in vivo genotoxicity of cisplatin, isoflurane and halothane evaluated by alkaline comet assay in Swiss albino mice
The aim of this study was to evaluate the genotoxicity of repeated exposure to isoflurane or halothane and compare it with the genotoxicity of repeated exposure to cisplatin. We also determined the genotoxicity of combined treatment with inhalation anaesthetics and cisplatin on peripheral blood leuc...
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| Veröffentlicht in: | Journal of applied genetics 2011-08, Vol.52 (3), p.355-361 |
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| creator | Brozovic, Gordana Orsolic, Nada Knezevic, Fabijan Horvat Knezevic, Anica Benkovic, Vesna Sakic, Katarina Borojevic, Nikola Dikic, Domagoj |
| description | The aim of this study was to evaluate the genotoxicity of repeated exposure to isoflurane or halothane and compare it with the genotoxicity of repeated exposure to cisplatin. We also determined the genotoxicity of combined treatment with inhalation anaesthetics and cisplatin on peripheral blood leucocytes (PBL), brain, liver and kidney cells of mice. The mice were divided into six groups as follows: control, cisplatin, isoflurane, cisplatin–isoflurane, halothane and cisplatin–halothane, and were exposed respectively for three consecutive days. The mice were treated with cisplatin or exposed to inhalation anaesthetic; the combined groups were exposed to inhalation anaesthetic after treatment with cisplatin. The alkaline comet assay was performed. All drugs had a strong genotoxicity (P |
| doi_str_mv | 10.1007/s13353-011-0046-0 |
| format | Article |
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We also determined the genotoxicity of combined treatment with inhalation anaesthetics and cisplatin on peripheral blood leucocytes (PBL), brain, liver and kidney cells of mice. The mice were divided into six groups as follows: control, cisplatin, isoflurane, cisplatin–isoflurane, halothane and cisplatin–halothane, and were exposed respectively for three consecutive days. The mice were treated with cisplatin or exposed to inhalation anaesthetic; the combined groups were exposed to inhalation anaesthetic after treatment with cisplatin. The alkaline comet assay was performed. All drugs had a strong genotoxicity (P < 0.05 vs. control group) in all of the observed cells. Isoflurane caused stronger DNA damage on the PBL and kidney cells, in contrast to halothane, which had stronger genotoxicity on brain and liver cells. The combination of cisplatin and isoflurane induced lower genotoxicity on PBL than isoflurane alone (P < 0.05). Halothane had the strongest effect on brain cells, but in the combined treatment with cisplatin, the effect decreased to the level of cisplatin alone. Halothane also induced the strongest DNA damage of the liver cells, while the combination with cisplatin increased its genotoxicity even more. The genotoxicity of cisplatin and isoflurane on kidney cells were nearly at the same level, but halothane caused a significantly lower effect. The combinations of inhalation anaesthetics with cisplatin had stronger effects on kidney cells than inhalation anaesthetics alone. The observed drugs and their combinations induced strong genotoxicity on all of the mentioned cells.</description><identifier>ISSN: 1234-1983</identifier><identifier>EISSN: 2190-3883</identifier><identifier>DOI: 10.1007/s13353-011-0046-0</identifier><identifier>PMID: 21509577</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>albino ; Anesthetics ; Anesthetics, Inhalation - administration & dosage ; Anesthetics, Inhalation - toxicity ; Animal Genetics and Genomics ; Animal Genetics ∙ Original Paper ; Animals ; Antimitotic agents ; Antineoplastic agents ; Biomedical and Life Sciences ; Brain ; Brain - cytology ; Brain - drug effects ; Cisplatin ; Cisplatin - administration & dosage ; Cisplatin - toxicity ; Comet assay ; Comet Assay - methods ; DNA damage ; DNA Damage - drug effects ; Drugs ; Ethylenediaminetetraacetic acid ; Genotoxicity ; Halides ; Halothane ; Halothane - administration & dosage ; Halothane - toxicity ; Hepatocytes ; Hepatocytes - drug effects ; Human Genetics ; Inhalation ; Isoflurane ; Isoflurane - administration & dosage ; Isoflurane - toxicity ; Kidney ; Kidney - cytology ; Kidney - drug effects ; kidney cells ; Leukocytes ; Leukocytes - cytology ; Leukocytes - drug effects ; Life Sciences ; Liver ; Liver diseases ; Male ; Mice ; Microbial Genetics and Genomics ; Mutagens - toxicity ; Peripheral blood ; Plant Genetics and Genomics</subject><ispartof>Journal of applied genetics, 2011-08, Vol.52 (3), p.355-361</ispartof><rights>Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2011</rights><rights>COPYRIGHT 2011 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-89467f827467c8a22c95f9321cc486c6987c502175c1e64dc66457a970b70c5e3</citedby><cites>FETCH-LOGICAL-c466t-89467f827467c8a22c95f9321cc486c6987c502175c1e64dc66457a970b70c5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13353-011-0046-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13353-011-0046-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21509577$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brozovic, Gordana</creatorcontrib><creatorcontrib>Orsolic, Nada</creatorcontrib><creatorcontrib>Knezevic, Fabijan</creatorcontrib><creatorcontrib>Horvat Knezevic, Anica</creatorcontrib><creatorcontrib>Benkovic, Vesna</creatorcontrib><creatorcontrib>Sakic, Katarina</creatorcontrib><creatorcontrib>Borojevic, Nikola</creatorcontrib><creatorcontrib>Dikic, Domagoj</creatorcontrib><title>in vivo genotoxicity of cisplatin, isoflurane and halothane evaluated by alkaline comet assay in Swiss albino mice</title><title>Journal of applied genetics</title><addtitle>J Appl Genetics</addtitle><addtitle>J Appl Genet</addtitle><description>The aim of this study was to evaluate the genotoxicity of repeated exposure to isoflurane or halothane and compare it with the genotoxicity of repeated exposure to cisplatin. We also determined the genotoxicity of combined treatment with inhalation anaesthetics and cisplatin on peripheral blood leucocytes (PBL), brain, liver and kidney cells of mice. The mice were divided into six groups as follows: control, cisplatin, isoflurane, cisplatin–isoflurane, halothane and cisplatin–halothane, and were exposed respectively for three consecutive days. The mice were treated with cisplatin or exposed to inhalation anaesthetic; the combined groups were exposed to inhalation anaesthetic after treatment with cisplatin. The alkaline comet assay was performed. All drugs had a strong genotoxicity (P < 0.05 vs. control group) in all of the observed cells. Isoflurane caused stronger DNA damage on the PBL and kidney cells, in contrast to halothane, which had stronger genotoxicity on brain and liver cells. The combination of cisplatin and isoflurane induced lower genotoxicity on PBL than isoflurane alone (P < 0.05). Halothane had the strongest effect on brain cells, but in the combined treatment with cisplatin, the effect decreased to the level of cisplatin alone. Halothane also induced the strongest DNA damage of the liver cells, while the combination with cisplatin increased its genotoxicity even more. The genotoxicity of cisplatin and isoflurane on kidney cells were nearly at the same level, but halothane caused a significantly lower effect. The combinations of inhalation anaesthetics with cisplatin had stronger effects on kidney cells than inhalation anaesthetics alone. The observed drugs and their combinations induced strong genotoxicity on all of the mentioned cells.</description><subject>albino</subject><subject>Anesthetics</subject><subject>Anesthetics, Inhalation - administration & dosage</subject><subject>Anesthetics, Inhalation - toxicity</subject><subject>Animal Genetics and Genomics</subject><subject>Animal Genetics ∙ Original Paper</subject><subject>Animals</subject><subject>Antimitotic agents</subject><subject>Antineoplastic agents</subject><subject>Biomedical and Life Sciences</subject><subject>Brain</subject><subject>Brain - cytology</subject><subject>Brain - drug effects</subject><subject>Cisplatin</subject><subject>Cisplatin - administration & dosage</subject><subject>Cisplatin - toxicity</subject><subject>Comet assay</subject><subject>Comet Assay - methods</subject><subject>DNA damage</subject><subject>DNA Damage - drug effects</subject><subject>Drugs</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Genotoxicity</subject><subject>Halides</subject><subject>Halothane</subject><subject>Halothane - administration & dosage</subject><subject>Halothane - toxicity</subject><subject>Hepatocytes</subject><subject>Hepatocytes - drug effects</subject><subject>Human Genetics</subject><subject>Inhalation</subject><subject>Isoflurane</subject><subject>Isoflurane - administration & dosage</subject><subject>Isoflurane - toxicity</subject><subject>Kidney</subject><subject>Kidney - cytology</subject><subject>Kidney - drug effects</subject><subject>kidney cells</subject><subject>Leukocytes</subject><subject>Leukocytes - cytology</subject><subject>Leukocytes - drug effects</subject><subject>Life Sciences</subject><subject>Liver</subject><subject>Liver diseases</subject><subject>Male</subject><subject>Mice</subject><subject>Microbial Genetics and Genomics</subject><subject>Mutagens - toxicity</subject><subject>Peripheral blood</subject><subject>Plant Genetics and Genomics</subject><issn>1234-1983</issn><issn>2190-3883</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kk1vFSEUhonR2NvqD3CjJC7cOJXPAZZNo9WkiYvaNeEycEtl4Aozt95_L5OpJibGsDjhnOc9OYcXAF5hdI4REh8qppTTDmHcIcT6Dj0BG4IV6qiU9CnYYEJZh5WkJ-C01nuEqGSCPAcnBHOkuBAbUEKCh3DIcOdSnvLPYMN0hNlDG-o-mimk9zDU7ONcTHLQpAHemZinu-XmDibOZnID3B6hid9NDC1r8-gmaGo1R9i63zyEWlt1G1KGY7DuBXjmTazu5WM8A7efPn67_Nxdf736cnlx3VnW91MnFeuFl0S0YKUhxCruFSXYWiZ72yspLEcEC26x69lg-55xYZRAW4Esd_QMvFv77kv-Mbs66TFU62Jso-e5aqkU5pIz0si3K7kz0emQfJ6KsQutLwRGgimlFur8H1Q7g2tr5eR8aPm_BHgV2JJrLc7rfQmjKUeNkV4M1KuBuhmoFwM1aprXj0PP29ENfxS_HWsAWYHaSmnnir7Pc0ntIf_b9c0q8iZrsyuh6tsbgjBD7U9woTD9BRVMraM</recordid><startdate>20110801</startdate><enddate>20110801</enddate><creator>Brozovic, Gordana</creator><creator>Orsolic, Nada</creator><creator>Knezevic, Fabijan</creator><creator>Horvat Knezevic, Anica</creator><creator>Benkovic, Vesna</creator><creator>Sakic, Katarina</creator><creator>Borojevic, Nikola</creator><creator>Dikic, Domagoj</creator><general>Springer-Verlag</general><general>Springer</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20110801</creationdate><title>in vivo genotoxicity of cisplatin, isoflurane and halothane evaluated by alkaline comet assay in Swiss albino mice</title><author>Brozovic, Gordana ; Orsolic, Nada ; Knezevic, Fabijan ; Horvat Knezevic, Anica ; Benkovic, Vesna ; Sakic, Katarina ; Borojevic, Nikola ; Dikic, Domagoj</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-89467f827467c8a22c95f9321cc486c6987c502175c1e64dc66457a970b70c5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>albino</topic><topic>Anesthetics</topic><topic>Anesthetics, Inhalation - administration & dosage</topic><topic>Anesthetics, Inhalation - toxicity</topic><topic>Animal Genetics and Genomics</topic><topic>Animal Genetics ∙ Original Paper</topic><topic>Animals</topic><topic>Antimitotic agents</topic><topic>Antineoplastic agents</topic><topic>Biomedical and Life Sciences</topic><topic>Brain</topic><topic>Brain - cytology</topic><topic>Brain - drug effects</topic><topic>Cisplatin</topic><topic>Cisplatin - administration & dosage</topic><topic>Cisplatin - toxicity</topic><topic>Comet assay</topic><topic>Comet Assay - methods</topic><topic>DNA damage</topic><topic>DNA Damage - drug effects</topic><topic>Drugs</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Genotoxicity</topic><topic>Halides</topic><topic>Halothane</topic><topic>Halothane - administration & dosage</topic><topic>Halothane - toxicity</topic><topic>Hepatocytes</topic><topic>Hepatocytes - drug effects</topic><topic>Human Genetics</topic><topic>Inhalation</topic><topic>Isoflurane</topic><topic>Isoflurane - administration & dosage</topic><topic>Isoflurane - toxicity</topic><topic>Kidney</topic><topic>Kidney - cytology</topic><topic>Kidney - drug effects</topic><topic>kidney cells</topic><topic>Leukocytes</topic><topic>Leukocytes - cytology</topic><topic>Leukocytes - drug effects</topic><topic>Life Sciences</topic><topic>Liver</topic><topic>Liver diseases</topic><topic>Male</topic><topic>Mice</topic><topic>Microbial Genetics and Genomics</topic><topic>Mutagens - toxicity</topic><topic>Peripheral blood</topic><topic>Plant Genetics and Genomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brozovic, Gordana</creatorcontrib><creatorcontrib>Orsolic, Nada</creatorcontrib><creatorcontrib>Knezevic, Fabijan</creatorcontrib><creatorcontrib>Horvat Knezevic, Anica</creatorcontrib><creatorcontrib>Benkovic, Vesna</creatorcontrib><creatorcontrib>Sakic, Katarina</creatorcontrib><creatorcontrib>Borojevic, Nikola</creatorcontrib><creatorcontrib>Dikic, Domagoj</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brozovic, Gordana</au><au>Orsolic, Nada</au><au>Knezevic, Fabijan</au><au>Horvat Knezevic, Anica</au><au>Benkovic, Vesna</au><au>Sakic, Katarina</au><au>Borojevic, Nikola</au><au>Dikic, Domagoj</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>in vivo genotoxicity of cisplatin, isoflurane and halothane evaluated by alkaline comet assay in Swiss albino mice</atitle><jtitle>Journal of applied genetics</jtitle><stitle>J Appl Genetics</stitle><addtitle>J Appl Genet</addtitle><date>2011-08-01</date><risdate>2011</risdate><volume>52</volume><issue>3</issue><spage>355</spage><epage>361</epage><pages>355-361</pages><issn>1234-1983</issn><eissn>2190-3883</eissn><abstract>The aim of this study was to evaluate the genotoxicity of repeated exposure to isoflurane or halothane and compare it with the genotoxicity of repeated exposure to cisplatin. We also determined the genotoxicity of combined treatment with inhalation anaesthetics and cisplatin on peripheral blood leucocytes (PBL), brain, liver and kidney cells of mice. The mice were divided into six groups as follows: control, cisplatin, isoflurane, cisplatin–isoflurane, halothane and cisplatin–halothane, and were exposed respectively for three consecutive days. The mice were treated with cisplatin or exposed to inhalation anaesthetic; the combined groups were exposed to inhalation anaesthetic after treatment with cisplatin. The alkaline comet assay was performed. All drugs had a strong genotoxicity (P < 0.05 vs. control group) in all of the observed cells. Isoflurane caused stronger DNA damage on the PBL and kidney cells, in contrast to halothane, which had stronger genotoxicity on brain and liver cells. The combination of cisplatin and isoflurane induced lower genotoxicity on PBL than isoflurane alone (P < 0.05). Halothane had the strongest effect on brain cells, but in the combined treatment with cisplatin, the effect decreased to the level of cisplatin alone. Halothane also induced the strongest DNA damage of the liver cells, while the combination with cisplatin increased its genotoxicity even more. The genotoxicity of cisplatin and isoflurane on kidney cells were nearly at the same level, but halothane caused a significantly lower effect. The combinations of inhalation anaesthetics with cisplatin had stronger effects on kidney cells than inhalation anaesthetics alone. The observed drugs and their combinations induced strong genotoxicity on all of the mentioned cells.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21509577</pmid><doi>10.1007/s13353-011-0046-0</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1234-1983 |
| ispartof | Journal of applied genetics, 2011-08, Vol.52 (3), p.355-361 |
| issn | 1234-1983 2190-3883 |
| language | eng |
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| subjects | albino Anesthetics Anesthetics, Inhalation - administration & dosage Anesthetics, Inhalation - toxicity Animal Genetics and Genomics Animal Genetics ∙ Original Paper Animals Antimitotic agents Antineoplastic agents Biomedical and Life Sciences Brain Brain - cytology Brain - drug effects Cisplatin Cisplatin - administration & dosage Cisplatin - toxicity Comet assay Comet Assay - methods DNA damage DNA Damage - drug effects Drugs Ethylenediaminetetraacetic acid Genotoxicity Halides Halothane Halothane - administration & dosage Halothane - toxicity Hepatocytes Hepatocytes - drug effects Human Genetics Inhalation Isoflurane Isoflurane - administration & dosage Isoflurane - toxicity Kidney Kidney - cytology Kidney - drug effects kidney cells Leukocytes Leukocytes - cytology Leukocytes - drug effects Life Sciences Liver Liver diseases Male Mice Microbial Genetics and Genomics Mutagens - toxicity Peripheral blood Plant Genetics and Genomics |
| title | in vivo genotoxicity of cisplatin, isoflurane and halothane evaluated by alkaline comet assay in Swiss albino mice |
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