C-Reactive protein-directed immobilization of phosphocholine ligands on a solid surface
The complexes of C-reactive protein (CRP) with its polymerizable phosphocholine ligands adsorbed at the styrene-water interface were polymerized. The molecularly imprinted polymer (MIP) exhibited a binding affinity for CRP comparable to that of immobilized anti-CRP antibody. The determination of hum...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2011-11, Vol.47 (43), p.11900-11902 |
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| container_end_page | 11902 |
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| container_issue | 43 |
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| container_title | Chemical communications (Cambridge, England) |
| container_volume | 47 |
| creator | Kim, Eunjoo Kim, Hyun-Chul Lee, Se Geun Lee, Sung Jun Go, Tae-Jung Baek, Chul Su Jeong, Sang Won |
| description | The complexes of C-reactive protein (CRP) with its polymerizable phosphocholine ligands adsorbed at the styrene-water interface were polymerized. The molecularly imprinted polymer (MIP) exhibited a binding affinity for CRP comparable to that of immobilized anti-CRP antibody. The determination of human serum CRP using the MIP-based sandwich immunoassay has been demonstrated. |
| doi_str_mv | 10.1039/c1cc15079k |
| format | Article |
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The molecularly imprinted polymer (MIP) exhibited a binding affinity for CRP comparable to that of immobilized anti-CRP antibody. The determination of human serum CRP using the MIP-based sandwich immunoassay has been demonstrated.</description><identifier>ISSN: 1359-7345</identifier><identifier>EISSN: 1364-548X</identifier><identifier>DOI: 10.1039/c1cc15079k</identifier><identifier>PMID: 21975508</identifier><language>eng</language><publisher>England</publisher><subject>Antibodies, Immobilized - immunology ; C-Reactive Protein - analysis ; C-Reactive Protein - immunology ; Humans ; Immunoassay - methods ; Ligands ; Molecular Imprinting ; Oils - chemistry ; Phosphorylcholine - chemistry ; Polystyrenes - chemistry ; Surface Properties ; Water - chemistry</subject><ispartof>Chemical communications (Cambridge, England), 2011-11, Vol.47 (43), p.11900-11902</ispartof><rights>This journal is © The Royal Society of Chemistry 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-b11cbfe2de66038573390af7e2d4a5b14c74247d59ec52ea82ca173d3939cdc63</citedby><cites>FETCH-LOGICAL-c352t-b11cbfe2de66038573390af7e2d4a5b14c74247d59ec52ea82ca173d3939cdc63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21975508$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Eunjoo</creatorcontrib><creatorcontrib>Kim, Hyun-Chul</creatorcontrib><creatorcontrib>Lee, Se Geun</creatorcontrib><creatorcontrib>Lee, Sung Jun</creatorcontrib><creatorcontrib>Go, Tae-Jung</creatorcontrib><creatorcontrib>Baek, Chul Su</creatorcontrib><creatorcontrib>Jeong, Sang Won</creatorcontrib><title>C-Reactive protein-directed immobilization of phosphocholine ligands on a solid surface</title><title>Chemical communications (Cambridge, England)</title><addtitle>Chem Commun (Camb)</addtitle><description>The complexes of C-reactive protein (CRP) with its polymerizable phosphocholine ligands adsorbed at the styrene-water interface were polymerized. The molecularly imprinted polymer (MIP) exhibited a binding affinity for CRP comparable to that of immobilized anti-CRP antibody. The determination of human serum CRP using the MIP-based sandwich immunoassay has been demonstrated.</description><subject>Antibodies, Immobilized - immunology</subject><subject>C-Reactive Protein - analysis</subject><subject>C-Reactive Protein - immunology</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Ligands</subject><subject>Molecular Imprinting</subject><subject>Oils - chemistry</subject><subject>Phosphorylcholine - chemistry</subject><subject>Polystyrenes - chemistry</subject><subject>Surface Properties</subject><subject>Water - chemistry</subject><issn>1359-7345</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkN1KxDAQhYMo7rp64wNI7wShmjRJ01zK4h8sCKLoXUknUzfaNmvTCvr0ZtlVB4YZznwchkPIMaPnjHJ9AQyASar0-w6ZMp6LVIriZXe9S50qLuSEHITwRmMxWeyTSca0kpIWU_I8Tx_QwOA-MVn1fkDXpdb1CAPaxLWtr1zjvs3gfJf4OlktfYgNS9-4DpPGvZrOhiQeTRKiZpMw9rUBPCR7tWkCHm3njDxdXz3Ob9PF_c3d_HKRApfZkFaMQVVjZjHPKS-k4lxTU6uoCCMrJkCJTCgrNYLM0BQZGKa45ZprsJDzGTnd-MbnP0YMQ9m6ANg0pkM_hlJTmgsmNY_k2YaE3ofQY12uetea_qtktFznWP7nGOGTre1YtWj_0N_g-A_wL28q</recordid><startdate>20111121</startdate><enddate>20111121</enddate><creator>Kim, Eunjoo</creator><creator>Kim, Hyun-Chul</creator><creator>Lee, Se Geun</creator><creator>Lee, Sung Jun</creator><creator>Go, Tae-Jung</creator><creator>Baek, Chul Su</creator><creator>Jeong, Sang Won</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20111121</creationdate><title>C-Reactive protein-directed immobilization of phosphocholine ligands on a solid surface</title><author>Kim, Eunjoo ; Kim, Hyun-Chul ; Lee, Se Geun ; Lee, Sung Jun ; Go, Tae-Jung ; Baek, Chul Su ; Jeong, Sang Won</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-b11cbfe2de66038573390af7e2d4a5b14c74247d59ec52ea82ca173d3939cdc63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Antibodies, Immobilized - immunology</topic><topic>C-Reactive Protein - analysis</topic><topic>C-Reactive Protein - immunology</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Ligands</topic><topic>Molecular Imprinting</topic><topic>Oils - chemistry</topic><topic>Phosphorylcholine - chemistry</topic><topic>Polystyrenes - chemistry</topic><topic>Surface Properties</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Eunjoo</creatorcontrib><creatorcontrib>Kim, Hyun-Chul</creatorcontrib><creatorcontrib>Lee, Se Geun</creatorcontrib><creatorcontrib>Lee, Sung Jun</creatorcontrib><creatorcontrib>Go, Tae-Jung</creatorcontrib><creatorcontrib>Baek, Chul Su</creatorcontrib><creatorcontrib>Jeong, Sang Won</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical communications (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Eunjoo</au><au>Kim, Hyun-Chul</au><au>Lee, Se Geun</au><au>Lee, Sung Jun</au><au>Go, Tae-Jung</au><au>Baek, Chul Su</au><au>Jeong, Sang Won</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>C-Reactive protein-directed immobilization of phosphocholine ligands on a solid surface</atitle><jtitle>Chemical communications (Cambridge, England)</jtitle><addtitle>Chem Commun (Camb)</addtitle><date>2011-11-21</date><risdate>2011</risdate><volume>47</volume><issue>43</issue><spage>11900</spage><epage>11902</epage><pages>11900-11902</pages><issn>1359-7345</issn><eissn>1364-548X</eissn><abstract>The complexes of C-reactive protein (CRP) with its polymerizable phosphocholine ligands adsorbed at the styrene-water interface were polymerized. 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| ispartof | Chemical communications (Cambridge, England), 2011-11, Vol.47 (43), p.11900-11902 |
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| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Antibodies, Immobilized - immunology C-Reactive Protein - analysis C-Reactive Protein - immunology Humans Immunoassay - methods Ligands Molecular Imprinting Oils - chemistry Phosphorylcholine - chemistry Polystyrenes - chemistry Surface Properties Water - chemistry |
| title | C-Reactive protein-directed immobilization of phosphocholine ligands on a solid surface |
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