The effects of luteolin on osteoclast differentiation, function in vitro and ovariectomy-induced bone loss
Flavonoids, a group of polyphenolic compounds abundant in plants, are known to prevent bone loss in ovariectomized (OVX) animal models. Inhibition of osteoclast differentiation and bone resorption is considered as an effective therapeutic approach in the treatment of postmenopausal bone loss. Luteol...
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| Veröffentlicht in: | The Journal of nutritional biochemistry 2011, Vol.22 (1), p.8-15 |
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| creator | Kim, Tae-Ho Jung, Ji Won Ha, Byung Geun Hong, Jung Min Park, Eui Kyun Kim, Hyun-Ju Kim, Shin-Yoon |
| description | Flavonoids, a group of polyphenolic compounds abundant in plants, are known to prevent bone loss in ovariectomized (OVX) animal models. Inhibition of osteoclast differentiation and bone resorption is considered as an effective therapeutic approach in the treatment of postmenopausal bone loss. Luteolin, a plant flavonoid, has potent anti-inflammatory properties both in vivo and
vitro. In this study, we found that luteolin markedly decreased the differentiation of both bone marrow mononuclear cells and Raw264.7 cells into osteoclasts. Luteolin also inhibited the bone resorptive activity of differentiated osteoclasts. We further investigated the effects of luteolin on ovariectomy-induced bone loss using micro-computed tomography, biomechanical tests and serum markers assay for bone remodeling. Oral administration of luteolin (5 and 20 mg/kg per day) to OVX mice caused significant increase in bone mineral density and bone mineral content of trabecular and cortical bones in the femur as compared to those of OVX controls, and prevented decreases of bone strength indexes induced by OVX surgery. Serum biochemical markers assays revealed that luteolin prevents OVX-induced increases in bone turnover. These data strongly suggest that luteolin has the potential for prevention of bone loss in postmenopausal osteoporosis by reducing both osteoclast differentiation and function. |
| doi_str_mv | 10.1016/j.jnutbio.2009.11.002 |
| format | Article |
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vitro. In this study, we found that luteolin markedly decreased the differentiation of both bone marrow mononuclear cells and Raw264.7 cells into osteoclasts. Luteolin also inhibited the bone resorptive activity of differentiated osteoclasts. We further investigated the effects of luteolin on ovariectomy-induced bone loss using micro-computed tomography, biomechanical tests and serum markers assay for bone remodeling. Oral administration of luteolin (5 and 20 mg/kg per day) to OVX mice caused significant increase in bone mineral density and bone mineral content of trabecular and cortical bones in the femur as compared to those of OVX controls, and prevented decreases of bone strength indexes induced by OVX surgery. Serum biochemical markers assays revealed that luteolin prevents OVX-induced increases in bone turnover. These data strongly suggest that luteolin has the potential for prevention of bone loss in postmenopausal osteoporosis by reducing both osteoclast differentiation and function.</description><identifier>ISSN: 0955-2863</identifier><identifier>EISSN: 1873-4847</identifier><identifier>DOI: 10.1016/j.jnutbio.2009.11.002</identifier><identifier>PMID: 20233653</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Biomarkers - blood ; Biomarkers - metabolism ; Bone and Bones - diagnostic imaging ; Bone and Bones - drug effects ; Bone and Bones - metabolism ; Bone Density Conservation Agents - administration & dosage ; Bone Density Conservation Agents - pharmacology ; Bone Density Conservation Agents - therapeutic use ; Bone loss ; Bone Marrow Cells - cytology ; Bone Marrow Cells - drug effects ; Bone resorption ; Bone Resorption - prevention & control ; Cell Differentiation - drug effects ; Cell Line, Transformed ; Cells, Cultured ; Dose-Response Relationship, Drug ; Feeding. Feeding behavior ; Female ; Flavonoid ; Fundamental and applied biological sciences. Psychology ; Humans ; Leukocytes, Mononuclear - cytology ; Leukocytes, Mononuclear - drug effects ; Luteolin ; Luteolin - administration & dosage ; Luteolin - pharmacology ; Luteolin - therapeutic use ; Macrophages - cytology ; Macrophages - drug effects ; Mice ; Mice, Inbred C57BL ; Mice, Inbred ICR ; Osteoclast ; Osteoclasts - cytology ; Osteoclasts - drug effects ; Osteoclasts - physiology ; Osteoporosis, Postmenopausal - blood ; Osteoporosis, Postmenopausal - diagnostic imaging ; Osteoporosis, Postmenopausal - metabolism ; Osteoporosis, Postmenopausal - prevention & control ; Ovariectomy ; Radiography ; Random Allocation ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>The Journal of nutritional biochemistry, 2011, Vol.22 (1), p.8-15</ispartof><rights>2011 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-34a3d7c0e215b122aa83ff9d48173f323701f7745a52be3fafd1ca414d7fee1b3</citedby><cites>FETCH-LOGICAL-c516t-34a3d7c0e215b122aa83ff9d48173f323701f7745a52be3fafd1ca414d7fee1b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0955286309002447$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23784763$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20233653$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Tae-Ho</creatorcontrib><creatorcontrib>Jung, Ji Won</creatorcontrib><creatorcontrib>Ha, Byung Geun</creatorcontrib><creatorcontrib>Hong, Jung Min</creatorcontrib><creatorcontrib>Park, Eui Kyun</creatorcontrib><creatorcontrib>Kim, Hyun-Ju</creatorcontrib><creatorcontrib>Kim, Shin-Yoon</creatorcontrib><title>The effects of luteolin on osteoclast differentiation, function in vitro and ovariectomy-induced bone loss</title><title>The Journal of nutritional biochemistry</title><addtitle>J Nutr Biochem</addtitle><description>Flavonoids, a group of polyphenolic compounds abundant in plants, are known to prevent bone loss in ovariectomized (OVX) animal models. Inhibition of osteoclast differentiation and bone resorption is considered as an effective therapeutic approach in the treatment of postmenopausal bone loss. Luteolin, a plant flavonoid, has potent anti-inflammatory properties both in vivo and
vitro. In this study, we found that luteolin markedly decreased the differentiation of both bone marrow mononuclear cells and Raw264.7 cells into osteoclasts. Luteolin also inhibited the bone resorptive activity of differentiated osteoclasts. We further investigated the effects of luteolin on ovariectomy-induced bone loss using micro-computed tomography, biomechanical tests and serum markers assay for bone remodeling. Oral administration of luteolin (5 and 20 mg/kg per day) to OVX mice caused significant increase in bone mineral density and bone mineral content of trabecular and cortical bones in the femur as compared to those of OVX controls, and prevented decreases of bone strength indexes induced by OVX surgery. Serum biochemical markers assays revealed that luteolin prevents OVX-induced increases in bone turnover. These data strongly suggest that luteolin has the potential for prevention of bone loss in postmenopausal osteoporosis by reducing both osteoclast differentiation and function.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - blood</subject><subject>Biomarkers - metabolism</subject><subject>Bone and Bones - diagnostic imaging</subject><subject>Bone and Bones - drug effects</subject><subject>Bone and Bones - metabolism</subject><subject>Bone Density Conservation Agents - administration & dosage</subject><subject>Bone Density Conservation Agents - pharmacology</subject><subject>Bone Density Conservation Agents - therapeutic use</subject><subject>Bone loss</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - drug effects</subject><subject>Bone resorption</subject><subject>Bone Resorption - prevention & control</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line, Transformed</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Drug</subject><subject>Feeding. Feeding behavior</subject><subject>Female</subject><subject>Flavonoid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - cytology</subject><subject>Leukocytes, Mononuclear - drug effects</subject><subject>Luteolin</subject><subject>Luteolin - administration & dosage</subject><subject>Luteolin - pharmacology</subject><subject>Luteolin - therapeutic use</subject><subject>Macrophages - cytology</subject><subject>Macrophages - drug effects</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred ICR</subject><subject>Osteoclast</subject><subject>Osteoclasts - cytology</subject><subject>Osteoclasts - drug effects</subject><subject>Osteoclasts - physiology</subject><subject>Osteoporosis, Postmenopausal - blood</subject><subject>Osteoporosis, Postmenopausal - diagnostic imaging</subject><subject>Osteoporosis, Postmenopausal - metabolism</subject><subject>Osteoporosis, Postmenopausal - prevention & control</subject><subject>Ovariectomy</subject><subject>Radiography</subject><subject>Random Allocation</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0955-2863</issn><issn>1873-4847</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctuFDEQRS0EIpPAJwDeoGzoxuVHP1YRinhJkViQrC23XQaPeuxgd4-Uv8fDDLCMZMm1OFW-rkPIK2AtMOjeb9ttXJcppJYzNrYALWP8CdnA0ItGDrJ_SjZsVKrhQyfOyHkpW1YJqbrn5IwzLkSnxIZsb38iRe_RLoUmT-d1wTSHSFM9pdZ2NmWhLlQkY1yCWUKK76hfoz1UtKL7sORETXQ07U0OdVTaPTQhutWio1OKSOdUygvyzJu54MvTfUHuPn28vf7S3Hz7_PX6w01jFXRLI6QRrrcMOagJODdmEN6PTg7QCy-46Bn4vpfKKD6h8MY7sEaCdL1HhElckMvj3Pucfq1YFr0LxeI8m4hpLXoYR5BKyfFxskYYB9bLSqojaXP9SUav73PYmfyggemDD73VJx_64EMD6Lrt2vf69MI67dD96_oroAJvT4Ap1sw-m2hD-c-JvqrsDtybI-dN0uZHrszdd85AMBg5G_5EvDoSWHe7D5h1sQFjVRByVaJdCo-E_Q3ifbZa</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Kim, Tae-Ho</creator><creator>Jung, Ji Won</creator><creator>Ha, Byung Geun</creator><creator>Hong, Jung Min</creator><creator>Park, Eui Kyun</creator><creator>Kim, Hyun-Ju</creator><creator>Kim, Shin-Yoon</creator><general>Elsevier Inc</general><general>New York, NY: Elsevier Science</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QP</scope></search><sort><creationdate>2011</creationdate><title>The effects of luteolin on osteoclast differentiation, function in vitro and ovariectomy-induced bone loss</title><author>Kim, Tae-Ho ; Jung, Ji Won ; Ha, Byung Geun ; Hong, Jung Min ; Park, Eui Kyun ; Kim, Hyun-Ju ; Kim, Shin-Yoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-34a3d7c0e215b122aa83ff9d48173f323701f7745a52be3fafd1ca414d7fee1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - blood</topic><topic>Biomarkers - metabolism</topic><topic>Bone and Bones - diagnostic imaging</topic><topic>Bone and Bones - drug effects</topic><topic>Bone and Bones - metabolism</topic><topic>Bone Density Conservation Agents - administration & dosage</topic><topic>Bone Density Conservation Agents - pharmacology</topic><topic>Bone Density Conservation Agents - therapeutic use</topic><topic>Bone loss</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - drug effects</topic><topic>Bone resorption</topic><topic>Bone Resorption - prevention & control</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line, Transformed</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Drug</topic><topic>Feeding. Feeding behavior</topic><topic>Female</topic><topic>Flavonoid</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Leukocytes, Mononuclear - cytology</topic><topic>Leukocytes, Mononuclear - drug effects</topic><topic>Luteolin</topic><topic>Luteolin - administration & dosage</topic><topic>Luteolin - pharmacology</topic><topic>Luteolin - therapeutic use</topic><topic>Macrophages - cytology</topic><topic>Macrophages - drug effects</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred ICR</topic><topic>Osteoclast</topic><topic>Osteoclasts - cytology</topic><topic>Osteoclasts - drug effects</topic><topic>Osteoclasts - physiology</topic><topic>Osteoporosis, Postmenopausal - blood</topic><topic>Osteoporosis, Postmenopausal - diagnostic imaging</topic><topic>Osteoporosis, Postmenopausal - metabolism</topic><topic>Osteoporosis, Postmenopausal - prevention & control</topic><topic>Ovariectomy</topic><topic>Radiography</topic><topic>Random Allocation</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Tae-Ho</creatorcontrib><creatorcontrib>Jung, Ji Won</creatorcontrib><creatorcontrib>Ha, Byung Geun</creatorcontrib><creatorcontrib>Hong, Jung Min</creatorcontrib><creatorcontrib>Park, Eui Kyun</creatorcontrib><creatorcontrib>Kim, Hyun-Ju</creatorcontrib><creatorcontrib>Kim, Shin-Yoon</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>The Journal of nutritional biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Tae-Ho</au><au>Jung, Ji Won</au><au>Ha, Byung Geun</au><au>Hong, Jung Min</au><au>Park, Eui Kyun</au><au>Kim, Hyun-Ju</au><au>Kim, Shin-Yoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of luteolin on osteoclast differentiation, function in vitro and ovariectomy-induced bone loss</atitle><jtitle>The Journal of nutritional biochemistry</jtitle><addtitle>J Nutr Biochem</addtitle><date>2011</date><risdate>2011</risdate><volume>22</volume><issue>1</issue><spage>8</spage><epage>15</epage><pages>8-15</pages><issn>0955-2863</issn><eissn>1873-4847</eissn><abstract>Flavonoids, a group of polyphenolic compounds abundant in plants, are known to prevent bone loss in ovariectomized (OVX) animal models. Inhibition of osteoclast differentiation and bone resorption is considered as an effective therapeutic approach in the treatment of postmenopausal bone loss. Luteolin, a plant flavonoid, has potent anti-inflammatory properties both in vivo and
vitro. In this study, we found that luteolin markedly decreased the differentiation of both bone marrow mononuclear cells and Raw264.7 cells into osteoclasts. Luteolin also inhibited the bone resorptive activity of differentiated osteoclasts. We further investigated the effects of luteolin on ovariectomy-induced bone loss using micro-computed tomography, biomechanical tests and serum markers assay for bone remodeling. Oral administration of luteolin (5 and 20 mg/kg per day) to OVX mice caused significant increase in bone mineral density and bone mineral content of trabecular and cortical bones in the femur as compared to those of OVX controls, and prevented decreases of bone strength indexes induced by OVX surgery. Serum biochemical markers assays revealed that luteolin prevents OVX-induced increases in bone turnover. These data strongly suggest that luteolin has the potential for prevention of bone loss in postmenopausal osteoporosis by reducing both osteoclast differentiation and function.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>20233653</pmid><doi>10.1016/j.jnutbio.2009.11.002</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Biomarkers - blood Biomarkers - metabolism Bone and Bones - diagnostic imaging Bone and Bones - drug effects Bone and Bones - metabolism Bone Density Conservation Agents - administration & dosage Bone Density Conservation Agents - pharmacology Bone Density Conservation Agents - therapeutic use Bone loss Bone Marrow Cells - cytology Bone Marrow Cells - drug effects Bone resorption Bone Resorption - prevention & control Cell Differentiation - drug effects Cell Line, Transformed Cells, Cultured Dose-Response Relationship, Drug Feeding. Feeding behavior Female Flavonoid Fundamental and applied biological sciences. Psychology Humans Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - drug effects Luteolin Luteolin - administration & dosage Luteolin - pharmacology Luteolin - therapeutic use Macrophages - cytology Macrophages - drug effects Mice Mice, Inbred C57BL Mice, Inbred ICR Osteoclast Osteoclasts - cytology Osteoclasts - drug effects Osteoclasts - physiology Osteoporosis, Postmenopausal - blood Osteoporosis, Postmenopausal - diagnostic imaging Osteoporosis, Postmenopausal - metabolism Osteoporosis, Postmenopausal - prevention & control Ovariectomy Radiography Random Allocation Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | The effects of luteolin on osteoclast differentiation, function in vitro and ovariectomy-induced bone loss |
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