Agrobacterium-mediated transformation of gypsophila (Gypsophila paniculata L.)
As a major contributor to the flower market, Gypsophila paniculata is an important target for the breeding of new varieties. However, gypsophila breeding is strongly hampered by the sterility of this species’ genotypes and the lack of a genetic-transformation procedure for this genus. Here we descri...
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| Veröffentlicht in: | Molecular breeding 2008-11, Vol.22 (4), p.543-553 |
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| container_title | Molecular breeding |
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| creator | Moyal Ben Zvi, Michal Zuker, Amir Ovadis, Marianna Shklarman, Elena Ben-Meir, Hagit Zenvirt, Shamir Vainstein, Alexander |
| description | As a major contributor to the flower market,
Gypsophila paniculata
is an important target for the breeding of new varieties. However, gypsophila breeding is strongly hampered by the sterility of this species’ genotypes and the lack of a genetic-transformation procedure for this genus. Here we describe the establishment of a transformation procedure for gypsophila (
Gypsophila paniculata
L.) based on
Agrobacterium
inoculation of highly regenerative stem segments. The transformation procedure employs stem explants derived from GA
3
-pretreated mother plants and a two-step selection scheme. The GA
3
treatment was crucial for obtaining high gene-transfer frequencies (75–90% GUS-expressing explants out of total inoculated explants), as shown using three different gypsophila varieties. An overall transformation efficiency of five GUS-expressing shoots per 100 stem explants was demonstrated for cv. Arbel. The applicability of the transformation system to gypsophila was further reinforced by the generation of transgenic plants expressing
Agrobacterium rhizogenes
rolC
driven by a CaMV 35S promoter. Transgenic gypsophila plantlets exhibited extensive rooting and branching, traits that could be beneficial to the ornamental industry. |
| doi_str_mv | 10.1007/s11032-008-9197-z |
| format | Article |
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Gypsophila paniculata
is an important target for the breeding of new varieties. However, gypsophila breeding is strongly hampered by the sterility of this species’ genotypes and the lack of a genetic-transformation procedure for this genus. Here we describe the establishment of a transformation procedure for gypsophila (
Gypsophila paniculata
L.) based on
Agrobacterium
inoculation of highly regenerative stem segments. The transformation procedure employs stem explants derived from GA
3
-pretreated mother plants and a two-step selection scheme. The GA
3
treatment was crucial for obtaining high gene-transfer frequencies (75–90% GUS-expressing explants out of total inoculated explants), as shown using three different gypsophila varieties. An overall transformation efficiency of five GUS-expressing shoots per 100 stem explants was demonstrated for cv. Arbel. The applicability of the transformation system to gypsophila was further reinforced by the generation of transgenic plants expressing
Agrobacterium rhizogenes
rolC
driven by a CaMV 35S promoter. Transgenic gypsophila plantlets exhibited extensive rooting and branching, traits that could be beneficial to the ornamental industry.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1007/s11032-008-9197-z</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agrobacterium ; Agrobacterium rhizogenes ; Biomedical and Life Sciences ; Biotechnology ; Branching ; Breeding ; Explants ; Genetic transformation ; Genotypes ; Gypsophila ; Gypsophila paniculata ; Inoculation ; Life Sciences ; Molecular biology ; Plant biology ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Plantlets ; Rooting ; Shoots ; Sterility ; Transgenic plants</subject><ispartof>Molecular breeding, 2008-11, Vol.22 (4), p.543-553</ispartof><rights>Springer Science+Business Media B.V. 2008</rights><rights>Molecular Breeding is a copyright of Springer, (2008). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-635fb91e7155d9fe99f1dea47ff1726a47917f856ed217fd9bde90d2b0263a393</citedby><cites>FETCH-LOGICAL-c348t-635fb91e7155d9fe99f1dea47ff1726a47917f856ed217fd9bde90d2b0263a393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11032-008-9197-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11032-008-9197-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Moyal Ben Zvi, Michal</creatorcontrib><creatorcontrib>Zuker, Amir</creatorcontrib><creatorcontrib>Ovadis, Marianna</creatorcontrib><creatorcontrib>Shklarman, Elena</creatorcontrib><creatorcontrib>Ben-Meir, Hagit</creatorcontrib><creatorcontrib>Zenvirt, Shamir</creatorcontrib><creatorcontrib>Vainstein, Alexander</creatorcontrib><title>Agrobacterium-mediated transformation of gypsophila (Gypsophila paniculata L.)</title><title>Molecular breeding</title><addtitle>Mol Breeding</addtitle><description>As a major contributor to the flower market,
Gypsophila paniculata
is an important target for the breeding of new varieties. However, gypsophila breeding is strongly hampered by the sterility of this species’ genotypes and the lack of a genetic-transformation procedure for this genus. Here we describe the establishment of a transformation procedure for gypsophila (
Gypsophila paniculata
L.) based on
Agrobacterium
inoculation of highly regenerative stem segments. The transformation procedure employs stem explants derived from GA
3
-pretreated mother plants and a two-step selection scheme. The GA
3
treatment was crucial for obtaining high gene-transfer frequencies (75–90% GUS-expressing explants out of total inoculated explants), as shown using three different gypsophila varieties. An overall transformation efficiency of five GUS-expressing shoots per 100 stem explants was demonstrated for cv. Arbel. The applicability of the transformation system to gypsophila was further reinforced by the generation of transgenic plants expressing
Agrobacterium rhizogenes
rolC
driven by a CaMV 35S promoter. Transgenic gypsophila plantlets exhibited extensive rooting and branching, traits that could be beneficial to the ornamental industry.</description><subject>Agrobacterium</subject><subject>Agrobacterium rhizogenes</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Branching</subject><subject>Breeding</subject><subject>Explants</subject><subject>Genetic transformation</subject><subject>Genotypes</subject><subject>Gypsophila</subject><subject>Gypsophila paniculata</subject><subject>Inoculation</subject><subject>Life Sciences</subject><subject>Molecular biology</subject><subject>Plant biology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Plantlets</subject><subject>Rooting</subject><subject>Shoots</subject><subject>Sterility</subject><subject>Transgenic plants</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kE9LwzAchoMoOKcfwFvBg3rIzJ82f45DdApDL3oOaZPMjrapSXvYPr0ZFQaCp997eJ6XHy8A1xgtMEL8IWKMKIEICSix5HB_Ama44ARKLsRpylQgSHlOz8FFjFuUHMnYDLwtN8GXuhpsqMcWttbUerAmG4LuovOh1UPtu8y7bLPro--_6kZnd6tj7nVXV2OjB52tF_eX4MzpJtqr3zsHn89PH48vcP2-en1crmFFczFARgtXSmw5LgojnZXSYWN1zp3DnLAUJOZOFMwakoKRpbESGVIiwqimks7B7dTbB_892jioto6VbRrdWT9GJRGhBWNcJPLmD7n1Y-jSc4qQQuZYpMpE4Ymqgo8xWKf6ULc67BRG6jCwmgZWaWB1GFjtk0MmJya229hwbP5f-gElrn5_</recordid><startdate>20081101</startdate><enddate>20081101</enddate><creator>Moyal Ben Zvi, Michal</creator><creator>Zuker, Amir</creator><creator>Ovadis, Marianna</creator><creator>Shklarman, Elena</creator><creator>Ben-Meir, Hagit</creator><creator>Zenvirt, Shamir</creator><creator>Vainstein, Alexander</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20081101</creationdate><title>Agrobacterium-mediated transformation of gypsophila (Gypsophila paniculata L.)</title><author>Moyal Ben Zvi, Michal ; Zuker, Amir ; Ovadis, Marianna ; Shklarman, Elena ; Ben-Meir, Hagit ; Zenvirt, Shamir ; Vainstein, Alexander</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-635fb91e7155d9fe99f1dea47ff1726a47917f856ed217fd9bde90d2b0263a393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Agrobacterium</topic><topic>Agrobacterium rhizogenes</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Branching</topic><topic>Breeding</topic><topic>Explants</topic><topic>Genetic transformation</topic><topic>Genotypes</topic><topic>Gypsophila</topic><topic>Gypsophila paniculata</topic><topic>Inoculation</topic><topic>Life Sciences</topic><topic>Molecular biology</topic><topic>Plant biology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Plantlets</topic><topic>Rooting</topic><topic>Shoots</topic><topic>Sterility</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moyal Ben Zvi, Michal</creatorcontrib><creatorcontrib>Zuker, Amir</creatorcontrib><creatorcontrib>Ovadis, Marianna</creatorcontrib><creatorcontrib>Shklarman, Elena</creatorcontrib><creatorcontrib>Ben-Meir, Hagit</creatorcontrib><creatorcontrib>Zenvirt, Shamir</creatorcontrib><creatorcontrib>Vainstein, Alexander</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moyal Ben Zvi, Michal</au><au>Zuker, Amir</au><au>Ovadis, Marianna</au><au>Shklarman, Elena</au><au>Ben-Meir, Hagit</au><au>Zenvirt, Shamir</au><au>Vainstein, Alexander</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Agrobacterium-mediated transformation of gypsophila (Gypsophila paniculata L.)</atitle><jtitle>Molecular breeding</jtitle><stitle>Mol Breeding</stitle><date>2008-11-01</date><risdate>2008</risdate><volume>22</volume><issue>4</issue><spage>543</spage><epage>553</epage><pages>543-553</pages><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>As a major contributor to the flower market,
Gypsophila paniculata
is an important target for the breeding of new varieties. However, gypsophila breeding is strongly hampered by the sterility of this species’ genotypes and the lack of a genetic-transformation procedure for this genus. Here we describe the establishment of a transformation procedure for gypsophila (
Gypsophila paniculata
L.) based on
Agrobacterium
inoculation of highly regenerative stem segments. The transformation procedure employs stem explants derived from GA
3
-pretreated mother plants and a two-step selection scheme. The GA
3
treatment was crucial for obtaining high gene-transfer frequencies (75–90% GUS-expressing explants out of total inoculated explants), as shown using three different gypsophila varieties. An overall transformation efficiency of five GUS-expressing shoots per 100 stem explants was demonstrated for cv. Arbel. The applicability of the transformation system to gypsophila was further reinforced by the generation of transgenic plants expressing
Agrobacterium rhizogenes
rolC
driven by a CaMV 35S promoter. Transgenic gypsophila plantlets exhibited extensive rooting and branching, traits that could be beneficial to the ornamental industry.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11032-008-9197-z</doi><tpages>11</tpages></addata></record> |
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| ispartof | Molecular breeding, 2008-11, Vol.22 (4), p.543-553 |
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| subjects | Agrobacterium Agrobacterium rhizogenes Biomedical and Life Sciences Biotechnology Branching Breeding Explants Genetic transformation Genotypes Gypsophila Gypsophila paniculata Inoculation Life Sciences Molecular biology Plant biology Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Plantlets Rooting Shoots Sterility Transgenic plants |
| title | Agrobacterium-mediated transformation of gypsophila (Gypsophila paniculata L.) |
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