Identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal RNA gene sequence analysis
DNA sequence‐based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16S and internal transcribed spacer (ITS) ribosomal RNA gene sequence analysis. PCR was positive for 24 of 99 samples tested. Both culture and 16S rDNA seque...
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description | DNA sequence‐based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16S and internal transcribed spacer (ITS) ribosomal RNA gene sequence analysis. PCR was positive for 24 of 99 samples tested. Both culture and 16S rDNA sequence analysis identified Pseudomonas aeruginosa, streptococci and Enterobacteriaceae. Isolates misidentified as Burkholderia cepacia by biochemical tests were identified as Ralstonia mannitolilytica by 16S rDNA sequence analysis. Sequence analysis identified the following microorganisms from 19 culture‐negative samples: Haemophilus influenzae, Sphingomonas sp., Klebsiella pneumoniae, Staphylococcus haemolyticus, Morganella morganii, Mycobacterium sp., Chryseobacterium sp., Pseudomonas saccharophila (Xanthomonas) and the fungus, Phaeoacremonium inflatipes. |
doi_str_mv | 10.1111/j.1469-0691.2010.03369.x |
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PCR was positive for 24 of 99 samples tested. Both culture and 16S rDNA sequence analysis identified Pseudomonas aeruginosa, streptococci and Enterobacteriaceae. Isolates misidentified as Burkholderia cepacia by biochemical tests were identified as Ralstonia mannitolilytica by 16S rDNA sequence analysis. Sequence analysis identified the following microorganisms from 19 culture‐negative samples: Haemophilus influenzae, Sphingomonas sp., Klebsiella pneumoniae, Staphylococcus haemolyticus, Morganella morganii, Mycobacterium sp., Chryseobacterium sp., Pseudomonas saccharophila (Xanthomonas) and the fungus, Phaeoacremonium inflatipes.</description><identifier>ISSN: 1198-743X</identifier><identifier>EISSN: 1469-0691</identifier><identifier>DOI: 10.1111/j.1469-0691.2010.03369.x</identifier><identifier>PMID: 21521414</identifier><language>eng</language><publisher>Oxford, UK: Elsevier Ltd</publisher><subject>16S rDNA ; Biological and medical sciences ; Burkholderia cepacia ; Chryseobacterium ; DNA sequence analysis ; DNA, Ribosomal Spacer - genetics ; Enterobacteriaceae ; Eye ; eye infections ; Eye Infections, Bacterial - diagnosis ; Eye Infections, Fungal - diagnosis ; Genes ; Haemophilus influenzae ; Humans ; In Vitro Techniques ; Infection ; Infectious diseases ; internal transcribed spacer rDNA ; Klebsiella pneumoniae ; Male ; Medical research ; Medical sciences ; Microorganisms ; Middle Aged ; Morganella morganii ; Mycobacterium ; Nucleotide sequence ; ocular pathogens ; Pathogens ; Polymerase Chain Reaction ; Pseudomonas aeruginosa ; Ralstonia mannitolilytica ; RNA, Ribosomal, 16S - genetics ; rRNA ; rRNA 16S ; Sequence Analysis, DNA ; Spacer ; Sphingomonas ; Staphylococcus haemolyticus ; Xanthomonas</subject><ispartof>Clinical microbiology and infection, 2011-05, Vol.17 (5), p.776-779</ispartof><rights>2011 European Society of Clinical Infectious Diseases</rights><rights>2010 The Authors. Clinical Microbiology and Infection © 2010 European Society of Clinical Microbiology and Infectious Diseases</rights><rights>2015 INIST-CNRS</rights><rights>2010 The Authors. 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PCR was positive for 24 of 99 samples tested. Both culture and 16S rDNA sequence analysis identified Pseudomonas aeruginosa, streptococci and Enterobacteriaceae. Isolates misidentified as Burkholderia cepacia by biochemical tests were identified as Ralstonia mannitolilytica by 16S rDNA sequence analysis. Sequence analysis identified the following microorganisms from 19 culture‐negative samples: Haemophilus influenzae, Sphingomonas sp., Klebsiella pneumoniae, Staphylococcus haemolyticus, Morganella morganii, Mycobacterium sp., Chryseobacterium sp., Pseudomonas saccharophila (Xanthomonas) and the fungus, Phaeoacremonium inflatipes.</description><subject>16S rDNA</subject><subject>Biological and medical sciences</subject><subject>Burkholderia cepacia</subject><subject>Chryseobacterium</subject><subject>DNA sequence analysis</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Enterobacteriaceae</subject><subject>Eye</subject><subject>eye infections</subject><subject>Eye Infections, Bacterial - diagnosis</subject><subject>Eye Infections, Fungal - diagnosis</subject><subject>Genes</subject><subject>Haemophilus influenzae</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Infection</subject><subject>Infectious diseases</subject><subject>internal transcribed spacer rDNA</subject><subject>Klebsiella pneumoniae</subject><subject>Male</subject><subject>Medical research</subject><subject>Medical sciences</subject><subject>Microorganisms</subject><subject>Middle Aged</subject><subject>Morganella morganii</subject><subject>Mycobacterium</subject><subject>Nucleotide sequence</subject><subject>ocular pathogens</subject><subject>Pathogens</subject><subject>Polymerase Chain Reaction</subject><subject>Pseudomonas aeruginosa</subject><subject>Ralstonia mannitolilytica</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA</subject><subject>rRNA 16S</subject><subject>Sequence Analysis, DNA</subject><subject>Spacer</subject><subject>Sphingomonas</subject><subject>Staphylococcus haemolyticus</subject><subject>Xanthomonas</subject><issn>1198-743X</issn><issn>1469-0691</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhiMEoqXwF5AlhDhlsWPHSQ4cyoqPSgtIqAdulmM7xYvXXjwJbSR-PBN2KRKXkkvG8fOOPXmKgjC6Yvi83K6YkF1JZcdWFcWvlHPZrW7uFae3G_exZl1bNoJ_OSkeAWwppRXn4mFxUrG6YoKJ0-LnhXVx9IM3evQpkjSQKX6L6RpLMwWdyV6PX9OVi0B8JCb4iGgIM4EJ9s6MzhI3O9wbcIEdgEzg4xXJvk-QdjqQzx_PCeYdAfd9ctE4oqMOM3h4XDwYdAD35Pg-Ky7fvrlcvy83n95drM83pZGcd6VoOybrxkhj-9ZySntXNVXDhlrjOLTtq6a1rTRC8q4RemCDrK2uat33Q2crfla8OLTd54Q3gFHtPBgXgo4uTaA6_C11w2R7J9niCQizpeezf8htmjLOBYrVou54yxlFqj1QJieA7Aa1z36n86wYVYtJtVWLMLUIU4tJ9dukusHo0-MBU79z9jb4Rx0Cz4-ABjQyZB2Nh7-cYFywqkbu1YG79sHN_30Btd58WCrMvz7kHRr64V1WYPyi0fqMypVN_u5pfgGcu89l</recordid><startdate>201105</startdate><enddate>201105</enddate><creator>Daroy, M.L.G.</creator><creator>Lopez, J.S.</creator><creator>Torres, B.C.L.</creator><creator>Loy, M.J.</creator><creator>Tuaño, P.M.C.</creator><creator>Matias, R.R.</creator><general>Elsevier Ltd</general><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201105</creationdate><title>Identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal RNA gene sequence analysis</title><author>Daroy, M.L.G. ; Lopez, J.S. ; Torres, B.C.L. ; Loy, M.J. ; Tuaño, P.M.C. ; Matias, R.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6339-4891657c6cdb8d300be27271f5a33408b278d86c463974af1f65da25abbf9d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>16S rDNA</topic><topic>Biological and medical sciences</topic><topic>Burkholderia cepacia</topic><topic>Chryseobacterium</topic><topic>DNA sequence analysis</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Enterobacteriaceae</topic><topic>Eye</topic><topic>eye infections</topic><topic>Eye Infections, Bacterial - diagnosis</topic><topic>Eye Infections, Fungal - diagnosis</topic><topic>Genes</topic><topic>Haemophilus influenzae</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Infection</topic><topic>Infectious diseases</topic><topic>internal transcribed spacer rDNA</topic><topic>Klebsiella pneumoniae</topic><topic>Male</topic><topic>Medical research</topic><topic>Medical sciences</topic><topic>Microorganisms</topic><topic>Middle Aged</topic><topic>Morganella morganii</topic><topic>Mycobacterium</topic><topic>Nucleotide sequence</topic><topic>ocular pathogens</topic><topic>Pathogens</topic><topic>Polymerase Chain Reaction</topic><topic>Pseudomonas aeruginosa</topic><topic>Ralstonia mannitolilytica</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA</topic><topic>rRNA 16S</topic><topic>Sequence Analysis, DNA</topic><topic>Spacer</topic><topic>Sphingomonas</topic><topic>Staphylococcus haemolyticus</topic><topic>Xanthomonas</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Daroy, M.L.G.</creatorcontrib><creatorcontrib>Lopez, J.S.</creatorcontrib><creatorcontrib>Torres, B.C.L.</creatorcontrib><creatorcontrib>Loy, M.J.</creatorcontrib><creatorcontrib>Tuaño, P.M.C.</creatorcontrib><creatorcontrib>Matias, R.R.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Clinical microbiology and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Daroy, M.L.G.</au><au>Lopez, J.S.</au><au>Torres, B.C.L.</au><au>Loy, M.J.</au><au>Tuaño, P.M.C.</au><au>Matias, R.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal RNA gene sequence analysis</atitle><jtitle>Clinical microbiology and infection</jtitle><addtitle>Clin Microbiol Infect</addtitle><date>2011-05</date><risdate>2011</risdate><volume>17</volume><issue>5</issue><spage>776</spage><epage>779</epage><pages>776-779</pages><issn>1198-743X</issn><eissn>1469-0691</eissn><abstract>DNA sequence‐based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16S and internal transcribed spacer (ITS) ribosomal RNA gene sequence analysis. PCR was positive for 24 of 99 samples tested. Both culture and 16S rDNA sequence analysis identified Pseudomonas aeruginosa, streptococci and Enterobacteriaceae. Isolates misidentified as Burkholderia cepacia by biochemical tests were identified as Ralstonia mannitolilytica by 16S rDNA sequence analysis. Sequence analysis identified the following microorganisms from 19 culture‐negative samples: Haemophilus influenzae, Sphingomonas sp., Klebsiella pneumoniae, Staphylococcus haemolyticus, Morganella morganii, Mycobacterium sp., Chryseobacterium sp., Pseudomonas saccharophila (Xanthomonas) and the fungus, Phaeoacremonium inflatipes.</abstract><cop>Oxford, UK</cop><pub>Elsevier Ltd</pub><pmid>21521414</pmid><doi>10.1111/j.1469-0691.2010.03369.x</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 16S rDNA Biological and medical sciences Burkholderia cepacia Chryseobacterium DNA sequence analysis DNA, Ribosomal Spacer - genetics Enterobacteriaceae Eye eye infections Eye Infections, Bacterial - diagnosis Eye Infections, Fungal - diagnosis Genes Haemophilus influenzae Humans In Vitro Techniques Infection Infectious diseases internal transcribed spacer rDNA Klebsiella pneumoniae Male Medical research Medical sciences Microorganisms Middle Aged Morganella morganii Mycobacterium Nucleotide sequence ocular pathogens Pathogens Polymerase Chain Reaction Pseudomonas aeruginosa Ralstonia mannitolilytica RNA, Ribosomal, 16S - genetics rRNA rRNA 16S Sequence Analysis, DNA Spacer Sphingomonas Staphylococcus haemolyticus Xanthomonas |
title | Identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal RNA gene sequence analysis |
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