Case report of clinical salmonellosis by Salmonella Typhimurium that occurred in Portuguese children
Aims: Aim of this study is to characterize clinical isolates of Salmonella Typhimurium that occurred in Portuguese children on the basis of their virulence and antimicrobial resistance profiles and pulsed‐field gel electrophoresis typing and to analyse possible strain relatedness. Methods and Result...
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| Veröffentlicht in: | Letters in applied microbiology 2011-09, Vol.53 (3), p.300-305 |
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| description | Aims: Aim of this study is to characterize clinical isolates of Salmonella Typhimurium that occurred in Portuguese children on the basis of their virulence and antimicrobial resistance profiles and pulsed‐field gel electrophoresis typing and to analyse possible strain relatedness. Methods and Results: Different Salmonella serotypes were isolated from clinical cases of salmonellosis that had occurred in two Portuguese hospitals (a total of 259 isolates). All Salm. Typhimurium strains, with the age of the patients known, (total of 26 isolates) were selected for this study. These isolates were characterized for their virulence gene profiles (agfA, iroB, slyA, hin/H2, spv), antimicrobial resistance profiles and investigated for the occurrence of multidrug‐resistant Salm. Typhimurium DT 104 by PCR. Salmonella isolates showed high rates of resistance to four or more antibiotics, 100% resistance to sulfadiazine and a high percentage of strains with the resistance profile of Salm. Typhimurium DT 104, two of them with this phage type (determined by PCR). A relationship between some clusters and their resistance and virulence profiles was detected, each cluster having the same profile. Conclusions: This study showed high‐antibiotic resistance of the Salmonella strains investigated, and the presence of multidrug‐resistant Salm. Typhimurium DT104 in infections of Portuguese children. Significance and Impact of the Study: Study is based on regarding the increase in antibiotic resistance by Salmonella strains isolated from infections in Portuguese children and on the presence of Salm. Typhimurium DT 104 circulating in Portugal. |
| doi_str_mv | 10.1111/j.1472-765X.2011.03106.x |
| format | Article |
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Methods and Results: Different Salmonella serotypes were isolated from clinical cases of salmonellosis that had occurred in two Portuguese hospitals (a total of 259 isolates). All Salm. Typhimurium strains, with the age of the patients known, (total of 26 isolates) were selected for this study. These isolates were characterized for their virulence gene profiles (agfA, iroB, slyA, hin/H2, spv), antimicrobial resistance profiles and investigated for the occurrence of multidrug‐resistant Salm. Typhimurium DT 104 by PCR. Salmonella isolates showed high rates of resistance to four or more antibiotics, 100% resistance to sulfadiazine and a high percentage of strains with the resistance profile of Salm. Typhimurium DT 104, two of them with this phage type (determined by PCR). A relationship between some clusters and their resistance and virulence profiles was detected, each cluster having the same profile. Conclusions: This study showed high‐antibiotic resistance of the Salmonella strains investigated, and the presence of multidrug‐resistant Salm. Typhimurium DT104 in infections of Portuguese children. Significance and Impact of the Study: Study is based on regarding the increase in antibiotic resistance by Salmonella strains isolated from infections in Portuguese children and on the presence of Salm. Typhimurium DT 104 circulating in Portugal.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>DOI: 10.1111/j.1472-765X.2011.03106.x</identifier><identifier>PMID: 21699554</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adolescent ; antibiotic resistance ; antibiotics ; Antimicrobial resistance ; Bacteriophage Typing ; bacteriophages ; Biological and medical sciences ; case studies ; Child ; Child, Preschool ; Children ; Drug Resistance, Multiple, Bacterial - drug effects ; Drug Resistance, Multiple, Bacterial - genetics ; Electrophoresis, Gel, Pulsed-Field ; Female ; Fundamental and applied biological sciences. Psychology ; genes ; hospitals ; Humans ; Infant ; Male ; Microbial Sensitivity Tests ; Microbiology ; multiple drug resistance ; patients ; Polymerase Chain Reaction ; Portugal ; pulsed-field gel electrophoresis ; Salmonella Infections - microbiology ; Salmonella Typhimurium ; Salmonella typhimurium - classification ; Salmonella typhimurium - drug effects ; Salmonella typhimurium - genetics ; Salmonella typhimurium - isolation & purification ; salmonellosis ; serotypes ; sulfadiazine ; virulence</subject><ispartof>Letters in applied microbiology, 2011-09, Vol.53 (3), p.300-305</ispartof><rights>2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology</rights><rights>2015 INIST-CNRS</rights><rights>2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4836-bddc76e0da8b616b14961c523355f8f0cc2bb513f7772459896d9e0c00fe78a3</citedby><orcidid>0000-0003-3977-7418 ; 0000-0002-6296-5137 ; 0000-0002-3663-6604 ; 0000-0002-2798-1924</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1472-765X.2011.03106.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1472-765X.2011.03106.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24427910$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21699554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Freixo, R</creatorcontrib><creatorcontrib>Albano, H</creatorcontrib><creatorcontrib>Silva, J</creatorcontrib><creatorcontrib>Teixeira, P</creatorcontrib><title>Case report of clinical salmonellosis by Salmonella Typhimurium that occurred in Portuguese children</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>Aims: Aim of this study is to characterize clinical isolates of Salmonella Typhimurium that occurred in Portuguese children on the basis of their virulence and antimicrobial resistance profiles and pulsed‐field gel electrophoresis typing and to analyse possible strain relatedness. Methods and Results: Different Salmonella serotypes were isolated from clinical cases of salmonellosis that had occurred in two Portuguese hospitals (a total of 259 isolates). All Salm. Typhimurium strains, with the age of the patients known, (total of 26 isolates) were selected for this study. These isolates were characterized for their virulence gene profiles (agfA, iroB, slyA, hin/H2, spv), antimicrobial resistance profiles and investigated for the occurrence of multidrug‐resistant Salm. Typhimurium DT 104 by PCR. Salmonella isolates showed high rates of resistance to four or more antibiotics, 100% resistance to sulfadiazine and a high percentage of strains with the resistance profile of Salm. Typhimurium DT 104, two of them with this phage type (determined by PCR). A relationship between some clusters and their resistance and virulence profiles was detected, each cluster having the same profile. Conclusions: This study showed high‐antibiotic resistance of the Salmonella strains investigated, and the presence of multidrug‐resistant Salm. Typhimurium DT104 in infections of Portuguese children. Significance and Impact of the Study: Study is based on regarding the increase in antibiotic resistance by Salmonella strains isolated from infections in Portuguese children and on the presence of Salm. Typhimurium DT 104 circulating in Portugal.</description><subject>Adolescent</subject><subject>antibiotic resistance</subject><subject>antibiotics</subject><subject>Antimicrobial resistance</subject><subject>Bacteriophage Typing</subject><subject>bacteriophages</subject><subject>Biological and medical sciences</subject><subject>case studies</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Children</subject><subject>Drug Resistance, Multiple, Bacterial - drug effects</subject><subject>Drug Resistance, Multiple, Bacterial - genetics</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>hospitals</subject><subject>Humans</subject><subject>Infant</subject><subject>Male</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>multiple drug resistance</subject><subject>patients</subject><subject>Polymerase Chain Reaction</subject><subject>Portugal</subject><subject>pulsed-field gel electrophoresis</subject><subject>Salmonella Infections - microbiology</subject><subject>Salmonella Typhimurium</subject><subject>Salmonella typhimurium - classification</subject><subject>Salmonella typhimurium - drug effects</subject><subject>Salmonella typhimurium - genetics</subject><subject>Salmonella typhimurium - isolation & purification</subject><subject>salmonellosis</subject><subject>serotypes</subject><subject>sulfadiazine</subject><subject>virulence</subject><issn>0266-8254</issn><issn>1472-765X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhYMoTjv6FzQgg6sqb56VLFwMjS9oUZgW3IVUKjWdpl4mXTr9703ZPePSbBJyv3s4nIMQJlCSfN7uS8IrWlRS_CgpEFICIyDLu0do9TB4jFZApSwUFfwCPUtpDwCKUP0UXVAitRaCr1Cztsnj6KcxHvDYYteFITjb4WS7fhx8140pJFwf8c39h8Xb47QL_RzD3OPDzuZF5-YYfYPDgL9lpfl29lnW7ULXRD88R09a2yX_4nxfou2H99v1p2Lz9ePn9fWmcFwxWdRN4yrpobGqlkTWhGtJnKCMCdGqFpyjdS0Ia6uqolxopWWjPTiA1lfKskv05iQ7xfFnNnAwfUhucTz4cU5GaU2YBkb_TypOKOFCZfLlmZzr3jdmiqG38WjuE8zA1RmwKefWRju4kP5xnNNKE8jcuxP3O3T--DAnYJZGzd4sxZmlOLM0av42au7M5vrL8sr7r0_70Vk7meh_hXSwKQtwyBrcVAIWu69OVGtHY29jdvL9JusxIDlLBoz9AUYYqNU</recordid><startdate>201109</startdate><enddate>201109</enddate><creator>Freixo, R</creator><creator>Albano, H</creator><creator>Silva, J</creator><creator>Teixeira, P</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><general>Blackwell</general><scope>FBQ</scope><scope>RCLKO</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0003-3977-7418</orcidid><orcidid>https://orcid.org/0000-0002-6296-5137</orcidid><orcidid>https://orcid.org/0000-0002-3663-6604</orcidid><orcidid>https://orcid.org/0000-0002-2798-1924</orcidid></search><sort><creationdate>201109</creationdate><title>Case report of clinical salmonellosis by Salmonella Typhimurium that occurred in Portuguese children</title><author>Freixo, R ; Albano, H ; Silva, J ; Teixeira, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4836-bddc76e0da8b616b14961c523355f8f0cc2bb513f7772459896d9e0c00fe78a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adolescent</topic><topic>antibiotic resistance</topic><topic>antibiotics</topic><topic>Antimicrobial resistance</topic><topic>Bacteriophage Typing</topic><topic>bacteriophages</topic><topic>Biological and medical sciences</topic><topic>case studies</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Children</topic><topic>Drug Resistance, Multiple, Bacterial - drug effects</topic><topic>Drug Resistance, Multiple, Bacterial - genetics</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>hospitals</topic><topic>Humans</topic><topic>Infant</topic><topic>Male</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>multiple drug resistance</topic><topic>patients</topic><topic>Polymerase Chain Reaction</topic><topic>Portugal</topic><topic>pulsed-field gel electrophoresis</topic><topic>Salmonella Infections - microbiology</topic><topic>Salmonella Typhimurium</topic><topic>Salmonella typhimurium - classification</topic><topic>Salmonella typhimurium - drug effects</topic><topic>Salmonella typhimurium - genetics</topic><topic>Salmonella typhimurium - isolation & purification</topic><topic>salmonellosis</topic><topic>serotypes</topic><topic>sulfadiazine</topic><topic>virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Freixo, R</creatorcontrib><creatorcontrib>Albano, H</creatorcontrib><creatorcontrib>Silva, J</creatorcontrib><creatorcontrib>Teixeira, P</creatorcontrib><collection>AGRIS</collection><collection>RCAAP open access repository</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Freixo, R</au><au>Albano, H</au><au>Silva, J</au><au>Teixeira, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Case report of clinical salmonellosis by Salmonella Typhimurium that occurred in Portuguese children</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2011-09</date><risdate>2011</risdate><volume>53</volume><issue>3</issue><spage>300</spage><epage>305</epage><pages>300-305</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><coden>LAMIE7</coden><abstract>Aims: Aim of this study is to characterize clinical isolates of Salmonella Typhimurium that occurred in Portuguese children on the basis of their virulence and antimicrobial resistance profiles and pulsed‐field gel electrophoresis typing and to analyse possible strain relatedness. Methods and Results: Different Salmonella serotypes were isolated from clinical cases of salmonellosis that had occurred in two Portuguese hospitals (a total of 259 isolates). All Salm. Typhimurium strains, with the age of the patients known, (total of 26 isolates) were selected for this study. These isolates were characterized for their virulence gene profiles (agfA, iroB, slyA, hin/H2, spv), antimicrobial resistance profiles and investigated for the occurrence of multidrug‐resistant Salm. Typhimurium DT 104 by PCR. Salmonella isolates showed high rates of resistance to four or more antibiotics, 100% resistance to sulfadiazine and a high percentage of strains with the resistance profile of Salm. Typhimurium DT 104, two of them with this phage type (determined by PCR). A relationship between some clusters and their resistance and virulence profiles was detected, each cluster having the same profile. Conclusions: This study showed high‐antibiotic resistance of the Salmonella strains investigated, and the presence of multidrug‐resistant Salm. Typhimurium DT104 in infections of Portuguese children. Significance and Impact of the Study: Study is based on regarding the increase in antibiotic resistance by Salmonella strains isolated from infections in Portuguese children and on the presence of Salm. Typhimurium DT 104 circulating in Portugal.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21699554</pmid><doi>10.1111/j.1472-765X.2011.03106.x</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-3977-7418</orcidid><orcidid>https://orcid.org/0000-0002-6296-5137</orcidid><orcidid>https://orcid.org/0000-0002-3663-6604</orcidid><orcidid>https://orcid.org/0000-0002-2798-1924</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent antibiotic resistance antibiotics Antimicrobial resistance Bacteriophage Typing bacteriophages Biological and medical sciences case studies Child Child, Preschool Children Drug Resistance, Multiple, Bacterial - drug effects Drug Resistance, Multiple, Bacterial - genetics Electrophoresis, Gel, Pulsed-Field Female Fundamental and applied biological sciences. Psychology genes hospitals Humans Infant Male Microbial Sensitivity Tests Microbiology multiple drug resistance patients Polymerase Chain Reaction Portugal pulsed-field gel electrophoresis Salmonella Infections - microbiology Salmonella Typhimurium Salmonella typhimurium - classification Salmonella typhimurium - drug effects Salmonella typhimurium - genetics Salmonella typhimurium - isolation & purification salmonellosis serotypes sulfadiazine virulence |
| title | Case report of clinical salmonellosis by Salmonella Typhimurium that occurred in Portuguese children |
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