Inhibitory Effect of Bovine Lactoferrin on Human Parainfluenza Virus Type 2 Infection
Lactoferrin (Lf) is a multifunctional protein that has inhibitory activity against microorganisms. In this study, the effects of Lf on the growth of human parainfluenza virus type 2 (hPIV-2) in LLCMK2 cells were investigated. Lf inhibited cell fusion and hemadsorption induced by hPIV-2. However, vir...
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| Veröffentlicht in: | Journal of Health Science 2010, Vol.56(5), pp.613-617 |
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| creator | Yamamoto, Hidetaka Ura, Yukari Tanemura, Miho Koyama, Aoi Takano, Sayaka Uematsu, Jun Kawano, Mitsuo Tsurudome, Masato O'Brien, Myles Komada, Hiroshi |
| description | Lactoferrin (Lf) is a multifunctional protein that has inhibitory activity against microorganisms. In this study, the effects of Lf on the growth of human parainfluenza virus type 2 (hPIV-2) in LLCMK2 cells were investigated. Lf inhibited cell fusion and hemadsorption induced by hPIV-2. However, virus RNA synthesis was only slightly inhibited by Lf. In addition, indirect immunofluorescence study showed that virus protein syntheses were not completely inhibited by Lf. Using a recombinant, green fluorescence protein-expressing hPIV-2 (rghPIV-2), it was found that virus entry into cells were considerably inhibited by Lf, but cell-to-cell spread was not inhibited. The number of viruses produced by the cells were determined, and it was found that Lf reduced the number of released viruses to about 1/300 compared with that of positive control. Lf bound to cell surface within 10 min at early phase of infection, it assembled gradually, and many aggregates were observed at 120 min. These results indicated that Lf considerably inhibited virus adsorption to the surface of the cells by binding to the cell surface and prevented hPIV-2 infection. |
| doi_str_mv | 10.1248/jhs.56.613 |
| format | Article |
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In this study, the effects of Lf on the growth of human parainfluenza virus type 2 (hPIV-2) in LLCMK2 cells were investigated. Lf inhibited cell fusion and hemadsorption induced by hPIV-2. However, virus RNA synthesis was only slightly inhibited by Lf. In addition, indirect immunofluorescence study showed that virus protein syntheses were not completely inhibited by Lf. Using a recombinant, green fluorescence protein-expressing hPIV-2 (rghPIV-2), it was found that virus entry into cells were considerably inhibited by Lf, but cell-to-cell spread was not inhibited. The number of viruses produced by the cells were determined, and it was found that Lf reduced the number of released viruses to about 1/300 compared with that of positive control. Lf bound to cell surface within 10 min at early phase of infection, it assembled gradually, and many aggregates were observed at 120 min. These results indicated that Lf considerably inhibited virus adsorption to the surface of the cells by binding to the cell surface and prevented hPIV-2 infection.</description><identifier>ISSN: 1344-9702</identifier><identifier>EISSN: 1347-5207</identifier><identifier>DOI: 10.1248/jhs.56.613</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>inhibition ; lactoferrin ; Parainfluenza virus ; parainfluenza virus type 2</subject><ispartof>Journal of Health Science, 2010, Vol.56(5), pp.613-617</ispartof><rights>2010 by The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c620t-949c67f65d050c704c0d66e832b14b095eeeb478f62db1e29676a36c794ea04a3</citedby><cites>FETCH-LOGICAL-c620t-949c67f65d050c704c0d66e832b14b095eeeb478f62db1e29676a36c794ea04a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>Yamamoto, Hidetaka</creatorcontrib><creatorcontrib>Ura, Yukari</creatorcontrib><creatorcontrib>Tanemura, Miho</creatorcontrib><creatorcontrib>Koyama, Aoi</creatorcontrib><creatorcontrib>Takano, Sayaka</creatorcontrib><creatorcontrib>Uematsu, Jun</creatorcontrib><creatorcontrib>Kawano, Mitsuo</creatorcontrib><creatorcontrib>Tsurudome, Masato</creatorcontrib><creatorcontrib>O'Brien, Myles</creatorcontrib><creatorcontrib>Komada, Hiroshi</creatorcontrib><creatorcontrib>cGraduate School of Mie Prefectural College of Nursing</creatorcontrib><creatorcontrib>dDepartment of Microbiology</creatorcontrib><creatorcontrib>aDepartment of Microbiology</creatorcontrib><creatorcontrib>Suzuka University of Medical Science</creatorcontrib><creatorcontrib>Graduate School of Clinical Nutrition</creatorcontrib><creatorcontrib>bDepartment of Microbiology</creatorcontrib><creatorcontrib>Mie University Graduate School of Medicine</creatorcontrib><title>Inhibitory Effect of Bovine Lactoferrin on Human Parainfluenza Virus Type 2 Infection</title><title>Journal of Health Science</title><description>Lactoferrin (Lf) is a multifunctional protein that has inhibitory activity against microorganisms. In this study, the effects of Lf on the growth of human parainfluenza virus type 2 (hPIV-2) in LLCMK2 cells were investigated. Lf inhibited cell fusion and hemadsorption induced by hPIV-2. However, virus RNA synthesis was only slightly inhibited by Lf. In addition, indirect immunofluorescence study showed that virus protein syntheses were not completely inhibited by Lf. Using a recombinant, green fluorescence protein-expressing hPIV-2 (rghPIV-2), it was found that virus entry into cells were considerably inhibited by Lf, but cell-to-cell spread was not inhibited. The number of viruses produced by the cells were determined, and it was found that Lf reduced the number of released viruses to about 1/300 compared with that of positive control. Lf bound to cell surface within 10 min at early phase of infection, it assembled gradually, and many aggregates were observed at 120 min. These results indicated that Lf considerably inhibited virus adsorption to the surface of the cells by binding to the cell surface and prevented hPIV-2 infection.</description><subject>inhibition</subject><subject>lactoferrin</subject><subject>Parainfluenza virus</subject><subject>parainfluenza virus type 2</subject><issn>1344-9702</issn><issn>1347-5207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNpdkE-LFDEQxRtRcF29-AkCHgShx0o6fzon0WXdHRjQw67XkM5UnAw9yZh0L4yf3oztKnipBOr3XlW9pnlNYUUZ79_vd2Ul5ErS7klzQTuuWsFAPf39561WwJ43L0rZAzANPb1o7tdxF4YwpXwi196jm0jy5FN6CBHJxropecw5RJIiuZ0PNpKvNtsQ_Thj_GnJt5DnQu5ORySMrOPZIKT4snnm7Vjw1Z_3srn_fH13ddtuvtysrz5uWicZTK3m2knlpdiCAKeAO9hKiX3HBsoH0AIRB656L9l2oMi0VNJ20inN0QK33WXzdvE95vRjxjKZQygOx9FGTHMxGli9mypdyTf_kfs051iXM5TzToCSnFbq3UK5nErJ6M0xh4PNJ0PBnAM2NWAjpKkBV_hmgQ-4Dc6OKY41tH--rqgd2nEyDKoaQEgQBqg0UNXnoihTopeyOn1YnPZlst_x71Cbp-BGfBwqlnJWP3bczmaDsfsFcTOaGw</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Yamamoto, Hidetaka</creator><creator>Ura, Yukari</creator><creator>Tanemura, Miho</creator><creator>Koyama, Aoi</creator><creator>Takano, Sayaka</creator><creator>Uematsu, Jun</creator><creator>Kawano, Mitsuo</creator><creator>Tsurudome, Masato</creator><creator>O'Brien, Myles</creator><creator>Komada, Hiroshi</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan, Nihon Yakugakkai</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>2010</creationdate><title>Inhibitory Effect of Bovine Lactoferrin on Human Parainfluenza Virus Type 2 Infection</title><author>Yamamoto, Hidetaka ; Ura, Yukari ; Tanemura, Miho ; Koyama, Aoi ; Takano, Sayaka ; Uematsu, Jun ; Kawano, Mitsuo ; Tsurudome, Masato ; O'Brien, Myles ; Komada, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c620t-949c67f65d050c704c0d66e832b14b095eeeb478f62db1e29676a36c794ea04a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>inhibition</topic><topic>lactoferrin</topic><topic>Parainfluenza virus</topic><topic>parainfluenza virus type 2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamamoto, Hidetaka</creatorcontrib><creatorcontrib>Ura, Yukari</creatorcontrib><creatorcontrib>Tanemura, Miho</creatorcontrib><creatorcontrib>Koyama, Aoi</creatorcontrib><creatorcontrib>Takano, Sayaka</creatorcontrib><creatorcontrib>Uematsu, Jun</creatorcontrib><creatorcontrib>Kawano, Mitsuo</creatorcontrib><creatorcontrib>Tsurudome, Masato</creatorcontrib><creatorcontrib>O'Brien, Myles</creatorcontrib><creatorcontrib>Komada, Hiroshi</creatorcontrib><creatorcontrib>cGraduate School of Mie Prefectural College of Nursing</creatorcontrib><creatorcontrib>dDepartment of Microbiology</creatorcontrib><creatorcontrib>aDepartment of Microbiology</creatorcontrib><creatorcontrib>Suzuka University of Medical Science</creatorcontrib><creatorcontrib>Graduate School of Clinical Nutrition</creatorcontrib><creatorcontrib>bDepartment of Microbiology</creatorcontrib><creatorcontrib>Mie University Graduate School of Medicine</creatorcontrib><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of Health Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamamoto, Hidetaka</au><au>Ura, Yukari</au><au>Tanemura, Miho</au><au>Koyama, Aoi</au><au>Takano, Sayaka</au><au>Uematsu, Jun</au><au>Kawano, Mitsuo</au><au>Tsurudome, Masato</au><au>O'Brien, Myles</au><au>Komada, Hiroshi</au><aucorp>cGraduate School of Mie Prefectural College of Nursing</aucorp><aucorp>dDepartment of Microbiology</aucorp><aucorp>aDepartment of Microbiology</aucorp><aucorp>Suzuka University of Medical Science</aucorp><aucorp>Graduate School of Clinical Nutrition</aucorp><aucorp>bDepartment of Microbiology</aucorp><aucorp>Mie University Graduate School of Medicine</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory Effect of Bovine Lactoferrin on Human Parainfluenza Virus Type 2 Infection</atitle><jtitle>Journal of Health Science</jtitle><date>2010</date><risdate>2010</risdate><volume>56</volume><issue>5</issue><spage>613</spage><epage>617</epage><pages>613-617</pages><issn>1344-9702</issn><eissn>1347-5207</eissn><abstract>Lactoferrin (Lf) is a multifunctional protein that has inhibitory activity against microorganisms. In this study, the effects of Lf on the growth of human parainfluenza virus type 2 (hPIV-2) in LLCMK2 cells were investigated. Lf inhibited cell fusion and hemadsorption induced by hPIV-2. However, virus RNA synthesis was only slightly inhibited by Lf. In addition, indirect immunofluorescence study showed that virus protein syntheses were not completely inhibited by Lf. Using a recombinant, green fluorescence protein-expressing hPIV-2 (rghPIV-2), it was found that virus entry into cells were considerably inhibited by Lf, but cell-to-cell spread was not inhibited. The number of viruses produced by the cells were determined, and it was found that Lf reduced the number of released viruses to about 1/300 compared with that of positive control. Lf bound to cell surface within 10 min at early phase of infection, it assembled gradually, and many aggregates were observed at 120 min. These results indicated that Lf considerably inhibited virus adsorption to the surface of the cells by binding to the cell surface and prevented hPIV-2 infection.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><doi>10.1248/jhs.56.613</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | inhibition lactoferrin Parainfluenza virus parainfluenza virus type 2 |
| title | Inhibitory Effect of Bovine Lactoferrin on Human Parainfluenza Virus Type 2 Infection |
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