A novel DNA-based diagnostic test for the detection of annual and intermediate ryegrass contamination in perennial ryegrass
Perennial ryegrass ( Lolium perenne L.) is a preferred choice for the turf grass industry due to its ability to provide a durable turf cover. Genetic or physical contamination of annual ( L. multiflorum Lam.) or intermediate ( L. hybridum ) ryegrass species in perennial ryegrass is one of the major...
Gespeichert in:
| Veröffentlicht in: | Molecular breeding 2011-08, Vol.28 (2), p.217-225 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 225 |
|---|---|
| container_issue | 2 |
| container_start_page | 217 |
| container_title | Molecular breeding |
| container_volume | 28 |
| creator | Chandra-Shekara, A. C. Pegadaraju, Venkatramana Thompson, Michael Vellekson, Donn Schultz, Quentin |
| description | Perennial ryegrass (
Lolium perenne
L.) is a preferred choice for the turf grass industry due to its ability to provide a durable turf cover. Genetic or physical contamination of annual (
L. multiflorum
Lam.) or intermediate (
L. hybridum
) ryegrass species in perennial ryegrass is one of the major problems affecting the grass seed industry. At present, seedling root fluorescence (SRF), a biochemical marker, is used for the detection of annual ryegrass contamination. Due to the unreliability of the SRF test, the seed industry is seeking an alternative, more reliable and accurate detection method. Currently, there are no DNA tests available in ryegrass for detecting contamination with annual and intermediate ryegrass types. We developed a novel quantitative polymerase chain reaction (Q-PCR)-based DNA test for the detection of annual and/or intermediate ryegrass types in perennial ryegrass. This DNA test was designed using an insertion/deletion (InDel) site in the
LpVRN2_2
(
Vernalization 2
) gene, which is one of the several genes controlling vernalization in ryegrass. The new DNA test is more reliable, accurate and cost-effective in detecting contamination, with a high sensitivity of 0.04% in a sample size of 5,000 seeds. Use of larger sample sizes (12.5-fold higher compared to SRF test) provided additional accuracy in detecting the level of contamination. The method has produced consistent results in 68 perennial, 26 annual and 14 intermediate ryegrass lines. |
| doi_str_mv | 10.1007/s11032-010-9475-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_902353421</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>902353421</sourcerecordid><originalsourceid>FETCH-LOGICAL-c348t-15fd7a28d4e4461b9ba5400f6a8326bc3199bf1fe03c47e44fa230510c1d69e3</originalsourceid><addsrcrecordid>eNp1kctKQzEQhg-i4PUB3AVcuIrmdi5ZlnqFopvuQ07OpKacJjVJBfHlTa0iCK5mFt_3M8NfVeeUXFFC2utEKeEME0qwFG2NxV51ROuWYdl23X7ZeUcwbwU_rI5TWpLiyKY5qj4myIc3GNHN0wT3OsGABqcXPqTsDMqQMrIhovwCaIAMJrvgUbBIe7_RYxkDcj5DXEHRMqD4DouoU0Im-KxXzusvw3m0hgjeuyL9MKfVgdVjgrPveVLN727n0wc8e75_nE5m2HDRZUxrO7SadYMAIRray17XghDb6I6zpjecStlbaoFwI9rCWM04qSkxdGgk8JPqche7juF1Uz5SK5cMjKP2EDZJScJ4zQWjhbz4Qy7DJvpym2KsloLQmvNC0R1lYkgpglXr6FY6vitK1LYMtStDlTLUtgwlisN2TiqsX0D8Tf5f-gTY9Y2C</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2259401533</pqid></control><display><type>article</type><title>A novel DNA-based diagnostic test for the detection of annual and intermediate ryegrass contamination in perennial ryegrass</title><source>SpringerLink Journals - AutoHoldings</source><creator>Chandra-Shekara, A. C. ; Pegadaraju, Venkatramana ; Thompson, Michael ; Vellekson, Donn ; Schultz, Quentin</creator><creatorcontrib>Chandra-Shekara, A. C. ; Pegadaraju, Venkatramana ; Thompson, Michael ; Vellekson, Donn ; Schultz, Quentin</creatorcontrib><description>Perennial ryegrass (
Lolium perenne
L.) is a preferred choice for the turf grass industry due to its ability to provide a durable turf cover. Genetic or physical contamination of annual (
L. multiflorum
Lam.) or intermediate (
L. hybridum
) ryegrass species in perennial ryegrass is one of the major problems affecting the grass seed industry. At present, seedling root fluorescence (SRF), a biochemical marker, is used for the detection of annual ryegrass contamination. Due to the unreliability of the SRF test, the seed industry is seeking an alternative, more reliable and accurate detection method. Currently, there are no DNA tests available in ryegrass for detecting contamination with annual and intermediate ryegrass types. We developed a novel quantitative polymerase chain reaction (Q-PCR)-based DNA test for the detection of annual and/or intermediate ryegrass types in perennial ryegrass. This DNA test was designed using an insertion/deletion (InDel) site in the
LpVRN2_2
(
Vernalization 2
) gene, which is one of the several genes controlling vernalization in ryegrass. The new DNA test is more reliable, accurate and cost-effective in detecting contamination, with a high sensitivity of 0.04% in a sample size of 5,000 seeds. Use of larger sample sizes (12.5-fold higher compared to SRF test) provided additional accuracy in detecting the level of contamination. The method has produced consistent results in 68 perennial, 26 annual and 14 intermediate ryegrass lines.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1007/s11032-010-9475-4</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biochemical markers ; Biomedical and Life Sciences ; Biotechnology ; Contamination ; Deoxyribonucleic acid ; Diagnostic systems ; Diagnostic tests ; DNA ; Fluorescence ; Gene deletion ; Genetic testing ; Grasses ; Insertion ; Lawns ; Life Sciences ; Lolium perenne ; Molecular biology ; Plant biology ; Plant Genetics and Genomics ; Plant growth ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Polymerase chain reaction ; Seedlings ; Seeds ; Turf ; Vernalization</subject><ispartof>Molecular breeding, 2011-08, Vol.28 (2), p.217-225</ispartof><rights>Springer Science+Business Media B.V. 2010</rights><rights>Molecular Breeding is a copyright of Springer, (2010). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-15fd7a28d4e4461b9ba5400f6a8326bc3199bf1fe03c47e44fa230510c1d69e3</citedby><cites>FETCH-LOGICAL-c348t-15fd7a28d4e4461b9ba5400f6a8326bc3199bf1fe03c47e44fa230510c1d69e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11032-010-9475-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11032-010-9475-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Chandra-Shekara, A. C.</creatorcontrib><creatorcontrib>Pegadaraju, Venkatramana</creatorcontrib><creatorcontrib>Thompson, Michael</creatorcontrib><creatorcontrib>Vellekson, Donn</creatorcontrib><creatorcontrib>Schultz, Quentin</creatorcontrib><title>A novel DNA-based diagnostic test for the detection of annual and intermediate ryegrass contamination in perennial ryegrass</title><title>Molecular breeding</title><addtitle>Mol Breeding</addtitle><description>Perennial ryegrass (
Lolium perenne
L.) is a preferred choice for the turf grass industry due to its ability to provide a durable turf cover. Genetic or physical contamination of annual (
L. multiflorum
Lam.) or intermediate (
L. hybridum
) ryegrass species in perennial ryegrass is one of the major problems affecting the grass seed industry. At present, seedling root fluorescence (SRF), a biochemical marker, is used for the detection of annual ryegrass contamination. Due to the unreliability of the SRF test, the seed industry is seeking an alternative, more reliable and accurate detection method. Currently, there are no DNA tests available in ryegrass for detecting contamination with annual and intermediate ryegrass types. We developed a novel quantitative polymerase chain reaction (Q-PCR)-based DNA test for the detection of annual and/or intermediate ryegrass types in perennial ryegrass. This DNA test was designed using an insertion/deletion (InDel) site in the
LpVRN2_2
(
Vernalization 2
) gene, which is one of the several genes controlling vernalization in ryegrass. The new DNA test is more reliable, accurate and cost-effective in detecting contamination, with a high sensitivity of 0.04% in a sample size of 5,000 seeds. Use of larger sample sizes (12.5-fold higher compared to SRF test) provided additional accuracy in detecting the level of contamination. The method has produced consistent results in 68 perennial, 26 annual and 14 intermediate ryegrass lines.</description><subject>Biochemical markers</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Contamination</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnostic systems</subject><subject>Diagnostic tests</subject><subject>DNA</subject><subject>Fluorescence</subject><subject>Gene deletion</subject><subject>Genetic testing</subject><subject>Grasses</subject><subject>Insertion</subject><subject>Lawns</subject><subject>Life Sciences</subject><subject>Lolium perenne</subject><subject>Molecular biology</subject><subject>Plant biology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant growth</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Polymerase chain reaction</subject><subject>Seedlings</subject><subject>Seeds</subject><subject>Turf</subject><subject>Vernalization</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kctKQzEQhg-i4PUB3AVcuIrmdi5ZlnqFopvuQ07OpKacJjVJBfHlTa0iCK5mFt_3M8NfVeeUXFFC2utEKeEME0qwFG2NxV51ROuWYdl23X7ZeUcwbwU_rI5TWpLiyKY5qj4myIc3GNHN0wT3OsGABqcXPqTsDMqQMrIhovwCaIAMJrvgUbBIe7_RYxkDcj5DXEHRMqD4DouoU0Im-KxXzusvw3m0hgjeuyL9MKfVgdVjgrPveVLN727n0wc8e75_nE5m2HDRZUxrO7SadYMAIRray17XghDb6I6zpjecStlbaoFwI9rCWM04qSkxdGgk8JPqche7juF1Uz5SK5cMjKP2EDZJScJ4zQWjhbz4Qy7DJvpym2KsloLQmvNC0R1lYkgpglXr6FY6vitK1LYMtStDlTLUtgwlisN2TiqsX0D8Tf5f-gTY9Y2C</recordid><startdate>20110801</startdate><enddate>20110801</enddate><creator>Chandra-Shekara, A. C.</creator><creator>Pegadaraju, Venkatramana</creator><creator>Thompson, Michael</creator><creator>Vellekson, Donn</creator><creator>Schultz, Quentin</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20110801</creationdate><title>A novel DNA-based diagnostic test for the detection of annual and intermediate ryegrass contamination in perennial ryegrass</title><author>Chandra-Shekara, A. C. ; Pegadaraju, Venkatramana ; Thompson, Michael ; Vellekson, Donn ; Schultz, Quentin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-15fd7a28d4e4461b9ba5400f6a8326bc3199bf1fe03c47e44fa230510c1d69e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biochemical markers</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Contamination</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnostic systems</topic><topic>Diagnostic tests</topic><topic>DNA</topic><topic>Fluorescence</topic><topic>Gene deletion</topic><topic>Genetic testing</topic><topic>Grasses</topic><topic>Insertion</topic><topic>Lawns</topic><topic>Life Sciences</topic><topic>Lolium perenne</topic><topic>Molecular biology</topic><topic>Plant biology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant growth</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Polymerase chain reaction</topic><topic>Seedlings</topic><topic>Seeds</topic><topic>Turf</topic><topic>Vernalization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chandra-Shekara, A. C.</creatorcontrib><creatorcontrib>Pegadaraju, Venkatramana</creatorcontrib><creatorcontrib>Thompson, Michael</creatorcontrib><creatorcontrib>Vellekson, Donn</creatorcontrib><creatorcontrib>Schultz, Quentin</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chandra-Shekara, A. C.</au><au>Pegadaraju, Venkatramana</au><au>Thompson, Michael</au><au>Vellekson, Donn</au><au>Schultz, Quentin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel DNA-based diagnostic test for the detection of annual and intermediate ryegrass contamination in perennial ryegrass</atitle><jtitle>Molecular breeding</jtitle><stitle>Mol Breeding</stitle><date>2011-08-01</date><risdate>2011</risdate><volume>28</volume><issue>2</issue><spage>217</spage><epage>225</epage><pages>217-225</pages><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>Perennial ryegrass (
Lolium perenne
L.) is a preferred choice for the turf grass industry due to its ability to provide a durable turf cover. Genetic or physical contamination of annual (
L. multiflorum
Lam.) or intermediate (
L. hybridum
) ryegrass species in perennial ryegrass is one of the major problems affecting the grass seed industry. At present, seedling root fluorescence (SRF), a biochemical marker, is used for the detection of annual ryegrass contamination. Due to the unreliability of the SRF test, the seed industry is seeking an alternative, more reliable and accurate detection method. Currently, there are no DNA tests available in ryegrass for detecting contamination with annual and intermediate ryegrass types. We developed a novel quantitative polymerase chain reaction (Q-PCR)-based DNA test for the detection of annual and/or intermediate ryegrass types in perennial ryegrass. This DNA test was designed using an insertion/deletion (InDel) site in the
LpVRN2_2
(
Vernalization 2
) gene, which is one of the several genes controlling vernalization in ryegrass. The new DNA test is more reliable, accurate and cost-effective in detecting contamination, with a high sensitivity of 0.04% in a sample size of 5,000 seeds. Use of larger sample sizes (12.5-fold higher compared to SRF test) provided additional accuracy in detecting the level of contamination. The method has produced consistent results in 68 perennial, 26 annual and 14 intermediate ryegrass lines.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11032-010-9475-4</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1380-3743 |
| ispartof | Molecular breeding, 2011-08, Vol.28 (2), p.217-225 |
| issn | 1380-3743 1572-9788 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_902353421 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Biochemical markers Biomedical and Life Sciences Biotechnology Contamination Deoxyribonucleic acid Diagnostic systems Diagnostic tests DNA Fluorescence Gene deletion Genetic testing Grasses Insertion Lawns Life Sciences Lolium perenne Molecular biology Plant biology Plant Genetics and Genomics Plant growth Plant Pathology Plant Physiology Plant Sciences Polymerase chain reaction Seedlings Seeds Turf Vernalization |
| title | A novel DNA-based diagnostic test for the detection of annual and intermediate ryegrass contamination in perennial ryegrass |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T02%3A14%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20novel%20DNA-based%20diagnostic%20test%20for%20the%20detection%20of%20annual%20and%20intermediate%20ryegrass%20contamination%20in%20perennial%20ryegrass&rft.jtitle=Molecular%20breeding&rft.au=Chandra-Shekara,%20A.%20C.&rft.date=2011-08-01&rft.volume=28&rft.issue=2&rft.spage=217&rft.epage=225&rft.pages=217-225&rft.issn=1380-3743&rft.eissn=1572-9788&rft_id=info:doi/10.1007/s11032-010-9475-4&rft_dat=%3Cproquest_cross%3E902353421%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2259401533&rft_id=info:pmid/&rfr_iscdi=true |