Characterization of Novel RFamide Peptides in the Central Nervous System of the Brown Hagfish: Isolation, Localization, and Functional Analysis
RFamide (RFa) peptides play various important roles in the central nervous system in both invertebrates and vertebrates. However, there is no evidence of the existence of any RFamide peptide in the brain of hagfish, one of the oldest lineages of vertebrates. In this study, we sought to identify nove...
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Veröffentlicht in: | Endocrinology (Philadelphia) 2011-11, Vol.152 (11), p.4252-4264 |
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description | RFamide (RFa) peptides play various important roles in the central nervous system in both invertebrates and vertebrates. However, there is no evidence of the existence of any RFamide peptide in the brain of hagfish, one of the oldest lineages of vertebrates. In this study, we sought to identify novel RFamide peptides from the brains of hagfish (Paramyxine atami). We identified four novel RFamide peptides, which had the C-terminal Pro-Gln-Arg-Phe-NH2 structure. cDNA cloning revealed that the identified RFamide peptides are encoded in two types of cDNA. Molecular phylogenetic analysis of the two precursors indicated that the hagfish RFamide peptides belong to the PQRFamide peptide group that includes mammalian neuropeptide FF and AF. Based on immunohistochemistry and in situ hybridization, hagfish PQRFamide peptide precursor mRNA and its translated peptides were localized in the infundibular nucleus of the hypothalamus. Immunoreactive fibers were terminated on blood vessels in the infundibular nucleus. Dense immunoreactive fibers were also observed in other brain regions. We further showed that one of the hagfish PQRFamide peptides significantly stimulated the expression of gonadotropin-β mRNA in the cultured hagfish pituitary. These results indicate that the control mechanism of gonadotropin expression by a hypothalamic neuropeptide evolved in the agnathan brain. This is the first evidence describing the identification of RFamide peptides in the hagfish brain. This is also the first report showing the regulation of gonadotropin expression by a homolog of neuropeptide FF that belongs to the PQRFamide peptide group in any vertebrate. |
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However, there is no evidence of the existence of any RFamide peptide in the brain of hagfish, one of the oldest lineages of vertebrates. In this study, we sought to identify novel RFamide peptides from the brains of hagfish (Paramyxine atami). We identified four novel RFamide peptides, which had the C-terminal Pro-Gln-Arg-Phe-NH2 structure. cDNA cloning revealed that the identified RFamide peptides are encoded in two types of cDNA. Molecular phylogenetic analysis of the two precursors indicated that the hagfish RFamide peptides belong to the PQRFamide peptide group that includes mammalian neuropeptide FF and AF. Based on immunohistochemistry and in situ hybridization, hagfish PQRFamide peptide precursor mRNA and its translated peptides were localized in the infundibular nucleus of the hypothalamus. Immunoreactive fibers were terminated on blood vessels in the infundibular nucleus. Dense immunoreactive fibers were also observed in other brain regions. We further showed that one of the hagfish PQRFamide peptides significantly stimulated the expression of gonadotropin-β mRNA in the cultured hagfish pituitary. These results indicate that the control mechanism of gonadotropin expression by a hypothalamic neuropeptide evolved in the agnathan brain. This is the first evidence describing the identification of RFamide peptides in the hagfish brain. This is also the first report showing the regulation of gonadotropin expression by a homolog of neuropeptide FF that belongs to the PQRFamide peptide group in any vertebrate.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/en.2011-1375</identifier><identifier>PMID: 21862614</identifier><identifier>CODEN: ENDOAO</identifier><language>eng</language><publisher>Chevy Chase, MD: Endocrine Society</publisher><subject>Animals ; Biological and medical sciences ; Blood vessels ; Brain ; Brain - metabolism ; Central nervous system ; Cloning ; Female ; Fibers ; Functional analysis ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gonadotropins ; Hagfishes - genetics ; Hagfishes - metabolism ; Hemopoiesis ; Hybridization ; Hypothalamus ; Immunohistochemistry ; Localization ; Male ; Molecular Sequence Data ; Molecular structure ; mRNA ; Nervous system ; Neuropeptides ; Neuropeptides - analysis ; Neuropeptides - genetics ; Neuropeptides - metabolism ; Peptides ; Phylogeny ; Pituitary ; Pituitary (anterior) ; Pituitary Gland - metabolism ; Precursors ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Vertebrates ; Vertebrates: endocrinology</subject><ispartof>Endocrinology (Philadelphia), 2011-11, Vol.152 (11), p.4252-4264</ispartof><rights>Copyright © 2011 by The Endocrine Society</rights><rights>Copyright © 2011 by The Endocrine Society 2011</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-54cc1ed994cf7948388950196ecc740b00215a111df5aa5c71baebfc67be69203</citedby><cites>FETCH-LOGICAL-c528t-54cc1ed994cf7948388950196ecc740b00215a111df5aa5c71baebfc67be69203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24712371$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21862614$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Osugi, Tomohiro</creatorcontrib><creatorcontrib>Uchida, Katsuhisa</creatorcontrib><creatorcontrib>Nozaki, Masumi</creatorcontrib><creatorcontrib>Tsutsui, Kazuyoshi</creatorcontrib><title>Characterization of Novel RFamide Peptides in the Central Nervous System of the Brown Hagfish: Isolation, Localization, and Functional Analysis</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description>RFamide (RFa) peptides play various important roles in the central nervous system in both invertebrates and vertebrates. However, there is no evidence of the existence of any RFamide peptide in the brain of hagfish, one of the oldest lineages of vertebrates. In this study, we sought to identify novel RFamide peptides from the brains of hagfish (Paramyxine atami). We identified four novel RFamide peptides, which had the C-terminal Pro-Gln-Arg-Phe-NH2 structure. cDNA cloning revealed that the identified RFamide peptides are encoded in two types of cDNA. Molecular phylogenetic analysis of the two precursors indicated that the hagfish RFamide peptides belong to the PQRFamide peptide group that includes mammalian neuropeptide FF and AF. Based on immunohistochemistry and in situ hybridization, hagfish PQRFamide peptide precursor mRNA and its translated peptides were localized in the infundibular nucleus of the hypothalamus. Immunoreactive fibers were terminated on blood vessels in the infundibular nucleus. Dense immunoreactive fibers were also observed in other brain regions. We further showed that one of the hagfish PQRFamide peptides significantly stimulated the expression of gonadotropin-β mRNA in the cultured hagfish pituitary. These results indicate that the control mechanism of gonadotropin expression by a hypothalamic neuropeptide evolved in the agnathan brain. This is the first evidence describing the identification of RFamide peptides in the hagfish brain. This is also the first report showing the regulation of gonadotropin expression by a homolog of neuropeptide FF that belongs to the PQRFamide peptide group in any vertebrate.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood vessels</subject><subject>Brain</subject><subject>Brain - metabolism</subject><subject>Central nervous system</subject><subject>Cloning</subject><subject>Female</subject><subject>Fibers</subject><subject>Functional analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gonadotropins</subject><subject>Hagfishes - genetics</subject><subject>Hagfishes - metabolism</subject><subject>Hemopoiesis</subject><subject>Hybridization</subject><subject>Hypothalamus</subject><subject>Immunohistochemistry</subject><subject>Localization</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Molecular structure</subject><subject>mRNA</subject><subject>Nervous system</subject><subject>Neuropeptides</subject><subject>Neuropeptides - analysis</subject><subject>Neuropeptides - genetics</subject><subject>Neuropeptides - metabolism</subject><subject>Peptides</subject><subject>Phylogeny</subject><subject>Pituitary</subject><subject>Pituitary (anterior)</subject><subject>Pituitary Gland - metabolism</subject><subject>Precursors</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Vertebrates</subject><subject>Vertebrates: endocrinology</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU9v1DAQxS0EotvCjTOyhFAvm-JxnDjhVlYsrbQqiD_nyHEmrKusndpJ0fIl-Mo4bGAlBBePRv75vRk_Qp4BuwAO7BXaC84AEkhl9oAsoBRZIkGyh2TBGKSJ5FyekNMQbmMrhEgfkxMORc5zEAvyY7VVXukBvfmuBuMsdS29cffY0Y9rtTMN0g_YD7EGaiwdtkhXaAevOnqD_t6NgX7ahwF307vp9o133yy9Ul9bE7av6XVw3S_dJd04rbrZZUmVbeh6tHrqothlPPbBhCfkUau6gE_neka-rN9-Xl0lm_fvrleXm0RnvBiSTGgN2JSl0K0sRZEWRZkxKHPUWgpWM8YhUwDQtJlSmZZQK6xbncsa85Kz9IycH3R77-5GDEO1M0Fj1ymLcamqZCxPBRd5JF_8Rd660cdxQ5VCynJWiKKI1PJAae9C8NhWvTc75fcVsGrKqUJbTTlVU04Rfz6LjvUOmz_w72Ai8HIGVIjf1npltQlHTkjgqYTjHm7s_2eZzJbpgUTbOO2Nxd5jCMdt_jnoT8g2uAk</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>Osugi, Tomohiro</creator><creator>Uchida, Katsuhisa</creator><creator>Nozaki, Masumi</creator><creator>Tsutsui, Kazuyoshi</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20111101</creationdate><title>Characterization of Novel RFamide Peptides in the Central Nervous System of the Brown Hagfish: Isolation, Localization, and Functional Analysis</title><author>Osugi, Tomohiro ; Uchida, Katsuhisa ; Nozaki, Masumi ; Tsutsui, Kazuyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-54cc1ed994cf7948388950196ecc740b00215a111df5aa5c71baebfc67be69203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood vessels</topic><topic>Brain</topic><topic>Brain - metabolism</topic><topic>Central nervous system</topic><topic>Cloning</topic><topic>Female</topic><topic>Fibers</topic><topic>Functional analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gonadotropins</topic><topic>Hagfishes - genetics</topic><topic>Hagfishes - metabolism</topic><topic>Hemopoiesis</topic><topic>Hybridization</topic><topic>Hypothalamus</topic><topic>Immunohistochemistry</topic><topic>Localization</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>Molecular structure</topic><topic>mRNA</topic><topic>Nervous system</topic><topic>Neuropeptides</topic><topic>Neuropeptides - analysis</topic><topic>Neuropeptides - genetics</topic><topic>Neuropeptides - metabolism</topic><topic>Peptides</topic><topic>Phylogeny</topic><topic>Pituitary</topic><topic>Pituitary (anterior)</topic><topic>Pituitary Gland - metabolism</topic><topic>Precursors</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Vertebrates</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Osugi, Tomohiro</creatorcontrib><creatorcontrib>Uchida, Katsuhisa</creatorcontrib><creatorcontrib>Nozaki, Masumi</creatorcontrib><creatorcontrib>Tsutsui, Kazuyoshi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Osugi, Tomohiro</au><au>Uchida, Katsuhisa</au><au>Nozaki, Masumi</au><au>Tsutsui, Kazuyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Novel RFamide Peptides in the Central Nervous System of the Brown Hagfish: Isolation, Localization, and Functional Analysis</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>2011-11-01</date><risdate>2011</risdate><volume>152</volume><issue>11</issue><spage>4252</spage><epage>4264</epage><pages>4252-4264</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><coden>ENDOAO</coden><abstract>RFamide (RFa) peptides play various important roles in the central nervous system in both invertebrates and vertebrates. However, there is no evidence of the existence of any RFamide peptide in the brain of hagfish, one of the oldest lineages of vertebrates. In this study, we sought to identify novel RFamide peptides from the brains of hagfish (Paramyxine atami). We identified four novel RFamide peptides, which had the C-terminal Pro-Gln-Arg-Phe-NH2 structure. cDNA cloning revealed that the identified RFamide peptides are encoded in two types of cDNA. Molecular phylogenetic analysis of the two precursors indicated that the hagfish RFamide peptides belong to the PQRFamide peptide group that includes mammalian neuropeptide FF and AF. Based on immunohistochemistry and in situ hybridization, hagfish PQRFamide peptide precursor mRNA and its translated peptides were localized in the infundibular nucleus of the hypothalamus. Immunoreactive fibers were terminated on blood vessels in the infundibular nucleus. Dense immunoreactive fibers were also observed in other brain regions. We further showed that one of the hagfish PQRFamide peptides significantly stimulated the expression of gonadotropin-β mRNA in the cultured hagfish pituitary. These results indicate that the control mechanism of gonadotropin expression by a hypothalamic neuropeptide evolved in the agnathan brain. This is the first evidence describing the identification of RFamide peptides in the hagfish brain. This is also the first report showing the regulation of gonadotropin expression by a homolog of neuropeptide FF that belongs to the PQRFamide peptide group in any vertebrate.</abstract><cop>Chevy Chase, MD</cop><pub>Endocrine Society</pub><pmid>21862614</pmid><doi>10.1210/en.2011-1375</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Biological and medical sciences Blood vessels Brain Brain - metabolism Central nervous system Cloning Female Fibers Functional analysis Fundamental and applied biological sciences. Psychology Gene expression Gonadotropins Hagfishes - genetics Hagfishes - metabolism Hemopoiesis Hybridization Hypothalamus Immunohistochemistry Localization Male Molecular Sequence Data Molecular structure mRNA Nervous system Neuropeptides Neuropeptides - analysis Neuropeptides - genetics Neuropeptides - metabolism Peptides Phylogeny Pituitary Pituitary (anterior) Pituitary Gland - metabolism Precursors RNA, Messenger - genetics RNA, Messenger - metabolism Vertebrates Vertebrates: endocrinology |
title | Characterization of Novel RFamide Peptides in the Central Nervous System of the Brown Hagfish: Isolation, Localization, and Functional Analysis |
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