Identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal RNA gene sequence analysis

DNA sequence‐based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16S and internal transcribed spacer (ITS) ribosomal RNA gene sequence analysis. PCR was positive for 24 of 99 samples tested. Both culture and 16S rDNA seque...

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Veröffentlicht in:Clinical microbiology and infection 2011-05, Vol.17 (5), p.776-779
Hauptverfasser: Daroy, M.L.G., Lopez, J.S., Torres, B.C.L., Loy, M.J., Tuaño, P.M.C., Matias, R.R.
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container_title Clinical microbiology and infection
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Lopez, J.S.
Torres, B.C.L.
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Tuaño, P.M.C.
Matias, R.R.
description DNA sequence‐based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16S and internal transcribed spacer (ITS) ribosomal RNA gene sequence analysis. PCR was positive for 24 of 99 samples tested. Both culture and 16S rDNA sequence analysis identified Pseudomonas aeruginosa, streptococci and Enterobacteriaceae. Isolates misidentified as Burkholderia cepacia by biochemical tests were identified as Ralstonia mannitolilytica by 16S rDNA sequence analysis. Sequence analysis identified the following microorganisms from 19 culture‐negative samples: Haemophilus influenzae, Sphingomonas sp., Klebsiella pneumoniae, Staphylococcus haemolyticus, Morganella morganii, Mycobacterium sp., Chryseobacterium sp., Pseudomonas saccharophila (Xanthomonas) and the fungus, Phaeoacremonium inflatipes.
doi_str_mv 10.1111/j.1469-0691.2010.03369.x
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subjects 16S rDNA
Biological and medical sciences
Burkholderia cepacia
Chryseobacterium
DNA sequence analysis
DNA, Ribosomal Spacer - genetics
Enterobacteriaceae
Eye
eye infections
Eye Infections, Bacterial - diagnosis
Eye Infections, Fungal - diagnosis
Genes
Haemophilus influenzae
Humans
In Vitro Techniques
Infection
Infectious diseases
internal transcribed spacer rDNA
Klebsiella pneumoniae
Male
Medical research
Medical sciences
Microorganisms
Middle Aged
Morganella morganii
Mycobacterium
Nucleotide sequence
ocular pathogens
Pathogens
Polymerase Chain Reaction
Pseudomonas aeruginosa
Ralstonia mannitolilytica
RNA, Ribosomal, 16S - genetics
rRNA
rRNA 16S
Sequence Analysis, DNA
Spacer
Sphingomonas
Staphylococcus haemolyticus
Xanthomonas
title Identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal RNA gene sequence analysis
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