Photobiological hydrogen production from Synechocystis sp. PCC 6803 encapsulated in silica sol–gel
Hydrogen production was measured from viable wild-type and M55 mutant cells of Synechocystis sp. PCC 6803 encapsulated in silica sol–gel derived from tetraethoxysilane, tetramethoxysilane, and a mixture of tetraethoxysilane with methyltriethoxysilane. Glycerol and polyethylene glycol were used as ad...
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| Veröffentlicht in: | International journal of hydrogen energy 2009, Vol.34 (1), p.204-215 |
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| creator | Dickson, David J. Page, Catherine J. Ely, Roger L. |
| description | Hydrogen production was measured from viable wild-type and M55 mutant cells of
Synechocystis sp. PCC 6803 encapsulated in silica sol–gel derived from tetraethoxysilane, tetramethoxysilane, and a mixture of tetraethoxysilane with methyltriethoxysilane. Glycerol and polyethylene glycol were used as additives in concentrations up to 14
wt.% to increase porosity and pore connectivity and to reduce stress on the encapsulated cells. Mixtures were evaluated for hydrogen production in a high-throughput screening assay, and samples were imaged with environmental scanning electron microscopy. Compositions showing improved H
2 production were selected for further study via quantitative analysis of H
2 production in GC vial assays. H
2 production activity was monitored for up to 5 days and H
2 production from encapsulated cells was observed at levels comparable to or exceeding cells suspended in liquid media. |
| doi_str_mv | 10.1016/j.ijhydene.2008.10.021 |
| format | Article |
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Synechocystis sp. PCC 6803 encapsulated in silica sol–gel derived from tetraethoxysilane, tetramethoxysilane, and a mixture of tetraethoxysilane with methyltriethoxysilane. Glycerol and polyethylene glycol were used as additives in concentrations up to 14
wt.% to increase porosity and pore connectivity and to reduce stress on the encapsulated cells. Mixtures were evaluated for hydrogen production in a high-throughput screening assay, and samples were imaged with environmental scanning electron microscopy. Compositions showing improved H
2 production were selected for further study via quantitative analysis of H
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wt.% to increase porosity and pore connectivity and to reduce stress on the encapsulated cells. Mixtures were evaluated for hydrogen production in a high-throughput screening assay, and samples were imaged with environmental scanning electron microscopy. Compositions showing improved H
2 production were selected for further study via quantitative analysis of H
2 production in GC vial assays. H
2 production activity was monitored for up to 5 days and H
2 production from encapsulated cells was observed at levels comparable to or exceeding cells suspended in liquid media.</description><subject>Alternative fuels. Production and utilization</subject><subject>Applied sciences</subject><subject>Biophotolysis</subject><subject>Biosolar</subject><subject>Cyanobacteria</subject><subject>Energy</subject><subject>Exact sciences and technology</subject><subject>Fuels</subject><subject>Glycerol</subject><subject>Hydrogen</subject><subject>Hydrogenase</subject><subject>Polyethylene glycol</subject><subject>Synechocystis</subject><subject>Tetraethoxysilane</subject><subject>Tetramethoxysilane</subject><issn>0360-3199</issn><issn>1879-3487</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkM1qGzEUhUVpoW7aVyjalKxmop8ZSbNrME0TCCTQdi0U6Y4tI48caRzwru_QN-yT9Bon3WYlcTj3nHs_Qj5z1nLG1cWmjZv1IcAErWDMoNgywd-QBTd6aGRn9FuyYFKxRvJheE8-1LphjGvWDQsS7td5zg8xp7yK3iWKSSWvYKK7ksPezzFPdCx5S38cJvDr7A91jpXWXUvvl0uqDJMUJu92dZ_cDIHGidaYMIvWnP7-_rOC9JG8G12q8On5PSO_rr79XF43t3ffb5aXt42Xupsb2eNSUgx-7IRyDByEIAzzTBkdJP4F6NDzzkghzIPpe61RwFOUkqMTRp6R81Mu7v64hzrbbaweUnIT5H21A1qxoVPoVCenL7nWAqPdlbh15WA5s0eqdmNfqNoj1aOOVHHwy3OFq4hrLG7ysf6fFpz1XGiJvq8nH-C9TxGKrT4iJwixgJ9tyPG1qn_kFpGM</recordid><startdate>2009</startdate><enddate>2009</enddate><creator>Dickson, David J.</creator><creator>Page, Catherine J.</creator><creator>Ely, Roger L.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope></search><sort><creationdate>2009</creationdate><title>Photobiological hydrogen production from Synechocystis sp. PCC 6803 encapsulated in silica sol–gel</title><author>Dickson, David J. ; Page, Catherine J. ; Ely, Roger L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-35170329cf426a0eaedd280c0687d3dd22e7d51483228b85577e7d001663fa283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Alternative fuels. Production and utilization</topic><topic>Applied sciences</topic><topic>Biophotolysis</topic><topic>Biosolar</topic><topic>Cyanobacteria</topic><topic>Energy</topic><topic>Exact sciences and technology</topic><topic>Fuels</topic><topic>Glycerol</topic><topic>Hydrogen</topic><topic>Hydrogenase</topic><topic>Polyethylene glycol</topic><topic>Synechocystis</topic><topic>Tetraethoxysilane</topic><topic>Tetramethoxysilane</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dickson, David J.</creatorcontrib><creatorcontrib>Page, Catherine J.</creatorcontrib><creatorcontrib>Ely, Roger L.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>International journal of hydrogen energy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dickson, David J.</au><au>Page, Catherine J.</au><au>Ely, Roger L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photobiological hydrogen production from Synechocystis sp. PCC 6803 encapsulated in silica sol–gel</atitle><jtitle>International journal of hydrogen energy</jtitle><date>2009</date><risdate>2009</risdate><volume>34</volume><issue>1</issue><spage>204</spage><epage>215</epage><pages>204-215</pages><issn>0360-3199</issn><eissn>1879-3487</eissn><coden>IJHEDX</coden><abstract>Hydrogen production was measured from viable wild-type and M55 mutant cells of
Synechocystis sp. PCC 6803 encapsulated in silica sol–gel derived from tetraethoxysilane, tetramethoxysilane, and a mixture of tetraethoxysilane with methyltriethoxysilane. Glycerol and polyethylene glycol were used as additives in concentrations up to 14
wt.% to increase porosity and pore connectivity and to reduce stress on the encapsulated cells. Mixtures were evaluated for hydrogen production in a high-throughput screening assay, and samples were imaged with environmental scanning electron microscopy. Compositions showing improved H
2 production were selected for further study via quantitative analysis of H
2 production in GC vial assays. H
2 production activity was monitored for up to 5 days and H
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| identifier | ISSN: 0360-3199 |
| ispartof | International journal of hydrogen energy, 2009, Vol.34 (1), p.204-215 |
| issn | 0360-3199 1879-3487 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_901670346 |
| source | Elsevier ScienceDirect Journals |
| subjects | Alternative fuels. Production and utilization Applied sciences Biophotolysis Biosolar Cyanobacteria Energy Exact sciences and technology Fuels Glycerol Hydrogen Hydrogenase Polyethylene glycol Synechocystis Tetraethoxysilane Tetramethoxysilane |
| title | Photobiological hydrogen production from Synechocystis sp. PCC 6803 encapsulated in silica sol–gel |
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