Testing and Isolation of High-Purity Restriction Endonucleases

A new method of testing restriction nucleases is proposed. This method is based on high-temperature treatment of crude cell extracts. Disrupted cells were heated at 50-60°C, centrifuged, and assayed for restrictases. This method provides the opportunity for screening new enzymes in microbial strains...

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Veröffentlicht in:	Applied biochemistry and microbiology 2002-01, Vol.38 (1), p.15-19
Hauptverfasser:	Puchkova, L I, Ushakova, T A, Mikhailova, V K, Serov, G D, Krivopalova, G N, Repin, V E
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Enzymatic activity High temperature Microorganisms
Online-Zugang:	Volltext
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