Effects of (-)-epigallocatechin-3-O-gallate on expression of gluconeogenesis-related genes in the mouse duodenum
Green tea has been shown to have many beneficial health effects. We have previously reported that dietary (-)-epigallocatechin-3-O-gallate (EGCG), the major polyphenol in green tea, reduced gene expressions of gluconeogenic enzymes, glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinas...
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| Veröffentlicht in: | Biomedical research (Tokyo) 2011-10, Vol.32 (5), p.313-320 |
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| creator | Yasui, Kensuke Tanabe, Hiroki Miyoshi, Noriyuki Suzuki, Takuji Goto, Shingo Taguchi, Kyoko Ishigami, Yoko Paeng, Noriko Fukutomi, Ryuuta Imai, Shinjiro Isemura, Mamoru |
| description | Green tea has been shown to have many beneficial health effects. We have previously reported that dietary (-)-epigallocatechin-3-O-gallate (EGCG), the major polyphenol in green tea, reduced gene expressions of gluconeogenic enzymes, glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK), in the normal mouse liver. In the present study, we examined the effects of intragastrical administration of EGCG on the expression of gluconeogenesis-related genes in the mouse intestine. The results of experiments with the semi-quantitative reverse transcription-polymerase chain reaction indicated that EGCG at 0.6 mg/head caused a reduced expression of G6Pase, PEPCK, hepatocyte nuclear factor 1α (HNF1α), and HNF4α. Experiments using the quantitative real-time polymerase chain reaction confirmed these effects. We then examined the effects of EGCG using human colon carcinoma Caco-2 cells stimulated with dexamethasone and dibutyryl cAMP. The results were generally consistent with those from the experiments in vivo. The present findings suggest EGCG to contribute to the beneficial effects of green tea on diabetes, obesity, and cancer by modulating gene expression in the intestine. |
| doi_str_mv | 10.2220/biomedres.32.313 |
| format | Article |
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We have previously reported that dietary (-)-epigallocatechin-3-O-gallate (EGCG), the major polyphenol in green tea, reduced gene expressions of gluconeogenic enzymes, glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK), in the normal mouse liver. In the present study, we examined the effects of intragastrical administration of EGCG on the expression of gluconeogenesis-related genes in the mouse intestine. The results of experiments with the semi-quantitative reverse transcription-polymerase chain reaction indicated that EGCG at 0.6 mg/head caused a reduced expression of G6Pase, PEPCK, hepatocyte nuclear factor 1α (HNF1α), and HNF4α. Experiments using the quantitative real-time polymerase chain reaction confirmed these effects. We then examined the effects of EGCG using human colon carcinoma Caco-2 cells stimulated with dexamethasone and dibutyryl cAMP. The results were generally consistent with those from the experiments in vivo. 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We have previously reported that dietary (-)-epigallocatechin-3-O-gallate (EGCG), the major polyphenol in green tea, reduced gene expressions of gluconeogenic enzymes, glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK), in the normal mouse liver. In the present study, we examined the effects of intragastrical administration of EGCG on the expression of gluconeogenesis-related genes in the mouse intestine. The results of experiments with the semi-quantitative reverse transcription-polymerase chain reaction indicated that EGCG at 0.6 mg/head caused a reduced expression of G6Pase, PEPCK, hepatocyte nuclear factor 1α (HNF1α), and HNF4α. Experiments using the quantitative real-time polymerase chain reaction confirmed these effects. We then examined the effects of EGCG using human colon carcinoma Caco-2 cells stimulated with dexamethasone and dibutyryl cAMP. The results were generally consistent with those from the experiments in vivo. The present findings suggest EGCG to contribute to the beneficial effects of green tea on diabetes, obesity, and cancer by modulating gene expression in the intestine.</description><subject>Acetylcysteine - pharmacology</subject><subject>Animals</subject><subject>Antioxidants - administration & dosage</subject><subject>Antioxidants - pharmacology</subject><subject>Catechin - administration & dosage</subject><subject>Catechin - analogs & derivatives</subject><subject>Catechin - pharmacology</subject><subject>Cell Line, Tumor</subject><subject>Colon</subject><subject>Cyclic AMP</subject><subject>Dexamethasone</subject><subject>Diabetes mellitus</subject><subject>Dose-Response Relationship, Drug</subject><subject>Duodenum</subject><subject>Duodenum - drug effects</subject><subject>Duodenum - metabolism</subject><subject>Enzymes</subject><subject>epigallocatechin-3-gallate</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Gluconeogenesis - drug effects</subject><subject>Gluconeogenesis - genetics</subject><subject>Glucose-6-phosphatase</subject><subject>green tea</subject><subject>Hepatocytes</subject><subject>Humans</subject><subject>Intestine</subject><subject>Liver</subject><subject>Mice</subject><subject>Obesity</subject><subject>Polymerase chain reaction</subject><subject>Polyphenols</subject><issn>0388-6107</issn><issn>1880-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UD1PwzAUtBCIlsLOhLxRBhd_JLE9oqp8SJW6gMQWJfZL6yqJQ5xI8O9xoaxM7-7p7n0cQteMLjjn9L50vgHbQ1gIvhBMnKApU4qSCN9P0ZQKpUjGqJygixD2NHKmxDmaRK8QgtIp6lZVBWYI2Fd4Tu4IdG5b1LU3xQBm51oiyIYcOpFj32L47OK64CKMjm09Gt-C30ILwQXSw0Fn8Q_HrsXDDnDjxwDYjt5COzaX6Kwq6gBXxzpDb4-r1-UzWW-eXpYPa7JnWg4kK1KWauCpKYU2OpWGV5KCtMCYtaxIs0IpaVUJieaKW51pKiFRSiWy4mDEDN3-zu16_zFCGPLGBQPxkXjvGHJNGaVpluqonP-rZFSLJM1ozGyGbo7SsYzB513vmqL_yv_yFN8Ydnj4</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Yasui, Kensuke</creator><creator>Tanabe, Hiroki</creator><creator>Miyoshi, Noriyuki</creator><creator>Suzuki, Takuji</creator><creator>Goto, Shingo</creator><creator>Taguchi, Kyoko</creator><creator>Ishigami, Yoko</creator><creator>Paeng, Noriko</creator><creator>Fukutomi, Ryuuta</creator><creator>Imai, Shinjiro</creator><creator>Isemura, Mamoru</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20111001</creationdate><title>Effects of (-)-epigallocatechin-3-O-gallate on expression of gluconeogenesis-related genes in the mouse duodenum</title><author>Yasui, Kensuke ; Tanabe, Hiroki ; Miyoshi, Noriyuki ; Suzuki, Takuji ; Goto, Shingo ; Taguchi, Kyoko ; Ishigami, Yoko ; Paeng, Noriko ; Fukutomi, Ryuuta ; Imai, Shinjiro ; Isemura, Mamoru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j197t-6a5159e25cb39c957c2f70e7de11dd1a56a887d8be49282d96907e488847f2ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Acetylcysteine - pharmacology</topic><topic>Animals</topic><topic>Antioxidants - administration & dosage</topic><topic>Antioxidants - pharmacology</topic><topic>Catechin - administration & dosage</topic><topic>Catechin - analogs & derivatives</topic><topic>Catechin - pharmacology</topic><topic>Cell Line, Tumor</topic><topic>Colon</topic><topic>Cyclic AMP</topic><topic>Dexamethasone</topic><topic>Diabetes mellitus</topic><topic>Dose-Response Relationship, Drug</topic><topic>Duodenum</topic><topic>Duodenum - drug effects</topic><topic>Duodenum - metabolism</topic><topic>Enzymes</topic><topic>epigallocatechin-3-gallate</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Gluconeogenesis - drug effects</topic><topic>Gluconeogenesis - genetics</topic><topic>Glucose-6-phosphatase</topic><topic>green tea</topic><topic>Hepatocytes</topic><topic>Humans</topic><topic>Intestine</topic><topic>Liver</topic><topic>Mice</topic><topic>Obesity</topic><topic>Polymerase chain reaction</topic><topic>Polyphenols</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yasui, Kensuke</creatorcontrib><creatorcontrib>Tanabe, Hiroki</creatorcontrib><creatorcontrib>Miyoshi, Noriyuki</creatorcontrib><creatorcontrib>Suzuki, Takuji</creatorcontrib><creatorcontrib>Goto, Shingo</creatorcontrib><creatorcontrib>Taguchi, Kyoko</creatorcontrib><creatorcontrib>Ishigami, Yoko</creatorcontrib><creatorcontrib>Paeng, Noriko</creatorcontrib><creatorcontrib>Fukutomi, Ryuuta</creatorcontrib><creatorcontrib>Imai, Shinjiro</creatorcontrib><creatorcontrib>Isemura, Mamoru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical research (Tokyo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yasui, Kensuke</au><au>Tanabe, Hiroki</au><au>Miyoshi, Noriyuki</au><au>Suzuki, Takuji</au><au>Goto, Shingo</au><au>Taguchi, Kyoko</au><au>Ishigami, Yoko</au><au>Paeng, Noriko</au><au>Fukutomi, Ryuuta</au><au>Imai, Shinjiro</au><au>Isemura, Mamoru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of (-)-epigallocatechin-3-O-gallate on expression of gluconeogenesis-related genes in the mouse duodenum</atitle><jtitle>Biomedical research (Tokyo)</jtitle><addtitle>Biomed Res</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>32</volume><issue>5</issue><spage>313</spage><epage>320</epage><pages>313-320</pages><issn>0388-6107</issn><eissn>1880-313X</eissn><abstract>Green tea has been shown to have many beneficial health effects. We have previously reported that dietary (-)-epigallocatechin-3-O-gallate (EGCG), the major polyphenol in green tea, reduced gene expressions of gluconeogenic enzymes, glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK), in the normal mouse liver. In the present study, we examined the effects of intragastrical administration of EGCG on the expression of gluconeogenesis-related genes in the mouse intestine. The results of experiments with the semi-quantitative reverse transcription-polymerase chain reaction indicated that EGCG at 0.6 mg/head caused a reduced expression of G6Pase, PEPCK, hepatocyte nuclear factor 1α (HNF1α), and HNF4α. Experiments using the quantitative real-time polymerase chain reaction confirmed these effects. We then examined the effects of EGCG using human colon carcinoma Caco-2 cells stimulated with dexamethasone and dibutyryl cAMP. The results were generally consistent with those from the experiments in vivo. The present findings suggest EGCG to contribute to the beneficial effects of green tea on diabetes, obesity, and cancer by modulating gene expression in the intestine.</abstract><cop>Japan</cop><pmid>22033300</pmid><doi>10.2220/biomedres.32.313</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Acetylcysteine - pharmacology Animals Antioxidants - administration & dosage Antioxidants - pharmacology Catechin - administration & dosage Catechin - analogs & derivatives Catechin - pharmacology Cell Line, Tumor Colon Cyclic AMP Dexamethasone Diabetes mellitus Dose-Response Relationship, Drug Duodenum Duodenum - drug effects Duodenum - metabolism Enzymes epigallocatechin-3-gallate Gene Expression Regulation - drug effects Gluconeogenesis - drug effects Gluconeogenesis - genetics Glucose-6-phosphatase green tea Hepatocytes Humans Intestine Liver Mice Obesity Polymerase chain reaction Polyphenols |
| title | Effects of (-)-epigallocatechin-3-O-gallate on expression of gluconeogenesis-related genes in the mouse duodenum |
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