Rhizobium pusense sp. nov., isolated from the rhizosphere of chickpea (Cicer arietinum L.)

A novel bacterial strain, designated NRCPB10T, was isolated from rhizosphere soil of chickpea (Cicer arietinum L.) in Pusa, New Delhi, India. The 16S rRNA gene sequence of strain NRCPB10T showed highest similarity (98.9 %) to that of Rhizobium radiobacter NCPPB 2437T, followed by Rhizobium larrymoor...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:International journal of systematic and evolutionary microbiology 2011-11, Vol.61 (Pt 11), p.2632-2639
Hauptverfasser: Panday, Digvijay, Schumann, Peter, Das, Subrata K
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 2639
container_issue Pt 11
container_start_page 2632
container_title International journal of systematic and evolutionary microbiology
container_volume 61
creator Panday, Digvijay
Schumann, Peter
Das, Subrata K
description A novel bacterial strain, designated NRCPB10T, was isolated from rhizosphere soil of chickpea (Cicer arietinum L.) in Pusa, New Delhi, India. The 16S rRNA gene sequence of strain NRCPB10T showed highest similarity (98.9 %) to that of Rhizobium radiobacter NCPPB 2437T, followed by Rhizobium larrymoorei AF3-10T (97.7 %) and Rhizobium rubi IFO 13261T (97.4 %). Phylogenetic analysis of strain NRCPB10T based on the housekeeping genes recA and atpD confirmed its position as distinct from recognized Rhizobium species. Levels of DNA–DNA relatedness between strain NRCPB10T and R. radiobacter ICMP 5785T, R. larrymoorei LMG 21410T and R. rubi ICMP 6428T were 51.0, 32.6 and 27.3 %, respectively. Cellular fatty acids of strain NRCPB10T were C18 : 1ω7c (58.9 %), C16 : 0 (15.5 %), C19 : 0 cyclo ω8c (11.5 %), iso-C16 : 1 (5.8 %), C16 : 0 3-OH (4.5 %), C16 : 1ω7c (2.1 %) and C18 : 0 (1.3 %). The G+C content of the genomic DNA of strain NRCPB10T was 59.0 mol%. Strain NRCPB10T did not nodulate chickpea plants or induce tumours in tobacco plants. Phenotypic and physiological properties along with SDS-PAGE of whole-cell soluble proteins differentiated strain NRCPB10T from its closest phylogenetic neighbours. On the basis of data from the present polyphasic taxonomic study, strain NRCPB10T is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium pusense sp. nov. is proposed. The type strain is NRCPB10T ( = LMG 25623T = JCM 16209T = NCIMB 14639T).
doi_str_mv 10.1099/ijs.0.028407-0
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_902330703</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>902330703</sourcerecordid><originalsourceid>FETCH-LOGICAL-c454t-69037fdf40e497f7ed7c2a168ace1b678c51f8a57964c3e35042ee3a3595d4e63</originalsourceid><addsrcrecordid>eNpN0E1v1DAQBmCrompL6bVH8AVBJRLG8VdyRKuWVloJCeiFi-V1xqxLEgc7qQS_nqx2KZw8sp55R3oJuWRQMmia9-Ehl1BCVQvQBRyRMyaUKiRw8ey_-ZQ8z_kBYPkAOCGnFWOiVlqdkW-ft-F33IS5p-OccchI81jSIT6W72jIsbMTttSn2NNpizTtdB63mJBGT902uB8jWvp2FRwmalPAKQxL2Lq8ekGOve0yXhzec3J_c_11dVusP328W31YF05IMRWqAa596wWgaLTX2GpXWaZq65BtlK6dZL62UjdKOI5cgqgQueWyka1Axc_Jm33umOLPGfNk-pAddp0dMM7ZNFBxDhr4Isu9dCnmnNCbMYXepl-GgdnVaZY6DZh9nQaWhZeH6HnTY_vE__a3gNcHYLOznU92cCH_c0ILxvXu8qu98zYa-z0t5v5LBUwCgFI1k_wP-ymFzQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>902330703</pqid></control><display><type>article</type><title>Rhizobium pusense sp. nov., isolated from the rhizosphere of chickpea (Cicer arietinum L.)</title><source>MEDLINE</source><source>Microbiology Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Panday, Digvijay ; Schumann, Peter ; Das, Subrata K</creator><creatorcontrib>Panday, Digvijay ; Schumann, Peter ; Das, Subrata K</creatorcontrib><description>A novel bacterial strain, designated NRCPB10T, was isolated from rhizosphere soil of chickpea (Cicer arietinum L.) in Pusa, New Delhi, India. The 16S rRNA gene sequence of strain NRCPB10T showed highest similarity (98.9 %) to that of Rhizobium radiobacter NCPPB 2437T, followed by Rhizobium larrymoorei AF3-10T (97.7 %) and Rhizobium rubi IFO 13261T (97.4 %). Phylogenetic analysis of strain NRCPB10T based on the housekeeping genes recA and atpD confirmed its position as distinct from recognized Rhizobium species. Levels of DNA–DNA relatedness between strain NRCPB10T and R. radiobacter ICMP 5785T, R. larrymoorei LMG 21410T and R. rubi ICMP 6428T were 51.0, 32.6 and 27.3 %, respectively. Cellular fatty acids of strain NRCPB10T were C18 : 1ω7c (58.9 %), C16 : 0 (15.5 %), C19 : 0 cyclo ω8c (11.5 %), iso-C16 : 1 (5.8 %), C16 : 0 3-OH (4.5 %), C16 : 1ω7c (2.1 %) and C18 : 0 (1.3 %). The G+C content of the genomic DNA of strain NRCPB10T was 59.0 mol%. Strain NRCPB10T did not nodulate chickpea plants or induce tumours in tobacco plants. Phenotypic and physiological properties along with SDS-PAGE of whole-cell soluble proteins differentiated strain NRCPB10T from its closest phylogenetic neighbours. On the basis of data from the present polyphasic taxonomic study, strain NRCPB10T is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium pusense sp. nov. is proposed. The type strain is NRCPB10T ( = LMG 25623T = JCM 16209T = NCIMB 14639T).</description><identifier>ISSN: 1466-5034</identifier><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijs.0.028407-0</identifier><identifier>PMID: 21148676</identifier><language>eng</language><publisher>Reading: International Union of Microbiological Societies</publisher><subject>Agrobacterium radiobacter ; Bacteriology ; Base Composition ; Biological and medical sciences ; chickpeas ; Cicer - microbiology ; Cicer arietinum ; DNA ; DNA, Bacterial - genetics ; essential genes ; fatty acids ; Fatty Acids - metabolism ; Fundamental and applied biological sciences. Psychology ; India ; Microbiology ; Molecular Sequence Data ; neoplasms ; new species ; nucleotide sequences ; Phylogeny ; polyacrylamide gel electrophoresis ; proteins ; Rhizobium - classification ; Rhizobium - genetics ; Rhizobium - isolation &amp; purification ; Rhizobium - metabolism ; Rhizobium rubi ; Rhizosphere ; ribosomal RNA ; RNA, Ribosomal, 16S - genetics ; soil ; Soil Microbiology ; Systematics ; tobacco</subject><ispartof>International journal of systematic and evolutionary microbiology, 2011-11, Vol.61 (Pt 11), p.2632-2639</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-69037fdf40e497f7ed7c2a168ace1b678c51f8a57964c3e35042ee3a3595d4e63</citedby><cites>FETCH-LOGICAL-c454t-69037fdf40e497f7ed7c2a168ace1b678c51f8a57964c3e35042ee3a3595d4e63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=24741373$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21148676$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Panday, Digvijay</creatorcontrib><creatorcontrib>Schumann, Peter</creatorcontrib><creatorcontrib>Das, Subrata K</creatorcontrib><title>Rhizobium pusense sp. nov., isolated from the rhizosphere of chickpea (Cicer arietinum L.)</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>A novel bacterial strain, designated NRCPB10T, was isolated from rhizosphere soil of chickpea (Cicer arietinum L.) in Pusa, New Delhi, India. The 16S rRNA gene sequence of strain NRCPB10T showed highest similarity (98.9 %) to that of Rhizobium radiobacter NCPPB 2437T, followed by Rhizobium larrymoorei AF3-10T (97.7 %) and Rhizobium rubi IFO 13261T (97.4 %). Phylogenetic analysis of strain NRCPB10T based on the housekeeping genes recA and atpD confirmed its position as distinct from recognized Rhizobium species. Levels of DNA–DNA relatedness between strain NRCPB10T and R. radiobacter ICMP 5785T, R. larrymoorei LMG 21410T and R. rubi ICMP 6428T were 51.0, 32.6 and 27.3 %, respectively. Cellular fatty acids of strain NRCPB10T were C18 : 1ω7c (58.9 %), C16 : 0 (15.5 %), C19 : 0 cyclo ω8c (11.5 %), iso-C16 : 1 (5.8 %), C16 : 0 3-OH (4.5 %), C16 : 1ω7c (2.1 %) and C18 : 0 (1.3 %). The G+C content of the genomic DNA of strain NRCPB10T was 59.0 mol%. Strain NRCPB10T did not nodulate chickpea plants or induce tumours in tobacco plants. Phenotypic and physiological properties along with SDS-PAGE of whole-cell soluble proteins differentiated strain NRCPB10T from its closest phylogenetic neighbours. On the basis of data from the present polyphasic taxonomic study, strain NRCPB10T is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium pusense sp. nov. is proposed. The type strain is NRCPB10T ( = LMG 25623T = JCM 16209T = NCIMB 14639T).</description><subject>Agrobacterium radiobacter</subject><subject>Bacteriology</subject><subject>Base Composition</subject><subject>Biological and medical sciences</subject><subject>chickpeas</subject><subject>Cicer - microbiology</subject><subject>Cicer arietinum</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>essential genes</subject><subject>fatty acids</subject><subject>Fatty Acids - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>India</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>neoplasms</subject><subject>new species</subject><subject>nucleotide sequences</subject><subject>Phylogeny</subject><subject>polyacrylamide gel electrophoresis</subject><subject>proteins</subject><subject>Rhizobium - classification</subject><subject>Rhizobium - genetics</subject><subject>Rhizobium - isolation &amp; purification</subject><subject>Rhizobium - metabolism</subject><subject>Rhizobium rubi</subject><subject>Rhizosphere</subject><subject>ribosomal RNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>soil</subject><subject>Soil Microbiology</subject><subject>Systematics</subject><subject>tobacco</subject><issn>1466-5034</issn><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpN0E1v1DAQBmCrompL6bVH8AVBJRLG8VdyRKuWVloJCeiFi-V1xqxLEgc7qQS_nqx2KZw8sp55R3oJuWRQMmia9-Ehl1BCVQvQBRyRMyaUKiRw8ey_-ZQ8z_kBYPkAOCGnFWOiVlqdkW-ft-F33IS5p-OccchI81jSIT6W72jIsbMTttSn2NNpizTtdB63mJBGT902uB8jWvp2FRwmalPAKQxL2Lq8ekGOve0yXhzec3J_c_11dVusP328W31YF05IMRWqAa596wWgaLTX2GpXWaZq65BtlK6dZL62UjdKOI5cgqgQueWyka1Axc_Jm33umOLPGfNk-pAddp0dMM7ZNFBxDhr4Isu9dCnmnNCbMYXepl-GgdnVaZY6DZh9nQaWhZeH6HnTY_vE__a3gNcHYLOznU92cCH_c0ILxvXu8qu98zYa-z0t5v5LBUwCgFI1k_wP-ymFzQ</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>Panday, Digvijay</creator><creator>Schumann, Peter</creator><creator>Das, Subrata K</creator><general>International Union of Microbiological Societies</general><general>Society for General Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20111101</creationdate><title>Rhizobium pusense sp. nov., isolated from the rhizosphere of chickpea (Cicer arietinum L.)</title><author>Panday, Digvijay ; Schumann, Peter ; Das, Subrata K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-69037fdf40e497f7ed7c2a168ace1b678c51f8a57964c3e35042ee3a3595d4e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Agrobacterium radiobacter</topic><topic>Bacteriology</topic><topic>Base Composition</topic><topic>Biological and medical sciences</topic><topic>chickpeas</topic><topic>Cicer - microbiology</topic><topic>Cicer arietinum</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>essential genes</topic><topic>fatty acids</topic><topic>Fatty Acids - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>India</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>neoplasms</topic><topic>new species</topic><topic>nucleotide sequences</topic><topic>Phylogeny</topic><topic>polyacrylamide gel electrophoresis</topic><topic>proteins</topic><topic>Rhizobium - classification</topic><topic>Rhizobium - genetics</topic><topic>Rhizobium - isolation &amp; purification</topic><topic>Rhizobium - metabolism</topic><topic>Rhizobium rubi</topic><topic>Rhizosphere</topic><topic>ribosomal RNA</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>soil</topic><topic>Soil Microbiology</topic><topic>Systematics</topic><topic>tobacco</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Panday, Digvijay</creatorcontrib><creatorcontrib>Schumann, Peter</creatorcontrib><creatorcontrib>Das, Subrata K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Panday, Digvijay</au><au>Schumann, Peter</au><au>Das, Subrata K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rhizobium pusense sp. nov., isolated from the rhizosphere of chickpea (Cicer arietinum L.)</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2011-11-01</date><risdate>2011</risdate><volume>61</volume><issue>Pt 11</issue><spage>2632</spage><epage>2639</epage><pages>2632-2639</pages><issn>1466-5034</issn><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>A novel bacterial strain, designated NRCPB10T, was isolated from rhizosphere soil of chickpea (Cicer arietinum L.) in Pusa, New Delhi, India. The 16S rRNA gene sequence of strain NRCPB10T showed highest similarity (98.9 %) to that of Rhizobium radiobacter NCPPB 2437T, followed by Rhizobium larrymoorei AF3-10T (97.7 %) and Rhizobium rubi IFO 13261T (97.4 %). Phylogenetic analysis of strain NRCPB10T based on the housekeeping genes recA and atpD confirmed its position as distinct from recognized Rhizobium species. Levels of DNA–DNA relatedness between strain NRCPB10T and R. radiobacter ICMP 5785T, R. larrymoorei LMG 21410T and R. rubi ICMP 6428T were 51.0, 32.6 and 27.3 %, respectively. Cellular fatty acids of strain NRCPB10T were C18 : 1ω7c (58.9 %), C16 : 0 (15.5 %), C19 : 0 cyclo ω8c (11.5 %), iso-C16 : 1 (5.8 %), C16 : 0 3-OH (4.5 %), C16 : 1ω7c (2.1 %) and C18 : 0 (1.3 %). The G+C content of the genomic DNA of strain NRCPB10T was 59.0 mol%. Strain NRCPB10T did not nodulate chickpea plants or induce tumours in tobacco plants. Phenotypic and physiological properties along with SDS-PAGE of whole-cell soluble proteins differentiated strain NRCPB10T from its closest phylogenetic neighbours. On the basis of data from the present polyphasic taxonomic study, strain NRCPB10T is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium pusense sp. nov. is proposed. The type strain is NRCPB10T ( = LMG 25623T = JCM 16209T = NCIMB 14639T).</abstract><cop>Reading</cop><pub>International Union of Microbiological Societies</pub><pmid>21148676</pmid><doi>10.1099/ijs.0.028407-0</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1466-5034
ispartof International journal of systematic and evolutionary microbiology, 2011-11, Vol.61 (Pt 11), p.2632-2639
issn 1466-5034
1466-5026
1466-5034
language eng
recordid cdi_proquest_miscellaneous_902330703
source MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Agrobacterium radiobacter
Bacteriology
Base Composition
Biological and medical sciences
chickpeas
Cicer - microbiology
Cicer arietinum
DNA
DNA, Bacterial - genetics
essential genes
fatty acids
Fatty Acids - metabolism
Fundamental and applied biological sciences. Psychology
India
Microbiology
Molecular Sequence Data
neoplasms
new species
nucleotide sequences
Phylogeny
polyacrylamide gel electrophoresis
proteins
Rhizobium - classification
Rhizobium - genetics
Rhizobium - isolation & purification
Rhizobium - metabolism
Rhizobium rubi
Rhizosphere
ribosomal RNA
RNA, Ribosomal, 16S - genetics
soil
Soil Microbiology
Systematics
tobacco
title Rhizobium pusense sp. nov., isolated from the rhizosphere of chickpea (Cicer arietinum L.)
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T13%3A12%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rhizobium%20pusense%20sp.%20nov.,%20isolated%20from%20the%20rhizosphere%20of%20chickpea%20(Cicer%20arietinum%20L.)&rft.jtitle=International%20journal%20of%20systematic%20and%20evolutionary%20microbiology&rft.au=Panday,%20Digvijay&rft.date=2011-11-01&rft.volume=61&rft.issue=Pt%2011&rft.spage=2632&rft.epage=2639&rft.pages=2632-2639&rft.issn=1466-5034&rft.eissn=1466-5034&rft_id=info:doi/10.1099/ijs.0.028407-0&rft_dat=%3Cproquest_cross%3E902330703%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=902330703&rft_id=info:pmid/21148676&rfr_iscdi=true