Secretory expression of all 16 subtypes of the hemagglutinin 1 protein of influenza A virus in insect cells

► Expression of all 16 subtypes of HA1 of influenza A viruses. ► Development of an efficient and simple purification process. ► Potential for large scale production. ► Potential application in immunoassays. Influenza viruses are known for their ability to change their antigenic structure and create...

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Veröffentlicht in:	Journal of virological methods 2011-11, Vol.177 (2), p.160-167
Hauptverfasser:	Cui, Shujuan, Wu, Chao, Zhou, Hongli, Zhao, Rongmao, Guo, Li, Wang, Jianwei, Hung, Tao
Format:	Artikel
Sprache:	eng
Schlagworte:	affinity chromatography Amino Acid Sequence Animals antibodies Antibodies, Viral - immunology Antibodies, Viral - metabolism antigens Autographa californica Autographa californica multiple nucleopolyhedrovirus Baculoviridae - genetics Baculoviridae - metabolism Baculovirus Baculovirus vector Base Sequence Biological and medical sciences Cross Reactions disease outbreaks Female Fundamental and applied biological sciences. Psychology Genetic Vectors HEK293 Cells Hemagglutinin Hemagglutinin Glycoproteins, Influenza Virus - genetics Hemagglutinin Glycoproteins, Influenza Virus - isolation & purification Hemagglutinin Glycoproteins, Influenza Virus - metabolism hemagglutinins Humans Influenza A virus Influenza A virus - genetics Influenza A virus - immunology Influenza A virus - metabolism insect viruses Insecta - cytology insects membrane glycoproteins Mice Mice, Inbred BALB C Microbiology Molecular Sequence Data Nuclear polyhedrosis virus Orthomyxoviridae pathogenesis protein synthesis Purification recombinant proteins Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - secretion Secretory expression signal peptide Techniques used in virology Virology Western blotting
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