Nanopore-Based Single-Molecule Mass Spectrometry on a Lipid Membrane Microarray
We report on parallel high-resolution electrical single-molecule analysis on a chip-based nanopore microarray. Lipid bilayers of
Gespeichert in:
| Veröffentlicht in: | ACS nano 2011-10, Vol.5 (10), p.8080-8088 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 8088 |
|---|---|
| container_issue | 10 |
| container_start_page | 8080 |
| container_title | ACS nano |
| container_volume | 5 |
| creator | Baaken, Gerhard Ankri, Norbert Schuler, Anne-Katrin Rühe, Jürgen Behrends, Jan C |
| description | We report on parallel high-resolution electrical single-molecule analysis on a chip-based nanopore microarray. Lipid bilayers of |
| doi_str_mv | 10.1021/nn202670z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_900641490</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1762057654</sourcerecordid><originalsourceid>FETCH-LOGICAL-a446t-fcd81f595734a19b812c46ee48264e58b7ff4bf169dc0b16aeb46620dff98d9e3</originalsourceid><addsrcrecordid>eNqF0U9PwyAYBnBiNE6nB7-A6cWohypQoOWoi_-SzR2mibeG0hfTpS0V2sP89GI2dzJ64k345Qm8D0InBF8RTMl121JMRYo_d9ABkYmIcSbedrczJyN06P0SY55mqdhHIxpuaJgP0PxZtbazDuJb5aGMFlX7XkM8szXooYZopryPFh3o3tkGereKbBupaFp1VRnNoCmcaoOqtLPKObU6QntG1R6ON-cYvd7fvUwe4-n84WlyM40VY6KPjS4zYrjkacIUkUVGqGYCgGVUMOBZkRrDCkOELDUuiFBQMCEoLo2RWSkhGaPzdW7n7McAvs-bymuo6_AcO_hcYiwYYRIHefGnJGnI5ang7H_KGcMEJ0IGermm4ePeOzB556pGuVVOcP5dSr4tJdjTTexQNFBu5U8LAZytgdI-X9rBtWF1vwR9AW7CklI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1544010369</pqid></control><display><type>article</type><title>Nanopore-Based Single-Molecule Mass Spectrometry on a Lipid Membrane Microarray</title><source>MEDLINE</source><source>ACS Publications</source><creator>Baaken, Gerhard ; Ankri, Norbert ; Schuler, Anne-Katrin ; Rühe, Jürgen ; Behrends, Jan C</creator><creatorcontrib>Baaken, Gerhard ; Ankri, Norbert ; Schuler, Anne-Katrin ; Rühe, Jürgen ; Behrends, Jan C</creatorcontrib><description>We report on parallel high-resolution electrical single-molecule analysis on a chip-based nanopore microarray. Lipid bilayers of <20 μm diameter containing single alpha-hemolysin pores were formed on arrays of subpicoliter cavities containing individual microelectrodes (microelectrode cavity array, MECA), and ion conductance-based single molecule mass spectrometry was performed on mixtures of poly(ethylene glycol) molecules of different length. We thereby demonstrate the function of the MECA device as a chip-based platform for array-format nanopore recordings with a resolution at least equal to that of established single microbilayer supports. We conclude that devices based on MECAs may enable more widespread analytical use of nanopores by providing the high throughput and ease of operation of a high-density array format while maintaining or exceeding the precision of state-of-the-art microbilayer recordings.</description><identifier>ISSN: 1936-0851</identifier><identifier>EISSN: 1936-086X</identifier><identifier>DOI: 10.1021/nn202670z</identifier><identifier>PMID: 21932787</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Arrays ; Cell Membrane - chemistry ; Devices ; Electric Conductivity ; Hemolysin Proteins - chemistry ; Holes ; Lipid Bilayers - chemistry ; Lipids ; Mass spectrometry ; Mass Spectrometry - instrumentation ; Mathematical analysis ; Microarray Analysis - instrumentation ; Microelectrodes ; Nanopores ; Nanostructure ; Polyethylene Glycols - chemistry ; Recording</subject><ispartof>ACS nano, 2011-10, Vol.5 (10), p.8080-8088</ispartof><rights>Copyright © 2011 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a446t-fcd81f595734a19b812c46ee48264e58b7ff4bf169dc0b16aeb46620dff98d9e3</citedby><cites>FETCH-LOGICAL-a446t-fcd81f595734a19b812c46ee48264e58b7ff4bf169dc0b16aeb46620dff98d9e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/nn202670z$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/nn202670z$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2751,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21932787$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baaken, Gerhard</creatorcontrib><creatorcontrib>Ankri, Norbert</creatorcontrib><creatorcontrib>Schuler, Anne-Katrin</creatorcontrib><creatorcontrib>Rühe, Jürgen</creatorcontrib><creatorcontrib>Behrends, Jan C</creatorcontrib><title>Nanopore-Based Single-Molecule Mass Spectrometry on a Lipid Membrane Microarray</title><title>ACS nano</title><addtitle>ACS Nano</addtitle><description>We report on parallel high-resolution electrical single-molecule analysis on a chip-based nanopore microarray. Lipid bilayers of <20 μm diameter containing single alpha-hemolysin pores were formed on arrays of subpicoliter cavities containing individual microelectrodes (microelectrode cavity array, MECA), and ion conductance-based single molecule mass spectrometry was performed on mixtures of poly(ethylene glycol) molecules of different length. We thereby demonstrate the function of the MECA device as a chip-based platform for array-format nanopore recordings with a resolution at least equal to that of established single microbilayer supports. We conclude that devices based on MECAs may enable more widespread analytical use of nanopores by providing the high throughput and ease of operation of a high-density array format while maintaining or exceeding the precision of state-of-the-art microbilayer recordings.</description><subject>Arrays</subject><subject>Cell Membrane - chemistry</subject><subject>Devices</subject><subject>Electric Conductivity</subject><subject>Hemolysin Proteins - chemistry</subject><subject>Holes</subject><subject>Lipid Bilayers - chemistry</subject><subject>Lipids</subject><subject>Mass spectrometry</subject><subject>Mass Spectrometry - instrumentation</subject><subject>Mathematical analysis</subject><subject>Microarray Analysis - instrumentation</subject><subject>Microelectrodes</subject><subject>Nanopores</subject><subject>Nanostructure</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Recording</subject><issn>1936-0851</issn><issn>1936-086X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U9PwyAYBnBiNE6nB7-A6cWohypQoOWoi_-SzR2mibeG0hfTpS0V2sP89GI2dzJ64k345Qm8D0InBF8RTMl121JMRYo_d9ABkYmIcSbedrczJyN06P0SY55mqdhHIxpuaJgP0PxZtbazDuJb5aGMFlX7XkM8szXooYZopryPFh3o3tkGereKbBupaFp1VRnNoCmcaoOqtLPKObU6QntG1R6ON-cYvd7fvUwe4-n84WlyM40VY6KPjS4zYrjkacIUkUVGqGYCgGVUMOBZkRrDCkOELDUuiFBQMCEoLo2RWSkhGaPzdW7n7McAvs-bymuo6_AcO_hcYiwYYRIHefGnJGnI5ang7H_KGcMEJ0IGermm4ePeOzB556pGuVVOcP5dSr4tJdjTTexQNFBu5U8LAZytgdI-X9rBtWF1vwR9AW7CklI</recordid><startdate>20111025</startdate><enddate>20111025</enddate><creator>Baaken, Gerhard</creator><creator>Ankri, Norbert</creator><creator>Schuler, Anne-Katrin</creator><creator>Rühe, Jürgen</creator><creator>Behrends, Jan C</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20111025</creationdate><title>Nanopore-Based Single-Molecule Mass Spectrometry on a Lipid Membrane Microarray</title><author>Baaken, Gerhard ; Ankri, Norbert ; Schuler, Anne-Katrin ; Rühe, Jürgen ; Behrends, Jan C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a446t-fcd81f595734a19b812c46ee48264e58b7ff4bf169dc0b16aeb46620dff98d9e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Arrays</topic><topic>Cell Membrane - chemistry</topic><topic>Devices</topic><topic>Electric Conductivity</topic><topic>Hemolysin Proteins - chemistry</topic><topic>Holes</topic><topic>Lipid Bilayers - chemistry</topic><topic>Lipids</topic><topic>Mass spectrometry</topic><topic>Mass Spectrometry - instrumentation</topic><topic>Mathematical analysis</topic><topic>Microarray Analysis - instrumentation</topic><topic>Microelectrodes</topic><topic>Nanopores</topic><topic>Nanostructure</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Recording</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baaken, Gerhard</creatorcontrib><creatorcontrib>Ankri, Norbert</creatorcontrib><creatorcontrib>Schuler, Anne-Katrin</creatorcontrib><creatorcontrib>Rühe, Jürgen</creatorcontrib><creatorcontrib>Behrends, Jan C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>ACS nano</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baaken, Gerhard</au><au>Ankri, Norbert</au><au>Schuler, Anne-Katrin</au><au>Rühe, Jürgen</au><au>Behrends, Jan C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanopore-Based Single-Molecule Mass Spectrometry on a Lipid Membrane Microarray</atitle><jtitle>ACS nano</jtitle><addtitle>ACS Nano</addtitle><date>2011-10-25</date><risdate>2011</risdate><volume>5</volume><issue>10</issue><spage>8080</spage><epage>8088</epage><pages>8080-8088</pages><issn>1936-0851</issn><eissn>1936-086X</eissn><abstract>We report on parallel high-resolution electrical single-molecule analysis on a chip-based nanopore microarray. Lipid bilayers of <20 μm diameter containing single alpha-hemolysin pores were formed on arrays of subpicoliter cavities containing individual microelectrodes (microelectrode cavity array, MECA), and ion conductance-based single molecule mass spectrometry was performed on mixtures of poly(ethylene glycol) molecules of different length. We thereby demonstrate the function of the MECA device as a chip-based platform for array-format nanopore recordings with a resolution at least equal to that of established single microbilayer supports. We conclude that devices based on MECAs may enable more widespread analytical use of nanopores by providing the high throughput and ease of operation of a high-density array format while maintaining or exceeding the precision of state-of-the-art microbilayer recordings.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>21932787</pmid><doi>10.1021/nn202670z</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1936-0851 |
| ispartof | ACS nano, 2011-10, Vol.5 (10), p.8080-8088 |
| issn | 1936-0851 1936-086X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_900641490 |
| source | MEDLINE; ACS Publications |
| subjects | Arrays Cell Membrane - chemistry Devices Electric Conductivity Hemolysin Proteins - chemistry Holes Lipid Bilayers - chemistry Lipids Mass spectrometry Mass Spectrometry - instrumentation Mathematical analysis Microarray Analysis - instrumentation Microelectrodes Nanopores Nanostructure Polyethylene Glycols - chemistry Recording |
| title | Nanopore-Based Single-Molecule Mass Spectrometry on a Lipid Membrane Microarray |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T14%3A46%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nanopore-Based%20Single-Molecule%20Mass%20Spectrometry%20on%20a%20Lipid%20Membrane%20Microarray&rft.jtitle=ACS%20nano&rft.au=Baaken,%20Gerhard&rft.date=2011-10-25&rft.volume=5&rft.issue=10&rft.spage=8080&rft.epage=8088&rft.pages=8080-8088&rft.issn=1936-0851&rft.eissn=1936-086X&rft_id=info:doi/10.1021/nn202670z&rft_dat=%3Cproquest_cross%3E1762057654%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1544010369&rft_id=info:pmid/21932787&rfr_iscdi=true |