Mycobacterial cell-wall skeleton as a universal vaccine vehicle for antigen conjugation
Abstracts Mycobacterial cell-wall skeleton (CWS) is an immunoactive and biodegradable particulate adjuvant and has been used for immunotherapy in patients with cancer. The CWS of Mycobacterium bovis bacillus Calmette-Guérin (BCG-CWS) was studied as a universal vaccine vehicle for antigen conjugation...
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| Veröffentlicht in: | Vaccine 2010-11, Vol.28 (50), p.7873-7880 |
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| creator | Paik, Tae-Hyun Lee, Ji-Sook Kim, Ki-Hye Yang, Chul-Su Jo, Eun-Kyeong Song, Chang-Hwa |
| description | Abstracts Mycobacterial cell-wall skeleton (CWS) is an immunoactive and biodegradable particulate adjuvant and has been used for immunotherapy in patients with cancer. The CWS of Mycobacterium bovis bacillus Calmette-Guérin (BCG-CWS) was studied as a universal vaccine vehicle for antigen conjugation, to develop potentially effective and safe vaccines. Here, we describe experiments in which protein antigens, such as keyhole limpet haemocyanin (KLH), ovalbumin (OVA) and bovine serum albumin (BSA) were highly efficiently coupled to 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N -hydroxysuccinimide (EDC/NHS)-activated carboxyl groups of BCG-CWS, and tested the immunogenicity of OVA-conjugated BCG-CWS vaccine. We found that a strong immune response was induced in mice immunised with OVA-conjugated BCG-CWS, which was similar to the enhancement of the immune responses in mice immunised with OVA and complete Freund's adjuvant. Covalent conjugation of OVA to BCG-CWS was essential for Th1-skewed immune responses, with prominent expression of IFN-γ. Furthermore, antigen-conjugated BCG-CWS vaccine is simple to manufacture, safe, and easy to use. Our results suggest that mycobacterial CWS as a universal vaccine vehicle for conjugation of a wide variety of antigens constitutes a breakthrough for development of the most promising vaccines for infections, allergic diseases, and cancer. |
| doi_str_mv | 10.1016/j.vaccine.2010.09.083 |
| format | Article |
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The CWS of Mycobacterium bovis bacillus Calmette-Guérin (BCG-CWS) was studied as a universal vaccine vehicle for antigen conjugation, to develop potentially effective and safe vaccines. Here, we describe experiments in which protein antigens, such as keyhole limpet haemocyanin (KLH), ovalbumin (OVA) and bovine serum albumin (BSA) were highly efficiently coupled to 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N -hydroxysuccinimide (EDC/NHS)-activated carboxyl groups of BCG-CWS, and tested the immunogenicity of OVA-conjugated BCG-CWS vaccine. We found that a strong immune response was induced in mice immunised with OVA-conjugated BCG-CWS, which was similar to the enhancement of the immune responses in mice immunised with OVA and complete Freund's adjuvant. Covalent conjugation of OVA to BCG-CWS was essential for Th1-skewed immune responses, with prominent expression of IFN-γ. Furthermore, antigen-conjugated BCG-CWS vaccine is simple to manufacture, safe, and easy to use. Our results suggest that mycobacterial CWS as a universal vaccine vehicle for conjugation of a wide variety of antigens constitutes a breakthrough for development of the most promising vaccines for infections, allergic diseases, and cancer.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2010.09.083</identifier><identifier>PMID: 20937311</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Adjuvant ; Adjuvants, Immunologic - pharmacology ; Allergy and Immunology ; Animal vaccines ; Animals ; Antibody Formation ; Applied microbiology ; Bacteriology ; BCG Vaccine - immunology ; Biodegradation ; Biological and medical sciences ; Cell Proliferation ; Cell Wall Skeleton - immunology ; Cell Wall Skeleton - pharmacology ; Cell-wall skeleton ; Enzyme-Linked Immunosorbent Assay ; Ethyldimethylaminopropyl Carbodiimide - pharmacology ; Freund's Adjuvant - immunology ; Fundamental and applied biological sciences. Psychology ; Immune response ; Immune system ; Immunization ; Immunogenicity ; Immunoglobulin G - blood ; Immunotherapy ; Interferon-gamma - immunology ; Male ; Mice ; Mice, Inbred BALB C ; Microbiology ; Miscellaneous ; Mycobacterium ; Mycobacterium bovis - immunology ; Ovalbumin - immunology ; Proteins ; Spleen - cytology ; Spleen - immunology ; Succinimides - pharmacology ; Th1 ; Th1 Cells - immunology ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vehicles</subject><ispartof>Vaccine, 2010-11, Vol.28 (50), p.7873-7880</ispartof><rights>Elsevier Ltd</rights><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Nov 23, 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c575t-95570ae920ff5e8c96610019896addd998daa3e4b9a0e0c2e83139aae8ae39133</citedby><cites>FETCH-LOGICAL-c575t-95570ae920ff5e8c96610019896addd998daa3e4b9a0e0c2e83139aae8ae39133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1497944959?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23504551$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20937311$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paik, Tae-Hyun</creatorcontrib><creatorcontrib>Lee, Ji-Sook</creatorcontrib><creatorcontrib>Kim, Ki-Hye</creatorcontrib><creatorcontrib>Yang, Chul-Su</creatorcontrib><creatorcontrib>Jo, Eun-Kyeong</creatorcontrib><creatorcontrib>Song, Chang-Hwa</creatorcontrib><title>Mycobacterial cell-wall skeleton as a universal vaccine vehicle for antigen conjugation</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstracts Mycobacterial cell-wall skeleton (CWS) is an immunoactive and biodegradable particulate adjuvant and has been used for immunotherapy in patients with cancer. The CWS of Mycobacterium bovis bacillus Calmette-Guérin (BCG-CWS) was studied as a universal vaccine vehicle for antigen conjugation, to develop potentially effective and safe vaccines. Here, we describe experiments in which protein antigens, such as keyhole limpet haemocyanin (KLH), ovalbumin (OVA) and bovine serum albumin (BSA) were highly efficiently coupled to 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N -hydroxysuccinimide (EDC/NHS)-activated carboxyl groups of BCG-CWS, and tested the immunogenicity of OVA-conjugated BCG-CWS vaccine. We found that a strong immune response was induced in mice immunised with OVA-conjugated BCG-CWS, which was similar to the enhancement of the immune responses in mice immunised with OVA and complete Freund's adjuvant. Covalent conjugation of OVA to BCG-CWS was essential for Th1-skewed immune responses, with prominent expression of IFN-γ. Furthermore, antigen-conjugated BCG-CWS vaccine is simple to manufacture, safe, and easy to use. Our results suggest that mycobacterial CWS as a universal vaccine vehicle for conjugation of a wide variety of antigens constitutes a breakthrough for development of the most promising vaccines for infections, allergic diseases, and cancer.</description><subject>Adjuvant</subject><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Allergy and Immunology</subject><subject>Animal vaccines</subject><subject>Animals</subject><subject>Antibody Formation</subject><subject>Applied microbiology</subject><subject>Bacteriology</subject><subject>BCG Vaccine - immunology</subject><subject>Biodegradation</subject><subject>Biological and medical sciences</subject><subject>Cell Proliferation</subject><subject>Cell Wall Skeleton - immunology</subject><subject>Cell Wall Skeleton - pharmacology</subject><subject>Cell-wall skeleton</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Ethyldimethylaminopropyl Carbodiimide - pharmacology</subject><subject>Freund's Adjuvant - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunization</subject><subject>Immunogenicity</subject><subject>Immunoglobulin G - blood</subject><subject>Immunotherapy</subject><subject>Interferon-gamma - immunology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycobacterium</subject><subject>Mycobacterium bovis - immunology</subject><subject>Ovalbumin - immunology</subject><subject>Proteins</subject><subject>Spleen - cytology</subject><subject>Spleen - immunology</subject><subject>Succinimides - pharmacology</subject><subject>Th1</subject><subject>Th1 Cells - immunology</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vehicles</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkk-LFDEQxYMo7jj6EZQGEU89VpJOd-eiyOI_WPGgordQk65eM5tJr0n3yHx700zrwl72FCh-9SqvXjH2lMOGA69f7TYHtNYF2gjINdAbaOU9tuJtI0uheHufrUDUVVlx-HnGHqW0AwAluX7IzgRo2UjOV-zH56MdtmhHig59Ycn78g96X6Qr8jQOocBUYDEFd6CYMrFMLQ70y1lPRT_EAsPoLikUdgi76RJHN4TH7EGPPtGT5V2z7-_ffTv_WF58-fDp_O1FaVWjxlIr1QCSFtD3ilqr65oDcN3qGruu07rtECVVW41AYAW1kkuNSC2S1FzKNXt50r2Ow--J0mj2Ls0uMNAwJdPqTHEh67tJLrhsVGbX7PktcjdMMWQbhle60VWllc6UOlE2DilF6s11dHuMR8PBzBGZnVmWZeaIDGiTI8p9zxb1abun7n_Xv0wy8GIBMFn0fcRgXbrhpIJKqZl7c-Io7_fgKJpkHQVLnYtkR9MN7s6vvL6lYL0LLg-9oiOlG9cmCQPm63xP8znNEVVCtfIvlI7F9A</recordid><startdate>20101123</startdate><enddate>20101123</enddate><creator>Paik, Tae-Hyun</creator><creator>Lee, Ji-Sook</creator><creator>Kim, Ki-Hye</creator><creator>Yang, Chul-Su</creator><creator>Jo, Eun-Kyeong</creator><creator>Song, Chang-Hwa</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20101123</creationdate><title>Mycobacterial cell-wall skeleton as a universal vaccine vehicle for antigen conjugation</title><author>Paik, Tae-Hyun ; Lee, Ji-Sook ; Kim, Ki-Hye ; Yang, Chul-Su ; Jo, Eun-Kyeong ; Song, Chang-Hwa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c575t-95570ae920ff5e8c96610019896addd998daa3e4b9a0e0c2e83139aae8ae39133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adjuvant</topic><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Allergy and Immunology</topic><topic>Animal vaccines</topic><topic>Animals</topic><topic>Antibody Formation</topic><topic>Applied microbiology</topic><topic>Bacteriology</topic><topic>BCG Vaccine - immunology</topic><topic>Biodegradation</topic><topic>Biological and medical sciences</topic><topic>Cell Proliferation</topic><topic>Cell Wall Skeleton - immunology</topic><topic>Cell Wall Skeleton - pharmacology</topic><topic>Cell-wall skeleton</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Ethyldimethylaminopropyl Carbodiimide - pharmacology</topic><topic>Freund's Adjuvant - immunology</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paik, Tae-Hyun</au><au>Lee, Ji-Sook</au><au>Kim, Ki-Hye</au><au>Yang, Chul-Su</au><au>Jo, Eun-Kyeong</au><au>Song, Chang-Hwa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mycobacterial cell-wall skeleton as a universal vaccine vehicle for antigen conjugation</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2010-11-23</date><risdate>2010</risdate><volume>28</volume><issue>50</issue><spage>7873</spage><epage>7880</epage><pages>7873-7880</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Abstracts Mycobacterial cell-wall skeleton (CWS) is an immunoactive and biodegradable particulate adjuvant and has been used for immunotherapy in patients with cancer. The CWS of Mycobacterium bovis bacillus Calmette-Guérin (BCG-CWS) was studied as a universal vaccine vehicle for antigen conjugation, to develop potentially effective and safe vaccines. Here, we describe experiments in which protein antigens, such as keyhole limpet haemocyanin (KLH), ovalbumin (OVA) and bovine serum albumin (BSA) were highly efficiently coupled to 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N -hydroxysuccinimide (EDC/NHS)-activated carboxyl groups of BCG-CWS, and tested the immunogenicity of OVA-conjugated BCG-CWS vaccine. We found that a strong immune response was induced in mice immunised with OVA-conjugated BCG-CWS, which was similar to the enhancement of the immune responses in mice immunised with OVA and complete Freund's adjuvant. Covalent conjugation of OVA to BCG-CWS was essential for Th1-skewed immune responses, with prominent expression of IFN-γ. Furthermore, antigen-conjugated BCG-CWS vaccine is simple to manufacture, safe, and easy to use. Our results suggest that mycobacterial CWS as a universal vaccine vehicle for conjugation of a wide variety of antigens constitutes a breakthrough for development of the most promising vaccines for infections, allergic diseases, and cancer.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>20937311</pmid><doi>10.1016/j.vaccine.2010.09.083</doi><tpages>8</tpages></addata></record> |
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| ispartof | Vaccine, 2010-11, Vol.28 (50), p.7873-7880 |
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| subjects | Adjuvant Adjuvants, Immunologic - pharmacology Allergy and Immunology Animal vaccines Animals Antibody Formation Applied microbiology Bacteriology BCG Vaccine - immunology Biodegradation Biological and medical sciences Cell Proliferation Cell Wall Skeleton - immunology Cell Wall Skeleton - pharmacology Cell-wall skeleton Enzyme-Linked Immunosorbent Assay Ethyldimethylaminopropyl Carbodiimide - pharmacology Freund's Adjuvant - immunology Fundamental and applied biological sciences. Psychology Immune response Immune system Immunization Immunogenicity Immunoglobulin G - blood Immunotherapy Interferon-gamma - immunology Male Mice Mice, Inbred BALB C Microbiology Miscellaneous Mycobacterium Mycobacterium bovis - immunology Ovalbumin - immunology Proteins Spleen - cytology Spleen - immunology Succinimides - pharmacology Th1 Th1 Cells - immunology Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vehicles |
| title | Mycobacterial cell-wall skeleton as a universal vaccine vehicle for antigen conjugation |
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