Protective properties of quercetin against DNA damage and oxidative stress induced by methylmercury in rats
Aim of the study was to find out whether consumption of quercetin (QC), an abundant flavonoid in the human diet, protects against DNA damage caused by exposure to organic mercury. Therefore, rats were treated orally with methylmercury (MeHg) and the flavonoid with doses that reflect the human exposu...
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| Veröffentlicht in: | Archives of toxicology 2011-09, Vol.85 (9), p.1151-1157 |
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| creator | Barcelos, Gustavo Rafael Mazzaron Grotto, Denise Serpeloni, Juliana Mara Angeli, José Pedro Friedmann Rocha, Bruno Alves de Oliveira Souza, Vanessa Cristina Vicentini, Juliana Tanara Emanuelli, Tatiana Bastos, Jairo Kenupp Antunes, Lusânia Maria Greggi Knasmüller, Siegfried Barbosa, Fernando |
| description | Aim of the study was to find out whether consumption of quercetin (QC), an abundant flavonoid in the human diet, protects against DNA damage caused by exposure to organic mercury. Therefore, rats were treated orally with methylmercury (MeHg) and the flavonoid with doses that reflect the human exposure. The animals received MeHg (30 μg/kg/bw/day), QC (0.5–50 mg/kg/bw/day), or combinations of both over 45 days. Subsequently, the glutathione levels (GSH) and the activities of glutathione peroxidase (GPx) and catalase (CAT) were determined, and DNA damage was measured in hepatocytes and peripheral leukocytes in single cell gel electrophoresis assays. MeHg decreased the concentration of GSH and the activity of GPx by 17 and 12%, respectively and caused DNA damage to liver and blood cells, while with QC no such effects were seen. When the flavonoid was given in combination with MeHg, the intermediate and the highest concentrations (5.0 and 50.0 mg/kg/bw/day) were found to cause DNA protection; DNA migration was reduced by 54 and 65% in the hepatocytes and by 27 and 36% in the leukocytes; furthermore, the reduction in GSH and GPx levels caused by MeHg treatment was restored. In summary, our results indicate that consumption of QC-rich foods may protect Hg-exposed humans against the adverse health effects of the metal. |
| doi_str_mv | 10.1007/s00204-011-0652-y |
| format | Article |
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Therefore, rats were treated orally with methylmercury (MeHg) and the flavonoid with doses that reflect the human exposure. The animals received MeHg (30 μg/kg/bw/day), QC (0.5–50 mg/kg/bw/day), or combinations of both over 45 days. Subsequently, the glutathione levels (GSH) and the activities of glutathione peroxidase (GPx) and catalase (CAT) were determined, and DNA damage was measured in hepatocytes and peripheral leukocytes in single cell gel electrophoresis assays. MeHg decreased the concentration of GSH and the activity of GPx by 17 and 12%, respectively and caused DNA damage to liver and blood cells, while with QC no such effects were seen. When the flavonoid was given in combination with MeHg, the intermediate and the highest concentrations (5.0 and 50.0 mg/kg/bw/day) were found to cause DNA protection; DNA migration was reduced by 54 and 65% in the hepatocytes and by 27 and 36% in the leukocytes; furthermore, the reduction in GSH and GPx levels caused by MeHg treatment was restored. In summary, our results indicate that consumption of QC-rich foods may protect Hg-exposed humans against the adverse health effects of the metal.</description><identifier>ISSN: 0340-5761</identifier><identifier>EISSN: 1432-0738</identifier><identifier>DOI: 10.1007/s00204-011-0652-y</identifier><identifier>PMID: 21286687</identifier><identifier>CODEN: ARTODN</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animals ; Antioxidants ; Antioxidants - pharmacology ; Bioassays ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Catalase - metabolism ; Chemical and industrial products toxicology. Toxic occupational diseases ; Comet Assay ; DNA damage ; DNA Damage - drug effects ; Environmental Health ; Genotoxicity and Carcinogenicity ; Glutathione - metabolism ; Glutathione Peroxidase - metabolism ; Hepatocytes - drug effects ; Hepatocytes - enzymology ; Hepatocytes - metabolism ; Leukocytes - drug effects ; Leukocytes - enzymology ; Leukocytes - metabolism ; Liver - drug effects ; Liver - enzymology ; Liver - metabolism ; Male ; Medical sciences ; Mercury ; Metals and various inorganic compounds ; Methylmercury Compounds - blood ; Methylmercury Compounds - pharmacokinetics ; Methylmercury Compounds - toxicity ; Mutagens - pharmacokinetics ; Mutagens - toxicity ; Occupational Medicine/Industrial Medicine ; Oxidative stress ; Oxidative Stress - drug effects ; Pharmacology/Toxicology ; Quercetin - pharmacology ; Rats ; Rats, Wistar ; Rodents ; Toxicology</subject><ispartof>Archives of toxicology, 2011-09, Vol.85 (9), p.1151-1157</ispartof><rights>Springer-Verlag 2011</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-f6a6088414b9a480c7a7ace10535f3420847bc12c7208c922fdf59f633d977b33</citedby><cites>FETCH-LOGICAL-c498t-f6a6088414b9a480c7a7ace10535f3420847bc12c7208c922fdf59f633d977b33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00204-011-0652-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00204-011-0652-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24462258$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21286687$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barcelos, Gustavo Rafael Mazzaron</creatorcontrib><creatorcontrib>Grotto, Denise</creatorcontrib><creatorcontrib>Serpeloni, Juliana Mara</creatorcontrib><creatorcontrib>Angeli, José Pedro Friedmann</creatorcontrib><creatorcontrib>Rocha, Bruno Alves</creatorcontrib><creatorcontrib>de Oliveira Souza, Vanessa Cristina</creatorcontrib><creatorcontrib>Vicentini, Juliana Tanara</creatorcontrib><creatorcontrib>Emanuelli, Tatiana</creatorcontrib><creatorcontrib>Bastos, Jairo Kenupp</creatorcontrib><creatorcontrib>Antunes, Lusânia Maria Greggi</creatorcontrib><creatorcontrib>Knasmüller, Siegfried</creatorcontrib><creatorcontrib>Barbosa, Fernando</creatorcontrib><title>Protective properties of quercetin against DNA damage and oxidative stress induced by methylmercury in rats</title><title>Archives of toxicology</title><addtitle>Arch Toxicol</addtitle><addtitle>Arch Toxicol</addtitle><description>Aim of the study was to find out whether consumption of quercetin (QC), an abundant flavonoid in the human diet, protects against DNA damage caused by exposure to organic mercury. 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When the flavonoid was given in combination with MeHg, the intermediate and the highest concentrations (5.0 and 50.0 mg/kg/bw/day) were found to cause DNA protection; DNA migration was reduced by 54 and 65% in the hepatocytes and by 27 and 36% in the leukocytes; furthermore, the reduction in GSH and GPx levels caused by MeHg treatment was restored. In summary, our results indicate that consumption of QC-rich foods may protect Hg-exposed humans against the adverse health effects of the metal.</description><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Bioassays</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Catalase - metabolism</subject><subject>Chemical and industrial products toxicology. 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Therefore, rats were treated orally with methylmercury (MeHg) and the flavonoid with doses that reflect the human exposure. The animals received MeHg (30 μg/kg/bw/day), QC (0.5–50 mg/kg/bw/day), or combinations of both over 45 days. Subsequently, the glutathione levels (GSH) and the activities of glutathione peroxidase (GPx) and catalase (CAT) were determined, and DNA damage was measured in hepatocytes and peripheral leukocytes in single cell gel electrophoresis assays. MeHg decreased the concentration of GSH and the activity of GPx by 17 and 12%, respectively and caused DNA damage to liver and blood cells, while with QC no such effects were seen. When the flavonoid was given in combination with MeHg, the intermediate and the highest concentrations (5.0 and 50.0 mg/kg/bw/day) were found to cause DNA protection; DNA migration was reduced by 54 and 65% in the hepatocytes and by 27 and 36% in the leukocytes; furthermore, the reduction in GSH and GPx levels caused by MeHg treatment was restored. In summary, our results indicate that consumption of QC-rich foods may protect Hg-exposed humans against the adverse health effects of the metal.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21286687</pmid><doi>10.1007/s00204-011-0652-y</doi><tpages>7</tpages></addata></record> |
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| ispartof | Archives of toxicology, 2011-09, Vol.85 (9), p.1151-1157 |
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| subjects | Animals Antioxidants Antioxidants - pharmacology Bioassays Biological and medical sciences Biomedical and Life Sciences Biomedicine Catalase - metabolism Chemical and industrial products toxicology. Toxic occupational diseases Comet Assay DNA damage DNA Damage - drug effects Environmental Health Genotoxicity and Carcinogenicity Glutathione - metabolism Glutathione Peroxidase - metabolism Hepatocytes - drug effects Hepatocytes - enzymology Hepatocytes - metabolism Leukocytes - drug effects Leukocytes - enzymology Leukocytes - metabolism Liver - drug effects Liver - enzymology Liver - metabolism Male Medical sciences Mercury Metals and various inorganic compounds Methylmercury Compounds - blood Methylmercury Compounds - pharmacokinetics Methylmercury Compounds - toxicity Mutagens - pharmacokinetics Mutagens - toxicity Occupational Medicine/Industrial Medicine Oxidative stress Oxidative Stress - drug effects Pharmacology/Toxicology Quercetin - pharmacology Rats Rats, Wistar Rodents Toxicology |
| title | Protective properties of quercetin against DNA damage and oxidative stress induced by methylmercury in rats |
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