Molecular Cloning, Sequencing, and Gene Expression Analysis of Tributyltin-Binding Protein Type 1 in Japanese Medaka Fish, Oryzias latipes
The full-length cDNA sequence of tributyltin-binding protein type1 in Japanese medaka (Oryzias latipes) (Olat.TBT-bp1) was determined by means of rapid amplification of cDNA ends (RACE) of liver tissue. Analysis of the structure of the gene encoding Olat.TBT-bp1 revealed that the exonintron organiza...
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| Veröffentlicht in: | Zoological Science 2011-04, Vol.28 (4), p.281-285 |
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| creator | Nassef, Mohamed Kato-Unoki, Yoko Furuta, Tomohisa Nakayama, Kei Satone, Hina Shimasaki, Yohei Honjo, Tsuneo Oshima, Yuji |
| description | The full-length cDNA sequence of tributyltin-binding protein type1 in Japanese medaka (Oryzias latipes) (Olat.TBT-bp1) was determined by means of rapid amplification of cDNA ends (RACE) of liver tissue. Analysis of the structure of the gene encoding Olat.TBT-bp1 revealed that the exonintron organization of this gene corresponds to that of the genes encoding lipocalin superfamily proteins, suggesting that Olat.TBT-bp1 can be categorized as a member of the lipocalin superfamily, which may play an important role in transportation, detoxification, and excretion of xenobiotic compounds. Reverse transcription — PCR revealed that Olat.TBT-bp1 was expressed mainly in the liver, and upregulation of its expression was detected 1, 2, and 4 weeks post hatching. Relative expression of the Olat.TBT-bp1 gene was significantly downregulated, compared with that in the solvent control, by exposure to tributyltin at 0.01 mg/l or triclosan at 1.7 mg/l. Further studies on Olat.TBT-bp1 expression in conjunction with other biochemical and physiological toxicities in response to chemical exposures are needed to increase our understanding and information of TBT-bps mechanisms and as molecular biomarkers of chemical exposures. The role of Olat.TBT-bp1 in xenobiotic detoxification and/or excretion needs more investigations. |
| doi_str_mv | 10.2108/zsj.28.281 |
| format | Article |
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Analysis of the structure of the gene encoding Olat.TBT-bp1 revealed that the exonintron organization of this gene corresponds to that of the genes encoding lipocalin superfamily proteins, suggesting that Olat.TBT-bp1 can be categorized as a member of the lipocalin superfamily, which may play an important role in transportation, detoxification, and excretion of xenobiotic compounds. Reverse transcription — PCR revealed that Olat.TBT-bp1 was expressed mainly in the liver, and upregulation of its expression was detected 1, 2, and 4 weeks post hatching. Relative expression of the Olat.TBT-bp1 gene was significantly downregulated, compared with that in the solvent control, by exposure to tributyltin at 0.01 mg/l or triclosan at 1.7 mg/l. Further studies on Olat.TBT-bp1 expression in conjunction with other biochemical and physiological toxicities in response to chemical exposures are needed to increase our understanding and information of TBT-bps mechanisms and as molecular biomarkers of chemical exposures. The role of Olat.TBT-bp1 in xenobiotic detoxification and/or excretion needs more investigations.</description><identifier>ISSN: 0289-0003</identifier><identifier>DOI: 10.2108/zsj.28.281</identifier><identifier>PMID: 21466346</identifier><language>eng</language><publisher>Japan: UniBio Press</publisher><subject>Animals ; Base Sequence ; Carbamazepine - toxicity ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; cloning ; Cloning, Molecular ; detoxification ; Diclofenac - toxicity ; Fish Proteins - genetics ; Fish Proteins - metabolism ; gene expression ; Gene Expression Regulation ; lipocalin ; Lipocalins - chemistry ; Lipocalins - genetics ; Liver - physiology ; Molecular Sequence Data ; ORIGINAL ARTICLES ; Oryzias - genetics ; Oryzias - metabolism ; Oryzias latipes ; Phylogeny ; Sequence Analysis, DNA ; transportation ; Trialkyltin Compounds - metabolism ; Triclosan - toxicity</subject><ispartof>Zoological Science, 2011-04, Vol.28 (4), p.281-285</ispartof><rights>2011 Zoological Society of Japan</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b443t-d21943e25d46cb4eaedae96d8db0f5ebdbe2f6377604551c1663b6392b9cdc073</citedby><cites>FETCH-LOGICAL-b443t-d21943e25d46cb4eaedae96d8db0f5ebdbe2f6377604551c1663b6392b9cdc073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.2108/zsj.28.281$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>314,780,784,26976,27922,27923,52361</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21466346$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nassef, Mohamed</creatorcontrib><creatorcontrib>Kato-Unoki, Yoko</creatorcontrib><creatorcontrib>Furuta, Tomohisa</creatorcontrib><creatorcontrib>Nakayama, Kei</creatorcontrib><creatorcontrib>Satone, Hina</creatorcontrib><creatorcontrib>Shimasaki, Yohei</creatorcontrib><creatorcontrib>Honjo, Tsuneo</creatorcontrib><creatorcontrib>Oshima, Yuji</creatorcontrib><title>Molecular Cloning, Sequencing, and Gene Expression Analysis of Tributyltin-Binding Protein Type 1 in Japanese Medaka Fish, Oryzias latipes</title><title>Zoological Science</title><addtitle>Zoolog Sci</addtitle><description>The full-length cDNA sequence of tributyltin-binding protein type1 in Japanese medaka (Oryzias latipes) (Olat.TBT-bp1) was determined by means of rapid amplification of cDNA ends (RACE) of liver tissue. Analysis of the structure of the gene encoding Olat.TBT-bp1 revealed that the exonintron organization of this gene corresponds to that of the genes encoding lipocalin superfamily proteins, suggesting that Olat.TBT-bp1 can be categorized as a member of the lipocalin superfamily, which may play an important role in transportation, detoxification, and excretion of xenobiotic compounds. Reverse transcription — PCR revealed that Olat.TBT-bp1 was expressed mainly in the liver, and upregulation of its expression was detected 1, 2, and 4 weeks post hatching. Relative expression of the Olat.TBT-bp1 gene was significantly downregulated, compared with that in the solvent control, by exposure to tributyltin at 0.01 mg/l or triclosan at 1.7 mg/l. Further studies on Olat.TBT-bp1 expression in conjunction with other biochemical and physiological toxicities in response to chemical exposures are needed to increase our understanding and information of TBT-bps mechanisms and as molecular biomarkers of chemical exposures. The role of Olat.TBT-bp1 in xenobiotic detoxification and/or excretion needs more investigations.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Carbamazepine - toxicity</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>cloning</subject><subject>Cloning, Molecular</subject><subject>detoxification</subject><subject>Diclofenac - toxicity</subject><subject>Fish Proteins - genetics</subject><subject>Fish Proteins - metabolism</subject><subject>gene expression</subject><subject>Gene Expression Regulation</subject><subject>lipocalin</subject><subject>Lipocalins - chemistry</subject><subject>Lipocalins - genetics</subject><subject>Liver - physiology</subject><subject>Molecular Sequence Data</subject><subject>ORIGINAL ARTICLES</subject><subject>Oryzias - genetics</subject><subject>Oryzias - metabolism</subject><subject>Oryzias latipes</subject><subject>Phylogeny</subject><subject>Sequence Analysis, DNA</subject><subject>transportation</subject><subject>Trialkyltin Compounds - metabolism</subject><subject>Triclosan - toxicity</subject><issn>0289-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1P3DAQhn0oKh_l0h9Q-dYKEWo7TjY50hVfFYhKLOfIjifF4LVTTyIRfgK_Gi-7RZyQRpqR_M4znncI-crZkeCs-vmE90eiSsE_kR0mqjpjjOXbZBfxnjFe8YJ_JtuCy7LMZblDnq-Cg3Z0KtK5C976v4f0Bv6N4NvXWnlDz8ADPXnsIyDa4OmxV25CizR0dBGtHofJDdZnv6w3qYn-iWEA6-li6oFymqrfqlceEOgVGPWg6KnFu0N6Hacnq5A6Ndge8AvZ6pRD2N_kPXJ7erKYn2eX12cX8-PLTEuZD5kRvJY5iMLIstUSVEJCXZrKaNYVoI0G0ZX5bFYyWRS85WlTXea10HVrWjbL98j3NbePIS2KQ7O02IJz6YthxKZmIi-k5DIpD9bKNgbECF3TR7tUcWo4a1Z2N8nuRlQpeBJ_22BHvQTzJv3v9bu5FuLb8-pGzepGCcIk26B-rJXahuDho6kvy8CX8g</recordid><startdate>201104</startdate><enddate>201104</enddate><creator>Nassef, Mohamed</creator><creator>Kato-Unoki, Yoko</creator><creator>Furuta, Tomohisa</creator><creator>Nakayama, Kei</creator><creator>Satone, Hina</creator><creator>Shimasaki, Yohei</creator><creator>Honjo, Tsuneo</creator><creator>Oshima, Yuji</creator><general>UniBio Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201104</creationdate><title>Molecular Cloning, Sequencing, and Gene Expression Analysis of Tributyltin-Binding Protein Type 1 in Japanese Medaka Fish, Oryzias latipes</title><author>Nassef, Mohamed ; Kato-Unoki, Yoko ; Furuta, Tomohisa ; Nakayama, Kei ; Satone, Hina ; Shimasaki, Yohei ; Honjo, Tsuneo ; Oshima, Yuji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b443t-d21943e25d46cb4eaedae96d8db0f5ebdbe2f6377604551c1663b6392b9cdc073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Carbamazepine - toxicity</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>cloning</topic><topic>Cloning, Molecular</topic><topic>detoxification</topic><topic>Diclofenac - toxicity</topic><topic>Fish Proteins - genetics</topic><topic>Fish Proteins - metabolism</topic><topic>gene expression</topic><topic>Gene Expression Regulation</topic><topic>lipocalin</topic><topic>Lipocalins - chemistry</topic><topic>Lipocalins - genetics</topic><topic>Liver - physiology</topic><topic>Molecular Sequence Data</topic><topic>ORIGINAL ARTICLES</topic><topic>Oryzias - genetics</topic><topic>Oryzias - metabolism</topic><topic>Oryzias latipes</topic><topic>Phylogeny</topic><topic>Sequence Analysis, DNA</topic><topic>transportation</topic><topic>Trialkyltin Compounds - metabolism</topic><topic>Triclosan - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nassef, Mohamed</creatorcontrib><creatorcontrib>Kato-Unoki, Yoko</creatorcontrib><creatorcontrib>Furuta, Tomohisa</creatorcontrib><creatorcontrib>Nakayama, Kei</creatorcontrib><creatorcontrib>Satone, Hina</creatorcontrib><creatorcontrib>Shimasaki, Yohei</creatorcontrib><creatorcontrib>Honjo, Tsuneo</creatorcontrib><creatorcontrib>Oshima, Yuji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Zoological Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nassef, Mohamed</au><au>Kato-Unoki, Yoko</au><au>Furuta, Tomohisa</au><au>Nakayama, Kei</au><au>Satone, Hina</au><au>Shimasaki, Yohei</au><au>Honjo, Tsuneo</au><au>Oshima, Yuji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Cloning, Sequencing, and Gene Expression Analysis of Tributyltin-Binding Protein Type 1 in Japanese Medaka Fish, Oryzias latipes</atitle><jtitle>Zoological Science</jtitle><addtitle>Zoolog Sci</addtitle><date>2011-04</date><risdate>2011</risdate><volume>28</volume><issue>4</issue><spage>281</spage><epage>285</epage><pages>281-285</pages><issn>0289-0003</issn><abstract>The full-length cDNA sequence of tributyltin-binding protein type1 in Japanese medaka (Oryzias latipes) (Olat.TBT-bp1) was determined by means of rapid amplification of cDNA ends (RACE) of liver tissue. Analysis of the structure of the gene encoding Olat.TBT-bp1 revealed that the exonintron organization of this gene corresponds to that of the genes encoding lipocalin superfamily proteins, suggesting that Olat.TBT-bp1 can be categorized as a member of the lipocalin superfamily, which may play an important role in transportation, detoxification, and excretion of xenobiotic compounds. Reverse transcription — PCR revealed that Olat.TBT-bp1 was expressed mainly in the liver, and upregulation of its expression was detected 1, 2, and 4 weeks post hatching. Relative expression of the Olat.TBT-bp1 gene was significantly downregulated, compared with that in the solvent control, by exposure to tributyltin at 0.01 mg/l or triclosan at 1.7 mg/l. Further studies on Olat.TBT-bp1 expression in conjunction with other biochemical and physiological toxicities in response to chemical exposures are needed to increase our understanding and information of TBT-bps mechanisms and as molecular biomarkers of chemical exposures. The role of Olat.TBT-bp1 in xenobiotic detoxification and/or excretion needs more investigations.</abstract><cop>Japan</cop><pub>UniBio Press</pub><pmid>21466346</pmid><doi>10.2108/zsj.28.281</doi><tpages>5</tpages></addata></record> |
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| ispartof | Zoological Science, 2011-04, Vol.28 (4), p.281-285 |
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| language | eng |
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| subjects | Animals Base Sequence Carbamazepine - toxicity Carrier Proteins - genetics Carrier Proteins - metabolism cloning Cloning, Molecular detoxification Diclofenac - toxicity Fish Proteins - genetics Fish Proteins - metabolism gene expression Gene Expression Regulation lipocalin Lipocalins - chemistry Lipocalins - genetics Liver - physiology Molecular Sequence Data ORIGINAL ARTICLES Oryzias - genetics Oryzias - metabolism Oryzias latipes Phylogeny Sequence Analysis, DNA transportation Trialkyltin Compounds - metabolism Triclosan - toxicity |
| title | Molecular Cloning, Sequencing, and Gene Expression Analysis of Tributyltin-Binding Protein Type 1 in Japanese Medaka Fish, Oryzias latipes |
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