Identification of Evi-1 as a novel effector of PKCδ in the apoptotic response to DNA damage
Protein kinase C delta (PKCδ), a PKC family isoform, regulates diverse signal transduction pathways during DNA damage to induce apoptosis. To explore the apoptosis mechanism that PKCδ modulates, we sought to uncover transcription factor targets of PKCδ by devising a screening strategy that utilizes...
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| Veröffentlicht in: | Biochimica et biophysica acta 2011-07, Vol.1809 (7), p.285-294 |
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| container_title | Biochimica et biophysica acta |
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| creator | Hew, Hoi Chin Liu, Hanshao Lu, Zheng-Guang Kimura, Junko Miki, Yoshio Yoshida, Kiyotsugu |
| description | Protein kinase C delta (PKCδ), a PKC family isoform, regulates diverse signal transduction pathways during DNA damage to induce apoptosis. To explore the apoptosis mechanism that PKCδ modulates, we sought to uncover transcription factor targets of PKCδ by devising a screening strategy that utilizes ChIP-cloning and microarray analysis. Transcription factor candidates were generated with the application of public access data-mining tools and this resulted in the identification of Evi-1 as a novel PKCδ-mediated DNA damage responsive molecule. The results demonstrated that PKCδ is constitutively associated with Evi-1. PKCδ regulated Evi-1 to activate PLZF transcription upon genotoxic stress. Furthermore, both Evi-1 and PLZF were associated with DNA damage-stimulated apoptosis. Taken together, we have discovered a novel regulation of Evi-1, which transactivates PLZF, by PKCδ to induce cell death in response to genotoxic stress.
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► PKCδ induces apoptosis in response to DNA damage. ► PKCδ regulates Evi-1 to activate PLZF transcription. ► Evi-1 is associated with DNA damage-stimulated cell death. |
| doi_str_mv | 10.1016/j.bbagrm.2011.02.004 |
| format | Article |
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► PKCδ induces apoptosis in response to DNA damage. ► PKCδ regulates Evi-1 to activate PLZF transcription. ► Evi-1 is associated with DNA damage-stimulated cell death.</description><identifier>ISSN: 1874-9399</identifier><identifier>ISSN: 0006-3002</identifier><identifier>EISSN: 1876-4320</identifier><identifier>DOI: 10.1016/j.bbagrm.2011.02.004</identifier><identifier>PMID: 21362509</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Apoptosis ; Apoptosis - physiology ; Cell Line, Tumor ; DNA damage ; DNA Damage - physiology ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Evi-1 ; Humans ; Kruppel-Like Transcription Factors - genetics ; Kruppel-Like Transcription Factors - metabolism ; MDS1 and EVI1 Complex Locus Protein ; PKCδ ; PLZF ; Promyelocytic Leukemia Zinc Finger Protein ; Protein Kinase C-delta - genetics ; Protein Kinase C-delta - metabolism ; Proto-Oncogenes - genetics ; Signal Transduction - physiology ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic - physiology</subject><ispartof>Biochimica et biophysica acta, 2011-07, Vol.1809 (7), p.285-294</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-8b6e05a5dc7620136e0c786c48abd203b2048a7c266f170090902bd2025bba8e3</citedby><cites>FETCH-LOGICAL-c393t-8b6e05a5dc7620136e0c786c48abd203b2048a7c266f170090902bd2025bba8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1874939911000472$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21362509$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hew, Hoi Chin</creatorcontrib><creatorcontrib>Liu, Hanshao</creatorcontrib><creatorcontrib>Lu, Zheng-Guang</creatorcontrib><creatorcontrib>Kimura, Junko</creatorcontrib><creatorcontrib>Miki, Yoshio</creatorcontrib><creatorcontrib>Yoshida, Kiyotsugu</creatorcontrib><title>Identification of Evi-1 as a novel effector of PKCδ in the apoptotic response to DNA damage</title><title>Biochimica et biophysica acta</title><addtitle>Biochim Biophys Acta</addtitle><description>Protein kinase C delta (PKCδ), a PKC family isoform, regulates diverse signal transduction pathways during DNA damage to induce apoptosis. To explore the apoptosis mechanism that PKCδ modulates, we sought to uncover transcription factor targets of PKCδ by devising a screening strategy that utilizes ChIP-cloning and microarray analysis. Transcription factor candidates were generated with the application of public access data-mining tools and this resulted in the identification of Evi-1 as a novel PKCδ-mediated DNA damage responsive molecule. The results demonstrated that PKCδ is constitutively associated with Evi-1. PKCδ regulated Evi-1 to activate PLZF transcription upon genotoxic stress. Furthermore, both Evi-1 and PLZF were associated with DNA damage-stimulated apoptosis. Taken together, we have discovered a novel regulation of Evi-1, which transactivates PLZF, by PKCδ to induce cell death in response to genotoxic stress.
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► PKCδ induces apoptosis in response to DNA damage. ► PKCδ regulates Evi-1 to activate PLZF transcription. ► Evi-1 is associated with DNA damage-stimulated cell death.</description><subject>Apoptosis</subject><subject>Apoptosis - physiology</subject><subject>Cell Line, Tumor</subject><subject>DNA damage</subject><subject>DNA Damage - physiology</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Evi-1</subject><subject>Humans</subject><subject>Kruppel-Like Transcription Factors - genetics</subject><subject>Kruppel-Like Transcription Factors - metabolism</subject><subject>MDS1 and EVI1 Complex Locus Protein</subject><subject>PKCδ</subject><subject>PLZF</subject><subject>Promyelocytic Leukemia Zinc Finger Protein</subject><subject>Protein Kinase C-delta - genetics</subject><subject>Protein Kinase C-delta - metabolism</subject><subject>Proto-Oncogenes - genetics</subject><subject>Signal Transduction - physiology</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic - physiology</subject><issn>1874-9399</issn><issn>0006-3002</issn><issn>1876-4320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9O3DAQxi1EVf71DVDlW08JYzvrOJdKaFkKKioc6K2S5TgT8GoTp7Z3pb4Xz9FnqpcFjkVzmLHmmxn59xFyyqBkwOTZsmxb8xCGkgNjJfASoNojh0zVsqgEh_3nuioa0TQH5CjGJYBkHOAjOeBMSD6D5pD8uu5wTK531iTnR-p7uti4glETqaGj3-CKYt-jTT5sm3ff53-fqBtpekRqJj8ln5ylAePkx4g0eXrx45x2ZjAPeEI-9GYV8dNLPiY_Lxf386vi5vbb9fz8prCiEalQrUSYmVlna5k_I_LL1kraSpm24yBaDrmsLZeyZzVAk4NvO3yWESgUx-TLbu8U_O81xqQHFy2uVmZEv45aNQ0TSoF8X6mgZlXmlpXVTmmDjzFgr6fgBhP-aAZ6a4Be6p0BemuABq6zAXns88uBdTtg9zb0SjwLvu4EmIFsHAYdrcPRYudCpqw77_5_4R-kHZbM</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Hew, Hoi Chin</creator><creator>Liu, Hanshao</creator><creator>Lu, Zheng-Guang</creator><creator>Kimura, Junko</creator><creator>Miki, Yoshio</creator><creator>Yoshida, Kiyotsugu</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20110701</creationdate><title>Identification of Evi-1 as a novel effector of PKCδ in the apoptotic response to DNA damage</title><author>Hew, Hoi Chin ; Liu, Hanshao ; Lu, Zheng-Guang ; Kimura, Junko ; Miki, Yoshio ; Yoshida, Kiyotsugu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-8b6e05a5dc7620136e0c786c48abd203b2048a7c266f170090902bd2025bba8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Apoptosis</topic><topic>Apoptosis - physiology</topic><topic>Cell Line, Tumor</topic><topic>DNA damage</topic><topic>DNA Damage - physiology</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Evi-1</topic><topic>Humans</topic><topic>Kruppel-Like Transcription Factors - genetics</topic><topic>Kruppel-Like Transcription Factors - metabolism</topic><topic>MDS1 and EVI1 Complex Locus Protein</topic><topic>PKCδ</topic><topic>PLZF</topic><topic>Promyelocytic Leukemia Zinc Finger Protein</topic><topic>Protein Kinase C-delta - genetics</topic><topic>Protein Kinase C-delta - metabolism</topic><topic>Proto-Oncogenes - genetics</topic><topic>Signal Transduction - physiology</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hew, Hoi Chin</creatorcontrib><creatorcontrib>Liu, Hanshao</creatorcontrib><creatorcontrib>Lu, Zheng-Guang</creatorcontrib><creatorcontrib>Kimura, Junko</creatorcontrib><creatorcontrib>Miki, Yoshio</creatorcontrib><creatorcontrib>Yoshida, Kiyotsugu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Biochimica et biophysica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hew, Hoi Chin</au><au>Liu, Hanshao</au><au>Lu, Zheng-Guang</au><au>Kimura, Junko</au><au>Miki, Yoshio</au><au>Yoshida, Kiyotsugu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Evi-1 as a novel effector of PKCδ in the apoptotic response to DNA damage</atitle><jtitle>Biochimica et biophysica acta</jtitle><addtitle>Biochim Biophys Acta</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>1809</volume><issue>7</issue><spage>285</spage><epage>294</epage><pages>285-294</pages><issn>1874-9399</issn><issn>0006-3002</issn><eissn>1876-4320</eissn><abstract>Protein kinase C delta (PKCδ), a PKC family isoform, regulates diverse signal transduction pathways during DNA damage to induce apoptosis. To explore the apoptosis mechanism that PKCδ modulates, we sought to uncover transcription factor targets of PKCδ by devising a screening strategy that utilizes ChIP-cloning and microarray analysis. Transcription factor candidates were generated with the application of public access data-mining tools and this resulted in the identification of Evi-1 as a novel PKCδ-mediated DNA damage responsive molecule. The results demonstrated that PKCδ is constitutively associated with Evi-1. PKCδ regulated Evi-1 to activate PLZF transcription upon genotoxic stress. Furthermore, both Evi-1 and PLZF were associated with DNA damage-stimulated apoptosis. Taken together, we have discovered a novel regulation of Evi-1, which transactivates PLZF, by PKCδ to induce cell death in response to genotoxic stress.
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► PKCδ induces apoptosis in response to DNA damage. ► PKCδ regulates Evi-1 to activate PLZF transcription. ► Evi-1 is associated with DNA damage-stimulated cell death.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21362509</pmid><doi>10.1016/j.bbagrm.2011.02.004</doi><tpages>10</tpages></addata></record> |
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| subjects | Apoptosis Apoptosis - physiology Cell Line, Tumor DNA damage DNA Damage - physiology DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Evi-1 Humans Kruppel-Like Transcription Factors - genetics Kruppel-Like Transcription Factors - metabolism MDS1 and EVI1 Complex Locus Protein PKCδ PLZF Promyelocytic Leukemia Zinc Finger Protein Protein Kinase C-delta - genetics Protein Kinase C-delta - metabolism Proto-Oncogenes - genetics Signal Transduction - physiology Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic - physiology |
| title | Identification of Evi-1 as a novel effector of PKCδ in the apoptotic response to DNA damage |
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