Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles
A simple, cost-effective and rapid colorimetric method for any or all of Hg 2+, Pb 2+ and Cu 2+ detection using papain-functionalized gold nanoparticles (P-AuNPs) has been developed. Papain is a protein with seven cystein residues, which can selectively bind with Hg 2+, Pb 2+ and Cu 2+. We functiona...
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| Veröffentlicht in: | Biosensors & bioelectronics 2011-06, Vol.26 (10), p.4064-4069 |
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| creator | Guo, Yongming Wang, Zhuo Qu, Weisi Shao, Huawu Jiang, Xingyu |
| description | A simple, cost-effective and rapid colorimetric method for any or all of Hg
2+, Pb
2+ and Cu
2+ detection using papain-functionalized gold nanoparticles (P-AuNPs) has been developed. Papain is a protein with seven cystein residues, which can selectively bind with Hg
2+, Pb
2+ and Cu
2+. We functionalized gold nanoparticles with papain. The P-AuNPs could be used to simultaneously detect Hg
2+, Pb
2+ and Cu
2+, and showed different responses to the three ions in an aqueous solution based on the aggregation-induced color change of gold nanoparticles. The P-AuNPs displayed the most obvious response to mercury ions in water in contrast to lead and copper ions, and the real water sample analysis verified the conclusion. The sensitivity of the detection system was influenced by the pH of the P-AuNPs solution, the concentration of P-AuNPs and the size of gold nanoparticles, and we found that larger gold nanoparticles contributed to more sensitive results. The detection system can detect as low as 200
nM Hg
2+, Pb
2+ or Cu
2+ using 42
nm gold nanoparticles. We expect our approach to have wide-ranging applications in the developing region for monitoring water quality in some areas. |
| doi_str_mv | 10.1016/j.bios.2011.03.033 |
| format | Article |
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2+, Pb
2+ and Cu
2+ detection using papain-functionalized gold nanoparticles (P-AuNPs) has been developed. Papain is a protein with seven cystein residues, which can selectively bind with Hg
2+, Pb
2+ and Cu
2+. We functionalized gold nanoparticles with papain. The P-AuNPs could be used to simultaneously detect Hg
2+, Pb
2+ and Cu
2+, and showed different responses to the three ions in an aqueous solution based on the aggregation-induced color change of gold nanoparticles. The P-AuNPs displayed the most obvious response to mercury ions in water in contrast to lead and copper ions, and the real water sample analysis verified the conclusion. The sensitivity of the detection system was influenced by the pH of the P-AuNPs solution, the concentration of P-AuNPs and the size of gold nanoparticles, and we found that larger gold nanoparticles contributed to more sensitive results. The detection system can detect as low as 200
nM Hg
2+, Pb
2+ or Cu
2+ using 42
nm gold nanoparticles. We expect our approach to have wide-ranging applications in the developing region for monitoring water quality in some areas.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2011.03.033</identifier><identifier>PMID: 21543219</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biosensing Techniques - methods ; Biotechnology ; Colorimetric detection ; Colorimetry ; Colorimetry - methods ; Copper ; Copper - analysis ; Copper ions ; Fundamental and applied biological sciences. Psychology ; Gold ; Gold nanoparticles ; Hydrogen-Ion Concentration ; Lead - analysis ; Lead ions ; Mercury ; Mercury - analysis ; Mercury ions ; Metal Nanoparticles - ultrastructure ; Monitoring ; Nanoparticles ; Papain ; Particle Size ; Protein ; Water Pollutants, Chemical - analysis</subject><ispartof>Biosensors & bioelectronics, 2011-06, Vol.26 (10), p.4064-4069</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-f12f9a3908dc3bfef83dbf0b7c2a265228abe04416f02680401aa7350f7f09e13</citedby><cites>FETCH-LOGICAL-c483t-f12f9a3908dc3bfef83dbf0b7c2a265228abe04416f02680401aa7350f7f09e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956566311001898$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24234138$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21543219$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Yongming</creatorcontrib><creatorcontrib>Wang, Zhuo</creatorcontrib><creatorcontrib>Qu, Weisi</creatorcontrib><creatorcontrib>Shao, Huawu</creatorcontrib><creatorcontrib>Jiang, Xingyu</creatorcontrib><title>Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>A simple, cost-effective and rapid colorimetric method for any or all of Hg
2+, Pb
2+ and Cu
2+ detection using papain-functionalized gold nanoparticles (P-AuNPs) has been developed. Papain is a protein with seven cystein residues, which can selectively bind with Hg
2+, Pb
2+ and Cu
2+. We functionalized gold nanoparticles with papain. The P-AuNPs could be used to simultaneously detect Hg
2+, Pb
2+ and Cu
2+, and showed different responses to the three ions in an aqueous solution based on the aggregation-induced color change of gold nanoparticles. The P-AuNPs displayed the most obvious response to mercury ions in water in contrast to lead and copper ions, and the real water sample analysis verified the conclusion. The sensitivity of the detection system was influenced by the pH of the P-AuNPs solution, the concentration of P-AuNPs and the size of gold nanoparticles, and we found that larger gold nanoparticles contributed to more sensitive results. The detection system can detect as low as 200
nM Hg
2+, Pb
2+ or Cu
2+ using 42
nm gold nanoparticles. We expect our approach to have wide-ranging applications in the developing region for monitoring water quality in some areas.</description><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>Biotechnology</subject><subject>Colorimetric detection</subject><subject>Colorimetry</subject><subject>Colorimetry - methods</subject><subject>Copper</subject><subject>Copper - analysis</subject><subject>Copper ions</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gold</subject><subject>Gold nanoparticles</subject><subject>Hydrogen-Ion Concentration</subject><subject>Lead - analysis</subject><subject>Lead ions</subject><subject>Mercury</subject><subject>Mercury - analysis</subject><subject>Mercury ions</subject><subject>Metal Nanoparticles - ultrastructure</subject><subject>Monitoring</subject><subject>Nanoparticles</subject><subject>Papain</subject><subject>Particle Size</subject><subject>Protein</subject><subject>Water Pollutants, Chemical - analysis</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoO4uLOrf8CD5CJ7scdK0p_gRYZ1XVjwoueQTipLhnTSJt0L468344x6UyjIIU-9FO9DyGsGWwasfb_fji7mLQfGtiDKiGdkw_pOVDUXzXOygaFpq6ZtxSW5ynkPAB0b4AW55KypBWfDhjztoo_JTbgkp6nBBfXiYqDR0gmTXtPhHfWoDFXBUB3nGRMt_5lmN61-UQHjmv2BrtmFRzqnuKALlV3Drxjl3Q809DF6Q4MKcVZpcdpjfkkurPIZX53fa_Lt0-3X3efq4cvd_e7jQ6XrXiyVZdwOSgzQGy1Gi7YXZrQwdpor3jac92pEqGvWWuBtDzUwpTrRgO0sDMjENbk55ZbLvq-YFzm5rNH70-FyKD02pZbuv2TfDjW0AqCQ_ETqFHNOaOVcClTpIBnIoxi5l0cx8ihGgigjytKbc_w6Tmj-rPw2UYC3Z0BlrbxNKmiX_3JFac1EX7gPJw5LbU8Ok8zaYdBoXCrupInuX3f8BBhTrks</recordid><startdate>20110615</startdate><enddate>20110615</enddate><creator>Guo, Yongming</creator><creator>Wang, Zhuo</creator><creator>Qu, Weisi</creator><creator>Shao, Huawu</creator><creator>Jiang, Xingyu</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope></search><sort><creationdate>20110615</creationdate><title>Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles</title><author>Guo, Yongming ; Wang, Zhuo ; Qu, Weisi ; Shao, Huawu ; Jiang, Xingyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-f12f9a3908dc3bfef83dbf0b7c2a265228abe04416f02680401aa7350f7f09e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>Biotechnology</topic><topic>Colorimetric detection</topic><topic>Colorimetry</topic><topic>Colorimetry - methods</topic><topic>Copper</topic><topic>Copper - analysis</topic><topic>Copper ions</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gold</topic><topic>Gold nanoparticles</topic><topic>Hydrogen-Ion Concentration</topic><topic>Lead - analysis</topic><topic>Lead ions</topic><topic>Mercury</topic><topic>Mercury - analysis</topic><topic>Mercury ions</topic><topic>Metal Nanoparticles - ultrastructure</topic><topic>Monitoring</topic><topic>Nanoparticles</topic><topic>Papain</topic><topic>Particle Size</topic><topic>Protein</topic><topic>Water Pollutants, Chemical - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Yongming</creatorcontrib><creatorcontrib>Wang, Zhuo</creatorcontrib><creatorcontrib>Qu, Weisi</creatorcontrib><creatorcontrib>Shao, Huawu</creatorcontrib><creatorcontrib>Jiang, Xingyu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Yongming</au><au>Wang, Zhuo</au><au>Qu, Weisi</au><au>Shao, Huawu</au><au>Jiang, Xingyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2011-06-15</date><risdate>2011</risdate><volume>26</volume><issue>10</issue><spage>4064</spage><epage>4069</epage><pages>4064-4069</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>A simple, cost-effective and rapid colorimetric method for any or all of Hg
2+, Pb
2+ and Cu
2+ detection using papain-functionalized gold nanoparticles (P-AuNPs) has been developed. Papain is a protein with seven cystein residues, which can selectively bind with Hg
2+, Pb
2+ and Cu
2+. We functionalized gold nanoparticles with papain. The P-AuNPs could be used to simultaneously detect Hg
2+, Pb
2+ and Cu
2+, and showed different responses to the three ions in an aqueous solution based on the aggregation-induced color change of gold nanoparticles. The P-AuNPs displayed the most obvious response to mercury ions in water in contrast to lead and copper ions, and the real water sample analysis verified the conclusion. The sensitivity of the detection system was influenced by the pH of the P-AuNPs solution, the concentration of P-AuNPs and the size of gold nanoparticles, and we found that larger gold nanoparticles contributed to more sensitive results. The detection system can detect as low as 200
nM Hg
2+, Pb
2+ or Cu
2+ using 42
nm gold nanoparticles. We expect our approach to have wide-ranging applications in the developing region for monitoring water quality in some areas.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>21543219</pmid><doi>10.1016/j.bios.2011.03.033</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biosensors & bioelectronics, 2011-06, Vol.26 (10), p.4064-4069 |
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| subjects | Biological and medical sciences Biosensing Techniques - methods Biotechnology Colorimetric detection Colorimetry Colorimetry - methods Copper Copper - analysis Copper ions Fundamental and applied biological sciences. Psychology Gold Gold nanoparticles Hydrogen-Ion Concentration Lead - analysis Lead ions Mercury Mercury - analysis Mercury ions Metal Nanoparticles - ultrastructure Monitoring Nanoparticles Papain Particle Size Protein Water Pollutants, Chemical - analysis |
| title | Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles |
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