Identification of QTL for resistance to plum pox virus strains M and D in Lito and Harcot apricot cultivars
Sharka is a severe apricot viral disease caused by the plum pox virus (PPV) and is responsible for large crop losses in many countries. Among the known PPV strains, both PPV-D (Dideron) and PPV-M (Marcus) are virulent in apricot, the latter being the most threatening. An F1 apricot progeny derived f...
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| Veröffentlicht in: | Molecular breeding 2011-03, Vol.27 (3), p.289-299 |
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| creator | Dondini, Luca Lain, Orietta Vendramin, Vera Rizzo, Marisa Vivoli, Davide Adami, Marco Guidarelli, Michela Gaiotti, Federica Palmisano, Francesco Bazzoni, Alessandra Boscia, Donato Geuna, Filippo Tartarini, Stefano Negri, Paola Castellano, Mariantonietta Savino, Vito Bassi, Daniele Testolin, Raffaele |
| description | Sharka is a severe apricot viral disease caused by the plum pox virus (PPV) and is responsible for large crop losses in many countries. Among the known PPV strains, both PPV-D (Dideron) and PPV-M (Marcus) are virulent in apricot, the latter being the most threatening. An F1 apricot progeny derived from Lito, described in the literature as resistant, crossed to the susceptible selection BO81604311 (San Castrese × Reale di Imola) was used to study the genetic control of resistance to PPV. A population of 118 individuals was phenotyped by inoculating both PPV-D and PPV-M strains in replicated seedlings and scored for 3 years. An additional set of 231 seedlings from the same cross was also phenotyped for 2 years. SSR-based linkage maps were used for quantitative trait locus (QTL) analysis. A major QTL of resistance to both PPV-M and PPV-D strains was found in the top half of the Lito linkage group 1, where a QTL was previously described in Stark Earli-Orange, the donor of Lito resistance. The LOD score was considerably enhanced when the recovery of plants from infection was taken into account. The results obtained in Lito were compared with those observed in a second apricot cross progeny (Harcot × Reale di Imola) in which QTL of resistance to sharka were also mapped in the same linkage group 1 for both PPV strains. Several models of resistance to sharka disease are discussed considering the segregation frequencies, the QTL alignment in the two maps and the information gathered from the literature. |
| doi_str_mv | 10.1007/s11032-010-9431-3 |
| format | Article |
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Among the known PPV strains, both PPV-D (Dideron) and PPV-M (Marcus) are virulent in apricot, the latter being the most threatening. An F1 apricot progeny derived from Lito, described in the literature as resistant, crossed to the susceptible selection BO81604311 (San Castrese × Reale di Imola) was used to study the genetic control of resistance to PPV. A population of 118 individuals was phenotyped by inoculating both PPV-D and PPV-M strains in replicated seedlings and scored for 3 years. An additional set of 231 seedlings from the same cross was also phenotyped for 2 years. SSR-based linkage maps were used for quantitative trait locus (QTL) analysis. A major QTL of resistance to both PPV-M and PPV-D strains was found in the top half of the Lito linkage group 1, where a QTL was previously described in Stark Earli-Orange, the donor of Lito resistance. The LOD score was considerably enhanced when the recovery of plants from infection was taken into account. The results obtained in Lito were compared with those observed in a second apricot cross progeny (Harcot × Reale di Imola) in which QTL of resistance to sharka were also mapped in the same linkage group 1 for both PPV strains. Several models of resistance to sharka disease are discussed considering the segregation frequencies, the QTL alignment in the two maps and the information gathered from the literature.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1007/s11032-010-9431-3</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Apricots ; Biomedical and Life Sciences ; Biotechnology ; breeding ; Cultivars ; Disease resistance ; Gene mapping ; Genetic control ; Genetic crosses ; Life Sciences ; Molecular biology ; Plant biology ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Plum pox ; Plum pox virus ; Potyviridae ; Progeny ; Prunus ; Prunus armeniaca L ; QTL analysis ; Quantitative trait loci ; Seedlings ; Sharka ; Strains (organisms) ; Viral diseases ; Viruses</subject><ispartof>Molecular breeding, 2011-03, Vol.27 (3), p.289-299</ispartof><rights>Springer Science+Business Media B.V. 2010</rights><rights>Springer Science+Business Media B.V. 2010.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-5db079229d04d52e755f6192488fc4531cd8af79445bce642fa584c4e05acc23</citedby><cites>FETCH-LOGICAL-c438t-5db079229d04d52e755f6192488fc4531cd8af79445bce642fa584c4e05acc23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11032-010-9431-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11032-010-9431-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Dondini, Luca</creatorcontrib><creatorcontrib>Lain, Orietta</creatorcontrib><creatorcontrib>Vendramin, Vera</creatorcontrib><creatorcontrib>Rizzo, Marisa</creatorcontrib><creatorcontrib>Vivoli, Davide</creatorcontrib><creatorcontrib>Adami, Marco</creatorcontrib><creatorcontrib>Guidarelli, Michela</creatorcontrib><creatorcontrib>Gaiotti, Federica</creatorcontrib><creatorcontrib>Palmisano, Francesco</creatorcontrib><creatorcontrib>Bazzoni, Alessandra</creatorcontrib><creatorcontrib>Boscia, Donato</creatorcontrib><creatorcontrib>Geuna, Filippo</creatorcontrib><creatorcontrib>Tartarini, Stefano</creatorcontrib><creatorcontrib>Negri, Paola</creatorcontrib><creatorcontrib>Castellano, Mariantonietta</creatorcontrib><creatorcontrib>Savino, Vito</creatorcontrib><creatorcontrib>Bassi, Daniele</creatorcontrib><creatorcontrib>Testolin, Raffaele</creatorcontrib><title>Identification of QTL for resistance to plum pox virus strains M and D in Lito and Harcot apricot cultivars</title><title>Molecular breeding</title><addtitle>Mol Breeding</addtitle><description>Sharka is a severe apricot viral disease caused by the plum pox virus (PPV) and is responsible for large crop losses in many countries. Among the known PPV strains, both PPV-D (Dideron) and PPV-M (Marcus) are virulent in apricot, the latter being the most threatening. An F1 apricot progeny derived from Lito, described in the literature as resistant, crossed to the susceptible selection BO81604311 (San Castrese × Reale di Imola) was used to study the genetic control of resistance to PPV. A population of 118 individuals was phenotyped by inoculating both PPV-D and PPV-M strains in replicated seedlings and scored for 3 years. An additional set of 231 seedlings from the same cross was also phenotyped for 2 years. SSR-based linkage maps were used for quantitative trait locus (QTL) analysis. A major QTL of resistance to both PPV-M and PPV-D strains was found in the top half of the Lito linkage group 1, where a QTL was previously described in Stark Earli-Orange, the donor of Lito resistance. The LOD score was considerably enhanced when the recovery of plants from infection was taken into account. The results obtained in Lito were compared with those observed in a second apricot cross progeny (Harcot × Reale di Imola) in which QTL of resistance to sharka were also mapped in the same linkage group 1 for both PPV strains. Several models of resistance to sharka disease are discussed considering the segregation frequencies, the QTL alignment in the two maps and the information gathered from the literature.</description><subject>Apricots</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>breeding</subject><subject>Cultivars</subject><subject>Disease resistance</subject><subject>Gene mapping</subject><subject>Genetic control</subject><subject>Genetic crosses</subject><subject>Life Sciences</subject><subject>Molecular biology</subject><subject>Plant biology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Plum pox</subject><subject>Plum pox virus</subject><subject>Potyviridae</subject><subject>Progeny</subject><subject>Prunus</subject><subject>Prunus armeniaca L</subject><subject>QTL analysis</subject><subject>Quantitative trait loci</subject><subject>Seedlings</subject><subject>Sharka</subject><subject>Strains (organisms)</subject><subject>Viral diseases</subject><subject>Viruses</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kE1r3DAQhk1JoUnaH9BTBT3k5FaftnQM-egGtpSQ7VloZWlR4pU2Gjk0_z4yLgRyyGlm4HmGmbdpvhL8g2Dc_wRCMKMtJrhVnJGWfWiOiehpq3opj2rPJG5Zz9mn5gTgHldHdd1x83AzuFiCD9aUkCJKHt1u1sinjLKDAMVE61BJ6DBOe3RI_9BTyBMgKNmECOg3MnFAlyhEtA4Vm6eVyTYVZA45zNVOYwlPJsPn5qM3I7gv_-tps7m-2lys2vWfXzcX5-vWciZLK4ZtPY5SNWA-COp6IXxHFOVSessFI3aQxveKc7G1ruPUGyG55Q4LYy1lp83ZsvaQ0-PkoOh9AOvG0USXJtAKUyY6JWUlv78h79OUY71NUyoUV7IjM0UWyuYEkJ3X9bG9yc-aYD2Hr5fwdQ1fz-FrVh26OFDZuHP5dfN70rdF8iZps8sB9N87ignDRHGBBWMvz8mPbQ</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>Dondini, Luca</creator><creator>Lain, Orietta</creator><creator>Vendramin, 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B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20110301</creationdate><title>Identification of QTL for resistance to plum pox virus strains M and D in Lito and Harcot apricot cultivars</title><author>Dondini, Luca ; Lain, Orietta ; Vendramin, Vera ; Rizzo, Marisa ; Vivoli, Davide ; Adami, Marco ; Guidarelli, Michela ; Gaiotti, Federica ; Palmisano, Francesco ; Bazzoni, Alessandra ; Boscia, Donato ; Geuna, 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Stefano</creatorcontrib><creatorcontrib>Negri, Paola</creatorcontrib><creatorcontrib>Castellano, Mariantonietta</creatorcontrib><creatorcontrib>Savino, Vito</creatorcontrib><creatorcontrib>Bassi, Daniele</creatorcontrib><creatorcontrib>Testolin, Raffaele</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dondini, Luca</au><au>Lain, Orietta</au><au>Vendramin, Vera</au><au>Rizzo, Marisa</au><au>Vivoli, Davide</au><au>Adami, Marco</au><au>Guidarelli, Michela</au><au>Gaiotti, Federica</au><au>Palmisano, Francesco</au><au>Bazzoni, Alessandra</au><au>Boscia, Donato</au><au>Geuna, Filippo</au><au>Tartarini, Stefano</au><au>Negri, Paola</au><au>Castellano, Mariantonietta</au><au>Savino, Vito</au><au>Bassi, Daniele</au><au>Testolin, Raffaele</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of QTL for resistance to plum pox virus strains M and D in Lito and Harcot apricot cultivars</atitle><jtitle>Molecular breeding</jtitle><stitle>Mol Breeding</stitle><date>2011-03-01</date><risdate>2011</risdate><volume>27</volume><issue>3</issue><spage>289</spage><epage>299</epage><pages>289-299</pages><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>Sharka is a severe apricot viral disease caused by the plum pox virus (PPV) and is responsible for large crop losses in many countries. Among the known PPV strains, both PPV-D (Dideron) and PPV-M (Marcus) are virulent in apricot, the latter being the most threatening. An F1 apricot progeny derived from Lito, described in the literature as resistant, crossed to the susceptible selection BO81604311 (San Castrese × Reale di Imola) was used to study the genetic control of resistance to PPV. A population of 118 individuals was phenotyped by inoculating both PPV-D and PPV-M strains in replicated seedlings and scored for 3 years. An additional set of 231 seedlings from the same cross was also phenotyped for 2 years. SSR-based linkage maps were used for quantitative trait locus (QTL) analysis. A major QTL of resistance to both PPV-M and PPV-D strains was found in the top half of the Lito linkage group 1, where a QTL was previously described in Stark Earli-Orange, the donor of Lito resistance. The LOD score was considerably enhanced when the recovery of plants from infection was taken into account. The results obtained in Lito were compared with those observed in a second apricot cross progeny (Harcot × Reale di Imola) in which QTL of resistance to sharka were also mapped in the same linkage group 1 for both PPV strains. Several models of resistance to sharka disease are discussed considering the segregation frequencies, the QTL alignment in the two maps and the information gathered from the literature.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><doi>10.1007/s11032-010-9431-3</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Apricots Biomedical and Life Sciences Biotechnology breeding Cultivars Disease resistance Gene mapping Genetic control Genetic crosses Life Sciences Molecular biology Plant biology Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Plum pox Plum pox virus Potyviridae Progeny Prunus Prunus armeniaca L QTL analysis Quantitative trait loci Seedlings Sharka Strains (organisms) Viral diseases Viruses |
| title | Identification of QTL for resistance to plum pox virus strains M and D in Lito and Harcot apricot cultivars |
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