Production of a heterologous proteinase A by Saccharomyces kluyveri
In order to evaluate the potential of Saccharomyces kluyveri for heterologous protein production, S. kluyveri Y159 was transformed with a S. cerevisiae-based multi-copy plasmid containing the S. cerevisiae PEP4 gene, which encodes proteinase A, under the control of its native promoter. As a referenc...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2001-10, Vol.57 (1/2), p.216-219 |
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| creator | Moller, K Tidemand, L.D Winther, J.R Olsson, L Piskur, J Nielsen, J |
| description | In order to evaluate the potential of Saccharomyces kluyveri for heterologous protein production, S. kluyveri Y159 was transformed with a S. cerevisiae-based multi-copy plasmid containing the S. cerevisiae PEP4 gene, which encodes proteinase A, under the control of its native promoter. As a reference, S. cerevisiae CEN.PK 113-5D was transformed with the same plasmid and the two strains were characterised in batch cultivations on glucose. The glucose metabolism was found to be less fermentative in S. kluyveri than in S. cerevisiae. The yield of ethanol on glucose was 0.11 g/g in S. kluyveri, compared to a yield of 0.40 g/g in S. cerevisiae. Overexpression of PEP4 led to the secretion of active proteinase A in both S. kluyveri and S. cerevisiae. The yield of active proteinase A during growth on glucose was found to be 3.6-fold higher in S. kluyveri than in the S. cerevisiae reference strain. |
| doi_str_mv | 10.1007/s002530100680 |
| format | Article |
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As a reference, S. cerevisiae CEN.PK 113-5D was transformed with the same plasmid and the two strains were characterised in batch cultivations on glucose. The glucose metabolism was found to be less fermentative in S. kluyveri than in S. cerevisiae. The yield of ethanol on glucose was 0.11 g/g in S. kluyveri, compared to a yield of 0.40 g/g in S. cerevisiae. Overexpression of PEP4 led to the secretion of active proteinase A in both S. kluyveri and S. cerevisiae. The yield of active proteinase A during growth on glucose was found to be 3.6-fold higher in S. kluyveri than in the S. cerevisiae reference strain.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s002530100680</identifier><identifier>PMID: 11693924</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Aspartic Acid Endopeptidases - biosynthesis ; Biological and medical sciences ; Biotechnology ; Enzyme engineering ; Ethanol ; Ethanol - metabolism ; Fermentation ; Fundamental and applied biological sciences. Psychology ; genes ; Genetics ; Glucose ; Glucose - metabolism ; heterologous gene expression ; metabolism ; Methods. Procedures. Technologies ; PEP4 gene ; Plasmids ; Production of selected enzymes ; Promoter Regions, Genetic ; proteinases ; Saccharomyces ; Saccharomyces - genetics ; Saccharomyces - metabolism ; Saccharomyces kluyveri ; secretion ; Yeast</subject><ispartof>Applied microbiology and biotechnology, 2001-10, Vol.57 (1/2), p.216-219</ispartof><rights>2002 INIST-CNRS</rights><rights>Springer-Verlag 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-41a52c91204c4c5ab1583c7709240fc2a841fbb5056249b53d39e38231b8b4823</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13400183$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11693924$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moller, K</creatorcontrib><creatorcontrib>Tidemand, L.D</creatorcontrib><creatorcontrib>Winther, J.R</creatorcontrib><creatorcontrib>Olsson, L</creatorcontrib><creatorcontrib>Piskur, J</creatorcontrib><creatorcontrib>Nielsen, J</creatorcontrib><title>Production of a heterologous proteinase A by Saccharomyces kluyveri</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><description>In order to evaluate the potential of Saccharomyces kluyveri for heterologous protein production, S. kluyveri Y159 was transformed with a S. cerevisiae-based multi-copy plasmid containing the S. cerevisiae PEP4 gene, which encodes proteinase A, under the control of its native promoter. As a reference, S. cerevisiae CEN.PK 113-5D was transformed with the same plasmid and the two strains were characterised in batch cultivations on glucose. The glucose metabolism was found to be less fermentative in S. kluyveri than in S. cerevisiae. The yield of ethanol on glucose was 0.11 g/g in S. kluyveri, compared to a yield of 0.40 g/g in S. cerevisiae. Overexpression of PEP4 led to the secretion of active proteinase A in both S. kluyveri and S. cerevisiae. The yield of active proteinase A during growth on glucose was found to be 3.6-fold higher in S. kluyveri than in the S. cerevisiae reference strain.</description><subject>Aspartic Acid Endopeptidases - biosynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Enzyme engineering</subject><subject>Ethanol</subject><subject>Ethanol - metabolism</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genetics</subject><subject>Glucose</subject><subject>Glucose - metabolism</subject><subject>heterologous gene expression</subject><subject>metabolism</subject><subject>Methods. Procedures. Technologies</subject><subject>PEP4 gene</subject><subject>Plasmids</subject><subject>Production of selected enzymes</subject><subject>Promoter Regions, Genetic</subject><subject>proteinases</subject><subject>Saccharomyces</subject><subject>Saccharomyces - genetics</subject><subject>Saccharomyces - metabolism</subject><subject>Saccharomyces kluyveri</subject><subject>secretion</subject><subject>Yeast</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp90c1r2zAYBnAxWtY023HXzhS2nry9r751DGFdC4EWsp6NrMitO8dKJbuQ_77aEijtIadXhx8Pj3gI-YLwAwHUzwRABYP8lho-kAlyRkuQyI_IBFCJUgmjT8hpSo8ASLWUH8kJojTMUD4h89sYVqMb2tAXoSls8eAHH0MX7sOYik0Mg297m3wxK-ptsbTOPdgY1lvnU_G3G7fPPrafyHFju-Q_7--U3F3--jO_Khc3v6_ns0XpuIKh5GgFdQYpcMedsDUKzZxSkItA46jVHJu6FiAk5aYWbMWMZ5oyrHXN852Si11urvU0-jRU6zY533W297ltpY1ByWROnZLvB6WilHPxP_IwRM0U3yWev4OPYYx9_m6ldRaMa8io3CEXQ0rRN9UmtmsbtxVC9W-t6s1a2Z_tQ8d67Vevej9PBt_2wCZnuyba3rXp1TGeN9Usu68719hQ2fuYzd2SAkoAMIpxw14ArpiiIA</recordid><startdate>20011001</startdate><enddate>20011001</enddate><creator>Moller, K</creator><creator>Tidemand, L.D</creator><creator>Winther, J.R</creator><creator>Olsson, L</creator><creator>Piskur, J</creator><creator>Nielsen, J</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20011001</creationdate><title>Production of a heterologous proteinase A by Saccharomyces kluyveri</title><author>Moller, K ; Tidemand, L.D ; Winther, J.R ; Olsson, L ; Piskur, J ; Nielsen, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-41a52c91204c4c5ab1583c7709240fc2a841fbb5056249b53d39e38231b8b4823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Aspartic Acid Endopeptidases - biosynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Enzyme engineering</topic><topic>Ethanol</topic><topic>Ethanol - metabolism</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Genetics</topic><topic>Glucose</topic><topic>Glucose - metabolism</topic><topic>heterologous gene expression</topic><topic>metabolism</topic><topic>Methods. Procedures. Technologies</topic><topic>PEP4 gene</topic><topic>Plasmids</topic><topic>Production of selected enzymes</topic><topic>Promoter Regions, Genetic</topic><topic>proteinases</topic><topic>Saccharomyces</topic><topic>Saccharomyces - genetics</topic><topic>Saccharomyces - metabolism</topic><topic>Saccharomyces kluyveri</topic><topic>secretion</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moller, K</creatorcontrib><creatorcontrib>Tidemand, L.D</creatorcontrib><creatorcontrib>Winther, J.R</creatorcontrib><creatorcontrib>Olsson, L</creatorcontrib><creatorcontrib>Piskur, J</creatorcontrib><creatorcontrib>Nielsen, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>One Business (ProQuest)</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moller, K</au><au>Tidemand, L.D</au><au>Winther, J.R</au><au>Olsson, L</au><au>Piskur, J</au><au>Nielsen, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of a heterologous proteinase A by Saccharomyces kluyveri</atitle><jtitle>Applied microbiology and biotechnology</jtitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2001-10-01</date><risdate>2001</risdate><volume>57</volume><issue>1/2</issue><spage>216</spage><epage>219</epage><pages>216-219</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>In order to evaluate the potential of Saccharomyces kluyveri for heterologous protein production, S. kluyveri Y159 was transformed with a S. cerevisiae-based multi-copy plasmid containing the S. cerevisiae PEP4 gene, which encodes proteinase A, under the control of its native promoter. As a reference, S. cerevisiae CEN.PK 113-5D was transformed with the same plasmid and the two strains were characterised in batch cultivations on glucose. The glucose metabolism was found to be less fermentative in S. kluyveri than in S. cerevisiae. The yield of ethanol on glucose was 0.11 g/g in S. kluyveri, compared to a yield of 0.40 g/g in S. cerevisiae. Overexpression of PEP4 led to the secretion of active proteinase A in both S. kluyveri and S. cerevisiae. The yield of active proteinase A during growth on glucose was found to be 3.6-fold higher in S. kluyveri than in the S. cerevisiae reference strain.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>11693924</pmid><doi>10.1007/s002530100680</doi><tpages>4</tpages></addata></record> |
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| ispartof | Applied microbiology and biotechnology, 2001-10, Vol.57 (1/2), p.216-219 |
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| subjects | Aspartic Acid Endopeptidases - biosynthesis Biological and medical sciences Biotechnology Enzyme engineering Ethanol Ethanol - metabolism Fermentation Fundamental and applied biological sciences. Psychology genes Genetics Glucose Glucose - metabolism heterologous gene expression metabolism Methods. Procedures. Technologies PEP4 gene Plasmids Production of selected enzymes Promoter Regions, Genetic proteinases Saccharomyces Saccharomyces - genetics Saccharomyces - metabolism Saccharomyces kluyveri secretion Yeast |
| title | Production of a heterologous proteinase A by Saccharomyces kluyveri |
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