Detection of Brevipalpus-transmitted viruses in their mite vectors by RT-PCR
The diagnosis of plant diseases caused by Brevipalpus-transmitted viruses (BrTVs) has been done through the analyses of symptoms, transmission electron microscopy, and RT-PCR of infected plant tissues. Here, we report the detection of Citrus leprosis virus C, Orchid fleck virus, Clerodendrum chlorot...
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| Veröffentlicht in: | Experimental & applied acarology 2011-05, Vol.54 (1), p.33-39 |
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| creator | Kubo, K. S Novelli, V. M Bastianel, M Locali-Fabris, E. C Antonioli-Luizon, R Machado, M. A Freitas-Astúa, J |
| description | The diagnosis of plant diseases caused by Brevipalpus-transmitted viruses (BrTVs) has been done through the analyses of symptoms, transmission electron microscopy, and RT-PCR of infected plant tissues. Here, we report the detection of Citrus leprosis virus C, Orchid fleck virus, Clerodendrum chlorotic spot virus and Solanum violaefolium ringspot virus in their viruliferous vectors Brevipalpus spp. using specific primer pairs for each of the viruses. The efficiency of virus transmission by Brevipalpus mites is low, so the detection of these pathogens in their vectors could constitute an important tool for studies involving virus-vector relationships, transmission, and monitoring the pathogen prior to the appearance of symptoms in the field. |
| doi_str_mv | 10.1007/s10493-011-9425-9 |
| format | Article |
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S ; Novelli, V. M ; Bastianel, M ; Locali-Fabris, E. C ; Antonioli-Luizon, R ; Machado, M. A ; Freitas-Astúa, J</creator><creatorcontrib>Kubo, K. S ; Novelli, V. M ; Bastianel, M ; Locali-Fabris, E. C ; Antonioli-Luizon, R ; Machado, M. A ; Freitas-Astúa, J</creatorcontrib><description>The diagnosis of plant diseases caused by Brevipalpus-transmitted viruses (BrTVs) has been done through the analyses of symptoms, transmission electron microscopy, and RT-PCR of infected plant tissues. Here, we report the detection of Citrus leprosis virus C, Orchid fleck virus, Clerodendrum chlorotic spot virus and Solanum violaefolium ringspot virus in their viruliferous vectors Brevipalpus spp. using specific primer pairs for each of the viruses. The efficiency of virus transmission by Brevipalpus mites is low, so the detection of these pathogens in their vectors could constitute an important tool for studies involving virus-vector relationships, transmission, and monitoring the pathogen prior to the appearance of symptoms in the field.</description><identifier>ISSN: 0168-8162</identifier><identifier>EISSN: 1572-9702</identifier><identifier>DOI: 10.1007/s10493-011-9425-9</identifier><identifier>PMID: 21279538</identifier><identifier>CODEN: EAACEM</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Animal Ecology ; Animal Genetics and Genomics ; Animal Systematics/Taxonomy/Biogeography ; Animals ; Arachnid Vectors - virology ; Arachnida ; Biological and medical sciences ; Biomedical and Life Sciences ; Brevipalpus ; Citrus leprosis virus ; Citrus leprosis virus C ; Clerodendrum ; Disease transmission ; DNA Primers ; Entomology ; Fundamental and applied biological sciences. Psychology ; Invertebrates ; Life Sciences ; Medically important nuisances and vectors, pests of stored products and materials: population survey and control ; Microbiology ; Mites ; Mites - virology ; Orchid fleck virus ; Pathogens ; Plant diseases ; Plant Diseases - virology ; plant diseases and disorders ; Plant tissues ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Reverse Transcriptase Polymerase Chain Reaction ; Rhabdoviridae - genetics ; Rhabdoviridae - isolation & purification ; Solanum ; transmission electron microscopy ; Vectors. 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S</creatorcontrib><creatorcontrib>Novelli, V. M</creatorcontrib><creatorcontrib>Bastianel, M</creatorcontrib><creatorcontrib>Locali-Fabris, E. C</creatorcontrib><creatorcontrib>Antonioli-Luizon, R</creatorcontrib><creatorcontrib>Machado, M. A</creatorcontrib><creatorcontrib>Freitas-Astúa, J</creatorcontrib><title>Detection of Brevipalpus-transmitted viruses in their mite vectors by RT-PCR</title><title>Experimental & applied acarology</title><addtitle>Exp Appl Acarol</addtitle><addtitle>Exp Appl Acarol</addtitle><description>The diagnosis of plant diseases caused by Brevipalpus-transmitted viruses (BrTVs) has been done through the analyses of symptoms, transmission electron microscopy, and RT-PCR of infected plant tissues. Here, we report the detection of Citrus leprosis virus C, Orchid fleck virus, Clerodendrum chlorotic spot virus and Solanum violaefolium ringspot virus in their viruliferous vectors Brevipalpus spp. using specific primer pairs for each of the viruses. The efficiency of virus transmission by Brevipalpus mites is low, so the detection of these pathogens in their vectors could constitute an important tool for studies involving virus-vector relationships, transmission, and monitoring the pathogen prior to the appearance of symptoms in the field.</description><subject>Animal Ecology</subject><subject>Animal Genetics and Genomics</subject><subject>Animal Systematics/Taxonomy/Biogeography</subject><subject>Animals</subject><subject>Arachnid Vectors - virology</subject><subject>Arachnida</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Brevipalpus</subject><subject>Citrus leprosis virus</subject><subject>Citrus leprosis virus C</subject><subject>Clerodendrum</subject><subject>Disease transmission</subject><subject>DNA Primers</subject><subject>Entomology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Invertebrates</subject><subject>Life Sciences</subject><subject>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</subject><subject>Microbiology</subject><subject>Mites</subject><subject>Mites - virology</subject><subject>Orchid fleck virus</subject><subject>Pathogens</subject><subject>Plant diseases</subject><subject>Plant Diseases - virology</subject><subject>plant diseases and disorders</subject><subject>Plant tissues</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Rhabdoviridae - genetics</subject><subject>Rhabdoviridae - isolation & purification</subject><subject>Solanum</subject><subject>transmission electron microscopy</subject><subject>Vectors. 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| subjects | Animal Ecology Animal Genetics and Genomics Animal Systematics/Taxonomy/Biogeography Animals Arachnid Vectors - virology Arachnida Biological and medical sciences Biomedical and Life Sciences Brevipalpus Citrus leprosis virus Citrus leprosis virus C Clerodendrum Disease transmission DNA Primers Entomology Fundamental and applied biological sciences. Psychology Invertebrates Life Sciences Medically important nuisances and vectors, pests of stored products and materials: population survey and control Microbiology Mites Mites - virology Orchid fleck virus Pathogens Plant diseases Plant Diseases - virology plant diseases and disorders Plant tissues Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Reverse Transcriptase Polymerase Chain Reaction Rhabdoviridae - genetics Rhabdoviridae - isolation & purification Solanum transmission electron microscopy Vectors. Intermediate hosts Virology virus transmission viruses |
| title | Detection of Brevipalpus-transmitted viruses in their mite vectors by RT-PCR |
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