High level phytase production by Aspergillus niger NCIM 563 in solid state culture: response surface optimization, up-scaling, and its partial characterization
Phytase production by Aspergillus niger NCIM 563 was optimized by using wheat bran in solid state fermentation (SSF). An integrated statistical optimization approach involving the combination of Placket–Burman design (PBD) and Box–Behnken design (BBD) was employed. PBD was used to evaluate the effec...
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| description | Phytase production by Aspergillus niger NCIM 563 was optimized by using wheat bran in solid state fermentation (SSF). An integrated statistical optimization approach involving the combination of Placket–Burman design (PBD) and Box–Behnken design (BBD) was employed. PBD was used to evaluate the effect of 11 variables related to phytase production, and five statistically significant variables, namely, glucose, dextrin, NaNO3, distilled water, and MgSO4·7H2O, were selected for further optimization studies. The levels of five variables for maximum phytase production were determined by a BBD. Phytase production improved from 50 IU/g dry moldy bran (DMB) to 154 IU/g DMB indicating 3.08-fold increase after optimization. A simultaneous reduction in fermentation time from 7 to 4 days shows a high productivity of 38,500 IU/kg/day. Scaling up the process in trays gave reproducible phytase production overcoming industrial constraints of practicability and economics. The culture extract also had 133.2, 41.58, and 310.34 IU/g DMB of xylanase, cellulase, and amylase activities, respectively. The partially purified phytase was optimally active at 55°C and pH 6.0. The enzyme retained ca. 75% activity over a wide pH range 2.0–9.5. It also released more inorganic phosphorus from soybean meal in a broad pH range from 2.5 to 6.5 under emulated gastric conditions. Molecular weight of phytase on Sephacryl S-200 was approximately 87 kDa. The K m and V max observed were 0.156 mM and 220 μm/min/mg. The SSF phytase from A. niger NCIM 563 offers an economical production capability and its wide pH stability shows its suitability for use in poultry feed. |
| doi_str_mv | 10.1007/s10295-010-0926-z |
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M</creator><creatorcontrib>Bhavsar, K ; Ravi Kumar, V ; Khire, J. M</creatorcontrib><description>Phytase production by Aspergillus niger NCIM 563 was optimized by using wheat bran in solid state fermentation (SSF). An integrated statistical optimization approach involving the combination of Placket–Burman design (PBD) and Box–Behnken design (BBD) was employed. PBD was used to evaluate the effect of 11 variables related to phytase production, and five statistically significant variables, namely, glucose, dextrin, NaNO3, distilled water, and MgSO4·7H2O, were selected for further optimization studies. The levels of five variables for maximum phytase production were determined by a BBD. Phytase production improved from 50 IU/g dry moldy bran (DMB) to 154 IU/g DMB indicating 3.08-fold increase after optimization. A simultaneous reduction in fermentation time from 7 to 4 days shows a high productivity of 38,500 IU/kg/day. Scaling up the process in trays gave reproducible phytase production overcoming industrial constraints of practicability and economics. The culture extract also had 133.2, 41.58, and 310.34 IU/g DMB of xylanase, cellulase, and amylase activities, respectively. The partially purified phytase was optimally active at 55°C and pH 6.0. The enzyme retained ca. 75% activity over a wide pH range 2.0–9.5. It also released more inorganic phosphorus from soybean meal in a broad pH range from 2.5 to 6.5 under emulated gastric conditions. Molecular weight of phytase on Sephacryl S-200 was approximately 87 kDa. The K m and V max observed were 0.156 mM and 220 μm/min/mg. The SSF phytase from A. niger NCIM 563 offers an economical production capability and its wide pH stability shows its suitability for use in poultry feed.</description><identifier>ISSN: 1367-5435</identifier><identifier>EISSN: 1476-5535</identifier><identifier>DOI: 10.1007/s10295-010-0926-z</identifier><identifier>PMID: 21184251</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>6-Phytase - biosynthesis ; 6-Phytase - isolation & purification ; 6-Phytase - metabolism ; Agricultural biotechnology ; Animals ; Aspergillus niger ; Aspergillus niger - enzymology ; Biochemistry ; Bioinformatics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Cellulase ; Culture Media - chemistry ; Dietary fiber ; Dietary Fiber - microbiology ; Distilled water ; economics ; endo-1,4-beta-glucanase ; Enzymes ; Feeds ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Fungi ; Genetic Engineering ; glucose ; Inorganic Chemistry ; inorganic phosphorus ; Laboratories ; Life Sciences ; Methods. Procedures. Technologies ; Microbial engineering. Fermentation and microbial culture technology ; Microbiology ; molecular weight ; Optimization techniques ; Original Paper ; Phosphate esters ; Phosphorus ; phytases ; Poultry ; Poultry feed ; solid state fermentation ; soybean meal ; Soybeans ; Statistical methods ; Studies ; trays ; Triticum aestivum ; Variables ; Wheat bran ; xylanases</subject><ispartof>Journal of industrial microbiology & biotechnology, 2011-09, Vol.38 (9), p.1407-1417</ispartof><rights>Society for Industrial Microbiology 2010</rights><rights>2015 INIST-CNRS</rights><rights>Society for Industrial Microbiology 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-d51cd07729c8f5d787f9f2bb7d95f56320423b5c7d068212c16dae16fa1ece843</citedby><cites>FETCH-LOGICAL-c536t-d51cd07729c8f5d787f9f2bb7d95f56320423b5c7d068212c16dae16fa1ece843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10295-010-0926-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10295-010-0926-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24501613$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21184251$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bhavsar, K</creatorcontrib><creatorcontrib>Ravi Kumar, V</creatorcontrib><creatorcontrib>Khire, J. M</creatorcontrib><title>High level phytase production by Aspergillus niger NCIM 563 in solid state culture: response surface optimization, up-scaling, and its partial characterization</title><title>Journal of industrial microbiology & biotechnology</title><addtitle>J Ind Microbiol Biotechnol</addtitle><addtitle>J Ind Microbiol Biotechnol</addtitle><description>Phytase production by Aspergillus niger NCIM 563 was optimized by using wheat bran in solid state fermentation (SSF). An integrated statistical optimization approach involving the combination of Placket–Burman design (PBD) and Box–Behnken design (BBD) was employed. PBD was used to evaluate the effect of 11 variables related to phytase production, and five statistically significant variables, namely, glucose, dextrin, NaNO3, distilled water, and MgSO4·7H2O, were selected for further optimization studies. The levels of five variables for maximum phytase production were determined by a BBD. Phytase production improved from 50 IU/g dry moldy bran (DMB) to 154 IU/g DMB indicating 3.08-fold increase after optimization. A simultaneous reduction in fermentation time from 7 to 4 days shows a high productivity of 38,500 IU/kg/day. Scaling up the process in trays gave reproducible phytase production overcoming industrial constraints of practicability and economics. The culture extract also had 133.2, 41.58, and 310.34 IU/g DMB of xylanase, cellulase, and amylase activities, respectively. The partially purified phytase was optimally active at 55°C and pH 6.0. The enzyme retained ca. 75% activity over a wide pH range 2.0–9.5. It also released more inorganic phosphorus from soybean meal in a broad pH range from 2.5 to 6.5 under emulated gastric conditions. Molecular weight of phytase on Sephacryl S-200 was approximately 87 kDa. The K m and V max observed were 0.156 mM and 220 μm/min/mg. The SSF phytase from A. niger NCIM 563 offers an economical production capability and its wide pH stability shows its suitability for use in poultry feed.</description><subject>6-Phytase - biosynthesis</subject><subject>6-Phytase - isolation & purification</subject><subject>6-Phytase - metabolism</subject><subject>Agricultural biotechnology</subject><subject>Animals</subject><subject>Aspergillus niger</subject><subject>Aspergillus niger - enzymology</subject><subject>Biochemistry</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cellulase</subject><subject>Culture Media - chemistry</subject><subject>Dietary fiber</subject><subject>Dietary Fiber - microbiology</subject><subject>Distilled water</subject><subject>economics</subject><subject>endo-1,4-beta-glucanase</subject><subject>Enzymes</subject><subject>Feeds</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi</subject><subject>Genetic Engineering</subject><subject>glucose</subject><subject>Inorganic Chemistry</subject><subject>inorganic phosphorus</subject><subject>Laboratories</subject><subject>Life Sciences</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. Fermentation and microbial culture technology</subject><subject>Microbiology</subject><subject>molecular weight</subject><subject>Optimization techniques</subject><subject>Original Paper</subject><subject>Phosphate esters</subject><subject>Phosphorus</subject><subject>phytases</subject><subject>Poultry</subject><subject>Poultry feed</subject><subject>solid state fermentation</subject><subject>soybean meal</subject><subject>Soybeans</subject><subject>Statistical methods</subject><subject>Studies</subject><subject>trays</subject><subject>Triticum aestivum</subject><subject>Variables</subject><subject>Wheat bran</subject><subject>xylanases</subject><issn>1367-5435</issn><issn>1476-5535</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFks1u1DAUhSMEoqXwAGzAQkJsGvD1T2Kzq0ZAKxVYQNeR49gZV54k2A7SzMvwqjhkoBILWNmSv3vuPfe4KJ4Cfg0Y128iYCJ5iQGXWJKqPNwrToHVVck55ffznVZ1yRnlJ8WjGG8xxryuycPihAAIRjicFj8uXb9F3nw3Hk3bfVLRoCmM3ayTGwfU7tFFnEzonfdzRIPrTUCfNlcfEa8ocgOKo3cdikklg_Ts0xzMWxRMnMYhK8U5WKUNGqfkdu6gFs1zNE9l1Mq7oT9HauiQSxFNKiSnPNJbFZROJhzpx8UDq3w0T47nWXHz_t3XzWV5_fnD1ebiutScVqnsOOgOZ3NSC8u7WtRWWtK2dSe5zaMSzAhtua47XAkCREPVKQOVVWC0EYyeFa9W3Wz-22xianYuauO9Gsw4x0ZICRwEpv8nBSeyolJk8sVf5O04hyHbyBATnAMscrBCOowxBmObKbidCvsGcLOk3KwpNznlZkm5OeSaZ0fhud2Z7k_F71gz8PIIqGXTNqhBu3jHMY6h-tWcrFzMT0PO9m7Cf3V_vhZZNTaqD1n45gvBwPL_YlIAoz8BpoLKcA</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Bhavsar, K</creator><creator>Ravi Kumar, V</creator><creator>Khire, J. 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M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-d51cd07729c8f5d787f9f2bb7d95f56320423b5c7d068212c16dae16fa1ece843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>6-Phytase - biosynthesis</topic><topic>6-Phytase - isolation & purification</topic><topic>6-Phytase - metabolism</topic><topic>Agricultural biotechnology</topic><topic>Animals</topic><topic>Aspergillus niger</topic><topic>Aspergillus niger - enzymology</topic><topic>Biochemistry</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cellulase</topic><topic>Culture Media - chemistry</topic><topic>Dietary fiber</topic><topic>Dietary Fiber - microbiology</topic><topic>Distilled water</topic><topic>economics</topic><topic>endo-1,4-beta-glucanase</topic><topic>Enzymes</topic><topic>Feeds</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungi</topic><topic>Genetic Engineering</topic><topic>glucose</topic><topic>Inorganic Chemistry</topic><topic>inorganic phosphorus</topic><topic>Laboratories</topic><topic>Life Sciences</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial engineering. Fermentation and microbial culture technology</topic><topic>Microbiology</topic><topic>molecular weight</topic><topic>Optimization techniques</topic><topic>Original Paper</topic><topic>Phosphate esters</topic><topic>Phosphorus</topic><topic>phytases</topic><topic>Poultry</topic><topic>Poultry feed</topic><topic>solid state fermentation</topic><topic>soybean meal</topic><topic>Soybeans</topic><topic>Statistical methods</topic><topic>Studies</topic><topic>trays</topic><topic>Triticum aestivum</topic><topic>Variables</topic><topic>Wheat bran</topic><topic>xylanases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bhavsar, K</creatorcontrib><creatorcontrib>Ravi Kumar, V</creatorcontrib><creatorcontrib>Khire, J. 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M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High level phytase production by Aspergillus niger NCIM 563 in solid state culture: response surface optimization, up-scaling, and its partial characterization</atitle><jtitle>Journal of industrial microbiology & biotechnology</jtitle><stitle>J Ind Microbiol Biotechnol</stitle><addtitle>J Ind Microbiol Biotechnol</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>38</volume><issue>9</issue><spage>1407</spage><epage>1417</epage><pages>1407-1417</pages><issn>1367-5435</issn><eissn>1476-5535</eissn><abstract>Phytase production by Aspergillus niger NCIM 563 was optimized by using wheat bran in solid state fermentation (SSF). An integrated statistical optimization approach involving the combination of Placket–Burman design (PBD) and Box–Behnken design (BBD) was employed. PBD was used to evaluate the effect of 11 variables related to phytase production, and five statistically significant variables, namely, glucose, dextrin, NaNO3, distilled water, and MgSO4·7H2O, were selected for further optimization studies. The levels of five variables for maximum phytase production were determined by a BBD. Phytase production improved from 50 IU/g dry moldy bran (DMB) to 154 IU/g DMB indicating 3.08-fold increase after optimization. A simultaneous reduction in fermentation time from 7 to 4 days shows a high productivity of 38,500 IU/kg/day. Scaling up the process in trays gave reproducible phytase production overcoming industrial constraints of practicability and economics. The culture extract also had 133.2, 41.58, and 310.34 IU/g DMB of xylanase, cellulase, and amylase activities, respectively. The partially purified phytase was optimally active at 55°C and pH 6.0. The enzyme retained ca. 75% activity over a wide pH range 2.0–9.5. It also released more inorganic phosphorus from soybean meal in a broad pH range from 2.5 to 6.5 under emulated gastric conditions. Molecular weight of phytase on Sephacryl S-200 was approximately 87 kDa. The K m and V max observed were 0.156 mM and 220 μm/min/mg. The SSF phytase from A. niger NCIM 563 offers an economical production capability and its wide pH stability shows its suitability for use in poultry feed.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21184251</pmid><doi>10.1007/s10295-010-0926-z</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 6-Phytase - biosynthesis 6-Phytase - isolation & purification 6-Phytase - metabolism Agricultural biotechnology Animals Aspergillus niger Aspergillus niger - enzymology Biochemistry Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biotechnology Cellulase Culture Media - chemistry Dietary fiber Dietary Fiber - microbiology Distilled water economics endo-1,4-beta-glucanase Enzymes Feeds Fermentation Fundamental and applied biological sciences. Psychology Fungi Genetic Engineering glucose Inorganic Chemistry inorganic phosphorus Laboratories Life Sciences Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Microbiology molecular weight Optimization techniques Original Paper Phosphate esters Phosphorus phytases Poultry Poultry feed solid state fermentation soybean meal Soybeans Statistical methods Studies trays Triticum aestivum Variables Wheat bran xylanases |
| title | High level phytase production by Aspergillus niger NCIM 563 in solid state culture: response surface optimization, up-scaling, and its partial characterization |
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