effect of cytokinins on growth and sexual organ development in the gametophyte of Blechnum spicant L
In this study, we report the role of exogenous and endogenous cytokinins on growth and sexual organ development in the fern Blechnum spicant L. Spore-derived gametophytes (SG) were cultured in full-strength Murashige and Skoog (1962) liquid medium supplemented with (a) 4.44 μMN⁶-benzyladenine (BAP),...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2009-03, Vol.96 (3), p.245-250 |
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| creator | Menéndez, V Revilla, M. A Fal, M. A Fernández, H |
| description | In this study, we report the role of exogenous and endogenous cytokinins on growth and sexual organ development in the fern Blechnum spicant L. Spore-derived gametophytes (SG) were cultured in full-strength Murashige and Skoog (1962) liquid medium supplemented with (a) 4.44 μMN⁶-benzyladenine (BAP), (b) a crude extract from mature female gametophytes, and (c) 4.44 μM BAP in combination with the crude extract from mature gametophytes, respectively. Both BAP and the crude extract delayed the gametophyte development, and this effect was increased when they were added together. With respect to sexual organ development, BAP inhibited the sexual organ formation, while the crude extract favored antheridia formation; however, when added together, the percentage of antheridia decreased. The endogenous level of the cytokinins cis-zeatin (cZ), cis-zeatin-riboside (cZR), dihydrozeatin (DHZ), dihydrozeatin riboside (DHZR), isopentenyl adenine (iP), isopentenyl adenosine (iPR), isopentenyl-9-glucoside (iP9G), trans-zeatin (tZ), and trans-zeatin riboside (tZR) were analyzed in female and male gametophytes of B. spicant L. The endogenous levels of cytokinins tZ, cZ, DHZ, cZR, iP, and iPR were higher in female gametophytes than in male gametophytes, with the endogenous iP and iPR content being increased more than 300 and 400 times, respectively. |
| doi_str_mv | 10.1007/s11240-008-9481-y |
| format | Article |
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A ; Fal, M. A ; Fernández, H</creator><creatorcontrib>Menéndez, V ; Revilla, M. A ; Fal, M. A ; Fernández, H</creatorcontrib><description>In this study, we report the role of exogenous and endogenous cytokinins on growth and sexual organ development in the fern Blechnum spicant L. Spore-derived gametophytes (SG) were cultured in full-strength Murashige and Skoog (1962) liquid medium supplemented with (a) 4.44 μMN⁶-benzyladenine (BAP), (b) a crude extract from mature female gametophytes, and (c) 4.44 μM BAP in combination with the crude extract from mature gametophytes, respectively. Both BAP and the crude extract delayed the gametophyte development, and this effect was increased when they were added together. With respect to sexual organ development, BAP inhibited the sexual organ formation, while the crude extract favored antheridia formation; however, when added together, the percentage of antheridia decreased. The endogenous level of the cytokinins cis-zeatin (cZ), cis-zeatin-riboside (cZR), dihydrozeatin (DHZ), dihydrozeatin riboside (DHZR), isopentenyl adenine (iP), isopentenyl adenosine (iPR), isopentenyl-9-glucoside (iP9G), trans-zeatin (tZ), and trans-zeatin riboside (tZR) were analyzed in female and male gametophytes of B. spicant L. The endogenous levels of cytokinins tZ, cZ, DHZ, cZR, iP, and iPR were higher in female gametophytes than in male gametophytes, with the endogenous iP and iPR content being increased more than 300 and 400 times, respectively.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-008-9481-y</identifier><identifier>CODEN: PTCEDJ</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Adenine ; Adenosine ; Benzyladenine ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Blechnum spicant ; culture media ; Cytokinins ; Ferns ; ferns and fern allies ; Fundamental and applied biological sciences. Psychology ; Gametophytes ; germ cells ; isopentenyl adenosine ; isopentenyl-9-glucoside ; isopentenyladenine ; Life Sciences ; organogenesis ; Original Paper ; plant extracts ; Plant Genetics and Genomics ; plant hormones ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Zeatin ; Zeatin riboside</subject><ispartof>Plant cell, tissue and organ culture, 2009-03, Vol.96 (3), p.245-250</ispartof><rights>Springer Science+Business Media B.V. 2008</rights><rights>2009 INIST-CNRS</rights><rights>Plant Cell, Tissue and Organ Culture (PCTOC) is a copyright of Springer, (2008). 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A</creatorcontrib><creatorcontrib>Fal, M. A</creatorcontrib><creatorcontrib>Fernández, H</creatorcontrib><title>effect of cytokinins on growth and sexual organ development in the gametophyte of Blechnum spicant L</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>In this study, we report the role of exogenous and endogenous cytokinins on growth and sexual organ development in the fern Blechnum spicant L. Spore-derived gametophytes (SG) were cultured in full-strength Murashige and Skoog (1962) liquid medium supplemented with (a) 4.44 μMN⁶-benzyladenine (BAP), (b) a crude extract from mature female gametophytes, and (c) 4.44 μM BAP in combination with the crude extract from mature gametophytes, respectively. Both BAP and the crude extract delayed the gametophyte development, and this effect was increased when they were added together. With respect to sexual organ development, BAP inhibited the sexual organ formation, while the crude extract favored antheridia formation; however, when added together, the percentage of antheridia decreased. The endogenous level of the cytokinins cis-zeatin (cZ), cis-zeatin-riboside (cZR), dihydrozeatin (DHZ), dihydrozeatin riboside (DHZR), isopentenyl adenine (iP), isopentenyl adenosine (iPR), isopentenyl-9-glucoside (iP9G), trans-zeatin (tZ), and trans-zeatin riboside (tZR) were analyzed in female and male gametophytes of B. spicant L. The endogenous levels of cytokinins tZ, cZ, DHZ, cZR, iP, and iPR were higher in female gametophytes than in male gametophytes, with the endogenous iP and iPR content being increased more than 300 and 400 times, respectively.</description><subject>Adenine</subject><subject>Adenosine</subject><subject>Benzyladenine</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Blechnum spicant</subject><subject>culture media</subject><subject>Cytokinins</subject><subject>Ferns</subject><subject>ferns and fern allies</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gametophytes</subject><subject>germ cells</subject><subject>isopentenyl adenosine</subject><subject>isopentenyl-9-glucoside</subject><subject>isopentenyladenine</subject><subject>Life Sciences</subject><subject>organogenesis</subject><subject>Original Paper</subject><subject>plant extracts</subject><subject>Plant Genetics and Genomics</subject><subject>plant hormones</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Zeatin</subject><subject>Zeatin riboside</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kEur1TAUhYsoeLz6AxwZEHFU3Xk3Q734ggMO9I5DTh49vbZJTVK1_94eelFw4GgP9rc-FqtpnmJ4hQHk64IxYdACdK1iHW7Xe80Bc0lbDozdbw6AhWxFx-XD5lEptwAgKMOHxvkQvK0oBWTXmr4NcYgFpYj6nH7WMzLRoeJ_LWZEKfcmIud_-DHNk48VDRHVs0e9mXxN83mt_uJ5O3p7jsuEyjxYs2HHx82DYMbin9zdq-bm_buv1x_b4-cPn67fHFvLgNTWCNtx78BRANcZQk4hCCqBWBuEIlh44M5QHsyJA3GBitA5yU-KUUUlBXrVvNy9c07fF1-qnoZi_Tia6NNStAJCWUex2sjn_5C3aclxK6cJ4Yp2BEu-UXinbE6lZB_0nIfJ5FVj0JfZ9T673mbXl9n1umVe3JlNsWYM2UQ7lD9BgkFJgS9usnNle8Xe578N_id_toeCSdr0eRPffCGAKWCugAGlvwHiIpvM</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Menéndez, V</creator><creator>Revilla, M. A</creator><creator>Fal, M. A</creator><creator>Fernández, H</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20090301</creationdate><title>effect of cytokinins on growth and sexual organ development in the gametophyte of Blechnum spicant L</title><author>Menéndez, V ; Revilla, M. A ; Fal, M. A ; Fernández, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-a6c85ed0d300d8a22bff63702ccf69216e05da35fab502df36f8d75b943937303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adenine</topic><topic>Adenosine</topic><topic>Benzyladenine</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Blechnum spicant</topic><topic>culture media</topic><topic>Cytokinins</topic><topic>Ferns</topic><topic>ferns and fern allies</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gametophytes</topic><topic>germ cells</topic><topic>isopentenyl adenosine</topic><topic>isopentenyl-9-glucoside</topic><topic>isopentenyladenine</topic><topic>Life Sciences</topic><topic>organogenesis</topic><topic>Original Paper</topic><topic>plant extracts</topic><topic>Plant Genetics and Genomics</topic><topic>plant hormones</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Zeatin</topic><topic>Zeatin riboside</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Menéndez, V</creatorcontrib><creatorcontrib>Revilla, M. A</creatorcontrib><creatorcontrib>Fal, M. 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A</au><au>Fal, M. A</au><au>Fernández, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>effect of cytokinins on growth and sexual organ development in the gametophyte of Blechnum spicant L</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2009-03-01</date><risdate>2009</risdate><volume>96</volume><issue>3</issue><spage>245</spage><epage>250</epage><pages>245-250</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><coden>PTCEDJ</coden><abstract>In this study, we report the role of exogenous and endogenous cytokinins on growth and sexual organ development in the fern Blechnum spicant L. Spore-derived gametophytes (SG) were cultured in full-strength Murashige and Skoog (1962) liquid medium supplemented with (a) 4.44 μMN⁶-benzyladenine (BAP), (b) a crude extract from mature female gametophytes, and (c) 4.44 μM BAP in combination with the crude extract from mature gametophytes, respectively. Both BAP and the crude extract delayed the gametophyte development, and this effect was increased when they were added together. With respect to sexual organ development, BAP inhibited the sexual organ formation, while the crude extract favored antheridia formation; however, when added together, the percentage of antheridia decreased. The endogenous level of the cytokinins cis-zeatin (cZ), cis-zeatin-riboside (cZR), dihydrozeatin (DHZ), dihydrozeatin riboside (DHZR), isopentenyl adenine (iP), isopentenyl adenosine (iPR), isopentenyl-9-glucoside (iP9G), trans-zeatin (tZ), and trans-zeatin riboside (tZR) were analyzed in female and male gametophytes of B. spicant L. The endogenous levels of cytokinins tZ, cZ, DHZ, cZR, iP, and iPR were higher in female gametophytes than in male gametophytes, with the endogenous iP and iPR content being increased more than 300 and 400 times, respectively.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><doi>10.1007/s11240-008-9481-y</doi><tpages>6</tpages></addata></record> |
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| subjects | Adenine Adenosine Benzyladenine Biological and medical sciences Biomedical and Life Sciences Biotechnology Blechnum spicant culture media Cytokinins Ferns ferns and fern allies Fundamental and applied biological sciences. Psychology Gametophytes germ cells isopentenyl adenosine isopentenyl-9-glucoside isopentenyladenine Life Sciences organogenesis Original Paper plant extracts Plant Genetics and Genomics plant hormones Plant Pathology Plant Physiology Plant Sciences Zeatin Zeatin riboside |
| title | effect of cytokinins on growth and sexual organ development in the gametophyte of Blechnum spicant L |
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