integrated enzyme‐linked immunosorbent assay system with an organic light‐emitting diode and a charge‐coupled device for fluorescence detection
A fluorescence detection system for a microfluidic device using an organic light‐emitting diode (OLED) as the excitation light source and a charge‐coupled device (CCD) as the photo detector was developed. The OLED was fabricated on a glass plate by photolithography and a vacuum deposition technique....
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| Veröffentlicht in: | Journal of separation science 2011-10, Vol.34 (20), p.2906-2912 |
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| creator | Nakajima, Hizuru Okuma, Yukiko Morioka, Kazuhiro Miyake, Mayo Hemmi, Akihide Tobita, Tatsuya Yahiro, Masayuki Yokoyama, Daisuke Adachi, Chihaya Soh, Nobuaki Nakano, Koji Xue, Shuhua Zeng, Hulie Uchiyama, Katsumi Imato, Toshihiko |
| description | A fluorescence detection system for a microfluidic device using an organic light‐emitting diode (OLED) as the excitation light source and a charge‐coupled device (CCD) as the photo detector was developed. The OLED was fabricated on a glass plate by photolithography and a vacuum deposition technique. The OLED produced a green luminescence with a peak emission at 512 nm and a half bandwidth of 55 nm. The maximum external quantum efficiency of the OLED was 7.2%. The emission intensity of the OLED at 10 mA/cm2 was 13 μW (1.7 mW/cm2). The fluorescence detection system consisted of the OLED device, two band‐pass filters, a five microchannel poly(dimethylsiloxane) (PDMS) microfluidic device and a linear CCD. The fluorescence detection system was successfully used in a flow‐based enzyme‐linked immunosorbent assay on a PDMS microfluidic device for the rapid determination of immunoglobulin A (IgA), a marker for human stress. The detection limit (S/N=3) for IgA was 16.5 ng/mL, and the sensitivity was sufficient for evaluating stress. Compared with the conventional 96‐well microtiter plate assay, the analysis time and the amounts of reagent and sample solutions could all be reduced. |
| doi_str_mv | 10.1002/jssc.201100429 |
| format | Article |
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The OLED was fabricated on a glass plate by photolithography and a vacuum deposition technique. The OLED produced a green luminescence with a peak emission at 512 nm and a half bandwidth of 55 nm. The maximum external quantum efficiency of the OLED was 7.2%. The emission intensity of the OLED at 10 mA/cm2 was 13 μW (1.7 mW/cm2). The fluorescence detection system consisted of the OLED device, two band‐pass filters, a five microchannel poly(dimethylsiloxane) (PDMS) microfluidic device and a linear CCD. The fluorescence detection system was successfully used in a flow‐based enzyme‐linked immunosorbent assay on a PDMS microfluidic device for the rapid determination of immunoglobulin A (IgA), a marker for human stress. The detection limit (S/N=3) for IgA was 16.5 ng/mL, and the sensitivity was sufficient for evaluating stress. Compared with the conventional 96‐well microtiter plate assay, the analysis time and the amounts of reagent and sample solutions could all be reduced.</description><identifier>ISSN: 1615-9306</identifier><identifier>ISSN: 1615-9314</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.201100429</identifier><identifier>PMID: 21898810</identifier><language>eng</language><publisher>Weinheim: WILEY‐VCH Verlag</publisher><subject>Assaying ; Bandpass filters ; CCD ; Charge coupled devices ; detection limit ; Devices ; Enzyme-Linked Immunosorbent Assay - instrumentation ; Enzyme-Linked Immunosorbent Assay - methods ; filters ; Fluorescence ; Fluorescence detection ; glass ; Humans ; Immunoassay ; immunoglobulin A ; Immunoglobulin A - analysis ; Indicators and Reagents ; Microfluidic Analytical Techniques - instrumentation ; Microfluidic Analytical Techniques - methods ; Microfluidics ; Organic light emitting diodes ; Organic light-emitting diode ; Sensitivity and Specificity ; Silicone resins ; Spectrometry, Fluorescence - instrumentation ; Spectrometry, Fluorescence - methods</subject><ispartof>Journal of separation science, 2011-10, Vol.34 (20), p.2906-2912</ispartof><rights>Copyright © 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5119-a3fd07329391fa90591c5aac4fe53e865de8a73f23d9484caf596349f71ff2df3</citedby><cites>FETCH-LOGICAL-c5119-a3fd07329391fa90591c5aac4fe53e865de8a73f23d9484caf596349f71ff2df3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjssc.201100429$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjssc.201100429$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21898810$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakajima, Hizuru</creatorcontrib><creatorcontrib>Okuma, Yukiko</creatorcontrib><creatorcontrib>Morioka, Kazuhiro</creatorcontrib><creatorcontrib>Miyake, Mayo</creatorcontrib><creatorcontrib>Hemmi, Akihide</creatorcontrib><creatorcontrib>Tobita, Tatsuya</creatorcontrib><creatorcontrib>Yahiro, Masayuki</creatorcontrib><creatorcontrib>Yokoyama, Daisuke</creatorcontrib><creatorcontrib>Adachi, Chihaya</creatorcontrib><creatorcontrib>Soh, Nobuaki</creatorcontrib><creatorcontrib>Nakano, Koji</creatorcontrib><creatorcontrib>Xue, Shuhua</creatorcontrib><creatorcontrib>Zeng, Hulie</creatorcontrib><creatorcontrib>Uchiyama, Katsumi</creatorcontrib><creatorcontrib>Imato, Toshihiko</creatorcontrib><title>integrated enzyme‐linked immunosorbent assay system with an organic light‐emitting diode and a charge‐coupled device for fluorescence detection</title><title>Journal of separation science</title><addtitle>J. Sep. Science</addtitle><description>A fluorescence detection system for a microfluidic device using an organic light‐emitting diode (OLED) as the excitation light source and a charge‐coupled device (CCD) as the photo detector was developed. The OLED was fabricated on a glass plate by photolithography and a vacuum deposition technique. The OLED produced a green luminescence with a peak emission at 512 nm and a half bandwidth of 55 nm. The maximum external quantum efficiency of the OLED was 7.2%. The emission intensity of the OLED at 10 mA/cm2 was 13 μW (1.7 mW/cm2). The fluorescence detection system consisted of the OLED device, two band‐pass filters, a five microchannel poly(dimethylsiloxane) (PDMS) microfluidic device and a linear CCD. The fluorescence detection system was successfully used in a flow‐based enzyme‐linked immunosorbent assay on a PDMS microfluidic device for the rapid determination of immunoglobulin A (IgA), a marker for human stress. The detection limit (S/N=3) for IgA was 16.5 ng/mL, and the sensitivity was sufficient for evaluating stress. Compared with the conventional 96‐well microtiter plate assay, the analysis time and the amounts of reagent and sample solutions could all be reduced.</description><subject>Assaying</subject><subject>Bandpass filters</subject><subject>CCD</subject><subject>Charge coupled devices</subject><subject>detection limit</subject><subject>Devices</subject><subject>Enzyme-Linked Immunosorbent Assay - instrumentation</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>filters</subject><subject>Fluorescence</subject><subject>Fluorescence detection</subject><subject>glass</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>immunoglobulin A</subject><subject>Immunoglobulin A - analysis</subject><subject>Indicators and Reagents</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Microfluidic Analytical Techniques - methods</subject><subject>Microfluidics</subject><subject>Organic light emitting diodes</subject><subject>Organic light-emitting diode</subject><subject>Sensitivity and Specificity</subject><subject>Silicone resins</subject><subject>Spectrometry, Fluorescence - instrumentation</subject><subject>Spectrometry, Fluorescence - methods</subject><issn>1615-9306</issn><issn>1615-9314</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctuEzEUhkcIREthyxIssYBNgi9z8xJFkIIqkAgViI3l2scTpzN2sD2UYcUjsOEFeRIcpUSIBazsY33_p2P9RXGf4DnBmD7dxKjmFJM8lJTfKI5JTaoZZ6S8ebjj-qi4E-MGY9K0HN8ujihpedsSfFz8sC5BF2QCjcB9nQb4-e17b91lnu0wjM5HHy7AJSRjlBOKU0wwoCub1kg65EMnnVWot9065SQMNiXrOqSt15AJjSRSaxm6nVf5cdtnsYbPVgEyPiDTjz5AVODyg4YEKlnv7ha3jOwj3Ls-T4rzF8_fLU5nZ2-WLxfPzmaqIoTPJDMaN4xyxomRHFecqEpKVRqoGLR1paGVDTOUaV62pZKm4jUruWmIMVQbdlI83nu3wX8aISYx2LxL30sHfoyi5ZxQTkuaySf_JAlmmSx5U2f00V_oxo_B5X8IUlVlTUlZ76j5nlLBxxjAiG2wgwxTVoldtWJXrThUmwMPrrXjxQD6gP_uMgN8D1zZHqb_6MSr1Wrxp3y2z9pc75dDVoZLUTesqcT710vxkX0gb09bJpaZf7jnjfRCdsFGcb7KuhJjzLOyYb8AIb3ONA</recordid><startdate>201110</startdate><enddate>201110</enddate><creator>Nakajima, Hizuru</creator><creator>Okuma, Yukiko</creator><creator>Morioka, Kazuhiro</creator><creator>Miyake, Mayo</creator><creator>Hemmi, Akihide</creator><creator>Tobita, Tatsuya</creator><creator>Yahiro, Masayuki</creator><creator>Yokoyama, Daisuke</creator><creator>Adachi, Chihaya</creator><creator>Soh, Nobuaki</creator><creator>Nakano, Koji</creator><creator>Xue, Shuhua</creator><creator>Zeng, Hulie</creator><creator>Uchiyama, Katsumi</creator><creator>Imato, Toshihiko</creator><general>WILEY‐VCH Verlag</general><general>WILEY-VCH Verlag</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>201110</creationdate><title>integrated enzyme‐linked immunosorbent assay system with an organic light‐emitting diode and a charge‐coupled device for fluorescence detection</title><author>Nakajima, Hizuru ; Okuma, Yukiko ; Morioka, Kazuhiro ; Miyake, Mayo ; Hemmi, Akihide ; Tobita, Tatsuya ; Yahiro, Masayuki ; Yokoyama, Daisuke ; Adachi, Chihaya ; Soh, Nobuaki ; Nakano, Koji ; Xue, Shuhua ; Zeng, Hulie ; Uchiyama, Katsumi ; Imato, Toshihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5119-a3fd07329391fa90591c5aac4fe53e865de8a73f23d9484caf596349f71ff2df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Assaying</topic><topic>Bandpass filters</topic><topic>CCD</topic><topic>Charge coupled devices</topic><topic>detection limit</topic><topic>Devices</topic><topic>Enzyme-Linked Immunosorbent Assay - instrumentation</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>filters</topic><topic>Fluorescence</topic><topic>Fluorescence detection</topic><topic>glass</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>immunoglobulin A</topic><topic>Immunoglobulin A - analysis</topic><topic>Indicators and Reagents</topic><topic>Microfluidic Analytical Techniques - instrumentation</topic><topic>Microfluidic Analytical Techniques - methods</topic><topic>Microfluidics</topic><topic>Organic light emitting diodes</topic><topic>Organic light-emitting diode</topic><topic>Sensitivity and Specificity</topic><topic>Silicone resins</topic><topic>Spectrometry, Fluorescence - instrumentation</topic><topic>Spectrometry, Fluorescence - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakajima, Hizuru</creatorcontrib><creatorcontrib>Okuma, Yukiko</creatorcontrib><creatorcontrib>Morioka, Kazuhiro</creatorcontrib><creatorcontrib>Miyake, Mayo</creatorcontrib><creatorcontrib>Hemmi, Akihide</creatorcontrib><creatorcontrib>Tobita, Tatsuya</creatorcontrib><creatorcontrib>Yahiro, Masayuki</creatorcontrib><creatorcontrib>Yokoyama, Daisuke</creatorcontrib><creatorcontrib>Adachi, Chihaya</creatorcontrib><creatorcontrib>Soh, Nobuaki</creatorcontrib><creatorcontrib>Nakano, Koji</creatorcontrib><creatorcontrib>Xue, Shuhua</creatorcontrib><creatorcontrib>Zeng, Hulie</creatorcontrib><creatorcontrib>Uchiyama, Katsumi</creatorcontrib><creatorcontrib>Imato, Toshihiko</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakajima, Hizuru</au><au>Okuma, Yukiko</au><au>Morioka, Kazuhiro</au><au>Miyake, Mayo</au><au>Hemmi, Akihide</au><au>Tobita, Tatsuya</au><au>Yahiro, Masayuki</au><au>Yokoyama, Daisuke</au><au>Adachi, Chihaya</au><au>Soh, Nobuaki</au><au>Nakano, Koji</au><au>Xue, Shuhua</au><au>Zeng, Hulie</au><au>Uchiyama, Katsumi</au><au>Imato, Toshihiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>integrated enzyme‐linked immunosorbent assay system with an organic light‐emitting diode and a charge‐coupled device for fluorescence detection</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. Sep. Science</addtitle><date>2011-10</date><risdate>2011</risdate><volume>34</volume><issue>20</issue><spage>2906</spage><epage>2912</epage><pages>2906-2912</pages><issn>1615-9306</issn><issn>1615-9314</issn><eissn>1615-9314</eissn><abstract>A fluorescence detection system for a microfluidic device using an organic light‐emitting diode (OLED) as the excitation light source and a charge‐coupled device (CCD) as the photo detector was developed. The OLED was fabricated on a glass plate by photolithography and a vacuum deposition technique. The OLED produced a green luminescence with a peak emission at 512 nm and a half bandwidth of 55 nm. The maximum external quantum efficiency of the OLED was 7.2%. The emission intensity of the OLED at 10 mA/cm2 was 13 μW (1.7 mW/cm2). The fluorescence detection system consisted of the OLED device, two band‐pass filters, a five microchannel poly(dimethylsiloxane) (PDMS) microfluidic device and a linear CCD. The fluorescence detection system was successfully used in a flow‐based enzyme‐linked immunosorbent assay on a PDMS microfluidic device for the rapid determination of immunoglobulin A (IgA), a marker for human stress. The detection limit (S/N=3) for IgA was 16.5 ng/mL, and the sensitivity was sufficient for evaluating stress. Compared with the conventional 96‐well microtiter plate assay, the analysis time and the amounts of reagent and sample solutions could all be reduced.</abstract><cop>Weinheim</cop><pub>WILEY‐VCH Verlag</pub><pmid>21898810</pmid><doi>10.1002/jssc.201100429</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of separation science, 2011-10, Vol.34 (20), p.2906-2912 |
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| subjects | Assaying Bandpass filters CCD Charge coupled devices detection limit Devices Enzyme-Linked Immunosorbent Assay - instrumentation Enzyme-Linked Immunosorbent Assay - methods filters Fluorescence Fluorescence detection glass Humans Immunoassay immunoglobulin A Immunoglobulin A - analysis Indicators and Reagents Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Microfluidics Organic light emitting diodes Organic light-emitting diode Sensitivity and Specificity Silicone resins Spectrometry, Fluorescence - instrumentation Spectrometry, Fluorescence - methods |
| title | integrated enzyme‐linked immunosorbent assay system with an organic light‐emitting diode and a charge‐coupled device for fluorescence detection |
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