Mitochondrial involvement in carbachol-induced intracellular Ca2+ mobilization and contraction in rat gastric smooth muscle
Mitochondria are important modulators of Ca2+ homeostasis. However, it is not clear if they modulate and participate in smooth muscle signaling and contraction. The aim of the present work was to investigate the role of mitochondria in Ca2+ transients and contraction induced by metabotropic muscarin...
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| Veröffentlicht in: | Life sciences (1973) 2011-11, Vol.89 (21-22), p.757-764 |
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| creator | Correa, Roberta M. Lafayette, Simone S.L. Pereira, Gustavo J.S. Hirata, Hanako Garcez-do-Carmo, Lúcia Smaili, Soraya S. |
| description | Mitochondria are important modulators of Ca2+ homeostasis. However, it is not clear if they modulate and participate in smooth muscle signaling and contraction. The aim of the present work was to investigate the role of mitochondria in Ca2+ transients and contraction induced by metabotropic muscarinic receptor activation in rat gastric smooth muscle.
Carbachol (CCh)-induced contraction was investigated in the absence or presence of increasing concentration of mitochondrial protonophore, carbonyl cyanide p-(trifluoro-methoxy)phenyl-hydrazone (FCCP), in gastric fundus strips. Ca2+ and mitochondrial membrane potential (ΔΨm) measurements were performed in primarily cultured gastric smooth muscle cells loaded with FURA-2 or TMRE dyes.
Results show that CCh (1μM)-induced contraction was inhibited by FCCP in a concentration-dependent manner. In cultured smooth muscle cells CCh (1μM) caused a cytosolic Ca2+ rise. Preincubation with FCCP strongly inhibited CCh-evoked Ca2+ transients indicating that mitochondria shape intracellular Ca2+ signals. CCh induced elevations of ∆Ψm in 60% of the individual mitochondrion analyzed.
Taken together our results indicate that CCh induces release of Ca2+ from intracellular stores, which may be modulated by mitochondria. Thus, mitochondria participate of the intracellular Ca2+ homeostasis in muscarinic contraction in gastric fundus smooth muscle. |
| doi_str_mv | 10.1016/j.lfs.2011.08.003 |
| format | Article |
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Carbachol (CCh)-induced contraction was investigated in the absence or presence of increasing concentration of mitochondrial protonophore, carbonyl cyanide p-(trifluoro-methoxy)phenyl-hydrazone (FCCP), in gastric fundus strips. Ca2+ and mitochondrial membrane potential (ΔΨm) measurements were performed in primarily cultured gastric smooth muscle cells loaded with FURA-2 or TMRE dyes.
Results show that CCh (1μM)-induced contraction was inhibited by FCCP in a concentration-dependent manner. In cultured smooth muscle cells CCh (1μM) caused a cytosolic Ca2+ rise. Preincubation with FCCP strongly inhibited CCh-evoked Ca2+ transients indicating that mitochondria shape intracellular Ca2+ signals. CCh induced elevations of ∆Ψm in 60% of the individual mitochondrion analyzed.
Taken together our results indicate that CCh induces release of Ca2+ from intracellular stores, which may be modulated by mitochondria. Thus, mitochondria participate of the intracellular Ca2+ homeostasis in muscarinic contraction in gastric fundus smooth muscle.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2011.08.003</identifier><identifier>PMID: 21871904</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Calcium ; Calcium - metabolism ; Calcium Signaling - drug effects ; Carbachol ; Carbachol - pharmacology ; Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology ; Cells, Cultured ; Contraction ; dyes ; gastric fundus ; homeostasis ; Immunohistochemistry ; In Vitro Techniques ; Male ; membrane potential ; Mitochondria ; Mitochondria, Muscle - drug effects ; Mitochondria, Muscle - metabolism ; mitochondrial membrane ; Muscle Contraction - drug effects ; Muscle, Smooth - drug effects ; Muscle, Smooth - metabolism ; myocytes ; Myocytes, Smooth Muscle - physiology ; Rats ; Rats, Wistar ; Receptors, Muscarinic - drug effects ; Sarcoplasmic Reticulum - drug effects ; Sarcoplasmic Reticulum - metabolism ; Smooth muscle ; Stomach - drug effects ; Stomach - metabolism ; Uncoupling Agents - pharmacology</subject><ispartof>Life sciences (1973), 2011-11, Vol.89 (21-22), p.757-764</ispartof><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-84f1ff441e36dcf898572f8879fa040222d2dbbd1c2d5f1f37098173dde876353</citedby><cites>FETCH-LOGICAL-c349t-84f1ff441e36dcf898572f8879fa040222d2dbbd1c2d5f1f37098173dde876353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.lfs.2011.08.003$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21871904$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Correa, Roberta M.</creatorcontrib><creatorcontrib>Lafayette, Simone S.L.</creatorcontrib><creatorcontrib>Pereira, Gustavo J.S.</creatorcontrib><creatorcontrib>Hirata, Hanako</creatorcontrib><creatorcontrib>Garcez-do-Carmo, Lúcia</creatorcontrib><creatorcontrib>Smaili, Soraya S.</creatorcontrib><title>Mitochondrial involvement in carbachol-induced intracellular Ca2+ mobilization and contraction in rat gastric smooth muscle</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Mitochondria are important modulators of Ca2+ homeostasis. However, it is not clear if they modulate and participate in smooth muscle signaling and contraction. The aim of the present work was to investigate the role of mitochondria in Ca2+ transients and contraction induced by metabotropic muscarinic receptor activation in rat gastric smooth muscle.
Carbachol (CCh)-induced contraction was investigated in the absence or presence of increasing concentration of mitochondrial protonophore, carbonyl cyanide p-(trifluoro-methoxy)phenyl-hydrazone (FCCP), in gastric fundus strips. Ca2+ and mitochondrial membrane potential (ΔΨm) measurements were performed in primarily cultured gastric smooth muscle cells loaded with FURA-2 or TMRE dyes.
Results show that CCh (1μM)-induced contraction was inhibited by FCCP in a concentration-dependent manner. In cultured smooth muscle cells CCh (1μM) caused a cytosolic Ca2+ rise. Preincubation with FCCP strongly inhibited CCh-evoked Ca2+ transients indicating that mitochondria shape intracellular Ca2+ signals. CCh induced elevations of ∆Ψm in 60% of the individual mitochondrion analyzed.
Taken together our results indicate that CCh induces release of Ca2+ from intracellular stores, which may be modulated by mitochondria. Thus, mitochondria participate of the intracellular Ca2+ homeostasis in muscarinic contraction in gastric fundus smooth muscle.</description><subject>Animals</subject><subject>Calcium</subject><subject>Calcium - metabolism</subject><subject>Calcium Signaling - drug effects</subject><subject>Carbachol</subject><subject>Carbachol - pharmacology</subject><subject>Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology</subject><subject>Cells, Cultured</subject><subject>Contraction</subject><subject>dyes</subject><subject>gastric fundus</subject><subject>homeostasis</subject><subject>Immunohistochemistry</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>membrane potential</subject><subject>Mitochondria</subject><subject>Mitochondria, Muscle - drug effects</subject><subject>Mitochondria, Muscle - metabolism</subject><subject>mitochondrial membrane</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - metabolism</subject><subject>myocytes</subject><subject>Myocytes, Smooth Muscle - physiology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Muscarinic - drug effects</subject><subject>Sarcoplasmic Reticulum - drug effects</subject><subject>Sarcoplasmic Reticulum - metabolism</subject><subject>Smooth muscle</subject><subject>Stomach - drug effects</subject><subject>Stomach - metabolism</subject><subject>Uncoupling Agents - pharmacology</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi0EotvCD-ACufWAEsZ2PhxxQqtSkFr1AD1bjj9ar5y42M5KwJ9ntls4crDssZ955XkIeUOhoUD7D7smuNwwoLQB0QDwZ2RDxTDW0HP6nGwAWFtzBt0JOc15BwBdN_CX5IQhRUdoN-T3tS9R38fFJK9C5Zd9DHs726XgudIqTQpfQ-0Xs2pr8LIkpW0Ia1Cp2ir2vprj5IP_pYqPS6UWU-n4CD3WGJJUqe5ULsnrKs8xlvtqXrMO9hV54VTI9vXTfkZuP198336pr24uv24_XdWat2OpReuoc21LLe-NdmIU3cCcwDmdghYYY4aZaTJUM9MhygcYBR24MVYMPe_4GTk_5j6k-GO1ucjZ58MMarFxzXJEly3jokeSHkmdYs7JOvmQ_KzST0lBHpTLnUTl8qBcgpCoHHvePqWv02zNv46_jhF4dwScilLdJZ_l7TdM6AAXHZlA4uORsGhh722SWXu7oG-frC7SRP-fD_wBLoKczg</recordid><startdate>20111121</startdate><enddate>20111121</enddate><creator>Correa, Roberta M.</creator><creator>Lafayette, Simone S.L.</creator><creator>Pereira, Gustavo J.S.</creator><creator>Hirata, Hanako</creator><creator>Garcez-do-Carmo, Lúcia</creator><creator>Smaili, Soraya S.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20111121</creationdate><title>Mitochondrial involvement in carbachol-induced intracellular Ca2+ mobilization and contraction in rat gastric smooth muscle</title><author>Correa, Roberta M. ; Lafayette, Simone S.L. ; Pereira, Gustavo J.S. ; Hirata, Hanako ; Garcez-do-Carmo, Lúcia ; Smaili, Soraya S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-84f1ff441e36dcf898572f8879fa040222d2dbbd1c2d5f1f37098173dde876353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Calcium</topic><topic>Calcium - metabolism</topic><topic>Calcium Signaling - drug effects</topic><topic>Carbachol</topic><topic>Carbachol - pharmacology</topic><topic>Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology</topic><topic>Cells, Cultured</topic><topic>Contraction</topic><topic>dyes</topic><topic>gastric fundus</topic><topic>homeostasis</topic><topic>Immunohistochemistry</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>membrane potential</topic><topic>Mitochondria</topic><topic>Mitochondria, Muscle - drug effects</topic><topic>Mitochondria, Muscle - metabolism</topic><topic>mitochondrial membrane</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - metabolism</topic><topic>myocytes</topic><topic>Myocytes, Smooth Muscle - physiology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Muscarinic - drug effects</topic><topic>Sarcoplasmic Reticulum - drug effects</topic><topic>Sarcoplasmic Reticulum - metabolism</topic><topic>Smooth muscle</topic><topic>Stomach - drug effects</topic><topic>Stomach - metabolism</topic><topic>Uncoupling Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Correa, Roberta M.</creatorcontrib><creatorcontrib>Lafayette, Simone S.L.</creatorcontrib><creatorcontrib>Pereira, Gustavo J.S.</creatorcontrib><creatorcontrib>Hirata, Hanako</creatorcontrib><creatorcontrib>Garcez-do-Carmo, Lúcia</creatorcontrib><creatorcontrib>Smaili, Soraya S.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Correa, Roberta M.</au><au>Lafayette, Simone S.L.</au><au>Pereira, Gustavo J.S.</au><au>Hirata, Hanako</au><au>Garcez-do-Carmo, Lúcia</au><au>Smaili, Soraya S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mitochondrial involvement in carbachol-induced intracellular Ca2+ mobilization and contraction in rat gastric smooth muscle</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2011-11-21</date><risdate>2011</risdate><volume>89</volume><issue>21-22</issue><spage>757</spage><epage>764</epage><pages>757-764</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Mitochondria are important modulators of Ca2+ homeostasis. However, it is not clear if they modulate and participate in smooth muscle signaling and contraction. The aim of the present work was to investigate the role of mitochondria in Ca2+ transients and contraction induced by metabotropic muscarinic receptor activation in rat gastric smooth muscle.
Carbachol (CCh)-induced contraction was investigated in the absence or presence of increasing concentration of mitochondrial protonophore, carbonyl cyanide p-(trifluoro-methoxy)phenyl-hydrazone (FCCP), in gastric fundus strips. Ca2+ and mitochondrial membrane potential (ΔΨm) measurements were performed in primarily cultured gastric smooth muscle cells loaded with FURA-2 or TMRE dyes.
Results show that CCh (1μM)-induced contraction was inhibited by FCCP in a concentration-dependent manner. In cultured smooth muscle cells CCh (1μM) caused a cytosolic Ca2+ rise. Preincubation with FCCP strongly inhibited CCh-evoked Ca2+ transients indicating that mitochondria shape intracellular Ca2+ signals. CCh induced elevations of ∆Ψm in 60% of the individual mitochondrion analyzed.
Taken together our results indicate that CCh induces release of Ca2+ from intracellular stores, which may be modulated by mitochondria. Thus, mitochondria participate of the intracellular Ca2+ homeostasis in muscarinic contraction in gastric fundus smooth muscle.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>21871904</pmid><doi>10.1016/j.lfs.2011.08.003</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Calcium Calcium - metabolism Calcium Signaling - drug effects Carbachol Carbachol - pharmacology Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology Cells, Cultured Contraction dyes gastric fundus homeostasis Immunohistochemistry In Vitro Techniques Male membrane potential Mitochondria Mitochondria, Muscle - drug effects Mitochondria, Muscle - metabolism mitochondrial membrane Muscle Contraction - drug effects Muscle, Smooth - drug effects Muscle, Smooth - metabolism myocytes Myocytes, Smooth Muscle - physiology Rats Rats, Wistar Receptors, Muscarinic - drug effects Sarcoplasmic Reticulum - drug effects Sarcoplasmic Reticulum - metabolism Smooth muscle Stomach - drug effects Stomach - metabolism Uncoupling Agents - pharmacology |
| title | Mitochondrial involvement in carbachol-induced intracellular Ca2+ mobilization and contraction in rat gastric smooth muscle |
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