Toxicity of Seven Bacillus thuringiensis Cry Proteins Against Cylas puncticollis and Cylas brunneus (Coleoptera: Brentidae) Using a Novel Artificial Diet
“Sweetpotato weevils” Cylas puncticollis (Boheman) and Cylas brunneus F. (Coleoptera: Brentidae) are the most important biological threat to sweetpotato, Ipomoea batatas L. (Lam), productivity in sub-Saharan Africa. Sweetpotato weevil control is difficult due to their cryptic feeding behavior. Expre...
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| Veröffentlicht in: | Journal of economic entomology 2010-08, Vol.103 (4), p.1493-1502 |
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| creator | Ekobu, Moses Solera, Maureen Kyamanywa, Samuel Mwanga, Robert O. M. Odongo, Benson Ghislain, Marc Moar, William J. |
| description | “Sweetpotato weevils” Cylas puncticollis (Boheman) and Cylas brunneus F. (Coleoptera: Brentidae) are the most important biological threat to sweetpotato, Ipomoea batatas L. (Lam), productivity in sub-Saharan Africa. Sweetpotato weevil control is difficult due to their cryptic feeding behavior. Expression of Cylas-active Bacillus thuringiensis (Bt) Cry proteins in sweetpotato could provide an effective control strategy. Unfortunately, Bt Cry proteins with relatively high toxicity against Cylas spp. have not been identified, partly because no published methodology for screening Bt Cry proteins against Cylas spp. in artificial diet exists. Therefore, the initial aim of this study was to develop an artificial diet for conducting bioassays with Cylas spp. and then to determine Bt Cry protein efficacy against C. puncticollis and C. brunneus by using this artificial diet. Five diets varying in their composition were evaluated. The highest survival rates for sweetpotato weevil larvae were observed for diet E that contained the highest amount of sweetpotato powder and supported weevil development from first instar to adulthood, similar to sweetpotato storage roots. Seven coleopteranactive Bt Cry proteins were incorporated into diet E and toxicity data were generated against neonate C.puncticollis and second-instar C.brunneus. All Bt Cry proteins tested had toxicity greater than the untreated control. Cry7Aa1, ET33/34, and Cry3Ca1 had LC50 values below 1 µg/g diet against both species. This study demonstrates the feasibility of using an artificial diet bioassay for screening Bt Cry proteins against sweetpotato weevil larvae and identifies candidate Bt Cry proteins for use in transforming sweetpotato varieties potentially conferring field resistance against these pests. |
| doi_str_mv | 10.1603/EC09432 |
| format | Article |
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M. ; Odongo, Benson ; Ghislain, Marc ; Moar, William J.</creator><creatorcontrib>Ekobu, Moses ; Solera, Maureen ; Kyamanywa, Samuel ; Mwanga, Robert O. M. ; Odongo, Benson ; Ghislain, Marc ; Moar, William J.</creatorcontrib><description>“Sweetpotato weevils” Cylas puncticollis (Boheman) and Cylas brunneus F. (Coleoptera: Brentidae) are the most important biological threat to sweetpotato, Ipomoea batatas L. (Lam), productivity in sub-Saharan Africa. Sweetpotato weevil control is difficult due to their cryptic feeding behavior. Expression of Cylas-active Bacillus thuringiensis (Bt) Cry proteins in sweetpotato could provide an effective control strategy. Unfortunately, Bt Cry proteins with relatively high toxicity against Cylas spp. have not been identified, partly because no published methodology for screening Bt Cry proteins against Cylas spp. in artificial diet exists. Therefore, the initial aim of this study was to develop an artificial diet for conducting bioassays with Cylas spp. and then to determine Bt Cry protein efficacy against C. puncticollis and C. brunneus by using this artificial diet. Five diets varying in their composition were evaluated. The highest survival rates for sweetpotato weevil larvae were observed for diet E that contained the highest amount of sweetpotato powder and supported weevil development from first instar to adulthood, similar to sweetpotato storage roots. Seven coleopteranactive Bt Cry proteins were incorporated into diet E and toxicity data were generated against neonate C.puncticollis and second-instar C.brunneus. All Bt Cry proteins tested had toxicity greater than the untreated control. Cry7Aa1, ET33/34, and Cry3Ca1 had LC50 values below 1 µg/g diet against both species. This study demonstrates the feasibility of using an artificial diet bioassay for screening Bt Cry proteins against sweetpotato weevil larvae and identifies candidate Bt Cry proteins for use in transforming sweetpotato varieties potentially conferring field resistance against these pests.</description><identifier>ISSN: 0022-0493</identifier><identifier>EISSN: 1938-291X</identifier><identifier>EISSN: 0022-0493</identifier><identifier>DOI: 10.1603/EC09432</identifier><identifier>PMID: 20857765</identifier><identifier>CODEN: JEENAI</identifier><language>eng</language><publisher>Lanham, MD: Entomological Society of America</publisher><subject>Animal, plant and microbial ecology ; Animals ; Applied ecology ; Artificial diets ; Bacillus thuringiensis ; Bacterial Proteins - classification ; Bacterial Proteins - pharmacology ; Biological and medical sciences ; Brentidae ; Bt bioassays ; Bt toxins ; Coleoptera ; Coleoptera - drug effects ; Control ; CRY protein ; crystal proteins ; Cylas ; Cylas brunneus ; Cylas puncticollis ; Data processing ; Diet ; Diets ; Ecotoxicology, biological effects of pollution ; Endotoxins - classification ; Endotoxins - pharmacology ; Feeding behavior ; Fundamental and applied biological sciences. Psychology ; gene expression ; Generalities ; Hemolysin Proteins - classification ; Hemolysin Proteins - pharmacology ; insect control ; insect pests ; insect rearing ; Insecta ; insectaries ; insecticidal proteins ; Insecticides - classification ; Insecticides - pharmacology ; Invertebrates ; Ipomoea batatas ; larvae ; lethal dose 50 ; Neonates ; new methods ; optimization ; Phytopathology. Animal pests. Plant and forest protection ; plant pests ; Powder ; powders ; Protozoa. Invertebrates ; Roots ; screening ; STORED-PRODUCT ; Survival ; sweet potatoes ; Toxicity ; toxicity testing ; transgenic plants</subject><ispartof>Journal of economic entomology, 2010-08, Vol.103 (4), p.1493-1502</ispartof><rights>2010 Entomological Society of America</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b565t-4157f70a13dfad2138f251a422c2d7f5089b4027b2d2c2d82501e57a28f21c8e3</citedby><cites>FETCH-LOGICAL-b565t-4157f70a13dfad2138f251a422c2d7f5089b4027b2d2c2d82501e57a28f21c8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1603/EC09432$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>314,780,784,26978,27924,27925,52363</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23083685$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20857765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ekobu, Moses</creatorcontrib><creatorcontrib>Solera, Maureen</creatorcontrib><creatorcontrib>Kyamanywa, Samuel</creatorcontrib><creatorcontrib>Mwanga, Robert O. M.</creatorcontrib><creatorcontrib>Odongo, Benson</creatorcontrib><creatorcontrib>Ghislain, Marc</creatorcontrib><creatorcontrib>Moar, William J.</creatorcontrib><title>Toxicity of Seven Bacillus thuringiensis Cry Proteins Against Cylas puncticollis and Cylas brunneus (Coleoptera: Brentidae) Using a Novel Artificial Diet</title><title>Journal of economic entomology</title><addtitle>J Econ Entomol</addtitle><description>“Sweetpotato weevils” Cylas puncticollis (Boheman) and Cylas brunneus F. (Coleoptera: Brentidae) are the most important biological threat to sweetpotato, Ipomoea batatas L. (Lam), productivity in sub-Saharan Africa. Sweetpotato weevil control is difficult due to their cryptic feeding behavior. Expression of Cylas-active Bacillus thuringiensis (Bt) Cry proteins in sweetpotato could provide an effective control strategy. Unfortunately, Bt Cry proteins with relatively high toxicity against Cylas spp. have not been identified, partly because no published methodology for screening Bt Cry proteins against Cylas spp. in artificial diet exists. Therefore, the initial aim of this study was to develop an artificial diet for conducting bioassays with Cylas spp. and then to determine Bt Cry protein efficacy against C. puncticollis and C. brunneus by using this artificial diet. Five diets varying in their composition were evaluated. The highest survival rates for sweetpotato weevil larvae were observed for diet E that contained the highest amount of sweetpotato powder and supported weevil development from first instar to adulthood, similar to sweetpotato storage roots. Seven coleopteranactive Bt Cry proteins were incorporated into diet E and toxicity data were generated against neonate C.puncticollis and second-instar C.brunneus. All Bt Cry proteins tested had toxicity greater than the untreated control. Cry7Aa1, ET33/34, and Cry3Ca1 had LC50 values below 1 µg/g diet against both species. This study demonstrates the feasibility of using an artificial diet bioassay for screening Bt Cry proteins against sweetpotato weevil larvae and identifies candidate Bt Cry proteins for use in transforming sweetpotato varieties potentially conferring field resistance against these pests.</description><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>Artificial diets</subject><subject>Bacillus thuringiensis</subject><subject>Bacterial Proteins - classification</subject><subject>Bacterial Proteins - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Brentidae</subject><subject>Bt bioassays</subject><subject>Bt toxins</subject><subject>Coleoptera</subject><subject>Coleoptera - drug effects</subject><subject>Control</subject><subject>CRY protein</subject><subject>crystal proteins</subject><subject>Cylas</subject><subject>Cylas brunneus</subject><subject>Cylas puncticollis</subject><subject>Data processing</subject><subject>Diet</subject><subject>Diets</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Endotoxins - classification</subject><subject>Endotoxins - pharmacology</subject><subject>Feeding behavior</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Generalities</subject><subject>Hemolysin Proteins - classification</subject><subject>Hemolysin Proteins - pharmacology</subject><subject>insect control</subject><subject>insect pests</subject><subject>insect rearing</subject><subject>Insecta</subject><subject>insectaries</subject><subject>insecticidal proteins</subject><subject>Insecticides - classification</subject><subject>Insecticides - pharmacology</subject><subject>Invertebrates</subject><subject>Ipomoea batatas</subject><subject>larvae</subject><subject>lethal dose 50</subject><subject>Neonates</subject><subject>new methods</subject><subject>optimization</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>plant pests</subject><subject>Powder</subject><subject>powders</subject><subject>Protozoa. Invertebrates</subject><subject>Roots</subject><subject>screening</subject><subject>STORED-PRODUCT</subject><subject>Survival</subject><subject>sweet potatoes</subject><subject>Toxicity</subject><subject>toxicity testing</subject><subject>transgenic plants</subject><issn>0022-0493</issn><issn>1938-291X</issn><issn>0022-0493</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0d1qFDEUwPEgil2r-AaaG7FejOZjskm8207rBxQV2gXvhkwmWSPZZE0yxX0U39YsHa030qsDhx9_DhwAnmL0Gi8RfXPeIdlScg8ssKSiIRJ_vQ8WCBHSoFbSI_Ao5-8I4SXB6CE4IkgwzpdsAX5dxZ9Ou7KH0cJLc20CPFXaeT9lWL5NyYWNMyG7DLu0h19SLMaFDFcbVUeB3d6rDHdT0MXp6H11KozzekhTCKaGTrroTdwVk9RbeJpMKG5U5hVc55qHCn6K18bDVSrO1luUh2fOlMfggVU-myfzPAbrd-dX3Yfm4vP7j93qohnYkpWmxYxbjhSmo1UjwVRYwrBqCdFk5JYhIYcWET6Q8bARhCFsGFekOqyFocfg5U13l-KPyeTSb13WxnsVTJxyL6TElOJavlMy2nIhKLlTcsaw5PLfpk4x52Rsv0tuq9K-x6g_vLafX1vls7k5DVsz_nV_flnBixmorJW3SQXt8q2jSNClOLjnN86q2KtNqmZ9SRCmCAvOkZS3pcHFGMx_T_oNxaq__A</recordid><startdate>20100801</startdate><enddate>20100801</enddate><creator>Ekobu, Moses</creator><creator>Solera, Maureen</creator><creator>Kyamanywa, Samuel</creator><creator>Mwanga, Robert O. 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Psychology</topic><topic>gene expression</topic><topic>Generalities</topic><topic>Hemolysin Proteins - classification</topic><topic>Hemolysin Proteins - pharmacology</topic><topic>insect control</topic><topic>insect pests</topic><topic>insect rearing</topic><topic>Insecta</topic><topic>insectaries</topic><topic>insecticidal proteins</topic><topic>Insecticides - classification</topic><topic>Insecticides - pharmacology</topic><topic>Invertebrates</topic><topic>Ipomoea batatas</topic><topic>larvae</topic><topic>lethal dose 50</topic><topic>Neonates</topic><topic>new methods</topic><topic>optimization</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>plant pests</topic><topic>Powder</topic><topic>powders</topic><topic>Protozoa. Invertebrates</topic><topic>Roots</topic><topic>screening</topic><topic>STORED-PRODUCT</topic><topic>Survival</topic><topic>sweet potatoes</topic><topic>Toxicity</topic><topic>toxicity testing</topic><topic>transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ekobu, Moses</creatorcontrib><creatorcontrib>Solera, Maureen</creatorcontrib><creatorcontrib>Kyamanywa, Samuel</creatorcontrib><creatorcontrib>Mwanga, Robert O. 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M.</au><au>Odongo, Benson</au><au>Ghislain, Marc</au><au>Moar, William J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Toxicity of Seven Bacillus thuringiensis Cry Proteins Against Cylas puncticollis and Cylas brunneus (Coleoptera: Brentidae) Using a Novel Artificial Diet</atitle><jtitle>Journal of economic entomology</jtitle><addtitle>J Econ Entomol</addtitle><date>2010-08-01</date><risdate>2010</risdate><volume>103</volume><issue>4</issue><spage>1493</spage><epage>1502</epage><pages>1493-1502</pages><issn>0022-0493</issn><eissn>1938-291X</eissn><eissn>0022-0493</eissn><coden>JEENAI</coden><abstract>“Sweetpotato weevils” Cylas puncticollis (Boheman) and Cylas brunneus F. (Coleoptera: Brentidae) are the most important biological threat to sweetpotato, Ipomoea batatas L. (Lam), productivity in sub-Saharan Africa. Sweetpotato weevil control is difficult due to their cryptic feeding behavior. Expression of Cylas-active Bacillus thuringiensis (Bt) Cry proteins in sweetpotato could provide an effective control strategy. Unfortunately, Bt Cry proteins with relatively high toxicity against Cylas spp. have not been identified, partly because no published methodology for screening Bt Cry proteins against Cylas spp. in artificial diet exists. Therefore, the initial aim of this study was to develop an artificial diet for conducting bioassays with Cylas spp. and then to determine Bt Cry protein efficacy against C. puncticollis and C. brunneus by using this artificial diet. Five diets varying in their composition were evaluated. The highest survival rates for sweetpotato weevil larvae were observed for diet E that contained the highest amount of sweetpotato powder and supported weevil development from first instar to adulthood, similar to sweetpotato storage roots. Seven coleopteranactive Bt Cry proteins were incorporated into diet E and toxicity data were generated against neonate C.puncticollis and second-instar C.brunneus. All Bt Cry proteins tested had toxicity greater than the untreated control. Cry7Aa1, ET33/34, and Cry3Ca1 had LC50 values below 1 µg/g diet against both species. This study demonstrates the feasibility of using an artificial diet bioassay for screening Bt Cry proteins against sweetpotato weevil larvae and identifies candidate Bt Cry proteins for use in transforming sweetpotato varieties potentially conferring field resistance against these pests.</abstract><cop>Lanham, MD</cop><pub>Entomological Society of America</pub><pmid>20857765</pmid><doi>10.1603/EC09432</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of economic entomology, 2010-08, Vol.103 (4), p.1493-1502 |
| issn | 0022-0493 1938-291X 0022-0493 |
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| subjects | Animal, plant and microbial ecology Animals Applied ecology Artificial diets Bacillus thuringiensis Bacterial Proteins - classification Bacterial Proteins - pharmacology Biological and medical sciences Brentidae Bt bioassays Bt toxins Coleoptera Coleoptera - drug effects Control CRY protein crystal proteins Cylas Cylas brunneus Cylas puncticollis Data processing Diet Diets Ecotoxicology, biological effects of pollution Endotoxins - classification Endotoxins - pharmacology Feeding behavior Fundamental and applied biological sciences. Psychology gene expression Generalities Hemolysin Proteins - classification Hemolysin Proteins - pharmacology insect control insect pests insect rearing Insecta insectaries insecticidal proteins Insecticides - classification Insecticides - pharmacology Invertebrates Ipomoea batatas larvae lethal dose 50 Neonates new methods optimization Phytopathology. Animal pests. Plant and forest protection plant pests Powder powders Protozoa. Invertebrates Roots screening STORED-PRODUCT Survival sweet potatoes Toxicity toxicity testing transgenic plants |
| title | Toxicity of Seven Bacillus thuringiensis Cry Proteins Against Cylas puncticollis and Cylas brunneus (Coleoptera: Brentidae) Using a Novel Artificial Diet |
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