Agrobacterium-mediated transformation of leaf base derived callus tissues of popular indica rice ( Oryza sativa L. sub sp. indica cv. ADT 43)
[Display omitted] ► Popular ADT 43 indica rice having recalcitrant regeneration system. ► 28 days old leaf base derived calli were used for co-cultivation. ► Carbenicillin, cefotaxime and timentin are evaluated to eliminate A. tumefaciens. ► No reports available on the influence of antibiotic timent...
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| Veröffentlicht in: | Plant science (Limerick) 2011-09, Vol.181 (3), p.258-268 |
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| creator | Karthikeyan, Alagarsamy Pandian, Shunmugiah Karutha Ramesh, Manikandan |
| description | [Display omitted]
► Popular ADT 43
indica rice having recalcitrant regeneration system. ► 28 days old leaf base derived calli were used for co-cultivation. ► Carbenicillin, cefotaxime and timentin are evaluated to eliminate
A. tumefaciens.
► No reports available on the influence of antibiotic timentin on rice regeneration.
A simple and efficient protocol for the
Agrobacterium-mediated transformation of an agronomically useful abiotic sensitive popular
indica rice cv. ADT 43 has been developed. Initiation of calli were best achieved from the leaf bases of 4 days old rice seedlings on LS medium supplemented with 2.5
mg/L 2,4-D and 1.0
mg/L thiamine–HCl. Rice calli immersed in
Agrobacterium suspension (strain EHA 105, OD
600
=
0.8) were co-cultured on LS30-AsPC medium for 2 days at 25
±
2
°C in the dark. Based on GUS expression analysis, 10
min co-cultivation time with 100
μM acetosyringone was found optimum for the delivery of
gus gene. Calli were proved to be very sensitive to
Agrobacterium infection and we found that the level of necrotic response can be minimized after co-cultivation with 30% LS, 10
g/L PVP, 10% coconut water and 250
mg/L timentin which improved the final transformation efficiency to 9.33%. Molecular and genetic analysis of transgenic plants reveals the integration, expression and inheritance of transgene in the progeny (T
1) of these plants. The copy number of transgenes has been found to vary from 1 to 2 in transgenic plants (T
0 and T
1). |
| doi_str_mv | 10.1016/j.plantsci.2011.05.011 |
| format | Article |
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► Popular ADT 43
indica rice having recalcitrant regeneration system. ► 28 days old leaf base derived calli were used for co-cultivation. ► Carbenicillin, cefotaxime and timentin are evaluated to eliminate
A. tumefaciens.
► No reports available on the influence of antibiotic timentin on rice regeneration.
A simple and efficient protocol for the
Agrobacterium-mediated transformation of an agronomically useful abiotic sensitive popular
indica rice cv. ADT 43 has been developed. Initiation of calli were best achieved from the leaf bases of 4 days old rice seedlings on LS medium supplemented with 2.5
mg/L 2,4-D and 1.0
mg/L thiamine–HCl. Rice calli immersed in
Agrobacterium suspension (strain EHA 105, OD
600
=
0.8) were co-cultured on LS30-AsPC medium for 2 days at 25
±
2
°C in the dark. Based on GUS expression analysis, 10
min co-cultivation time with 100
μM acetosyringone was found optimum for the delivery of
gus gene. Calli were proved to be very sensitive to
Agrobacterium infection and we found that the level of necrotic response can be minimized after co-cultivation with 30% LS, 10
g/L PVP, 10% coconut water and 250
mg/L timentin which improved the final transformation efficiency to 9.33%. Molecular and genetic analysis of transgenic plants reveals the integration, expression and inheritance of transgene in the progeny (T
1) of these plants. The copy number of transgenes has been found to vary from 1 to 2 in transgenic plants (T
0 and T
1).</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/j.plantsci.2011.05.011</identifier><identifier>PMID: 21763536</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>2,4-D ; Abiotic factors ; Acetophenones - administration & dosage ; Acetosyringone ; Agrobacterium ; Biological and medical sciences ; callus ; Cell Culture Techniques - methods ; Coconut water ; coconuts ; Culture Media - chemistry ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant ; Genes, Plant ; GUS (β-glucuronidase) ; HPT (hygromycin phosphotransferase) ; inheritance (genetics) ; leaves ; Oryza - genetics ; Oryza - growth & development ; Oryza - microbiology ; Oryza sativa ; Plant Growth Regulators - metabolism ; Plant Leaves - genetics ; Plant Leaves - growth & development ; Plant Leaves - microbiology ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - growth & development ; Plants, Genetically Modified - microbiology ; Polyvinyl pyrrolidone (PVP) ; progeny ; Rhizobium - genetics ; rice ; seedlings ; Transformation, Genetic ; Transgenes ; transgenic plants</subject><ispartof>Plant science (Limerick), 2011-09, Vol.181 (3), p.258-268</ispartof><rights>2011 Elsevier Ireland Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-ef9b39f78426488689b4b66a31ca943a9ea3ec80c215d008133c1ee481477d103</citedby><cites>FETCH-LOGICAL-c453t-ef9b39f78426488689b4b66a31ca943a9ea3ec80c215d008133c1ee481477d103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168945211001580$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24361772$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21763536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karthikeyan, Alagarsamy</creatorcontrib><creatorcontrib>Pandian, Shunmugiah Karutha</creatorcontrib><creatorcontrib>Ramesh, Manikandan</creatorcontrib><title>Agrobacterium-mediated transformation of leaf base derived callus tissues of popular indica rice ( Oryza sativa L. sub sp. indica cv. ADT 43)</title><title>Plant science (Limerick)</title><addtitle>Plant Sci</addtitle><description>[Display omitted]
► Popular ADT 43
indica rice having recalcitrant regeneration system. ► 28 days old leaf base derived calli were used for co-cultivation. ► Carbenicillin, cefotaxime and timentin are evaluated to eliminate
A. tumefaciens.
► No reports available on the influence of antibiotic timentin on rice regeneration.
A simple and efficient protocol for the
Agrobacterium-mediated transformation of an agronomically useful abiotic sensitive popular
indica rice cv. ADT 43 has been developed. Initiation of calli were best achieved from the leaf bases of 4 days old rice seedlings on LS medium supplemented with 2.5
mg/L 2,4-D and 1.0
mg/L thiamine–HCl. Rice calli immersed in
Agrobacterium suspension (strain EHA 105, OD
600
=
0.8) were co-cultured on LS30-AsPC medium for 2 days at 25
±
2
°C in the dark. Based on GUS expression analysis, 10
min co-cultivation time with 100
μM acetosyringone was found optimum for the delivery of
gus gene. Calli were proved to be very sensitive to
Agrobacterium infection and we found that the level of necrotic response can be minimized after co-cultivation with 30% LS, 10
g/L PVP, 10% coconut water and 250
mg/L timentin which improved the final transformation efficiency to 9.33%. Molecular and genetic analysis of transgenic plants reveals the integration, expression and inheritance of transgene in the progeny (T
1) of these plants. The copy number of transgenes has been found to vary from 1 to 2 in transgenic plants (T
0 and T
1).</description><subject>2,4-D</subject><subject>Abiotic factors</subject><subject>Acetophenones - administration & dosage</subject><subject>Acetosyringone</subject><subject>Agrobacterium</subject><subject>Biological and medical sciences</subject><subject>callus</subject><subject>Cell Culture Techniques - methods</subject><subject>Coconut water</subject><subject>coconuts</subject><subject>Culture Media - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes, Plant</subject><subject>GUS (β-glucuronidase)</subject><subject>HPT (hygromycin phosphotransferase)</subject><subject>inheritance (genetics)</subject><subject>leaves</subject><subject>Oryza - genetics</subject><subject>Oryza - growth & development</subject><subject>Oryza - microbiology</subject><subject>Oryza sativa</subject><subject>Plant Growth Regulators - metabolism</subject><subject>Plant Leaves - genetics</subject><subject>Plant Leaves - growth & development</subject><subject>Plant Leaves - microbiology</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - growth & development</subject><subject>Plants, Genetically Modified - microbiology</subject><subject>Polyvinyl pyrrolidone (PVP)</subject><subject>progeny</subject><subject>Rhizobium - genetics</subject><subject>rice</subject><subject>seedlings</subject><subject>Transformation, Genetic</subject><subject>Transgenes</subject><subject>transgenic plants</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0ctu1DAUBuAIgehQeIXiDQIWCb7FsXcdlas0Uhe0a-vEcSqPknGwk5HKO_DOnNHMwLKrs_nORecviitGK0aZ-rStpgF2c3ah4pSxitYVlmfFiulGlJzX5nmxQqhLI2t-UbzKeUsp5XXdvCwuOGuUqIVaFX_WDym24GafwjKWo-8CzL4jc4Jd7mMaYQ5xR2JPBg89aSF70qHdo3EwDEsmc8h58flgpjgtAyQSdl1wQFJwnnwgt-nxN5CMk_ZANhXJS0vyVJ2V21dk_fmOSPHxdfGihyH7N6d6Wdx__XJ3873c3H77cbPelE7WYi59b1ph-kZLrqTWSptWtkqBYA6MFGA8CO80dZzVHaWaCeGY91Iz2TQdo-KyeH-cO6X4C2-f7Riy8wO-1MclW20MUs3F07LRlBtBNUp1lC7FnJPv7ZTCCOnRMmoPmdmtPWdmD5lZWlss2Hh1WrG0-P9_beeQELw7Acj48x6jcSH_d1Io1jQc3duj6yFaeEho7n_iJonBM2V0jeL6KDw-dx98sniM3zlMPXk32y6Gp679CyoewI8</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Karthikeyan, Alagarsamy</creator><creator>Pandian, Shunmugiah Karutha</creator><creator>Ramesh, Manikandan</creator><general>Elsevier Ireland Ltd</general><general>[Ireland]: Elsevier Science Ireland Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20110901</creationdate><title>Agrobacterium-mediated transformation of leaf base derived callus tissues of popular indica rice ( Oryza sativa L. sub sp. indica cv. ADT 43)</title><author>Karthikeyan, Alagarsamy ; Pandian, Shunmugiah Karutha ; Ramesh, Manikandan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-ef9b39f78426488689b4b66a31ca943a9ea3ec80c215d008133c1ee481477d103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>2,4-D</topic><topic>Abiotic factors</topic><topic>Acetophenones - administration & dosage</topic><topic>Acetosyringone</topic><topic>Agrobacterium</topic><topic>Biological and medical sciences</topic><topic>callus</topic><topic>Cell Culture Techniques - methods</topic><topic>Coconut water</topic><topic>coconuts</topic><topic>Culture Media - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes, Plant</topic><topic>GUS (β-glucuronidase)</topic><topic>HPT (hygromycin phosphotransferase)</topic><topic>inheritance (genetics)</topic><topic>leaves</topic><topic>Oryza - genetics</topic><topic>Oryza - growth & development</topic><topic>Oryza - microbiology</topic><topic>Oryza sativa</topic><topic>Plant Growth Regulators - metabolism</topic><topic>Plant Leaves - genetics</topic><topic>Plant Leaves - growth & development</topic><topic>Plant Leaves - microbiology</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - growth & development</topic><topic>Plants, Genetically Modified - microbiology</topic><topic>Polyvinyl pyrrolidone (PVP)</topic><topic>progeny</topic><topic>Rhizobium - genetics</topic><topic>rice</topic><topic>seedlings</topic><topic>Transformation, Genetic</topic><topic>Transgenes</topic><topic>transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karthikeyan, Alagarsamy</creatorcontrib><creatorcontrib>Pandian, Shunmugiah Karutha</creatorcontrib><creatorcontrib>Ramesh, Manikandan</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karthikeyan, Alagarsamy</au><au>Pandian, Shunmugiah Karutha</au><au>Ramesh, Manikandan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Agrobacterium-mediated transformation of leaf base derived callus tissues of popular indica rice ( Oryza sativa L. sub sp. indica cv. ADT 43)</atitle><jtitle>Plant science (Limerick)</jtitle><addtitle>Plant Sci</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>181</volume><issue>3</issue><spage>258</spage><epage>268</epage><pages>258-268</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><coden>PLSCE4</coden><abstract>[Display omitted]
► Popular ADT 43
indica rice having recalcitrant regeneration system. ► 28 days old leaf base derived calli were used for co-cultivation. ► Carbenicillin, cefotaxime and timentin are evaluated to eliminate
A. tumefaciens.
► No reports available on the influence of antibiotic timentin on rice regeneration.
A simple and efficient protocol for the
Agrobacterium-mediated transformation of an agronomically useful abiotic sensitive popular
indica rice cv. ADT 43 has been developed. Initiation of calli were best achieved from the leaf bases of 4 days old rice seedlings on LS medium supplemented with 2.5
mg/L 2,4-D and 1.0
mg/L thiamine–HCl. Rice calli immersed in
Agrobacterium suspension (strain EHA 105, OD
600
=
0.8) were co-cultured on LS30-AsPC medium for 2 days at 25
±
2
°C in the dark. Based on GUS expression analysis, 10
min co-cultivation time with 100
μM acetosyringone was found optimum for the delivery of
gus gene. Calli were proved to be very sensitive to
Agrobacterium infection and we found that the level of necrotic response can be minimized after co-cultivation with 30% LS, 10
g/L PVP, 10% coconut water and 250
mg/L timentin which improved the final transformation efficiency to 9.33%. Molecular and genetic analysis of transgenic plants reveals the integration, expression and inheritance of transgene in the progeny (T
1) of these plants. The copy number of transgenes has been found to vary from 1 to 2 in transgenic plants (T
0 and T
1).</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>21763536</pmid><doi>10.1016/j.plantsci.2011.05.011</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-9452 |
| ispartof | Plant science (Limerick), 2011-09, Vol.181 (3), p.258-268 |
| issn | 0168-9452 1873-2259 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 2,4-D Abiotic factors Acetophenones - administration & dosage Acetosyringone Agrobacterium Biological and medical sciences callus Cell Culture Techniques - methods Coconut water coconuts Culture Media - chemistry Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant Genes, Plant GUS (β-glucuronidase) HPT (hygromycin phosphotransferase) inheritance (genetics) leaves Oryza - genetics Oryza - growth & development Oryza - microbiology Oryza sativa Plant Growth Regulators - metabolism Plant Leaves - genetics Plant Leaves - growth & development Plant Leaves - microbiology Plants, Genetically Modified - genetics Plants, Genetically Modified - growth & development Plants, Genetically Modified - microbiology Polyvinyl pyrrolidone (PVP) progeny Rhizobium - genetics rice seedlings Transformation, Genetic Transgenes transgenic plants |
| title | Agrobacterium-mediated transformation of leaf base derived callus tissues of popular indica rice ( Oryza sativa L. sub sp. indica cv. ADT 43) |
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