Establishment of Clinically Compliant Human Embryonic Stem Cells in an Autologous Feeder-Free System

Applications of human embryonic stem cells (hESCs) are limited by the use of mouse embryonic fibroblasts feeder and animal-derived components during culture. In this study, we demonstrated the potential use of extracellular matrix (ECM) derived from the autologous feeders to support long-term undiff...

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Veröffentlicht in:	Tissue engineering. Part C, Methods Methods, 2011-09, Vol.17 (9), p.927-937
Hauptverfasser:	Fu, Xin, Toh, Wei Seong, Liu, Hua, Lu, Kai, Li, Mingming, Cao, Tong
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell culture Cell Culture Techniques - methods Cell Differentiation - genetics Cell Line Cell Proliferation Cell Shape Embryoid Bodies - cytology Embryoid Bodies - metabolism Embryonic stem cells Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Embryos Extracellular Matrix - metabolism Feeder Cells - cytology Feeder Cells - metabolism Fibroblasts - cytology Flow Cytometry Gene expression Gene Expression Profiling Gene Expression Regulation Genetic aspects Genetic markers Humans Karyotyping Mice Physiological aspects Pluripotent Stem Cells - cytology Pluripotent Stem Cells - metabolism Real-Time Polymerase Chain Reaction Stem cells Tissue engineering
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