Identification and control of bacterial contaminants from Ilex dumosa nodal segments culture in a temporal immersion bioreactor system using 16S rDNA analysis
Endogenous bacterial contaminants isolated from infected cultures of Ilex dumosa nodal segments were identified as Stenotrophomonas maltophilia and Achromobacter sp. using 16S rDNA analysis. A range of antibiotics with different mechanism of actions and the commercial biocide PPM[trade mark sign] we...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2008-10, Vol.95 (1), p.13-19 |
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| container_title | Plant cell, tissue and organ culture |
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| creator | Luna, Claudia Collavino, Mónica Mroginski, Luis Sansberro, Pedro |
| description | Endogenous bacterial contaminants isolated from infected cultures of Ilex dumosa nodal segments were identified as Stenotrophomonas maltophilia and Achromobacter sp. using 16S rDNA analysis. A range of antibiotics with different mechanism of actions and the commercial biocide PPM[trade mark sign] were tested for their capacity to repress the growth of Gram negative bacteria grown in liquid medium during the establishment phase of temporal immersion systems. The best results were obtained with the addition of 0.5 mg ml⁻¹ cefotaxime to the culture media obtaining 100% of uncontaminated cultures without suppress of shoot growth. |
| doi_str_mv | 10.1007/s11240-008-9408-7 |
| format | Article |
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A range of antibiotics with different mechanism of actions and the commercial biocide PPM[trade mark sign] were tested for their capacity to repress the growth of Gram negative bacteria grown in liquid medium during the establishment phase of temporal immersion systems. The best results were obtained with the addition of 0.5 mg ml⁻¹ cefotaxime to the culture media obtaining 100% of uncontaminated cultures without suppress of shoot growth.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-008-9408-7</identifier><identifier>CODEN: PTCEDJ</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Achromobacter ; antibiotics ; Bacteria ; bacterial contamination ; Biological and medical sciences ; Biomedical and Life Sciences ; Bioreactors ; Biotechnology ; cefotaxime ; culture media ; Fundamental and applied biological sciences. Psychology ; Gram-negative bacteria ; Ilex ; Ilex dumosa ; Life Sciences ; mechanism of action ; medicinal plants ; Methods. Procedures. Technologies ; micropropagation ; Original Paper ; pathogen identification ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; ribosomal DNA ; Stenotrophomonas maltophilia ; Various methods and equipments</subject><ispartof>Plant cell, tissue and organ culture, 2008-10, Vol.95 (1), p.13-19</ispartof><rights>Springer Science+Business Media B.V. 2008</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-57a6ae7339f280c0e430dad325422289420267b6da90f127fd3eff9db90f098e3</citedby><cites>FETCH-LOGICAL-c374t-57a6ae7339f280c0e430dad325422289420267b6da90f127fd3eff9db90f098e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-008-9408-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-008-9408-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20629407$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Luna, Claudia</creatorcontrib><creatorcontrib>Collavino, Mónica</creatorcontrib><creatorcontrib>Mroginski, Luis</creatorcontrib><creatorcontrib>Sansberro, Pedro</creatorcontrib><title>Identification and control of bacterial contaminants from Ilex dumosa nodal segments culture in a temporal immersion bioreactor system using 16S rDNA analysis</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>Endogenous bacterial contaminants isolated from infected cultures of Ilex dumosa nodal segments were identified as Stenotrophomonas maltophilia and Achromobacter sp. using 16S rDNA analysis. A range of antibiotics with different mechanism of actions and the commercial biocide PPM[trade mark sign] were tested for their capacity to repress the growth of Gram negative bacteria grown in liquid medium during the establishment phase of temporal immersion systems. The best results were obtained with the addition of 0.5 mg ml⁻¹ cefotaxime to the culture media obtaining 100% of uncontaminated cultures without suppress of shoot growth.</description><subject>Achromobacter</subject><subject>antibiotics</subject><subject>Bacteria</subject><subject>bacterial contamination</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>cefotaxime</subject><subject>culture media</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gram-negative bacteria</subject><subject>Ilex</subject><subject>Ilex dumosa</subject><subject>Life Sciences</subject><subject>mechanism of action</subject><subject>medicinal plants</subject><subject>Methods. Procedures. Technologies</subject><subject>micropropagation</subject><subject>Original Paper</subject><subject>pathogen identification</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>ribosomal DNA</subject><subject>Stenotrophomonas maltophilia</subject><subject>Various methods and equipments</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp9kUtv1DAUhSMEEkPbH9AV3iBWgetH4mRZlddIFSxK19Ydxx65SuzBN5GYP9PfikMqlmz8Ot85tnyq6prDBw6gPxLnQkEN0NW9KoN-Ue14o2XdgFIvqx3wVtdt1-jX1RuiRwBopeK76mk_uDgHHyzOIUWGcWA2xTmnkSXPDmhnlwOOfw9xChHjTMznNLH96H6zYZkSIYtpKAy54-RW3S7jvGTHQglks5tOKRc5TJPLtN5yCCm7Ep0yozMVgC0U4pHx9p7lT99vyjNwPFOgy-qVx5Hc1fN8UT18-fzz9lt99-Pr_vbmrrZSq7luNLbotJS9Fx1YcErCgIMUjRJCdL0SIFp9aAfswXOh_SCd9_1wKFvoOycvqvdb7imnX4uj2UyBrBtHjC4tZHoQsoGul4XkG2lzIsrOm1MOE-az4WDWKsxWhSlVmLUKo4vn3XM6ksXRZ4w20D-jgFYUcuXExlGR4tFl85iWXL6C_hv-djN5TAaPuQQ_3AvgEqAXnSqLPxX6pPM</recordid><startdate>20081001</startdate><enddate>20081001</enddate><creator>Luna, Claudia</creator><creator>Collavino, Mónica</creator><creator>Mroginski, Luis</creator><creator>Sansberro, Pedro</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20081001</creationdate><title>Identification and control of bacterial contaminants from Ilex dumosa nodal segments culture in a temporal immersion bioreactor system using 16S rDNA analysis</title><author>Luna, Claudia ; Collavino, Mónica ; Mroginski, Luis ; Sansberro, Pedro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-57a6ae7339f280c0e430dad325422289420267b6da90f127fd3eff9db90f098e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Achromobacter</topic><topic>antibiotics</topic><topic>Bacteria</topic><topic>bacterial contamination</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Bioreactors</topic><topic>Biotechnology</topic><topic>cefotaxime</topic><topic>culture media</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gram-negative bacteria</topic><topic>Ilex</topic><topic>Ilex dumosa</topic><topic>Life Sciences</topic><topic>mechanism of action</topic><topic>medicinal plants</topic><topic>Methods. Procedures. Technologies</topic><topic>micropropagation</topic><topic>Original Paper</topic><topic>pathogen identification</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>ribosomal DNA</topic><topic>Stenotrophomonas maltophilia</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luna, Claudia</creatorcontrib><creatorcontrib>Collavino, Mónica</creatorcontrib><creatorcontrib>Mroginski, Luis</creatorcontrib><creatorcontrib>Sansberro, Pedro</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luna, Claudia</au><au>Collavino, Mónica</au><au>Mroginski, Luis</au><au>Sansberro, Pedro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and control of bacterial contaminants from Ilex dumosa nodal segments culture in a temporal immersion bioreactor system using 16S rDNA analysis</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2008-10-01</date><risdate>2008</risdate><volume>95</volume><issue>1</issue><spage>13</spage><epage>19</epage><pages>13-19</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><coden>PTCEDJ</coden><abstract>Endogenous bacterial contaminants isolated from infected cultures of Ilex dumosa nodal segments were identified as Stenotrophomonas maltophilia and Achromobacter sp. using 16S rDNA analysis. 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| fulltext | fulltext |
| identifier | ISSN: 0167-6857 |
| ispartof | Plant cell, tissue and organ culture, 2008-10, Vol.95 (1), p.13-19 |
| issn | 0167-6857 1573-5044 |
| language | eng |
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| subjects | Achromobacter antibiotics Bacteria bacterial contamination Biological and medical sciences Biomedical and Life Sciences Bioreactors Biotechnology cefotaxime culture media Fundamental and applied biological sciences. Psychology Gram-negative bacteria Ilex Ilex dumosa Life Sciences mechanism of action medicinal plants Methods. Procedures. Technologies micropropagation Original Paper pathogen identification Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences ribosomal DNA Stenotrophomonas maltophilia Various methods and equipments |
| title | Identification and control of bacterial contaminants from Ilex dumosa nodal segments culture in a temporal immersion bioreactor system using 16S rDNA analysis |
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