Xpert MTB/RIF registered , a novel automated polymerase chain reaction-based tool for the diagnosis of tuberculosis
There is an urgent need for new point of care tests for tuberculosis (TB). Xpert MTB/RIF registered is a real-time polymerase chain reaction-based system that detects Mycobacterium tuberculosis DNA and rifampicin (RMP) resistance modulating mutations directly from clinical samples in 2 h. The sensit...
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| Veröffentlicht in: | The international journal of tuberculosis and lung disease 2011-07, Vol.15 (7), p.988-989 |
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| container_issue | 7 |
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| container_title | The international journal of tuberculosis and lung disease |
| container_volume | 15 |
| creator | Bowles, E C Freyee, B van Ingen, J Mulder, B Boeree, MJ van Soolingen, D |
| description | There is an urgent need for new point of care tests for tuberculosis (TB). Xpert MTB/RIF registered is a real-time polymerase chain reaction-based system that detects Mycobacterium tuberculosis DNA and rifampicin (RMP) resistance modulating mutations directly from clinical samples in 2 h. The sensitivity for detecting M. tuberculosis in culture-positive samples was 93.8% (60/64) and exceeded smear microscopy (40/64, 62.5%). The specificity for detecting M. tuberculosis was 92.0% (23/25) and for RMP resistance it was 100% (8/8). The test is simple to conduct and requires basic sputum handling facilities only. These characteristics render it a promising close-to-patient test for TB in various settings. |
| doi_str_mv | 10.5588/ijtld.10.0574 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1027-3719 |
| ispartof | The international journal of tuberculosis and lung disease, 2011-07, Vol.15 (7), p.988-989 |
| issn | 1027-3719 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_899153360 |
| source | Alma/SFX Local Collection |
| subjects | Mycobacterium tuberculosis |
| title | Xpert MTB/RIF registered , a novel automated polymerase chain reaction-based tool for the diagnosis of tuberculosis |
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