Derivation of the King's College London human embryonic stem cell lines
Since the derivation of the first human embryonic stem cell (hESC) line in 1998, there has been substantial interest in the potential of these cells for regenerative medicine and cell therapy and in the use of hESCs carrying clinically relevant genetic mutations as models for disease research and th...
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Veröffentlicht in: | In vitro cellular & developmental biology. Animal 2010-04, Vol.46 (3-4), p.178-185 |
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description | Since the derivation of the first human embryonic stem cell (hESC) line in 1998, there has been substantial interest in the potential of these cells for regenerative medicine and cell therapy and in the use of hESCs carrying clinically relevant genetic mutations as models for disease research and therapeutic target identification. There is still a need to improve derivation efficiency and further the understanding of the basic biology of these cells and to develop clinical grade culture systems with the aim of producing cell lines suitable for subsequent manipulation for therapy. The derivation of initial hESC lines at King's College London is discussed here, with focus on derivation methodology. Each of the derivations was distinctive. Although the stage and morphology of each blastocyst were generally similar in each attempt, the behaviour of the colonies was unpredictable; colony morphology and development was different with each attempt. Days 5, 6 and 7 blastocysts were used successfully, and the number of days until appearance of stem-like cells varied from 4 to 14 d. Routine characterisation analyses were performed on three lines, all of which displayed appropriate marker expression and survived cryopreservation—thaw cycles. From the lines discussed, four are at various stages of the deposition process with the UKSCB, one is pending submission and two are unsuitable for banking. Continued open and transparent reporting of results and collaborations will maximise the efficiency of derivation and facilitate the development of standardised protocols for the derivation and early culture of hESC lines. |
doi_str_mv | 10.1007/s11626-010-9276-4 |
format | Article |
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There is still a need to improve derivation efficiency and further the understanding of the basic biology of these cells and to develop clinical grade culture systems with the aim of producing cell lines suitable for subsequent manipulation for therapy. The derivation of initial hESC lines at King's College London is discussed here, with focus on derivation methodology. Each of the derivations was distinctive. Although the stage and morphology of each blastocyst were generally similar in each attempt, the behaviour of the colonies was unpredictable; colony morphology and development was different with each attempt. Days 5, 6 and 7 blastocysts were used successfully, and the number of days until appearance of stem-like cells varied from 4 to 14 d. Routine characterisation analyses were performed on three lines, all of which displayed appropriate marker expression and survived cryopreservation—thaw cycles. From the lines discussed, four are at various stages of the deposition process with the UKSCB, one is pending submission and two are unsuitable for banking. Continued open and transparent reporting of results and collaborations will maximise the efficiency of derivation and facilitate the development of standardised protocols for the derivation and early culture of hESC lines.</description><identifier>ISSN: 1071-2690</identifier><identifier>EISSN: 1543-706X</identifier><identifier>DOI: 10.1007/s11626-010-9276-4</identifier><identifier>PMID: 20178004</identifier><identifier>CODEN: IVCAED</identifier><language>eng</language><publisher>New York: New York : Springer-Verlag</publisher><subject>Animal Genetics and Genomics ; Animals ; Biomedical and Life Sciences ; Blastocyst ; Cell Biology ; Cell Culture ; Cell culture techniques ; Cell Culture Techniques - methods ; Cell Differentiation ; Cell Line ; Cell lines ; Cell Separation ; Cellular differentiation ; Cultured cells ; Developmental Biology ; Embryo, Mammalian - cytology ; Embryonic stem cells ; Embryonic Stem Cells - cytology ; Embryos ; Freezing ; Humans ; Life Sciences ; London ; Mice ; Neurons - cytology ; Pluripotent stem cells ; Repetitive Sequences, Nucleic Acid - genetics ; Stem Cells ; Stromal cells ; Universities</subject><ispartof>In vitro cellular & developmental biology. Animal, 2010-04, Vol.46 (3-4), p.178-185</ispartof><rights>2010 Society for In Vitro Biology</rights><rights>The Society for In Vitro Biology 2010</rights><rights>Copyright Society for In Vitro Biology Mar/Apr 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-96c117afbdd0534416492c266d2da6317817e0832a9978fee2e17383f81897463</citedby><cites>FETCH-LOGICAL-c448t-96c117afbdd0534416492c266d2da6317817e0832a9978fee2e17383f81897463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/40663794$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/40663794$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,41488,42557,51319,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20178004$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stephenson, Emma L</creatorcontrib><creatorcontrib>Braude, Peter R</creatorcontrib><title>Derivation of the King's College London human embryonic stem cell lines</title><title>In vitro cellular & developmental biology. Animal</title><addtitle>In Vitro Cell.Dev.Biol.-Animal</addtitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><description>Since the derivation of the first human embryonic stem cell (hESC) line in 1998, there has been substantial interest in the potential of these cells for regenerative medicine and cell therapy and in the use of hESCs carrying clinically relevant genetic mutations as models for disease research and therapeutic target identification. There is still a need to improve derivation efficiency and further the understanding of the basic biology of these cells and to develop clinical grade culture systems with the aim of producing cell lines suitable for subsequent manipulation for therapy. The derivation of initial hESC lines at King's College London is discussed here, with focus on derivation methodology. Each of the derivations was distinctive. Although the stage and morphology of each blastocyst were generally similar in each attempt, the behaviour of the colonies was unpredictable; colony morphology and development was different with each attempt. Days 5, 6 and 7 blastocysts were used successfully, and the number of days until appearance of stem-like cells varied from 4 to 14 d. Routine characterisation analyses were performed on three lines, all of which displayed appropriate marker expression and survived cryopreservation—thaw cycles. From the lines discussed, four are at various stages of the deposition process with the UKSCB, one is pending submission and two are unsuitable for banking. Continued open and transparent reporting of results and collaborations will maximise the efficiency of derivation and facilitate the development of standardised protocols for the derivation and early culture of hESC lines.</description><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Blastocyst</subject><subject>Cell Biology</subject><subject>Cell Culture</subject><subject>Cell culture techniques</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cell Separation</subject><subject>Cellular differentiation</subject><subject>Cultured cells</subject><subject>Developmental Biology</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryonic stem cells</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryos</subject><subject>Freezing</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>London</subject><subject>Mice</subject><subject>Neurons - cytology</subject><subject>Pluripotent stem cells</subject><subject>Repetitive Sequences, Nucleic Acid - genetics</subject><subject>Stem Cells</subject><subject>Stromal cells</subject><subject>Universities</subject><issn>1071-2690</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFUctu1DAUtRCIloEPYAFYbLoK3Gs7fiyrAVrESCygEjvLkzjTjBK72Eml_j2OUorEAryxpfO41-cQ8hLhHQKo9xlRMlkBQmWYkpV4RE6xFrxSIH88Lm9QWDFp4IQ8y_kI5RiUT8kJA1QaQJySiw8-9bdu6mOgsaPTtadf-nA4y3Qbh8EfPN3F0Bbweh5doH7cp7sY-obmyY-08cNAhz74_Jw86dyQ_Yv7e0OuPn38vr2sdl8vPm_Pd1UjhJ4qIxtE5bp920LNhUApDGuYlC1rneRlK1QeNGfOGKU775lHxTXvNGqjhOQbcrb63qT4c_Z5smOflzVc8HHO1gDjNZf6_0zFeW1QKCjMt38xj3FOoXzDCmM0wyXTDcGV1KSYc_KdvUn96NKdRbBLG3Ztw5Y27NKGXTSv743n_ejbB8Xv-AuBrYRcoHDw6c_kf7m-WkXHPMX0YCpASq7Mgr9Z8c5F6w6pz_bqWxnJAUuwHGv-C45KpCQ</recordid><startdate>20100401</startdate><enddate>20100401</enddate><creator>Stephenson, Emma L</creator><creator>Braude, Peter R</creator><general>New York : Springer-Verlag</general><general>Springer Science + Business Media</general><general>Springer-Verlag</general><general>Society for In Vitro Biology</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope><scope>7QO</scope><scope>RC3</scope></search><sort><creationdate>20100401</creationdate><title>Derivation of the King's College London human embryonic stem cell lines</title><author>Stephenson, Emma L ; Braude, Peter R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-96c117afbdd0534416492c266d2da6317817e0832a9978fee2e17383f81897463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Blastocyst</topic><topic>Cell Biology</topic><topic>Cell Culture</topic><topic>Cell culture techniques</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cell Separation</topic><topic>Cellular differentiation</topic><topic>Cultured cells</topic><topic>Developmental Biology</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryonic stem cells</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryos</topic><topic>Freezing</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>London</topic><topic>Mice</topic><topic>Neurons - cytology</topic><topic>Pluripotent stem cells</topic><topic>Repetitive Sequences, Nucleic Acid - genetics</topic><topic>Stem Cells</topic><topic>Stromal cells</topic><topic>Universities</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stephenson, Emma L</creatorcontrib><creatorcontrib>Braude, Peter R</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>In vitro cellular & developmental biology. Animal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stephenson, Emma L</au><au>Braude, Peter R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Derivation of the King's College London human embryonic stem cell lines</atitle><jtitle>In vitro cellular & developmental biology. Animal</jtitle><stitle>In Vitro Cell.Dev.Biol.-Animal</stitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><date>2010-04-01</date><risdate>2010</risdate><volume>46</volume><issue>3-4</issue><spage>178</spage><epage>185</epage><pages>178-185</pages><issn>1071-2690</issn><eissn>1543-706X</eissn><coden>IVCAED</coden><abstract>Since the derivation of the first human embryonic stem cell (hESC) line in 1998, there has been substantial interest in the potential of these cells for regenerative medicine and cell therapy and in the use of hESCs carrying clinically relevant genetic mutations as models for disease research and therapeutic target identification. There is still a need to improve derivation efficiency and further the understanding of the basic biology of these cells and to develop clinical grade culture systems with the aim of producing cell lines suitable for subsequent manipulation for therapy. The derivation of initial hESC lines at King's College London is discussed here, with focus on derivation methodology. Each of the derivations was distinctive. Although the stage and morphology of each blastocyst were generally similar in each attempt, the behaviour of the colonies was unpredictable; colony morphology and development was different with each attempt. Days 5, 6 and 7 blastocysts were used successfully, and the number of days until appearance of stem-like cells varied from 4 to 14 d. Routine characterisation analyses were performed on three lines, all of which displayed appropriate marker expression and survived cryopreservation—thaw cycles. From the lines discussed, four are at various stages of the deposition process with the UKSCB, one is pending submission and two are unsuitable for banking. Continued open and transparent reporting of results and collaborations will maximise the efficiency of derivation and facilitate the development of standardised protocols for the derivation and early culture of hESC lines.</abstract><cop>New York</cop><pub>New York : Springer-Verlag</pub><pmid>20178004</pmid><doi>10.1007/s11626-010-9276-4</doi><tpages>8</tpages></addata></record> |
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subjects | Animal Genetics and Genomics Animals Biomedical and Life Sciences Blastocyst Cell Biology Cell Culture Cell culture techniques Cell Culture Techniques - methods Cell Differentiation Cell Line Cell lines Cell Separation Cellular differentiation Cultured cells Developmental Biology Embryo, Mammalian - cytology Embryonic stem cells Embryonic Stem Cells - cytology Embryos Freezing Humans Life Sciences London Mice Neurons - cytology Pluripotent stem cells Repetitive Sequences, Nucleic Acid - genetics Stem Cells Stromal cells Universities |
title | Derivation of the King's College London human embryonic stem cell lines |
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