Oxidative desorption of thiocholine assembled on core-shell Fe3O4/AuNPs magnetic nanocomposites for highly sensitive determination of acetylcholinesterase activity: An exposure biomarker of organophosphates
Acetylcholinesterase (AChE) activity is a well established biomarker for biomonitoring of exposures to organophosphates (OPs) pesticides and chemical nerve agents. In this work, we described a novel electrochemical oxidative desorption-process of thiocholine, the product of enzymatic reaction, for r...
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| Veröffentlicht in: | Biosensors & bioelectronics 2011-06, Vol.26 (10), p.4231-4235 |
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| creator | Du, Dan Tao, Yuan Zhang, Weiying Liu, Deli Li, Haibing |
| description | Acetylcholinesterase (AChE) activity is a well established biomarker for biomonitoring of exposures to organophosphates (OPs) pesticides and chemical nerve agents. In this work, we described a novel electrochemical oxidative desorption-process of thiocholine, the product of enzymatic reaction, for rapid and highly sensitive determination of AChE activity in human serum. This principle is based on self-assembling of produced thiocholine onto core-shell Fe(3)O(4)/Au nanoparticles (Fe(3)O(4)/AuNPs) magnetic nanocomposites and its oxidation at electrode surface. Fe(3)O(4) magnetic core is not only used for magnetic separation from sample solutions, but also carrying more AuNPs due to its large surface-to-volume ratio. The core-shell Fe(3)O(4)/AuNPs nanocomposites were characterized by UV-Vis spectroscopy, field-emission scanning electron microscopy (FE-SEM) and electrochemical measurements. A linear relationship was obtained between the AChE activity and its concentration from 0.05 to 5.0 mU mL(-1) with a detection limit of 0.02 mU mL(-1). The method showed good results for characterization of AChE spiked human serum and detection of OP exposures from 0.05 to 20 nM, with detection limit of 0.02 nM. This new oxidative desorption assay thus provides a sensitive and quantitative tool for biomonitoring of the exposure to OP pesticides and nerve agents. |
| doi_str_mv | 10.1016/j.bios.2011.03.037 |
| format | Article |
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In this work, we described a novel electrochemical oxidative desorption-process of thiocholine, the product of enzymatic reaction, for rapid and highly sensitive determination of AChE activity in human serum. This principle is based on self-assembling of produced thiocholine onto core-shell Fe(3)O(4)/Au nanoparticles (Fe(3)O(4)/AuNPs) magnetic nanocomposites and its oxidation at electrode surface. Fe(3)O(4) magnetic core is not only used for magnetic separation from sample solutions, but also carrying more AuNPs due to its large surface-to-volume ratio. The core-shell Fe(3)O(4)/AuNPs nanocomposites were characterized by UV-Vis spectroscopy, field-emission scanning electron microscopy (FE-SEM) and electrochemical measurements. A linear relationship was obtained between the AChE activity and its concentration from 0.05 to 5.0 mU mL(-1) with a detection limit of 0.02 mU mL(-1). The method showed good results for characterization of AChE spiked human serum and detection of OP exposures from 0.05 to 20 nM, with detection limit of 0.02 nM. This new oxidative desorption assay thus provides a sensitive and quantitative tool for biomonitoring of the exposure to OP pesticides and nerve agents.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2011.03.037</identifier><identifier>PMID: 21514816</identifier><language>eng</language><publisher>Kidlington: Elsevier</publisher><subject>Acetylcholinesterase - analysis ; Biological and medical sciences ; Biomarkers - analysis ; Biosensing Techniques - instrumentation ; Biosensing Techniques - methods ; Biosensing Techniques - statistics & numerical data ; Biotechnology ; Electrochemical Techniques ; Environmental Monitoring - methods ; Fundamental and applied biological sciences. Psychology ; Gold ; Humans ; Magnetite Nanoparticles - ultrastructure ; Metal Nanoparticles - ultrastructure ; Microscopy, Electron, Scanning ; Nanocomposites - ultrastructure ; Organophosphates - administration & dosage ; Organophosphates - toxicity ; Oxidation-Reduction ; Sensitivity and Specificity ; Thiocholine</subject><ispartof>Biosensors & bioelectronics, 2011-06, Vol.26 (10), p.4231-4235</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-f325cb3d98f686ff69dc2a28b8a00d230a9622714a429a343ce8a16cce7de0ce3</citedby><cites>FETCH-LOGICAL-c430t-f325cb3d98f686ff69dc2a28b8a00d230a9622714a429a343ce8a16cce7de0ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24234165$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21514816$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Du, Dan</creatorcontrib><creatorcontrib>Tao, Yuan</creatorcontrib><creatorcontrib>Zhang, Weiying</creatorcontrib><creatorcontrib>Liu, Deli</creatorcontrib><creatorcontrib>Li, Haibing</creatorcontrib><title>Oxidative desorption of thiocholine assembled on core-shell Fe3O4/AuNPs magnetic nanocomposites for highly sensitive determination of acetylcholinesterase activity: An exposure biomarker of organophosphates</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>Acetylcholinesterase (AChE) activity is a well established biomarker for biomonitoring of exposures to organophosphates (OPs) pesticides and chemical nerve agents. In this work, we described a novel electrochemical oxidative desorption-process of thiocholine, the product of enzymatic reaction, for rapid and highly sensitive determination of AChE activity in human serum. This principle is based on self-assembling of produced thiocholine onto core-shell Fe(3)O(4)/Au nanoparticles (Fe(3)O(4)/AuNPs) magnetic nanocomposites and its oxidation at electrode surface. Fe(3)O(4) magnetic core is not only used for magnetic separation from sample solutions, but also carrying more AuNPs due to its large surface-to-volume ratio. The core-shell Fe(3)O(4)/AuNPs nanocomposites were characterized by UV-Vis spectroscopy, field-emission scanning electron microscopy (FE-SEM) and electrochemical measurements. A linear relationship was obtained between the AChE activity and its concentration from 0.05 to 5.0 mU mL(-1) with a detection limit of 0.02 mU mL(-1). The method showed good results for characterization of AChE spiked human serum and detection of OP exposures from 0.05 to 20 nM, with detection limit of 0.02 nM. This new oxidative desorption assay thus provides a sensitive and quantitative tool for biomonitoring of the exposure to OP pesticides and nerve agents.</description><subject>Acetylcholinesterase - analysis</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensing Techniques - statistics & numerical data</subject><subject>Biotechnology</subject><subject>Electrochemical Techniques</subject><subject>Environmental Monitoring - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gold</subject><subject>Humans</subject><subject>Magnetite Nanoparticles - ultrastructure</subject><subject>Metal Nanoparticles - ultrastructure</subject><subject>Microscopy, Electron, Scanning</subject><subject>Nanocomposites - ultrastructure</subject><subject>Organophosphates - administration & dosage</subject><subject>Organophosphates - toxicity</subject><subject>Oxidation-Reduction</subject><subject>Sensitivity and Specificity</subject><subject>Thiocholine</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks-O0zAQxiMEYsvCC3BAviBO6fpfnIRbtWIBaUU5wDmaOpPGJbGDJ0Hbl-SZcNUuHJFGsuT5zXwz_pxlrwVfCy7MzWG9c4HWkgux5ipF-SRbiapUuZaqeJqteF2YvDBGXWUviA6c81LU_Hl2JUUhdCXMKvu9fXAtzO4XshYpxGl2wbPQsbl3wfZhcB4ZEOG4G7BlKWdDxJx6HAZ2h2qrbzbLl6_ERth7nJ1lHnywYZwCuRmJdSGy3u374cgIfbo7S80YR-fhUQ0szsfhokcpCZRkbYLdfHzPNp7hQ-q4RGRp5xHiD4ynuhD3SW7qA009JLmX2bMOBsJXl_M6-3734dvtp_x--_Hz7eY-t1rxOe-ULOxOtXXVmcp0nalbK0FWuwo4b6XiUBspS6FByxqUVhYrEMZaLFvkFtV19u7cd4rh55ImbkZHNr0JeAwLNXXyx3BlxH_JytSaa12rRMozaWMgitg1U3Rp1WMjeHMyvDk0J8Obk-ENVynKVPTm0n7Zjdj-LXl0OAFvLwCQhaGL4K2jf1z6KlqYQv0BAhm7rQ</recordid><startdate>20110615</startdate><enddate>20110615</enddate><creator>Du, Dan</creator><creator>Tao, Yuan</creator><creator>Zhang, Weiying</creator><creator>Liu, Deli</creator><creator>Li, Haibing</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20110615</creationdate><title>Oxidative desorption of thiocholine assembled on core-shell Fe3O4/AuNPs magnetic nanocomposites for highly sensitive determination of acetylcholinesterase activity: An exposure biomarker of organophosphates</title><author>Du, Dan ; Tao, Yuan ; Zhang, Weiying ; Liu, Deli ; Li, Haibing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-f325cb3d98f686ff69dc2a28b8a00d230a9622714a429a343ce8a16cce7de0ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Acetylcholinesterase - analysis</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensing Techniques - statistics & numerical data</topic><topic>Biotechnology</topic><topic>Electrochemical Techniques</topic><topic>Environmental Monitoring - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gold</topic><topic>Humans</topic><topic>Magnetite Nanoparticles - ultrastructure</topic><topic>Metal Nanoparticles - ultrastructure</topic><topic>Microscopy, Electron, Scanning</topic><topic>Nanocomposites - ultrastructure</topic><topic>Organophosphates - administration & dosage</topic><topic>Organophosphates - toxicity</topic><topic>Oxidation-Reduction</topic><topic>Sensitivity and Specificity</topic><topic>Thiocholine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Du, Dan</creatorcontrib><creatorcontrib>Tao, Yuan</creatorcontrib><creatorcontrib>Zhang, Weiying</creatorcontrib><creatorcontrib>Liu, Deli</creatorcontrib><creatorcontrib>Li, Haibing</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Dan</au><au>Tao, Yuan</au><au>Zhang, Weiying</au><au>Liu, Deli</au><au>Li, Haibing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oxidative desorption of thiocholine assembled on core-shell Fe3O4/AuNPs magnetic nanocomposites for highly sensitive determination of acetylcholinesterase activity: An exposure biomarker of organophosphates</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2011-06-15</date><risdate>2011</risdate><volume>26</volume><issue>10</issue><spage>4231</spage><epage>4235</epage><pages>4231-4235</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Acetylcholinesterase (AChE) activity is a well established biomarker for biomonitoring of exposures to organophosphates (OPs) pesticides and chemical nerve agents. In this work, we described a novel electrochemical oxidative desorption-process of thiocholine, the product of enzymatic reaction, for rapid and highly sensitive determination of AChE activity in human serum. This principle is based on self-assembling of produced thiocholine onto core-shell Fe(3)O(4)/Au nanoparticles (Fe(3)O(4)/AuNPs) magnetic nanocomposites and its oxidation at electrode surface. Fe(3)O(4) magnetic core is not only used for magnetic separation from sample solutions, but also carrying more AuNPs due to its large surface-to-volume ratio. The core-shell Fe(3)O(4)/AuNPs nanocomposites were characterized by UV-Vis spectroscopy, field-emission scanning electron microscopy (FE-SEM) and electrochemical measurements. A linear relationship was obtained between the AChE activity and its concentration from 0.05 to 5.0 mU mL(-1) with a detection limit of 0.02 mU mL(-1). The method showed good results for characterization of AChE spiked human serum and detection of OP exposures from 0.05 to 20 nM, with detection limit of 0.02 nM. This new oxidative desorption assay thus provides a sensitive and quantitative tool for biomonitoring of the exposure to OP pesticides and nerve agents.</abstract><cop>Kidlington</cop><pub>Elsevier</pub><pmid>21514816</pmid><doi>10.1016/j.bios.2011.03.037</doi><tpages>5</tpages></addata></record> |
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| subjects | Acetylcholinesterase - analysis Biological and medical sciences Biomarkers - analysis Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensing Techniques - statistics & numerical data Biotechnology Electrochemical Techniques Environmental Monitoring - methods Fundamental and applied biological sciences. Psychology Gold Humans Magnetite Nanoparticles - ultrastructure Metal Nanoparticles - ultrastructure Microscopy, Electron, Scanning Nanocomposites - ultrastructure Organophosphates - administration & dosage Organophosphates - toxicity Oxidation-Reduction Sensitivity and Specificity Thiocholine |
| title | Oxidative desorption of thiocholine assembled on core-shell Fe3O4/AuNPs magnetic nanocomposites for highly sensitive determination of acetylcholinesterase activity: An exposure biomarker of organophosphates |
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