conversion of PLBs to shoots. Compared to the control, the PLBs treated with 1.5 mM putrescine had higher levels of total intracellular free polyamines. The enhanced conversion of PLBs to shoots following the addition of putrescine was accompanied by an increase in the ratio of total cytokinins (CTKs) to indole-3-acetic acid (IAA). Analysis of enzyme activities indicated that the increased endogenous level of total CTKs driven by putrescine was associated with the inhibition of CTK decomposition by CTK oxidase, while the decreased endogenous level of IAA was associated with the promotion of IAA decomposition by IAA oxidase. In addition, putrescine increased the ratio of reduced glutathione to oxidized glutathione in the conversion process of PLBs to shoots.</description><subject>Acetic acid</subject><subject>adventitious shoots</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Conversion</subject><subject>corms</subject><subject>culture media</subject><subject>cytokinin oxidase</subject><subject>Cytokinins</subject><subject>Decomposition</subject><subject>Dendrobium</subject><subject>Dendrobium officinale</subject><subject>Enzymatic activity</subject><subject>enzyme activity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutathione</subject><subject>IAA oxidase</subject><subject>in vitro regeneration</subject><subject>indole acetic acid</subject><subject>Indoleacetic acid</subject><subject>Life Sciences</subject><subject>micropropagation</subject><subject>Original Paper</subject><subject>Oxidase</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Polyamines</subject><subject>Protocorm-like bodies</subject><subject>Putrescine</subject><subject>Shoots</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kEtv1TAQhS0EEpfCD2BFJIRYmY5fcbJEpTykSl20XVuO77hNyY2LJ0Hi3zNXqUBiwcbW6HzneHyEeK3ggwLwp6SUtiBBgey9ctI_ETvlvJEOrH0qdqBaL9vO-efiBdE9ALTGqp0YznPGtDQlNw_rUpHSOGNT5ma5wyaV-SdWGnk86rUsJZV6kNP4HZuh7Eeko_AJ530tw7geeMojJ8QJm6U0dFfKQi_FsxwnwleP94m4-Xx-ffZVXlx--Xb28UImC3qR0UWlBmcBsuqMR2utjt6Y5L02uuuw09CD9Wnft7G1cUCz70Ehe_oOYmtOxPstlxf9sSIt4TBSwmmKM5aVQg_aOD4ck2__Ie_LWnlrClq73vBLLTClNirVQlQxh4c6HmL9FRSEY-lhKz1w6eFYevDsefeYHCnFKdc4p5H-GLXuO_6gYk5vHLE032L9u8H_wt9sphxLiLeVg2-uNKeB6rgD7c1vfUCZRA</recordid><startdate>20100801</startdate><enddate>20100801</enddate><creator>Wei, Ming</creator><creator>Wei, Sheng-Hua</creator><creator>Yang, Chao-Ying</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100801</creationdate><title>Effect of putrescine on the conversion of protocorm-like bodies of Dendrobium officinale to shoots</title><author>Wei, Ming ; Wei, Sheng-Hua ; Yang, Chao-Ying</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-a5a11b5400f1837e4442a733c7723288e8209047cd96a64abe3d901e1b5980a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acetic acid</topic><topic>adventitious shoots</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Conversion</topic><topic>corms</topic><topic>culture media</topic><topic>cytokinin oxidase</topic><topic>Cytokinins</topic><topic>Decomposition</topic><topic>Dendrobium</topic><topic>Dendrobium officinale</topic><topic>Enzymatic activity</topic><topic>enzyme activity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutathione</topic><topic>IAA oxidase</topic><topic>in vitro regeneration</topic><topic>indole acetic acid</topic><topic>Indoleacetic acid</topic><topic>Life Sciences</topic><topic>micropropagation</topic><topic>Original Paper</topic><topic>Oxidase</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Polyamines</topic><topic>Protocorm-like bodies</topic><topic>Putrescine</topic><topic>Shoots</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Ming</creatorcontrib><creatorcontrib>Wei, Sheng-Hua</creatorcontrib><creatorcontrib>Yang, Chao-Ying</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Ming</au><au>Wei, Sheng-Hua</au><au>Yang, Chao-Ying</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of putrescine on the conversion of protocorm-like bodies of Dendrobium officinale to shoots</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2010-08-01</date><risdate>2010</risdate><volume>102</volume><issue>2</issue><spage>145</spage><epage>151</epage><pages>145-151</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><coden>PTCEDJ</coden><abstract>The addition of 1.5 mM putrescine to the culture medium facilitated the conversion of protocorm-like bodies (PLBs) of Dendrobium officinale to shoots. The administration of 1.0 mM of the polyamine inhibitor, α-DL-difluoromethylarginine, decreased the conversion of PLBs to shoots. Compared to the control, the PLBs treated with 1.5 mM putrescine had higher levels of total intracellular free polyamines. The enhanced conversion of PLBs to shoots following the addition of putrescine was accompanied by an increase in the ratio of total cytokinins (CTKs) to indole-3-acetic acid (IAA). Analysis of enzyme activities indicated that the increased endogenous level of total CTKs driven by putrescine was associated with the inhibition of CTK decomposition by CTK oxidase, while the decreased endogenous level of IAA was associated with the promotion of IAA decomposition by IAA oxidase. In addition, putrescine increased the ratio of reduced glutathione to oxidized glutathione in the conversion process of PLBs to shoots.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><doi>10.1007/s11240-010-9715-7</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-6857 |
| ispartof | Plant cell, tissue and organ culture, 2010-08, Vol.102 (2), p.145-151 |
| issn | 0167-6857 1573-5044 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_902359025 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Acetic acid adventitious shoots Biological and medical sciences Biomedical and Life Sciences Biotechnology Conversion corms culture media cytokinin oxidase Cytokinins Decomposition Dendrobium Dendrobium officinale Enzymatic activity enzyme activity Fundamental and applied biological sciences. Psychology Glutathione IAA oxidase in vitro regeneration indole acetic acid Indoleacetic acid Life Sciences micropropagation Original Paper Oxidase Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Polyamines Protocorm-like bodies Putrescine Shoots |
| title | Effect of putrescine on the conversion of protocorm-like bodies of Dendrobium officinale to shoots |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T00%3A09%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20putrescine%20on%20the%20conversion%20of%20protocorm-like%20bodies%20of%20Dendrobium%20officinale%20to%20shoots&rft.jtitle=Plant%20cell,%20tissue%20and%20organ%20culture&rft.au=Wei,%20Ming&rft.date=2010-08-01&rft.volume=102&rft.issue=2&rft.spage=145&rft.epage=151&rft.pages=145-151&rft.issn=0167-6857&rft.eissn=1573-5044&rft.coden=PTCEDJ&rft_id=info:doi/10.1007/s11240-010-9715-7&rft_dat=%3Cproquest_cross%3E2259382060%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2259382060&rft_id=info:pmid/&rfr_iscdi=true |