Cloning, sequencing and expression of full-length Campylobacter invasion antigen B gene operon from Campylobacter lari

A novel PCR primer pair for amplification of full‐length cia B gene from thermophilic campylobacters, generated an amplicon of approximately 2.2 kilo base pairs (kbp) with all 18 isolates (n = 7 for urease‐negative (UN) C. lari; n = 9 urease‐positive thermophilic Campylobacter (UPTC); n = 1 C. jejun...

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Veröffentlicht in:	Journal of basic microbiology 2009-08, Vol.49 (4), p.342-349
Hauptverfasser:	Onozato, Junnosuke, Kumagai, Atsuko, Sekizuka, Tsuyoshi, Tazumi, Akihiro, Moore, John E., Millar, B. Cherie, Matsuda, Motoo
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Antigens, Bacterial - genetics Antigens, Bacterial - metabolism Base pairs Base Sequence Campylobacter lari Campylobacter lari - genetics Campylobacter lari - metabolism cia B operon Cloning and sequencing Cloning, Molecular Codons Conserved sequence DNA Primers DNA, Bacterial - genetics Molecular Sequence Data Novel PCR primers Nucleotide sequence Open Reading Frames Operon Operons Phylogeny Polymerase chain reaction Primers Promoter Regions, Genetic Promoters Ribosomes Sequence Alignment Sequence Analysis, DNA Transcription Trees
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