A new method for the radiochemical purity measurement of 111In-pentetreotide
OBJECTIVES AND METHODSThe recommended method for the measurement of radiochemical purity (RCP) of In-labelled pentetreotide is thin-layer chromatography with a silica gel as the stationary phase and a 0.1 N sodium citrate solution (pH 5) as the mobile phase. According to the supplier’s instructions,...
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| Veröffentlicht in: | Nuclear medicine communications 2011-12, Vol.32 (12), p.1241-1244 |
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| creator | Salgado-Garcia, Carlos Montoza-Aguado, Manuel Luna-Alcaide, Ana B Segovia-Gonzalez, Maria M de Mora, Elena Sanchez Lopez-Martin, Juana Ramos-Font, Carlos Jimenez-Heffernan, Amelia |
| description | OBJECTIVES AND METHODSThe recommended method for the measurement of radiochemical purity (RCP) of In-labelled pentetreotide is thin-layer chromatography with a silica gel as the stationary phase and a 0.1 N sodium citrate solution (pH 5) as the mobile phase. According to the supplier’s instructions, the mobile phase must be prepared before the test is carried out, and the recommended stationary phase is off-market. We propose a new method for RCP measurement in which the mobile phase is acid citrate dextrose, solution A, which does not need to be prepared beforehand, and thin-layer chromatography is performed with a silica gel-impregnated glass fibre sheet as the stationary phase. We used both methods to measure the percentages of radiopharmaceutical and impurities.
RESULTSThe range of RCP values obtained was 98.0–99.9% (mean=99.3%) by the standard method and 98.1–99.9% (mean=99.2%) by the new method. We observed no differences between the RCP values of both methods (P=0.070).
CONCLUSIONThe proposed method is suitable for RCP testing because it yields results that are in good agreement with those of the standard method and because it is easier to perform as the mobile-phase solution need not be prepared in advance. |
| doi_str_mv | 10.1097/MNM.0b013e32834a7003 |
| format | Article |
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RESULTSThe range of RCP values obtained was 98.0–99.9% (mean=99.3%) by the standard method and 98.1–99.9% (mean=99.2%) by the new method. We observed no differences between the RCP values of both methods (P=0.070).
CONCLUSIONThe proposed method is suitable for RCP testing because it yields results that are in good agreement with those of the standard method and because it is easier to perform as the mobile-phase solution need not be prepared in advance.</description><identifier>ISSN: 0143-3636</identifier><identifier>EISSN: 1473-5628</identifier><identifier>DOI: 10.1097/MNM.0b013e32834a7003</identifier><identifier>PMID: 21946620</identifier><language>eng</language><publisher>England: Lippincott Williams & Wilkins, Inc</publisher><subject>Chromatography, Thin Layer - methods ; Citric Acid ; Glass ; Glucose - analogs & derivatives ; Humans ; Indium Radioisotopes ; Radiochemistry - methods ; Radiopharmaceuticals - analysis ; Radiopharmaceuticals - standards ; Reproducibility of Results ; Silica Gel ; Somatostatin - analogs & derivatives ; Somatostatin - analysis ; Somatostatin - standards</subject><ispartof>Nuclear medicine communications, 2011-12, Vol.32 (12), p.1241-1244</ispartof><rights>2011 Lippincott Williams & Wilkins, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2003-bba7aae3eae437ade3570a9165b098219c9e95826508f9992496ca6af1da65b23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21946620$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salgado-Garcia, Carlos</creatorcontrib><creatorcontrib>Montoza-Aguado, Manuel</creatorcontrib><creatorcontrib>Luna-Alcaide, Ana B</creatorcontrib><creatorcontrib>Segovia-Gonzalez, Maria M</creatorcontrib><creatorcontrib>de Mora, Elena Sanchez</creatorcontrib><creatorcontrib>Lopez-Martin, Juana</creatorcontrib><creatorcontrib>Ramos-Font, Carlos</creatorcontrib><creatorcontrib>Jimenez-Heffernan, Amelia</creatorcontrib><title>A new method for the radiochemical purity measurement of 111In-pentetreotide</title><title>Nuclear medicine communications</title><addtitle>Nucl Med Commun</addtitle><description>OBJECTIVES AND METHODSThe recommended method for the measurement of radiochemical purity (RCP) of In-labelled pentetreotide is thin-layer chromatography with a silica gel as the stationary phase and a 0.1 N sodium citrate solution (pH 5) as the mobile phase. According to the supplier’s instructions, the mobile phase must be prepared before the test is carried out, and the recommended stationary phase is off-market. We propose a new method for RCP measurement in which the mobile phase is acid citrate dextrose, solution A, which does not need to be prepared beforehand, and thin-layer chromatography is performed with a silica gel-impregnated glass fibre sheet as the stationary phase. We used both methods to measure the percentages of radiopharmaceutical and impurities.
RESULTSThe range of RCP values obtained was 98.0–99.9% (mean=99.3%) by the standard method and 98.1–99.9% (mean=99.2%) by the new method. We observed no differences between the RCP values of both methods (P=0.070).
CONCLUSIONThe proposed method is suitable for RCP testing because it yields results that are in good agreement with those of the standard method and because it is easier to perform as the mobile-phase solution need not be prepared in advance.</description><subject>Chromatography, Thin Layer - methods</subject><subject>Citric Acid</subject><subject>Glass</subject><subject>Glucose - analogs & derivatives</subject><subject>Humans</subject><subject>Indium Radioisotopes</subject><subject>Radiochemistry - methods</subject><subject>Radiopharmaceuticals - analysis</subject><subject>Radiopharmaceuticals - standards</subject><subject>Reproducibility of Results</subject><subject>Silica Gel</subject><subject>Somatostatin - analogs & derivatives</subject><subject>Somatostatin - analysis</subject><subject>Somatostatin - standards</subject><issn>0143-3636</issn><issn>1473-5628</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQhi0EglL4BwhlYwqc7cSJx6rio1ILC8zRJbkogaQutqOq_x6jFgYGptNJz_ve6WHsisMtB53drZ5Xt1AClyRFLhPMAOQRm_Akk3GqRH7MJsATGUsl1Rk7d-4dAHKpslN2JrhOlBIwYctZtKZtNJBvTR01xka-pchi3ZmqpaGrsI82o-38LjDoRksDrX1kmohzvljHm7CRt2R8V9MFO2mwd3R5mFP29nD_On-Kly-Pi_lsGVciPBmXJWaIJAkpkRnWJNMMUHOVlqDz8FulSae5UCnkjdZaJFpVqLDhNQZGyCm72fdurPkcyfli6FxFfY9rMqMrNAjI00zyQCZ7srLGOUtNsbHdgHZXcCi-NRZBY_FXY4hdHw6M5UD1b-jHWwDyPbA1vSfrPvpxS7ZoCXvf_t_9Benhf9Q</recordid><startdate>201112</startdate><enddate>201112</enddate><creator>Salgado-Garcia, Carlos</creator><creator>Montoza-Aguado, Manuel</creator><creator>Luna-Alcaide, Ana B</creator><creator>Segovia-Gonzalez, Maria M</creator><creator>de Mora, Elena Sanchez</creator><creator>Lopez-Martin, Juana</creator><creator>Ramos-Font, Carlos</creator><creator>Jimenez-Heffernan, Amelia</creator><general>Lippincott Williams & Wilkins, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201112</creationdate><title>A new method for the radiochemical purity measurement of 111In-pentetreotide</title><author>Salgado-Garcia, Carlos ; Montoza-Aguado, Manuel ; Luna-Alcaide, Ana B ; Segovia-Gonzalez, Maria M ; de Mora, Elena Sanchez ; Lopez-Martin, Juana ; Ramos-Font, Carlos ; Jimenez-Heffernan, Amelia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2003-bba7aae3eae437ade3570a9165b098219c9e95826508f9992496ca6af1da65b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Chromatography, Thin Layer - methods</topic><topic>Citric Acid</topic><topic>Glass</topic><topic>Glucose - analogs & derivatives</topic><topic>Humans</topic><topic>Indium Radioisotopes</topic><topic>Radiochemistry - methods</topic><topic>Radiopharmaceuticals - analysis</topic><topic>Radiopharmaceuticals - standards</topic><topic>Reproducibility of Results</topic><topic>Silica Gel</topic><topic>Somatostatin - analogs & derivatives</topic><topic>Somatostatin - analysis</topic><topic>Somatostatin - standards</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salgado-Garcia, Carlos</creatorcontrib><creatorcontrib>Montoza-Aguado, Manuel</creatorcontrib><creatorcontrib>Luna-Alcaide, Ana B</creatorcontrib><creatorcontrib>Segovia-Gonzalez, Maria M</creatorcontrib><creatorcontrib>de Mora, Elena Sanchez</creatorcontrib><creatorcontrib>Lopez-Martin, Juana</creatorcontrib><creatorcontrib>Ramos-Font, Carlos</creatorcontrib><creatorcontrib>Jimenez-Heffernan, Amelia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nuclear medicine communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salgado-Garcia, Carlos</au><au>Montoza-Aguado, Manuel</au><au>Luna-Alcaide, Ana B</au><au>Segovia-Gonzalez, Maria M</au><au>de Mora, Elena Sanchez</au><au>Lopez-Martin, Juana</au><au>Ramos-Font, Carlos</au><au>Jimenez-Heffernan, Amelia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new method for the radiochemical purity measurement of 111In-pentetreotide</atitle><jtitle>Nuclear medicine communications</jtitle><addtitle>Nucl Med Commun</addtitle><date>2011-12</date><risdate>2011</risdate><volume>32</volume><issue>12</issue><spage>1241</spage><epage>1244</epage><pages>1241-1244</pages><issn>0143-3636</issn><eissn>1473-5628</eissn><abstract>OBJECTIVES AND METHODSThe recommended method for the measurement of radiochemical purity (RCP) of In-labelled pentetreotide is thin-layer chromatography with a silica gel as the stationary phase and a 0.1 N sodium citrate solution (pH 5) as the mobile phase. According to the supplier’s instructions, the mobile phase must be prepared before the test is carried out, and the recommended stationary phase is off-market. We propose a new method for RCP measurement in which the mobile phase is acid citrate dextrose, solution A, which does not need to be prepared beforehand, and thin-layer chromatography is performed with a silica gel-impregnated glass fibre sheet as the stationary phase. We used both methods to measure the percentages of radiopharmaceutical and impurities.
RESULTSThe range of RCP values obtained was 98.0–99.9% (mean=99.3%) by the standard method and 98.1–99.9% (mean=99.2%) by the new method. We observed no differences between the RCP values of both methods (P=0.070).
CONCLUSIONThe proposed method is suitable for RCP testing because it yields results that are in good agreement with those of the standard method and because it is easier to perform as the mobile-phase solution need not be prepared in advance.</abstract><cop>England</cop><pub>Lippincott Williams & Wilkins, Inc</pub><pmid>21946620</pmid><doi>10.1097/MNM.0b013e32834a7003</doi><tpages>4</tpages></addata></record> |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Chromatography, Thin Layer - methods Citric Acid Glass Glucose - analogs & derivatives Humans Indium Radioisotopes Radiochemistry - methods Radiopharmaceuticals - analysis Radiopharmaceuticals - standards Reproducibility of Results Silica Gel Somatostatin - analogs & derivatives Somatostatin - analysis Somatostatin - standards |
| title | A new method for the radiochemical purity measurement of 111In-pentetreotide |
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